不同预处理方法对芒草酶解效率的影响
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摘要
将芒草秸秆粉碎,分别采用1%H_2SO_4、1%NaOH、蒸汽爆破(SE)、1%Tween-80和SE+1%Tween-80等5种方法对芒草秸秆粉末进行预处理,然后加入纤维素复合酶进行酶解产糖,通过测定酶解上清液中六碳糖的含量来比较不同预处理方法对芒草酶解效率的影响。结果表明,采用SE+1%Tween-80预处理,芒草酶解效率最高,可达74.45%,较未处理提高了3.75～11.79倍;采用1%H_2SO_4、1%NaOH、SE或1%Tween-80预处理,芒草酶解效率较未处理分别提高了0.55～3.24倍、2.52～5.60倍、1.30～5.97倍和0～0.96倍,其中1%Tween-80预处理对芒草秸秆的酶解几乎没有任何促进作用。
After pulverizing Miscanthus straw,we pretreated the straw powder by using the methods of 1%H_2 SO_4,1%NaOH,steam-explosion(SE),1%Tween-80,and SE+1%Tween-80,and then added complex cellulases to produce glucose by enzymolysis.Moreover,we compared the effects of different pretreatments on the enzymolysis efficiency of Miscanthus through the determination of hexose contents in the supernatants.The results show that the enzymolysis efficiency of Miscanthus can reach 74.45%after pretreatment by SE+1%Tween-80,which increases by 3.75-11.79 times of non-pretreatment.In addition,the enzymolysis efficiency of Miscanthus after pretreatment by 1%H_2 SO_4,1%NaOH,SE,and 1%Tween-80 will increase by 0.55-3.24,2.52-5.60,1.30-5.97,and 0-0.96 times of non-pretreatment,respectively.Among them,pretreatment of 1%Tween-80 has hardly effect on the enzymolysis of Miscanthus.
After pulverizing Miscanthus straw,we pretreated the straw powder by using the methods of 1%H_2 SO_4,1%NaOH,steam-explosion(SE),1%Tween-80,and SE+1%Tween-80,and then added complex cellulases to produce glucose by enzymolysis.Moreover,we compared the effects of different pretreatments on the enzymolysis efficiency of Miscanthus through the determination of hexose contents in the supernatants.The results show that the enzymolysis efficiency of Miscanthus can reach 74.45%after pretreatment by SE+1%Tween-80,which increases by 3.75-11.79 times of non-pretreatment.In addition,the enzymolysis efficiency of Miscanthus after pretreatment by 1%H_2 SO_4,1%NaOH,SE,and 1%Tween-80 will increase by 0.55-3.24,2.52-5.60,1.30-5.97,and 0-0.96 times of non-pretreatment,respectively.Among them,pretreatment of 1%Tween-80 has hardly effect on the enzymolysis of Miscanthus.
引文
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[12] HUANG Y,WEI X Y,ZHOU S G,et al.Steam explosion distinctively enhances biomass enzymatic saccharification of cotton stalks by largely reducing cellulose polymerization degree in G.barbadense and G.hirsutum[J].Bioresource Technology,2015,181:224-230.
[13] SUN D,ALAM A,TU Y Y,et al.Steam-exploded biomass saccharification is predominately affected by lignocellulose porosity and largely enhanced by Tween-80in Miscanthus[J].Bioresource Technology,2017,239:7481.
[2] 石元春.中国可再生能源发展战略研究丛书——生物质能卷[M].北京:中国电力出版社,2008.
[3] 王艳婷,徐正丹,彭良才.植物细胞壁沟槽结构与生物质利用研究展望[J].中国科学:生命科学,2014,44(8):766-774.WANG Y T,XU Z D,PENG L C.Research progress in the groove structures of plant cell walls and biomass utilizations[J].Scientia Sinica:Vitae,2014,44(8):766-774.
[4] 王春燕,王立志,李忠杰,等.黑龙江省野生植物荻的营养分析[J].黑龙江生态工程职业学院学报,2009(2):23-24.
[5] 范希峰,左海涛,侯新村,等.芒和荻作为草本能源植物的潜力分析[J].中国农学通报,2010,26(14):381-387.FAN X F,ZUO H T,HOU X C,et al.Potential of Miscanthus spp.and Triarrhenaspp.as herbaceous energy plants[J].Chinese Agricultural Science Bulletin,2010,26(14):381-387.
[6] LEWANDOWSKI I,CLIFTON-BROWN J C,SCURLOCK J M O,et al.Miscanthus:European experience with a novel energy crop[J].Biomass and Bioenergy,2000,19(4):209-227.
[7] HEATON E A,DOHLEMAN F G,LONG S P.Meeting US biofuel goals with less land:the potential of Miscanthus[J].Global Change Biology,2008,14(9):2000-2014.
[8] XIE G S,PENG L C.Genetic engineering of energy crops:a strategy for biofuel production in China[J].Journal of Integrative Plant Biology,2011,53(2):143-150.
[9] FOSTON M,RAGAUSKAS A J.Biomass characterization:recent progress in understanding biomass recalcitrance[J].Industrial Biotechnology,2012,8(4):191-208.
[10] PENG L C,HOCART C H,REDMOND J W,et al.Fractionation of carbohydrates in Arabidopsis root cell walls shows that three radial swelling loci are specifically involved in cellulose production[J].Planta,2000,211(3):406-411.
[11] XU N,ZHANG W,REN S F,et al.Hemicelluloses negatively affect lignocellulose crystallinity for high biomass digestibility under NaOH and H2SO4pretreatments in Miscanthus[J].Biotechnology for Biofuels,2012,5(1):58-69.
[12] HUANG Y,WEI X Y,ZHOU S G,et al.Steam explosion distinctively enhances biomass enzymatic saccharification of cotton stalks by largely reducing cellulose polymerization degree in G.barbadense and G.hirsutum[J].Bioresource Technology,2015,181:224-230.
[13] SUN D,ALAM A,TU Y Y,et al.Steam-exploded biomass saccharification is predominately affected by lignocellulose porosity and largely enhanced by Tween-80in Miscanthus[J].Bioresource Technology,2017,239:7481.