管纹艳虎天牛形态特征与触角及跗节的超微结构
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摘要
采用光学显微镜和扫描电子显微镜技术,研究了管纹艳虎天牛雌雄成虫外部形态特征、触角和跗节感器种类及其差异。结果表明:管纹艳虎天牛雌虫腹部较雄虫腹部明显粗壮,可作为鉴别雌雄虫的主要形态依据;触角上着生有4类14种感器,即6种锥形感器、4种毛形感器、3种刺形感器和1种B?hm氏鬃毛,其中B?hm氏鬃毛和锥形感器VI仅分布于雄虫触角,锥形感器和刺形感器I型在雌雄虫触角上分布存在差异;雌雄成虫跗节上均着生有3类7种感器,即4种锥形感器、1种毛形感器和2种刺形感器,同种感器在类型、分布和数量上无明显的性二型现象。
In this study, we conducted a comparative study on the morphological characteristics of female and male adults of Rhaphuma horsfieldi as well as sensilla of their respective antennae and tarsi using light microscopy and scanning electron microscopy. Results showed that female abdomen was larger compared to males in R.horsfields, which is recognized as the key morphological identification for this cerambycid beetle. Further, fourteen types of antennal sensilla were observed including 6 types of sensilla basiconica, four types of sensilla trichodea, three types of sensilla chaetica and B?hm bristles. Of these, the BB and SB-VI were present only on male antennae; and the SB and SCh-I were differentially distributed on the antennae of both sexes. In addition,seven types of sensilla were identified on female and male tarsi, consisting of 4 types of SB, one type of ST and 2 types of SCh. No obvious sexual differences were observed on the types, distribution and numbers of the same tarsal sensilla.
In this study, we conducted a comparative study on the morphological characteristics of female and male adults of Rhaphuma horsfieldi as well as sensilla of their respective antennae and tarsi using light microscopy and scanning electron microscopy. Results showed that female abdomen was larger compared to males in R.horsfields, which is recognized as the key morphological identification for this cerambycid beetle. Further, fourteen types of antennal sensilla were observed including 6 types of sensilla basiconica, four types of sensilla trichodea, three types of sensilla chaetica and B?hm bristles. Of these, the BB and SB-VI were present only on male antennae; and the SB and SCh-I were differentially distributed on the antennae of both sexes. In addition,seven types of sensilla were identified on female and male tarsi, consisting of 4 types of SB, one type of ST and 2 types of SCh. No obvious sexual differences were observed on the types, distribution and numbers of the same tarsal sensilla.
引文
[1]云南省林业厅,中国科学院动物研究所.云南森林昆虫[M].昆明:云南科技出版社,1987.
[2]Lopes O,Marques P C,Araujo J.The role of antennae in mate recognition in Phoracantha semipunctata(Coleoptera:Cerambycidae)[J].Journal of Insect Behavior,2005,18(2):243-257.
[3]Mitchell R F,Hughes D T,Luetje C W,et al.Sequencing and characterizing odorant receptors of the cerambycid beetle Megacyllene caryae[J].Insect Biochemistry and Molecular Biology,2012,42(7):499-505.
[4]熊赛.天牛亚科虎天牛族头部和跗节超微形态研究[D].重庆:西南大学,2014.
[5]Wickham J D,Harrison R D,Lu W,et al.Generic lures attract cerambycid beetles in a tropical montane rain forest in southern China[J].Journal of Economic Entomology,2014,107(1):259-267.
[6]Xu W,Papanicolaou A,Zhang H J,et al.Expansion of a bitter taste receptor family in a polyphagous insect herbivore[J].Scientific Reports,2016,6:23666.
[7]De Brito Sanchez M G,Lorenzo E,Su S K,et al.The tarsal taste of honey bees:behavioral and electrophysiological analyses[J].Frontiers in Behavioral Neuroscience,2014,8:25.
[8]徐丽丽.沟胫天牛亚科7种天牛不同虫态触角、下颚须和下唇须的感器研究[D].北京:北京林业大学,2016.
[9]阎雄飞,孙月琴,刘永华,等.光肩星天牛触角感受器的环境扫描电镜观察[J].林业科学,2010,46(11):104-109,191-194.
[10]赵红盈,高宇,马晓乾,等.光肩星天牛足跗节感器超微结构观察[J].中国农学通报,2016,32(17):40-43.
[11]吉帅帅,刘乃勇,赵宁,等.白蜡脊虎天牛雌雄成虫特征差异[J].中国森林病虫,2017,36(4):29-33.
[12]Lopes O,Barata E N,Mustaparta H,et al.Fine structure of antennal sensilla basiconica and their detection of plant volatiles in the eucalyptus woodborer,Phoracantha semipunctata Fabricius(Coleoptera:Cerambycidae)[J].Arthropod Structure&Development,2002,31(1):1-13.
[13]Zhang J,Guan L,Ren B Z.Fine structure and distribution of antennal sensilla of longicorn beetles Leptura arcuata and Leptura aethiops(Coleoptera:Cerambycidae)[J].Annals of the Entomological Society of America,2011,104(4):778-787.
[14]李燕,孟庆繁,宗翔,等.栗山天牛触角感受器超微结构观察[J].北京林业大学学报,2013,35(6):80-86.
[15]Chen J M,Qiao H L,Chen J,et al.Observation of antennal sensilla in Xylotrechus grayii(Coleoptera:Cerambycidae)with scanning electron microscopy[J].Microscopy Research&Technique,2014,77(4):264-273.
[16]王政,孙世伟,孟倩倩,等.咖啡灭字虎天牛触角和足感器的形态与分布观察[J].植物保护,2018,44(3):92-97.
[17]蒋书南,蒲富基,华立中.中国经济昆虫志:第35册:鞘翅目:天牛科)[M].北京:科学出版社,1985.
[18]Schneider D.Insect Antennae[J].Annual Review of Entomology,1964,9(1):103-122.
[19]Crook D J,Higgins R A,Ramaswamy S B.Antennal morphology of the soybean stemborer Dectes texanus texanus Le Conte(Coleoptera:Cerambycidae)[J].Journal of Kansas Entomological Society,2003,76(3):397-405.
[20]马瑞燕,杜家纬.昆虫的触角感器[J].昆虫知识,2000,37(3):179-183.
[21]张晓军,孙伟,张健,等.鞘翅目昆虫触角感器研究进展[J].安徽农业科学,2013,41(7):2932-2935.
[22]Merivee E,Ploomi A,Rahi M,et al.Antennal sensilla of the ground beetle Bembidion properans Steph.(Coleoptera,Carabidae)[J].Micron,2002,33(5):429-440.
[23]田立超,陈力,李竹.虎天牛族中国六新纪录种:鞘翅目:天牛科:天牛亚科[J].动物分类学报,2012,37(2):440-443.
[24]田立超,陈力,李竹.虎天牛族中国四新纪录种:鞘翅目:天牛科)[J].动物分类学报,2013,38(1):200-202.
[25]Li Z,Tian L H,Peng C L,et al.Two newly recorded genera and eight species of Cerambycidae from China(Coleoptera)[J].Entomotaxonomia,2018,40(2):125-130.
[26]Inouchi J,Shibuya T,Matsuzaki O,et al.Distribution and fine structure of antennal olfactory sensilla in Japanese dung beetles,Geotrupes auratus Mtos.(Coleoptera:Geotrupidae)and Copris pecuarius Lew.(Coleoptera:Scarabaeidae)[J].International Journal of Insect Morphology and Embryology,1987,16(2):177-187.
[27]Mac Kay C A,Sweeney J D,Hillier N K.Morphology of antennal sensilla of the brown spruce longhorn beetle,Tetropium fuscum(Fabr.)(Coleoptera:Cerambycidae)[J].Arthropod Structure&Development,2014,43(5):469-475.
[28]Onagbola E O,Meyer W L,Boina D R,et al.Morpho-logical characterization of the antennal sensilla of the Asian citrus psyllid,Diaphorina citri Kuwayama(Hemiptera:Psyllidae),with reference to their probable functions[J].Micron,2008,39(8):1184-1191.
[29]Drechsler P,Federle W.Biomechanics of smooth adhesive pads in insects:influence of tarsal secretion on attachment performance[J].Journal of Comparative Physiology.A,Neuroethology,Sensory,Neural,and Behavioral Physiology,2006,192(11):1213-1222.
[30]Zill S N,Keller B R,Chaudhry S,et al.Detecting substrate engagement:responses of tarsal campaniform sensilla in cockroaches[J].Journal of Comparative Physiology A,2010,196(6):407-420.
[2]Lopes O,Marques P C,Araujo J.The role of antennae in mate recognition in Phoracantha semipunctata(Coleoptera:Cerambycidae)[J].Journal of Insect Behavior,2005,18(2):243-257.
[3]Mitchell R F,Hughes D T,Luetje C W,et al.Sequencing and characterizing odorant receptors of the cerambycid beetle Megacyllene caryae[J].Insect Biochemistry and Molecular Biology,2012,42(7):499-505.
[4]熊赛.天牛亚科虎天牛族头部和跗节超微形态研究[D].重庆:西南大学,2014.
[5]Wickham J D,Harrison R D,Lu W,et al.Generic lures attract cerambycid beetles in a tropical montane rain forest in southern China[J].Journal of Economic Entomology,2014,107(1):259-267.
[6]Xu W,Papanicolaou A,Zhang H J,et al.Expansion of a bitter taste receptor family in a polyphagous insect herbivore[J].Scientific Reports,2016,6:23666.
[7]De Brito Sanchez M G,Lorenzo E,Su S K,et al.The tarsal taste of honey bees:behavioral and electrophysiological analyses[J].Frontiers in Behavioral Neuroscience,2014,8:25.
[8]徐丽丽.沟胫天牛亚科7种天牛不同虫态触角、下颚须和下唇须的感器研究[D].北京:北京林业大学,2016.
[9]阎雄飞,孙月琴,刘永华,等.光肩星天牛触角感受器的环境扫描电镜观察[J].林业科学,2010,46(11):104-109,191-194.
[10]赵红盈,高宇,马晓乾,等.光肩星天牛足跗节感器超微结构观察[J].中国农学通报,2016,32(17):40-43.
[11]吉帅帅,刘乃勇,赵宁,等.白蜡脊虎天牛雌雄成虫特征差异[J].中国森林病虫,2017,36(4):29-33.
[12]Lopes O,Barata E N,Mustaparta H,et al.Fine structure of antennal sensilla basiconica and their detection of plant volatiles in the eucalyptus woodborer,Phoracantha semipunctata Fabricius(Coleoptera:Cerambycidae)[J].Arthropod Structure&Development,2002,31(1):1-13.
[13]Zhang J,Guan L,Ren B Z.Fine structure and distribution of antennal sensilla of longicorn beetles Leptura arcuata and Leptura aethiops(Coleoptera:Cerambycidae)[J].Annals of the Entomological Society of America,2011,104(4):778-787.
[14]李燕,孟庆繁,宗翔,等.栗山天牛触角感受器超微结构观察[J].北京林业大学学报,2013,35(6):80-86.
[15]Chen J M,Qiao H L,Chen J,et al.Observation of antennal sensilla in Xylotrechus grayii(Coleoptera:Cerambycidae)with scanning electron microscopy[J].Microscopy Research&Technique,2014,77(4):264-273.
[16]王政,孙世伟,孟倩倩,等.咖啡灭字虎天牛触角和足感器的形态与分布观察[J].植物保护,2018,44(3):92-97.
[17]蒋书南,蒲富基,华立中.中国经济昆虫志:第35册:鞘翅目:天牛科)[M].北京:科学出版社,1985.
[18]Schneider D.Insect Antennae[J].Annual Review of Entomology,1964,9(1):103-122.
[19]Crook D J,Higgins R A,Ramaswamy S B.Antennal morphology of the soybean stemborer Dectes texanus texanus Le Conte(Coleoptera:Cerambycidae)[J].Journal of Kansas Entomological Society,2003,76(3):397-405.
[20]马瑞燕,杜家纬.昆虫的触角感器[J].昆虫知识,2000,37(3):179-183.
[21]张晓军,孙伟,张健,等.鞘翅目昆虫触角感器研究进展[J].安徽农业科学,2013,41(7):2932-2935.
[22]Merivee E,Ploomi A,Rahi M,et al.Antennal sensilla of the ground beetle Bembidion properans Steph.(Coleoptera,Carabidae)[J].Micron,2002,33(5):429-440.
[23]田立超,陈力,李竹.虎天牛族中国六新纪录种:鞘翅目:天牛科:天牛亚科[J].动物分类学报,2012,37(2):440-443.
[24]田立超,陈力,李竹.虎天牛族中国四新纪录种:鞘翅目:天牛科)[J].动物分类学报,2013,38(1):200-202.
[25]Li Z,Tian L H,Peng C L,et al.Two newly recorded genera and eight species of Cerambycidae from China(Coleoptera)[J].Entomotaxonomia,2018,40(2):125-130.
[26]Inouchi J,Shibuya T,Matsuzaki O,et al.Distribution and fine structure of antennal olfactory sensilla in Japanese dung beetles,Geotrupes auratus Mtos.(Coleoptera:Geotrupidae)and Copris pecuarius Lew.(Coleoptera:Scarabaeidae)[J].International Journal of Insect Morphology and Embryology,1987,16(2):177-187.
[27]Mac Kay C A,Sweeney J D,Hillier N K.Morphology of antennal sensilla of the brown spruce longhorn beetle,Tetropium fuscum(Fabr.)(Coleoptera:Cerambycidae)[J].Arthropod Structure&Development,2014,43(5):469-475.
[28]Onagbola E O,Meyer W L,Boina D R,et al.Morpho-logical characterization of the antennal sensilla of the Asian citrus psyllid,Diaphorina citri Kuwayama(Hemiptera:Psyllidae),with reference to their probable functions[J].Micron,2008,39(8):1184-1191.
[29]Drechsler P,Federle W.Biomechanics of smooth adhesive pads in insects:influence of tarsal secretion on attachment performance[J].Journal of Comparative Physiology.A,Neuroethology,Sensory,Neural,and Behavioral Physiology,2006,192(11):1213-1222.
[30]Zill S N,Keller B R,Chaudhry S,et al.Detecting substrate engagement:responses of tarsal campaniform sensilla in cockroaches[J].Journal of Comparative Physiology A,2010,196(6):407-420.