基于简化基因组测序开发垂穗披碱草(Elymus nutans)SSR标记
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摘要
垂穗披碱草为青藏高原重要生态草及优质多年生牧草。本研究中,通过简化基因组测序技术筛选和鉴定得到了14个多态性的垂穗披碱草基因组SSR标记。14个SSR标记在24个不同垂穗披碱草个体中等位基因数变化范围为2~5,平均每个位点的等位基因数为2.9。近缘种扩增结果显示分别有14对和9对引物在老芒麦和鹅观草属物种中有目标产物扩增,表明14个SSR标记中5个可能是H基因组特异性标记,其余9个可能来自St组或Y组。这些新开发的SSR标记,今后可应用于垂穗披碱草及其近缘种的遗传多样性研究等领域。同时就利用简化基因组测序技术开发SSR标记的效率进行了讨论。
Elymus nutans is an important perennial grass used both for ecological protection and forage in Qinghai-Tibet plateau. In this study, we isolated and characterized 14 novel polymorphic genomic simple sequence repeat(SSR) markers in E. nutans by Reduced-Representation Genome Sequencing(RRGS). The variation range of allele number of 14 SSR markers in 24 E. nutans individuals was between 2 and 5, with an average of 2.9 alleles per SSR. Amplification of relative species showed that 14 and 9 of the markers could be effectively amplified in E. sibiricus and Roegneria species, respectively. This result indicated that 5 of the 14 SSR markers might be H genome-specific markers, and the remaining 9 might be from St genome or Y genome. These newly developed polymorphic SSR markers could be used in the genetic diversity areas of E. nutans and its related species. And the efficiency of developing SSR markers using RRGS was also discussed.
Elymus nutans is an important perennial grass used both for ecological protection and forage in Qinghai-Tibet plateau. In this study, we isolated and characterized 14 novel polymorphic genomic simple sequence repeat(SSR) markers in E. nutans by Reduced-Representation Genome Sequencing(RRGS). The variation range of allele number of 14 SSR markers in 24 E. nutans individuals was between 2 and 5, with an average of 2.9 alleles per SSR. Amplification of relative species showed that 14 and 9 of the markers could be effectively amplified in E. sibiricus and Roegneria species, respectively. This result indicated that 5 of the 14 SSR markers might be H genome-specific markers, and the remaining 9 might be from St genome or Y genome. These newly developed polymorphic SSR markers could be used in the genetic diversity areas of E. nutans and its related species. And the efficiency of developing SSR markers using RRGS was also discussed.
引文
Allen G.C.,Floresvergara M.A.,Krasynanski S.,Kumar S.,and Thompson W.F.,2006,A modified protocol for rapid DNAisolation from plant tissues using cetyltrimethy lammonium bromide,Nature Protocols,1(5):2320-2325
Chen G.C.,Zhou G.Y.,Sun J.,Chen Z.G.,and Lu X.F.,2008Test study on the application of elymus nutans to the vegetation restoration in the gravel-soil-taken field along QinghaiTibet Railway,Zhongguo Tiedao Kexue(China Railway Science),29(5):134-137(陈桂琛,周国英,孙菁,陈志国,卢学峰,2008,采用垂穗披碱草恢复青藏铁路取土场植被的试验研究,中国铁道科学,29(5):134-137)
Chen M.J.,and Jia S.X.,eds.,2002,China forage plants,China Agriculture Press,Beijing,China,pp.119-120(陈默君,贾慎修,编著,2002,中国饲用植物,中国农业出版社,中国,北京,pp.119-120)
Chen S.Y.,Ma X.,Zhang X.Q.,and Chen Z.H.,2009,Genetic variation and geographical divergence in Elymus nutans Griseb.,(Poaceae:Triticeae)from west China,Biochemical Systematics and Ecology,37(6):716-722
Chen S.Y.,Zhang X.Q.,Ma X.,and Huang L.K.,2013,Assessmen ofgenetic diversityand differentiation of Elymus nutans indigenous to Qinghai-Tibet Plateau using simple sequence repeats markers,Canadian Journal of Plant Science,93(6)1089-1096
Chen S.Y.,Chen Z.H.,Ma X.,Zhang X.Q.,Zhu Y.Q.,and Li S.D.2015,Analysis on transferability of wheat SSR marker in Elymus nutans Griseb,Xinan Minzu Daxue Xuebao(Journal of Southwest University for Nationalities),41(3):265-269(陈仕勇,陈智华,马啸,张新全,朱永群,李世丹,2015,小麦SSR标记在垂穗披碱草中的通用性分析,西南民族大学学报,41(3):265-269)
Chen S.Y.,Ma X.,Zhang X.Q.,Chen Z.H.,and Zhou K.,2016The transferability of SSR and EST-SSR marker of different origins in Elymus and Roegeria in the Triticeae(Poaceae),Caoye Xuebao(Acta Prataculturae Sinica),25(2):132-140(陈仕勇,马啸,张新全,陈智华,周凯,2016,不同来源SSR和EST-SSR在披碱草属和鹅观草属物种中的通用性分析,草业学报,25(2):132-140)
Chen Z.H.,Miao J.M.,Zhong J.C.,Ma X.,Chen S.Y.,and Zhang X.Q.,2009,Genetic diversity of wild Elymus nutans germplasm detected by SRAP markers,Caoye Xuebao(Acta Prataculturae Sinica),18(5):192-200(陈智华,苗佳敏,钟金城,马啸,陈仕勇,张新全,2009,野生垂穗披碱草种质遗传多样性的SRAP研究,草业学报,18(5):192-200)
Dou Q.W.,Zhang T.L.,and Tsujimoto H.,2011,Physical mapping of repetitive sequences and genome analysis in six Elymus species by in situ hybridization,Journal of Systematics and Evolution,49(4):347-352
Dou Q.W.,Liu R.J.,and Yu F.,2016,Chromosomal organization of repetitive DNAs in Hordeum bogdanii and H.brevisubulatum,Comparative Cytogenetics,10(4):465-481
Dou Q.W.,Yu F.,Li Y.,Zhao,Y.Y.,and Liu R.J.,2017,High molecular karyotype variation revealed in indigenous Elymus nutans in the Qinghai Plateau,Plant Diversity,39(3):117-122
Glenn T.C.,2011,Field guide to next-generation DNA sequencers,Molecular Ecology Resources,11(5):759-769
Huang G.L.,Liu H.J.,Sun H.B.,Liu Y.,Zhou R.C.,Liao W.B.,and Fan Q.,2017,Development and characterization of 18polymorphic SSR markers for Barthea barthei(Melastomataceae),Applications in Plant Sciences,5(4):1600149
Lei Y.T.,and Dou Q.W.,2012,Distinguishing Elymus nutans from Elymus sibiricus in Qinghai-Tibet using a SSR marker,Caoye Kexue(Pratacultural Science),29(6):937-942(雷云霆,窦全文,2012,青藏高原老芒麦和垂穗披碱草SSR分子标记鉴别,草业科学,29(6):937-942)
Lei Y.T.,Zhao Y.Y.,Yu F.,Li Y.,and Dou Q.W.,2014,Development and characterization of 53 polymorphic genomic-SSRmarkers in Siberian wildrye(Elymus sibiricus L.),Conservation Genetics Resources,6(4):861-864
Liao Z.Y.,Ma Q.Y.,Dai X.G.,Zhang D.F.,and Li S.X.,2014,Microsatellite characters in Juglans regia L.genome by high throughput sequencing technology,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),42(2):65-68(廖卓毅,马秋月,戴晓港,张得芳,李淑娴,2014,利用高通量测序技术分析核桃基因组微卫星特征,东北林业大学学报,42(2):65-68)
Liu F.,Wang Y.S.,Tian X.L.,Mao Z.C.,Zou X.X.,and Xie B.Y.,2012,SSR Mining in pepper(Capsicum annuum L.)transcriptome and the polymorphism analysis,Yuanyi Xuebao(Acta Horticulturae Sinica),39(1):168-174(刘峰,王运生,田雪亮,茆振川,邹学校,谢丙炎,2012,辣椒转录组SSR挖掘及其多态性分析,园艺学报,39(1):168-174)
Lu B.R.,1993,Meiotic studies of Elymus nutans and E.jacquemontii(Poaceae,Triticeae)and their hybrids with Pseudoroegneria spicata and seventeen Elymus species,Plant Systematics and Evolution,186(3-4):193-212
Lu G.P.,and Nie B.,2002,Field evaluation of Elymus nutans under alpine grassland conditions,Caoye Kexue(Pratacultura Science),19(9):13-15(陆光平,聂斌,2002,垂穗披碱草利用价值评价,草业科学,19(12):13-15)
Miller M.R.,Dunham J.P.,Amores A.,Cresko W.A.,and Johnson E.A.,2007,Rapid and cost-effective polymorphism identification and genotyping using restriction site associated DNA(RAD)markers,Genome Research,17(2):240-248
Powell W.,Machray G.C.,and Provan J.,1996,Polymorphism revealed by simple sequence repeats,Trends in Plan Science,1(7):215-222
Rassmann K.,Schl觟tterer C.,and Tautz D.,1991,Isolation of simple-sequence loci for use in polymerase chain reactionbased DNA fingerprinting,Electrophoresis,12(2-3):113-118
Schafleitner R.,Tincopa L.R.,Palomino O.,Rossel G.,Robles RF.,Alagon R.,Rivera C.,Quispe C.,Rojas L.,Pacheco J.A.Solis J.,Cerna D.,Kim J.Y.,Hou J.,and Simon R.,2010,Asweetpotato gene index established by de novo assembly o pyrosequencing and Sanger sequences and mining for genebased microsatellite markers,BMC Genomics,11(1):604
Sun J.Y.,Xin D.W.,Shan C.Y.,Liu C.Y.,Hu G.H.,and Chen QS.,2008,Programming for SSR marker developing software SSR MINING 1.0,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),41(10):3336-3342(孙佳莹,辛大伟,单彩云,刘春燕,胡国华,陈庆山,2008,SSR标记开发软件SSRMINING 1.0的编制,中国农业科学,41(10):3336-3342)
Tautz D.,and Renz M.,1984,Simple sequences are ubiquitous repetitive components of eukaryotic genomes,Nucleic Acids Research,12(10):4127-4138
Wang R.R.C.,and Lu B.R.,2014,Biosystematics and evolutionary relationships of perennial triticeae species revealed by genomic analyses,Journal of Systematics and Evolution,52(6):697-705
Wei L.,Shao W.W.,Zhou H.B.,Ping J.,Li L.M.,and Zhang Y.P.2015,Rapid microsatellite development in Gekko japonicus using sequenced restriction-site associated DNA markers Genetics and Molecular Research,14(4):14119-14122
Wei M.M.,Chen Y.H.,Liu F.Z.,Zhang Y.,and Lian Y.,2016Development of SSR marker for eggplant with transcriptome sequencing data,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),17(6):1082-1091(魏明明,陈钰辉,刘福中,张映,连勇,2016,基于转录组测序的茄子SSR标记开发,植物遗传资源学报,17(6):1082-1091)
Yan X.B.,Wang K.,Zhou H.,Wang C.Z.,and Guo Y.X.,2008Evaluation of universatlity and efficacy of microsatellite markers amplified in elymus taxa from China,Caoye Xuebao(Acta Prataculturae Sinica),17(6):112-120(严学兵,王堃,周禾,王成章,郭玉霞,2008,不同来源SSR标记在我国披碱草属植物的通用性和效率评价,草业学报,17(6):112-120)
Yan X.B,Zhou H.,Wang K.,Wang C.Z.,and Guo Y.X.,2009,The phylogenetic relationships between nine Elymus taxa from China based on three genetic markers,Caoye Xuebao(Acta Prataculturae Sinica),18(3):74-85(严学兵,周禾,王堃,王成章,郭玉霞,2009,我国9种披碱草属植物的系统学关系,草业学报,18(3):74-85)
Yan X.B.,Guo Y.X.,Liu F.Y.,Zhao C.,Liu Q.L.,and Lu B.R.,2010,Population structure affected by excess gene flow in self-pollinating Elymus nutans and E.burchan-buddae(Triticeae:Poaceae),Popul.Ecol.,52(1):233-241
Yan Z.Y.,Zhou Q.P.,Liu W.H.,Yan H.B.,and Zhou G.E.,2014,Evaluation of agronomic characters and production perfomance of six Elymus germplasm from Qinghai-Tibet Plateat,Caoye Kexue(Pratacultural Science),31(1):108-115(闫志勇,周青平,刘文辉,颜红波,周改娥,2014,青藏高原6份披碱草属牧草农艺性状及生产性能评价,草业科学,31(1):108-115)
Yang X.,Liu F.,Zhang Y.,Cheng Y.F.,Xue L.B.,and Chen X.H.,2016,Study on the genetic diversity of eggplant germplasm with SSR markers,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(12):3450-3457(杨旭,刘飞,张宇,成玉富,薛林宝,陈学好,2016,利用SSR标记研究茄子种质资源遗传多样性,基因组学与应用生物学,35(12):3450-3457)
Zane L.,Bargelloni L.,and Patarnello T.,2002,Strategies for microsatellite isolation:a review,Molecular Ecology,11(1):1-16
Zhang C.L.,Guo Z.H.,Zhang X.Q.,Bai S.Q.,Zhang C.B.,Li P.,and Ma X.,2016,Divergence of drooping wildrye(Elymus nutans)and siberian wildrye(E.sibiricus)by SSR marker,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),17(3):416-422(张成林,郭志慧,张新全,白史且,张昌兵,李平,马啸,2016,利用SSR标记对垂穗披碱草和老芒麦进行物种鉴定和遗传变异分析,植物遗传资源学报,17(3):416-422)
Zhang J.B.,Bai S.Q.,Zhang X.Q.,Ma X.,Yan J.J.,Zhang C.B.,and You M.H.,2009,Study on ear characters of elymus nutans griseb,in the northwestern plateau of Sichuan Province,Sichuan Daxue Xuebao(Journal of Sichuan University),46:1505-1509(张建波,白史且,张新全,马啸,鄢家俊,张昌兵,游明鸿,2009,川西北高原不同野生垂穗披碱草种群穗部形态研究,四川大学学报,46(5):1505-1509)
Chen G.C.,Zhou G.Y.,Sun J.,Chen Z.G.,and Lu X.F.,2008Test study on the application of elymus nutans to the vegetation restoration in the gravel-soil-taken field along QinghaiTibet Railway,Zhongguo Tiedao Kexue(China Railway Science),29(5):134-137(陈桂琛,周国英,孙菁,陈志国,卢学峰,2008,采用垂穗披碱草恢复青藏铁路取土场植被的试验研究,中国铁道科学,29(5):134-137)
Chen M.J.,and Jia S.X.,eds.,2002,China forage plants,China Agriculture Press,Beijing,China,pp.119-120(陈默君,贾慎修,编著,2002,中国饲用植物,中国农业出版社,中国,北京,pp.119-120)
Chen S.Y.,Ma X.,Zhang X.Q.,and Chen Z.H.,2009,Genetic variation and geographical divergence in Elymus nutans Griseb.,(Poaceae:Triticeae)from west China,Biochemical Systematics and Ecology,37(6):716-722
Chen S.Y.,Zhang X.Q.,Ma X.,and Huang L.K.,2013,Assessmen ofgenetic diversityand differentiation of Elymus nutans indigenous to Qinghai-Tibet Plateau using simple sequence repeats markers,Canadian Journal of Plant Science,93(6)1089-1096
Chen S.Y.,Chen Z.H.,Ma X.,Zhang X.Q.,Zhu Y.Q.,and Li S.D.2015,Analysis on transferability of wheat SSR marker in Elymus nutans Griseb,Xinan Minzu Daxue Xuebao(Journal of Southwest University for Nationalities),41(3):265-269(陈仕勇,陈智华,马啸,张新全,朱永群,李世丹,2015,小麦SSR标记在垂穗披碱草中的通用性分析,西南民族大学学报,41(3):265-269)
Chen S.Y.,Ma X.,Zhang X.Q.,Chen Z.H.,and Zhou K.,2016The transferability of SSR and EST-SSR marker of different origins in Elymus and Roegeria in the Triticeae(Poaceae),Caoye Xuebao(Acta Prataculturae Sinica),25(2):132-140(陈仕勇,马啸,张新全,陈智华,周凯,2016,不同来源SSR和EST-SSR在披碱草属和鹅观草属物种中的通用性分析,草业学报,25(2):132-140)
Chen Z.H.,Miao J.M.,Zhong J.C.,Ma X.,Chen S.Y.,and Zhang X.Q.,2009,Genetic diversity of wild Elymus nutans germplasm detected by SRAP markers,Caoye Xuebao(Acta Prataculturae Sinica),18(5):192-200(陈智华,苗佳敏,钟金城,马啸,陈仕勇,张新全,2009,野生垂穗披碱草种质遗传多样性的SRAP研究,草业学报,18(5):192-200)
Dou Q.W.,Zhang T.L.,and Tsujimoto H.,2011,Physical mapping of repetitive sequences and genome analysis in six Elymus species by in situ hybridization,Journal of Systematics and Evolution,49(4):347-352
Dou Q.W.,Liu R.J.,and Yu F.,2016,Chromosomal organization of repetitive DNAs in Hordeum bogdanii and H.brevisubulatum,Comparative Cytogenetics,10(4):465-481
Dou Q.W.,Yu F.,Li Y.,Zhao,Y.Y.,and Liu R.J.,2017,High molecular karyotype variation revealed in indigenous Elymus nutans in the Qinghai Plateau,Plant Diversity,39(3):117-122
Glenn T.C.,2011,Field guide to next-generation DNA sequencers,Molecular Ecology Resources,11(5):759-769
Huang G.L.,Liu H.J.,Sun H.B.,Liu Y.,Zhou R.C.,Liao W.B.,and Fan Q.,2017,Development and characterization of 18polymorphic SSR markers for Barthea barthei(Melastomataceae),Applications in Plant Sciences,5(4):1600149
Lei Y.T.,and Dou Q.W.,2012,Distinguishing Elymus nutans from Elymus sibiricus in Qinghai-Tibet using a SSR marker,Caoye Kexue(Pratacultural Science),29(6):937-942(雷云霆,窦全文,2012,青藏高原老芒麦和垂穗披碱草SSR分子标记鉴别,草业科学,29(6):937-942)
Lei Y.T.,Zhao Y.Y.,Yu F.,Li Y.,and Dou Q.W.,2014,Development and characterization of 53 polymorphic genomic-SSRmarkers in Siberian wildrye(Elymus sibiricus L.),Conservation Genetics Resources,6(4):861-864
Liao Z.Y.,Ma Q.Y.,Dai X.G.,Zhang D.F.,and Li S.X.,2014,Microsatellite characters in Juglans regia L.genome by high throughput sequencing technology,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),42(2):65-68(廖卓毅,马秋月,戴晓港,张得芳,李淑娴,2014,利用高通量测序技术分析核桃基因组微卫星特征,东北林业大学学报,42(2):65-68)
Liu F.,Wang Y.S.,Tian X.L.,Mao Z.C.,Zou X.X.,and Xie B.Y.,2012,SSR Mining in pepper(Capsicum annuum L.)transcriptome and the polymorphism analysis,Yuanyi Xuebao(Acta Horticulturae Sinica),39(1):168-174(刘峰,王运生,田雪亮,茆振川,邹学校,谢丙炎,2012,辣椒转录组SSR挖掘及其多态性分析,园艺学报,39(1):168-174)
Lu B.R.,1993,Meiotic studies of Elymus nutans and E.jacquemontii(Poaceae,Triticeae)and their hybrids with Pseudoroegneria spicata and seventeen Elymus species,Plant Systematics and Evolution,186(3-4):193-212
Lu G.P.,and Nie B.,2002,Field evaluation of Elymus nutans under alpine grassland conditions,Caoye Kexue(Pratacultura Science),19(9):13-15(陆光平,聂斌,2002,垂穗披碱草利用价值评价,草业科学,19(12):13-15)
Miller M.R.,Dunham J.P.,Amores A.,Cresko W.A.,and Johnson E.A.,2007,Rapid and cost-effective polymorphism identification and genotyping using restriction site associated DNA(RAD)markers,Genome Research,17(2):240-248
Powell W.,Machray G.C.,and Provan J.,1996,Polymorphism revealed by simple sequence repeats,Trends in Plan Science,1(7):215-222
Rassmann K.,Schl觟tterer C.,and Tautz D.,1991,Isolation of simple-sequence loci for use in polymerase chain reactionbased DNA fingerprinting,Electrophoresis,12(2-3):113-118
Schafleitner R.,Tincopa L.R.,Palomino O.,Rossel G.,Robles RF.,Alagon R.,Rivera C.,Quispe C.,Rojas L.,Pacheco J.A.Solis J.,Cerna D.,Kim J.Y.,Hou J.,and Simon R.,2010,Asweetpotato gene index established by de novo assembly o pyrosequencing and Sanger sequences and mining for genebased microsatellite markers,BMC Genomics,11(1):604
Sun J.Y.,Xin D.W.,Shan C.Y.,Liu C.Y.,Hu G.H.,and Chen QS.,2008,Programming for SSR marker developing software SSR MINING 1.0,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),41(10):3336-3342(孙佳莹,辛大伟,单彩云,刘春燕,胡国华,陈庆山,2008,SSR标记开发软件SSRMINING 1.0的编制,中国农业科学,41(10):3336-3342)
Tautz D.,and Renz M.,1984,Simple sequences are ubiquitous repetitive components of eukaryotic genomes,Nucleic Acids Research,12(10):4127-4138
Wang R.R.C.,and Lu B.R.,2014,Biosystematics and evolutionary relationships of perennial triticeae species revealed by genomic analyses,Journal of Systematics and Evolution,52(6):697-705
Wei L.,Shao W.W.,Zhou H.B.,Ping J.,Li L.M.,and Zhang Y.P.2015,Rapid microsatellite development in Gekko japonicus using sequenced restriction-site associated DNA markers Genetics and Molecular Research,14(4):14119-14122
Wei M.M.,Chen Y.H.,Liu F.Z.,Zhang Y.,and Lian Y.,2016Development of SSR marker for eggplant with transcriptome sequencing data,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),17(6):1082-1091(魏明明,陈钰辉,刘福中,张映,连勇,2016,基于转录组测序的茄子SSR标记开发,植物遗传资源学报,17(6):1082-1091)
Yan X.B.,Wang K.,Zhou H.,Wang C.Z.,and Guo Y.X.,2008Evaluation of universatlity and efficacy of microsatellite markers amplified in elymus taxa from China,Caoye Xuebao(Acta Prataculturae Sinica),17(6):112-120(严学兵,王堃,周禾,王成章,郭玉霞,2008,不同来源SSR标记在我国披碱草属植物的通用性和效率评价,草业学报,17(6):112-120)
Yan X.B,Zhou H.,Wang K.,Wang C.Z.,and Guo Y.X.,2009,The phylogenetic relationships between nine Elymus taxa from China based on three genetic markers,Caoye Xuebao(Acta Prataculturae Sinica),18(3):74-85(严学兵,周禾,王堃,王成章,郭玉霞,2009,我国9种披碱草属植物的系统学关系,草业学报,18(3):74-85)
Yan X.B.,Guo Y.X.,Liu F.Y.,Zhao C.,Liu Q.L.,and Lu B.R.,2010,Population structure affected by excess gene flow in self-pollinating Elymus nutans and E.burchan-buddae(Triticeae:Poaceae),Popul.Ecol.,52(1):233-241
Yan Z.Y.,Zhou Q.P.,Liu W.H.,Yan H.B.,and Zhou G.E.,2014,Evaluation of agronomic characters and production perfomance of six Elymus germplasm from Qinghai-Tibet Plateat,Caoye Kexue(Pratacultural Science),31(1):108-115(闫志勇,周青平,刘文辉,颜红波,周改娥,2014,青藏高原6份披碱草属牧草农艺性状及生产性能评价,草业科学,31(1):108-115)
Yang X.,Liu F.,Zhang Y.,Cheng Y.F.,Xue L.B.,and Chen X.H.,2016,Study on the genetic diversity of eggplant germplasm with SSR markers,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(12):3450-3457(杨旭,刘飞,张宇,成玉富,薛林宝,陈学好,2016,利用SSR标记研究茄子种质资源遗传多样性,基因组学与应用生物学,35(12):3450-3457)
Zane L.,Bargelloni L.,and Patarnello T.,2002,Strategies for microsatellite isolation:a review,Molecular Ecology,11(1):1-16
Zhang C.L.,Guo Z.H.,Zhang X.Q.,Bai S.Q.,Zhang C.B.,Li P.,and Ma X.,2016,Divergence of drooping wildrye(Elymus nutans)and siberian wildrye(E.sibiricus)by SSR marker,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),17(3):416-422(张成林,郭志慧,张新全,白史且,张昌兵,李平,马啸,2016,利用SSR标记对垂穗披碱草和老芒麦进行物种鉴定和遗传变异分析,植物遗传资源学报,17(3):416-422)
Zhang J.B.,Bai S.Q.,Zhang X.Q.,Ma X.,Yan J.J.,Zhang C.B.,and You M.H.,2009,Study on ear characters of elymus nutans griseb,in the northwestern plateau of Sichuan Province,Sichuan Daxue Xuebao(Journal of Sichuan University),46:1505-1509(张建波,白史且,张新全,马啸,鄢家俊,张昌兵,游明鸿,2009,川西北高原不同野生垂穗披碱草种群穗部形态研究,四川大学学报,46(5):1505-1509)