空心金属包覆波导检测小球藻溶液浓度的研究
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摘要
[目的]为了研究空心金属包覆波导所构造的光波导振荡场传感器对小球藻溶液浓度的检测灵敏度。[方法]将小球藻溶液注入波导结构中,采用波长为650nm的激光经衰减器准直衰减后直接入射于波导上表面银膜,经角度扫描得到反射率随入射角的变化曲线即衰减全反射吸收(ATR)谱,通过ATR吸收峰极小值(及其同步角)来检测小球藻溶液的浓度。[结果]在0~4 nmol/L范围内吸收峰极小值(及其同步角)与浓度呈较好线性关系。[结论]用该方法检测小球藻溶液浓度是可行的。
[Objective]The research aimed to investigate the concentration of chlorella by using an oscillation field optical waveguide sensor with a hollow-core metal-cladded waveguide( HCMW) structure. [Method] The chlorella solution was injected into the waveguide and a 650 nm incident laser beam was used on the upper silver film at certain optical angles. The attenuated total reflection( ATR) dips was obtained as a function of the laser beam incident angle. The depths( and shifts) of the ATR dips were observable quantities to determine the concentration. [Result] The concentration of chlorella was linearly associated with the minimum value( and the corresponding resonance angle) of the ATR dips in the concentration range of 0- 4 nmol / L. [Conclusion] It was feasible to determine the concentration of chlorella by using our proposed method.
[Objective]The research aimed to investigate the concentration of chlorella by using an oscillation field optical waveguide sensor with a hollow-core metal-cladded waveguide( HCMW) structure. [Method] The chlorella solution was injected into the waveguide and a 650 nm incident laser beam was used on the upper silver film at certain optical angles. The attenuated total reflection( ATR) dips was obtained as a function of the laser beam incident angle. The depths( and shifts) of the ATR dips were observable quantities to determine the concentration. [Result] The concentration of chlorella was linearly associated with the minimum value( and the corresponding resonance angle) of the ATR dips in the concentration range of 0- 4 nmol / L. [Conclusion] It was feasible to determine the concentration of chlorella by using our proposed method.
引文
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[12]WANG Y,HUANG M Z,GUAN X Y.Determination of trace chromium(VI)using a hollow-core metal-cladding optical waveguide sensor[J].Optics Express,2013,21(25):31130-31137.
[13]DAI H L,SANG M H,WANG Y X,et al.Optical detection of glyphosate with a prism coupling hollow-core metal-cladded waveguide[J].Sensors and Actuators A-physical,2014,218:88-93.
[14]管相宇,黄梅珍,汪洋,等.光波导共振技术结合比色法检测葡萄糖溶液的浓度[J].高等学校化学学报,2013,34(4):841-845.
[15]BORN M,WOLF E.Principles of optics:electromagnetic theory of propagation,interference and diffraction of light[M].7thedition.Cambridge:Cambridge University Press,1999.
[2]邱华玲,方桂友,冯玉兰.小球藻在污水处理上的研究进展[J].福建畜牧兽医,2007,29(6):73.
[3]吴海锁,张洪玲,张爱茜,等.小球藻吸附重金属离子的试验研究[J].环境化学,2004,23(2):173-177.
[4]ZHANG S S,LIN C Y,CHEN C L,et al.Urban nutrient recovery from fresh human urine through cultivation of Chlorella sorokiniana[J].Journal of Environmental Management,2014,145:129-136.
[5]KE G,MOU X Q,XU Y,et al.Application of ozonated piggery wastewater for Cultivation of oil-rich Chlorella pyrenoidosa[J].Bioresource Technology,2014,171:285-290.
[6]潘竞洋,赵之旭,吴良柏,等.用分光光度计预测小球藻藻液浓度的研究[J].安徽农业科学,2014,42(25):8504-8505,8618.
[7]吕旭阳,张雯,杨阳,等.分光光度法测定小球藻数量的方法研究[J].安徽农业科学,2009,37(23):11104-11105.
[8]涂波,曹敏,黄茜,等.分光光度法与显微计数法测定微小绿藻生物量的比较[J].西南大学学报:自然科学版,2014,36(8):43-50.
[9]何国梁.一种测定小球藻含量的简易方法——比色法[J].江西医学院学报,1962(2):72-75.
[10]董正臻,董振芳,丁德文.快速测定藻类生物量的方法探讨[J].实验技术,2004,28(11):1-2.
[11]曹庄琪.导波光学[M].北京:科学出版社,2007:98-103,150-165.
[12]WANG Y,HUANG M Z,GUAN X Y.Determination of trace chromium(VI)using a hollow-core metal-cladding optical waveguide sensor[J].Optics Express,2013,21(25):31130-31137.
[13]DAI H L,SANG M H,WANG Y X,et al.Optical detection of glyphosate with a prism coupling hollow-core metal-cladded waveguide[J].Sensors and Actuators A-physical,2014,218:88-93.
[14]管相宇,黄梅珍,汪洋,等.光波导共振技术结合比色法检测葡萄糖溶液的浓度[J].高等学校化学学报,2013,34(4):841-845.
[15]BORN M,WOLF E.Principles of optics:electromagnetic theory of propagation,interference and diffraction of light[M].7thedition.Cambridge:Cambridge University Press,1999.