光波导共振技术结合比色法检测葡萄糖溶液的浓度
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摘要
利用比色法结合双面金属包覆波导构建了一种高灵敏度的光波导振荡场传感器.将葡萄糖溶液与氧化酶试剂混合,使葡萄糖溶液显色并注入波导结构,采用波长为650 nm的激光入射于波导表面,经角度扫描产生衰减全反射(ATR)吸收峰,通过ATR吸收峰的极小值来检测葡萄糖溶液的浓度变化.实验结果表明,该技术对葡萄糖溶液浓度的分辨率达到111 nmol/mL,其灵敏度高于分光光度计.
Glucose is a major component of the carbohydrates in the bodies of animals and plants,and it is very important to determine its quantitative in many areas.In this work,the colorimetric method combined with a double-sided metal coated waveguide was used to constitute a high sensitive oscillation field optical waveguide sensor.The glucose solution was colored by mixing with the oxidase reagent and then was injected into the waveguide.The attenuated total reflection(ATR) absorption peaks could be obtained through angle scanning,with the 650 nm laser beam incidenting on the surface of the waveguide.And the concentration changes of the glucose solution could be detected according to the minimum value of the absorption peaks.The results show that the resolution to the concentration of solution of this method can reach to 111 nmol/mL.
Glucose is a major component of the carbohydrates in the bodies of animals and plants,and it is very important to determine its quantitative in many areas.In this work,the colorimetric method combined with a double-sided metal coated waveguide was used to constitute a high sensitive oscillation field optical waveguide sensor.The glucose solution was colored by mixing with the oxidase reagent and then was injected into the waveguide.The attenuated total reflection(ATR) absorption peaks could be obtained through angle scanning,with the 650 nm laser beam incidenting on the surface of the waveguide.And the concentration changes of the glucose solution could be detected according to the minimum value of the absorption peaks.The results show that the resolution to the concentration of solution of this method can reach to 111 nmol/mL.
引文
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[2]Lu Z.,China Healthcare Innovation,2007,2(8),76-77(鲁争.中国医疗前沿,2007,2(8),76-77)
[3]Li Q.,Zhang L.W.,Yi H.X.,Du M.,Han X.,Guo C.F.,Li J.Y.,Xue C.H.,Food Science,2012,33(4),162-166(李琦,张兰威,易华西,杜明,韩雪,郭春锋,李婧妍,薛超辉.食品科学,2012,33(4),162-166)
[4]Jia S.H.,Cao X.Q.,Gao J.G.,China Surf.Detergent Cosmetics,2003,33(4),261-263(贾寿华,曹晓群,高吉刚.日用化学工业,2003,33(4),261-263)
[5]Dai X.H.,Qi T.,Yang M.R.,Xu B.,Fang X.,Chem.Anal.Meterage,2008,17(3),78-80(戴新华,齐韬,杨梦瑞,徐蓓,方向.化学分析计量,2008,17(3),78-80)
[6]AQSIQ,SAC,Determination of Glucose in Food Enzyme-colorimetric Method and Enzyme-electrode Method,GB/T 16285-2008,2008-06-25(国家质检总局,国家标准化管理委员会.食品中葡萄糖的测定酶-比色法和酶-电极法,GB/T16285-2008,2008-06-25)
[7]Xu Y.,Zhao K.,Zhang X.Y.,He P.G.,Fang Y.Z.,Chem.J.Chinese Universities,2010,31(4),672-678(徐颖,赵琨,张小燕,何品刚,方禹之.高等学校化学学报,2010,31(4),672-678)
[8]Wang X.D.,Tian X.F.,Ji F.J.,Liu T.M.,Chem.J.Chinese Universities,2012,33(3),458-463(王旭东,田晓丰,季福建,刘铁梅.高等学校化学学报,2012,33(3),458-463)
[9]Bai H.Y.,Wang X.H.,Dai Z.H.,Chinese J.Appl.Chem.,2012,29(6),611-616(白红艳,王秀华,戴志晖.应用化学,2012,29(6),611-616)
[10]Liedberg B.,Nylander C.,Lundstrom I.,Biosens.Bioelectron.,1995,10(8),i-ix
[11]Dudak F.C.,Boyaci I.H.,Biotechnol.J.,2009,4(7),1003-1011
[12]Rich R.L.,Myszka D.G.,J.Mol.Recognit.,2011,24(6),892-914
[13]Tian Y.,Zhang W.S.,Song D.Q.,Cao Y.B.,Liu X.,Zhang H.Q.,Xu H.Y.,Chem.J.Chinese Universities,2004,25(3),441-444(田媛,张维实,宋大千,曹彦波,刘霞,张寒琦,许汉英.高等学校化学学报,2004,25(3),441-444)
[14]Huang F.X.,Su D.C.,Song B.,Wu P.,Zhang J.X.,Xu K.X.,Nanotechnology and Precision Engineering,2010,8(2),132-136(黄福祥,栗大超,宋冰,伍鹏,张晶鑫,徐可欣.纳米技术与精密工程,2010,8(2),132-136)
[15]Tian L.,Qiu J.,Zhou Y.C.,Sun S.G.,Microchim Acta,2010,169(3/4),269-275
[16]Aslan K.,Lakowicz J.R.,Geddes C.D.,Anal.Chem.,2005,77(7),2007-2014
[17]Huang C.J.,Dostalek J.,Sessitsch A.,Knoll W.,Anal.Chem.,2011,83(3),674-677
[18]Skivesen N.,Horvath R.,Thinggaard S.,Larsen N.B.,Pedersen H.C.,Biosens.Bioelectron.,2007,22(7),1282-1288
[19]Horvath R.,Pedersen H.C.,Skivesen N.,Svanberg C.,Larsen N.B.,J.Micromech.Microeng.,2005,15(5),1260-1264
[20]Zourob M.,Mohr S.,Brown B.J.T.,Fielden P.R.,Mconnell M.B.,Goddard N.J.,Anal.Chem.,2005,77(1),232-242
[21]Horvath R.,Pedersen H.C.,Larsen N.B.,Appl.Phys.Lett.,2002,81(12),2166-2168
[22]Lu H.,Cao Z.,Li H.,Shen Q.,Appl.Phys.Lett.,2004,85(20),4579-4581
[23]Xu Z.Q.,Xie W.W.,Shen H.F.,The Medical Journal of Industrial Enterprise,2010,23(4),80(徐正琴,谢文卫,沈红粉.工企医刊,2010,23(4),80)
[24]Deng X.Q.,J.Modern Laboratory Medicine,2002,17(2),9(邓晓琴.现代检验医学杂志,2002,17(2),9)
[25]Li H.G.,Shen Q.S.,Cao Z.Q.,Acta Optica Sinica,2002,22(8),924-926(李红根,沈启瞬,曹庄琪.光学学报,2002,22(8),924-926)
[26]Yuan W.,Yin C.,Li H.,Xiao P.,Cao Z.,J.Opt.Soc.Am.B,2011,28(5),968-971
[27]Cao Z.Q.,Guidewave Optics,Science Press,Beijing,2007,101(曹庄琪.导波光学,北京:科学出版社,2007,101)