响应面法优化生防蜡样芽孢杆菌BQ-4发酵培养基
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摘要
以从植物根际分离筛选的生防蜡样芽孢杆菌BQ-4菌株为研究对象,采用单因素试验和Box-Behnken中心组合试验研究了蔗糖、豆饼粉和硫酸铵3个变量对芽孢产量的影响,建立了二次回归方程,通过响应面法分析优化出了最佳的发酵培养基配方,为BQ-4菌株的工厂化生产提供技术支持。结果表明:蔗糖、豆饼粉和硫酸铵的最佳浓度分别为21.67、39.80和19.95 g/L,经试验验证在此条件下BQ-4菌株的芽孢产量达29.36×10~8CFU/mL。
Biocontrol Bacillus cereus BQ-4 strain which screened form the soil of plant rhizosphere was used as material and it's fermentation medium was investigated by using single-factor and Box-Behnken design. The effects of sugar,patoto powder and( NH_4)_2SO_4 were evaluated. Results showed that optimal concentrations of sugar,patoto powder and( NH_4)_2 SO_4 were 21. 67、39. 80 and 19. 95 g/L. In this condition,endospore yeild of BQ-4 strain was 29. 36 × 10~8 CFU/mL.
Biocontrol Bacillus cereus BQ-4 strain which screened form the soil of plant rhizosphere was used as material and it's fermentation medium was investigated by using single-factor and Box-Behnken design. The effects of sugar,patoto powder and( NH_4)_2SO_4 were evaluated. Results showed that optimal concentrations of sugar,patoto powder and( NH_4)_2 SO_4 were 21. 67、39. 80 and 19. 95 g/L. In this condition,endospore yeild of BQ-4 strain was 29. 36 × 10~8 CFU/mL.
引文
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[14]康浩,张焱能,张贺,等.生防芽孢杆菌对3种杧果镰刀菌的抑菌效果评价[J].中国南方果树,2017,46(1):55-58.
[15]王鸿磊,王红艳,丁强,等.响应面法优化双孢菇菇柄多糖的提取工艺研究[J].北方园艺,2010(22):163-166.WANG Honglei,WANG Hongyan,DING Qiang,et al.Study on optimization of extraction of polysaccharide from the root of agaricus bisporus stipe using response surface method[J].Northern Horticulture,2010(22):163-166.
[16]农业部种植业管理司.农业部关于印发《到2020年化肥使用量零增长行动方案》和《到2020年农药使用量零增长行动方案》的通知[EB/OL].2015(2015-03-18).http://jiuban.moa.gov.cn/zwllm/tzgg/tz/201503/t20150318_4444765.htm.
[2]NIU D D,LIU H X,JIANG C H,et al.The plant growth-promoting rhizobacterium Bacillus cereus AR156 induces systemic resistance in Arabidopsis thaliana by simultaneously activating salicylateand jasmonate/ethylene-dependent signaling pathways[J].Molecular Plant-microbe Interactions,2011,24(5):533-542.
[3]齐爱勇,赵绪生,刘大群.芽孢杆菌生物防治植物病害研究现状[J].中国农学通报,2011,27(12):277-280.QI Aiyong,ZHAO Xusheng,LIU Daqun.Research of biological control in plant diseases by Bacillus spp[J].Chinese Agricultural Science Bulleting,2011,27(12):277-280.
[4]焦文沁.蜡样芽孢杆菌A-47增效因子的筛选及增效机制初探[D].北京:中国农业大学,2005.
[5]Wakayama S,Lshikawa F Osihik.Myoocerein a novel antifiaigal peptide antibiotic produced by Bacillus cereus[J].Antimicrobiel Agents and Chemotherapy,1984,26(6):939-940.
[6]裴炎,李先碧,彭红卫,等.抗真菌多肽APS-1的分离纯化与特性[J].微生物学报,1999,39(4):348-349.
[7]Krieg A,Lysenko O.Toxins and enzymes of several species of Bacillus,especially of the B cereus-thuringiensis group[J].Zentralbl Bakteriol Naturwiss,1979,134(1):70-88.
[8]陈志谊,刘永峰,刘邮洲,等.植物病害生防芽孢杆菌研究进展[J].江苏农业学报,2012,28(5):999-1 006.CHEN Zhiyi,LIU Yongfeng,LIU Youzhou,et al.Research progress in biocontrol of Bacillus spp.against plant diseases[J].Jiangsu Journal of Agricultural Sciences,2012,28(5):999-1 006.
[9]管珺,胡永红,杨文革,等.蜡样芽孢杆菌防治植物病虫害的研究进展[J].现代农药,2007,6(4):7-10.GUAN Jun,HU Yonghong,YANG Wenge,et al.Advances in plant diseases and pests control by Bacillus cereus[J].Modern Agrochemicals,2007,6(4):7-10.
[10]王勇,周冬梅,郭坚华.蜡质芽孢杆菌AR156对辣椒的防病促生机理研究[J].植物病理学报.2014,44(2):195-203.WANG Yong,ZHOU Dongmei,GUO Jianhua.Disease preventing and growth promoting mechanisms of Bacillus cereus strain AR156 on pepper[J].Acta Phytopathologica Sinica,2014,44(2):195-203.
[11]周京龙,冯自力,冯鸿杰,等.棉花内生蜡状芽孢杆菌YUPP-10对棉花黄萎病的防治作用及机制[J].中国农业科学,2017,50(14):2 717-2 727.ZHOU Jinglong,FENG Zili,FENG Hongjie,et al.Biocontrol effect and mechanism of cotton endophytic bacterium Bacillus cereus YUPP-10 against verticillium wilt in Gossypium hirsutum[J].Scientia Agricultura Sinica,2017,50(14):2 717-2 727.
[12]柯杨,李勃,齐凡.芽孢杆菌在植物真菌病害防治中的应用[J].保鲜与加工,2012,12(5):39-43,47.KE Yang,LI Bo,QI Fan.Application of bacillus on plant fungal diseases prevention[J].Storage and Process,2012,12(5):39-43,47.
[13]殷继忠,蔡柏岩.生防细菌菌剂修复连作障碍的研究进展[J].中国农学通报,2017,33(21):101-107.YIN Jizhong,CAI Baiyan.Continuous cropping obstacle repair by bio-control bacterium[J].Chinese Agricultural Science Bulletin,2017,33(21):101-107.
[14]康浩,张焱能,张贺,等.生防芽孢杆菌对3种杧果镰刀菌的抑菌效果评价[J].中国南方果树,2017,46(1):55-58.
[15]王鸿磊,王红艳,丁强,等.响应面法优化双孢菇菇柄多糖的提取工艺研究[J].北方园艺,2010(22):163-166.WANG Honglei,WANG Hongyan,DING Qiang,et al.Study on optimization of extraction of polysaccharide from the root of agaricus bisporus stipe using response surface method[J].Northern Horticulture,2010(22):163-166.
[16]农业部种植业管理司.农业部关于印发《到2020年化肥使用量零增长行动方案》和《到2020年农药使用量零增长行动方案》的通知[EB/OL].2015(2015-03-18).http://jiuban.moa.gov.cn/zwllm/tzgg/tz/201503/t20150318_4444765.htm.