白屈菜内生真菌的分离鉴定及其代谢产物的抗真菌活性研究
|
摘要
为寻找新型抗真菌活性物质的来源,该研究采用常规真菌分离方法,从药用植物白屈菜的根、茎及叶中共分离得到11株可培养的内生真菌,结合分子生物学与形态学鉴定了其中10株,其中刺盘孢属Colletotrichum 7株(L1,L2,L3,S1,S3,S4,S5),镰刀菌属Fusarium 3株(R1,R2,R3)。抗真菌活性测试结果表明,所有菌株代谢产物对5种常见植物病原菌(苹果炭疽菌、玉米弯孢菌、水稻稻瘟菌、烟草赤星菌和白菜黑斑菌)均表现出一定程度的抗菌活性,部分测试项目抑制率超过60%,其中菌株R1,S2,S3,S4的活性相对较高。该研究丰富了对白屈菜内生菌的认识,同时为新型微生物源抗菌物质的深入研究奠定了基础。
In order to find new source of antifungal agents, eleven cultivable endophytic fungi were isolated from the roots,stems and leaves of Chelidonium majus by traditional method. Seven of them were identified as Colletotrichum(L1, L2, L3, S1, S3, S4, S5), and three of them were identified as Fusarium(R1,R2,R3) by morphological features and molecular biological technology. The antifungal activity test showed that all the tested fungi displayed some inhibitory activity against five common plant pathogens(C. gloeosporioides, Curvularia lunata, Pyricularia oryza, Alternaria alternate and A. brassicae), and their inhibition rate of some test items were over 60%. Among them, R1, S2, S3 and S4 were more potent than others. This study enriches the understanding of endophytes from Ch. majus and provides a basis for the study of new microbial fungicides.
In order to find new source of antifungal agents, eleven cultivable endophytic fungi were isolated from the roots,stems and leaves of Chelidonium majus by traditional method. Seven of them were identified as Colletotrichum(L1, L2, L3, S1, S3, S4, S5), and three of them were identified as Fusarium(R1,R2,R3) by morphological features and molecular biological technology. The antifungal activity test showed that all the tested fungi displayed some inhibitory activity against five common plant pathogens(C. gloeosporioides, Curvularia lunata, Pyricularia oryza, Alternaria alternate and A. brassicae), and their inhibition rate of some test items were over 60%. Among them, R1, S2, S3 and S4 were more potent than others. This study enriches the understanding of endophytes from Ch. majus and provides a basis for the study of new microbial fungicides.
引文
[1] 文屏,李加福,高咏莉,等.两面针中白屈菜红碱等成分的抗炎活性研究[J].今日药学,2018,28(4):217.
[2] 顾悦. 白屈菜质量评价研究[D]. 镇江: 江苏大学, 2010: 4.
[3] 刘楠楠. 白屈菜总生物碱提取、安全性评价及体外抑菌作用研究[D]. 长春: 吉林农业大学, 2008: 3.
[4] Christina A, Christapher V, Bhore S J. Endophytic bacteria as a source of novel antibiotics: an overview[J]. Pharmacogn Rev, 2013, 7(13): 11.
[5] 杨志钧,殷瑜,王志强,等. 一株马兜铃内生真菌Colletotrichum sp.代谢产物中细胞毒活性成分的研究[J]. 天然药物研究与开发, 2012, 24(3): 329.
[6] 黄琪佳,吴敏,黄玉莹,等. 鸢尾内生真菌Fusarium oxysporum YW1的分离鉴定及其次生代谢产物的研究[J]. 华中师范大学学报:自然科学版, 2016, 50(6): 880.
[7] 宋新月,汤冰雪,邱君志,等. 竹叶兰内生真菌的分离鉴定及其抗氧化活性研究[J]. 现代食品科技, 2018, 34(2): 82.
[8] Goryluk A, Rekosz-Burlaga H, B?aszczyk M. Isolation and characterization of bacterial endophytes of Chelidonium majus L[J]. Pol J Microbiol, 2009, 58(4): 355.
[9] 王宁宁,刘璐,刘园园,等. 白屈菜内生真菌合成纳米银的活性及抗肿瘤活性分析[J]. 吉林医学, 2017, 38(11): 2003.
[10] 闵长莉,汪学军,赵梦凡,等. 博落回内生真菌的分离及产血根碱菌株的筛选[J]. 中国中药杂志, 2014, 39(22): 4288.
[11] 刘建玲,陈宝宝,雷毅,等. 半夏产生物碱内生菌的分离及其抑菌活性的初步研究[J]. 西北植物学报, 2010, 30(4): 832.
[12] 魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1979: 468.
[13] 钱一鑫,康冀川,耿坤. 青蒿内生真菌的抗肿瘤抗氧化活性[J]. 菌物研究, 2014, 12(1): 44.
[14] 王营,李浩华,谭国慧,等. 广藿香内生真菌类群分析及其抗菌活性研究[J]. 中国中药杂志, 2017, 42(4): 657.
[15] 耿直. 超声波法提取凹叶厚朴内生真菌代谢产物[J]. 安徽农业科学, 2017, 45(1): 122.
[16] Yang R, Gao Z F, Zhao J Y, et al. New class of 2-aryl-6-chloro-3,4-dihydro- isoquinolinium salts as potential antifungal agents for plant protection: synthesis, bioactivity and structure-activity relationships[J]. J Agric Food Chem, 2015, 63(7): 1906.
[17] 陈旭,曾茜,刘璞玉,等. 贵州小白及内生真菌多样性与产抗病活性物质菌株的筛选[J]. 西南农业学报, 2017, 30(1): 111.
[18] 包丽霞,殷瑜,杨天,等. 北细辛内生真菌的分离鉴定及代谢产物的生物活性[J]. 微生物学杂志, 2010, 30(5): 1.
[2] 顾悦. 白屈菜质量评价研究[D]. 镇江: 江苏大学, 2010: 4.
[3] 刘楠楠. 白屈菜总生物碱提取、安全性评价及体外抑菌作用研究[D]. 长春: 吉林农业大学, 2008: 3.
[4] Christina A, Christapher V, Bhore S J. Endophytic bacteria as a source of novel antibiotics: an overview[J]. Pharmacogn Rev, 2013, 7(13): 11.
[5] 杨志钧,殷瑜,王志强,等. 一株马兜铃内生真菌Colletotrichum sp.代谢产物中细胞毒活性成分的研究[J]. 天然药物研究与开发, 2012, 24(3): 329.
[6] 黄琪佳,吴敏,黄玉莹,等. 鸢尾内生真菌Fusarium oxysporum YW1的分离鉴定及其次生代谢产物的研究[J]. 华中师范大学学报:自然科学版, 2016, 50(6): 880.
[7] 宋新月,汤冰雪,邱君志,等. 竹叶兰内生真菌的分离鉴定及其抗氧化活性研究[J]. 现代食品科技, 2018, 34(2): 82.
[8] Goryluk A, Rekosz-Burlaga H, B?aszczyk M. Isolation and characterization of bacterial endophytes of Chelidonium majus L[J]. Pol J Microbiol, 2009, 58(4): 355.
[9] 王宁宁,刘璐,刘园园,等. 白屈菜内生真菌合成纳米银的活性及抗肿瘤活性分析[J]. 吉林医学, 2017, 38(11): 2003.
[10] 闵长莉,汪学军,赵梦凡,等. 博落回内生真菌的分离及产血根碱菌株的筛选[J]. 中国中药杂志, 2014, 39(22): 4288.
[11] 刘建玲,陈宝宝,雷毅,等. 半夏产生物碱内生菌的分离及其抑菌活性的初步研究[J]. 西北植物学报, 2010, 30(4): 832.
[12] 魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1979: 468.
[13] 钱一鑫,康冀川,耿坤. 青蒿内生真菌的抗肿瘤抗氧化活性[J]. 菌物研究, 2014, 12(1): 44.
[14] 王营,李浩华,谭国慧,等. 广藿香内生真菌类群分析及其抗菌活性研究[J]. 中国中药杂志, 2017, 42(4): 657.
[15] 耿直. 超声波法提取凹叶厚朴内生真菌代谢产物[J]. 安徽农业科学, 2017, 45(1): 122.
[16] Yang R, Gao Z F, Zhao J Y, et al. New class of 2-aryl-6-chloro-3,4-dihydro- isoquinolinium salts as potential antifungal agents for plant protection: synthesis, bioactivity and structure-activity relationships[J]. J Agric Food Chem, 2015, 63(7): 1906.
[17] 陈旭,曾茜,刘璞玉,等. 贵州小白及内生真菌多样性与产抗病活性物质菌株的筛选[J]. 西南农业学报, 2017, 30(1): 111.
[18] 包丽霞,殷瑜,杨天,等. 北细辛内生真菌的分离鉴定及代谢产物的生物活性[J]. 微生物学杂志, 2010, 30(5): 1.