一株2,2’,4,4’-四溴联苯醚(BDE-47)厌氧降解菌的筛选鉴定及降解特性
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摘要
为去除环境中BDE-47的残留,通过以BDE-47为碳源的选择性培养基驯化,从电子垃圾拆解厂的土壤中分离出了1株厌氧降解BDE-47的纯菌种,命名为XM,并研究其对BDE-47的降解特性.经16S rDNA鉴定,XM属于兼性肠杆菌(Enterobacter sp.),当BDE-47浓度为525μg/L,初始接菌量为7.1×10~5 cells/m L时,培养35 d后降解率为35.8%,降解产物中检测到BDE-28.BDE-47的降解反应符合一级动力学,拟合结果为ln C_t=-0.104t+6.22.选择以铁离子、硝酸根和硫酸根作为降解过程中外加的电子受体,BDE-47的降解率明显提高,分别为49.8%、59.1%和67.3%.以上研究结果表明,菌株XM能够有效地降解BDE-47,在电子垃圾污染的生物修复方面具有较好的参考和应用价值.
In an effort to remove BDE-47 residues from the environment, a bacterial strain that is capable of utilizing BDE-47 as the sole carbon source was isolated and screened from soil collected from an e-waste recycling area in Tianjin to analyze the degradation properties. The strain was preliminarily identified as Enterobacter sp. according to a 16 S r DNA gene sequence analysis. The strain degraded 35.8% of 525 μg/L of BDE-47 in 35 d when the initial concentration of bacteria was 7.1 × 10~5 cells/m L. The product of the biodegradation of BDE-47 was BDE-28. The biodegradation of BDE-47 fit well with first-order kinetics, and its degradation kinetics was ln C_t =-0.104 t + 6.22. With the addition of an electron acceptor, such as Fe~(3+), SO_4~(2-)and NO_3~-, the BDE-47 degradation rate was significantly increased to 49.8%, 59.1%, and 67.3%, respectively. The above results revealed that the strain could degrade BDE-47, which is of importance in the application of environmental bioremediation of BDE-47.
In an effort to remove BDE-47 residues from the environment, a bacterial strain that is capable of utilizing BDE-47 as the sole carbon source was isolated and screened from soil collected from an e-waste recycling area in Tianjin to analyze the degradation properties. The strain was preliminarily identified as Enterobacter sp. according to a 16 S r DNA gene sequence analysis. The strain degraded 35.8% of 525 μg/L of BDE-47 in 35 d when the initial concentration of bacteria was 7.1 × 10~5 cells/m L. The product of the biodegradation of BDE-47 was BDE-28. The biodegradation of BDE-47 fit well with first-order kinetics, and its degradation kinetics was ln C_t =-0.104 t + 6.22. With the addition of an electron acceptor, such as Fe~(3+), SO_4~(2-)and NO_3~-, the BDE-47 degradation rate was significantly increased to 49.8%, 59.1%, and 67.3%, respectively. The above results revealed that the strain could degrade BDE-47, which is of importance in the application of environmental bioremediation of BDE-47.
引文
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4管玉峰,涂秀云,吴宏海.珠江入海口水体中多溴联苯醚及其来源分析[J].生态环境学报,2011,20(3):474-479[Guan YF,Tu XY,Wu HH.Distribution and source of polybrominated diphenyl ethers(PBDEs)in riverine water of the Pearl River Delta[J].Ecol Environ Sci,2011,20(3):474-479]
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7 Sun Y,Yuan GL,Li J,Li JC,Wang GH.Polybrominated diphenyl ethers in surface soils near the Changwengluozha glacier of central Tibetan Plateau,China[J].Sci Total Environ,2015,511:399-406
8 Yang C,Harrad S,Abdallah MA,Desborough J,Rose NL,Turner SD,Davidson TA,Goldsmith B.Polybrominated diphenyl ethers(PBDEs)in English freshwater lakes,2008-2012[J].Chemosphere,2014,110:41-47
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10 Liu D,Wu SM,Zhang Q,Guo M,Cheng J,Zhang SH,Yao C,Chen JQ.Occurrence,spatial distribution,and ecological risks of typical hydroxylated polybrominated diphenyl ethers in surface sediments from a large freshwater lake of China[J].Environ Sci Pollut Res,2017,24:5773-5780
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15 Yang M,Qi H,Jia HL,Ren NQ,Ding YS,Ma WL,Liu LY,Hung H,Sverko E,Li YF.Polybrominated diphenyl ethers in air across China:levels,compositions,and gas-particle partitioning[J].Environ Sci Technol,2013,47:8978-8984
16 Mackintosh,SA,Wallace JS,Gross MS,Navarro DD,Perez-Fuentetaja A,Alaee M,Montecastro D,Aga DS.Review on the occurrence and profiles of polybrominated diphenyl ethers in the Philippines[J].Environ Int,2015,85:314-326
17 Whalen MM,Hurd T.Tetrabromobishenol A decreases cell-surface proteins involved in human natral killer(NK)cell-dependant target cell lysis[J].J Immunotoxicol,2011,8(3):393-397
18 Chen A,Yolton K,Rauch SA,Webster GM,Hornung R,Sjoin A,Dietrich KN,Lanphear BP.Prenatal polybrominated diphenyl ether exposures and neurodevelopment in U.S.children through 5 years of age:the HOME study[J].Environ Health Perspect,2014,122(8):856-862
19 Zhao XS,Wang ST,Li DM,You H,Ren X.Effects of percholorate on BDE-47-induced alteration thyroid hormone and gene expression of in the hypothalamus-pituitary-thyroid axis in zebrafish larvae[J].Environ Toxicol Pharmacol,2013,36(3):1176-1185
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28 Wang M,Wang H,Zhang R,Ma M,Mei K,Fang F,Wang X.Photolysis of low-brominated diphenyl ethers and their reactive oxygen speciesrelated reaction mechanisms in an aqueous system[J].PLoS ONE,2015,10:e0135400
29 Li L,Chang W,Wang Y,Ji H,Chen C,Ma W,Zhao J.Rapid,photocatalytic,and deep debromination of polybrominated diphenyl ethers on Pd-TiO2:intermediates and pathways[J].Chemistry,2014,20:11163-11170
30 Stiborova H,Vrkoslavova J,Lovecka P,Pukabova J,Haislova P,Haislova J,Demnerova K.Aerobic biodegradation of selected polybrominated diphenyl ethers(PBDEs)in wastewater sewage sludge[J].Chemosphere,2015,118:315-321
31 Wang LQ,Li Y,Zhang W,Niu L,Du J,Cai W,Wang J.Isolation and characterization of two novel psychrotrophic decabromodiphenyl etherdegrading bacteria from river sediments[J].Environ Sci Pollut Res,2016,23(11):10371-10381
32 Stiborova H,Vrkoslavova J,Lovecka P,Pulkrabova J,Hradkova P,Hajslova J,Demnerova K.Aerobic biodegradation of selected polybrominated diphenyl ethers(PBDEs)in wastewater sewage sludge[J].Chemosphere,2015,118:315-321
33 Yang CW,Huang HW,Chao WL,Chang BV.Bacterial communities associated with aerobic degradation of polybrominated diphenyl ethers from river sediments[J].Environ Sci Pollut Res Int,2015,22(5):3810-3819
34 Xu G,Wang J,Lu M.Complete debromination of decabromodiphenyl ether using the integration of Dehalococcoides sp.strain CBDB1 and zero-valent iron[J].Chemosphere,2014,117:455-461
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