投喂时间影响大菱鲆幼鱼的生长及类胰岛素样生长因子-Ⅰ基因的表达
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摘要
本试验旨在研究投喂时间对大菱鲆幼鱼生长和类胰岛素样生长因子-Ⅰ(IGF-Ⅰ)基因表达的影响。试验设3个组,分别于早上(06:00)、中午(12:00)、傍晚(18:00)3个不同的时间点投喂大菱鲆幼鱼[初始平均体重为(8.95±0.13)g],每个组3个重复,每个重复25尾。养殖试验周期为45 d。结果表明:06:00投喂组大菱鲆幼鱼的增重率和特定生长率显著高于其他2组(P<0.05),3个投喂组间大菱鲆幼鱼的摄食率无显著差异(P>0.05),但06:00投喂组与12:00投喂组的饲料效率显著高于18:00投喂组(P<0.05);06:00投喂组大菱鲆幼鱼肝脏中IG F-ⅠmRNA相对表达量显著高于12:00和18:00投喂组(P<0.05),而投喂时间对大菱鲆幼鱼脑中IGF-ⅠmRNA相对表达量无显著影响(P>0.05)。综上,建议体重8 g左右的大菱鲆幼鱼的投喂时间为06:00。
This experiment was conducted to investigate the effects of feeding time on growth and insulin-like growth factor-Ⅰ( IGF-Ⅰ) expression of juvenile turbot( Scophthalmus maximus). Three different feeding time points [morning( 06: 00),noon( 12: 00) and evening( 18: 00) ] were choose to fed juvenile turbot with the average initial body weight of( 8.95±0.13) g,and the fish were divided into three groups according to the feed time. Each group had three replicates and each replicate had 25 fish. The growth trial lasted for 45 days. The results showed as following: the weight gain rate and specific growth rate of juvenile turbot in 06: 00 feeding group were significantly higher than those in other two groups( P<0.05). No significant difference in the feeding rate among three feeding groups( P>0.05),but the feed efficiency in 06: 00 and 12: 00 feeding groups was significantly higher than that in 18: 00 feeding group( P<0.05). The relative expression level of IGF-Ⅰ mRNA in liver of juvenile turbot in 06: 00 feeding group was significantly higher than that in 12: 00 and 18: 00 feeding groups( P<0.05),while the relative expression level of IGF-Ⅰ mRNA in brain of juvenile turbot was not significant different among three feeding groups( P>0.05). In summary,it is suggested that the optimum feeding time for the juvenile turbot with the body weight about 8 g is 06: 00.
This experiment was conducted to investigate the effects of feeding time on growth and insulin-like growth factor-Ⅰ( IGF-Ⅰ) expression of juvenile turbot( Scophthalmus maximus). Three different feeding time points [morning( 06: 00),noon( 12: 00) and evening( 18: 00) ] were choose to fed juvenile turbot with the average initial body weight of( 8.95±0.13) g,and the fish were divided into three groups according to the feed time. Each group had three replicates and each replicate had 25 fish. The growth trial lasted for 45 days. The results showed as following: the weight gain rate and specific growth rate of juvenile turbot in 06: 00 feeding group were significantly higher than those in other two groups( P<0.05). No significant difference in the feeding rate among three feeding groups( P>0.05),but the feed efficiency in 06: 00 and 12: 00 feeding groups was significantly higher than that in 18: 00 feeding group( P<0.05). The relative expression level of IGF-Ⅰ mRNA in liver of juvenile turbot in 06: 00 feeding group was significantly higher than that in 12: 00 and 18: 00 feeding groups( P<0.05),while the relative expression level of IGF-Ⅰ mRNA in brain of juvenile turbot was not significant different among three feeding groups( P>0.05). In summary,it is suggested that the optimum feeding time for the juvenile turbot with the body weight about 8 g is 06: 00.
引文
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[10]苗淑彦,王际英,张利民,等.鲽形目鱼类摄食节律研究进展[J].齐鲁渔业,2009,26(2):16-18.
[11]孙国祥.流速、温度对封闭循环水养殖大菱鲆摄食效应和动态投喂模型的研究[D].硕士学位论文.青岛:中国科学院研究生院,2011.
[12]MARINHO G,PERES H,CARVALHO A P.Effect of feeding time on dietary protein utilization and growth of juvenile Senegalese sole(Solea senegalensis)[J].Aquaculture Research,2014,45(5):828-833.
[13]陈乃松,周洁,靳利娜,等.禁食对大口黑鲈生长和肝脏IGF-ⅠmRNA表达丰度的影响[J].中国水产科学,2010,17(4):713-720.
[14]华益民,林浩然.营养状况对幼年鲤鱼肝脏IGF-I mRNA表达的影响[J].动物学报,2001,47(1):94-100.
[15]DUAN C M.Nutritional and developmental regulation of insulin-like growth factors in fish[J].The Journal of Nutrition,1998,128(2):306S-314S.
[2]QIN C J,GONG Q,WEN Z Y,et al.Comparative analysis of the liver transcriptome of Pelteobagrus vachellii with an alternative feeding time[J].Comparative Biochemistry and Physiology Part D:Genomics and Proteomics,2017,22:131-138.
[3]HOSSAIN M A R,HAYLOR G S,BEVERIDGE M C M.Effect of feeding time and frequency on the growth and feed utilization of African catfish Clarias gariepinus(Burchell 1822)fingerlings[J].Aquaculture Research,2001,32(12):999-1004.
[4]杨严欧,解绶启,姚峰,等.投喂时间对奥尼罗非鱼活动水平和活动节律的影响[J].水生生物学报,2008,32(1):38-41.
[5]BOUJARD T,LEATHERLAND J F.Circadian rhythms and feeding time in fishes[J].Environmental Biology of Fishes,1992,35(2):109-131.
[6]章力,黄希贵,王德寿.鱼类胰岛素样生长因子(IGF)系统的研究进展[J].动物学杂志,2005,40(2):99-105.
[7]马爱军,王新安,杨志,等.大菱鲆(Scophthalmus maximus)幼鱼生长性状的遗传力及其相关性分析[J].海洋与湖沼,2008,39(5):499-504.
[8]王吉桥,谭克非,张剑诚.大菱鲆养殖理论与技术[M].北京:海洋出版社,2006.
[9]常青,梁萌青.大菱鲆的饲料与投喂[J].饲料工业,2001,22(10):13-14.
[10]苗淑彦,王际英,张利民,等.鲽形目鱼类摄食节律研究进展[J].齐鲁渔业,2009,26(2):16-18.
[11]孙国祥.流速、温度对封闭循环水养殖大菱鲆摄食效应和动态投喂模型的研究[D].硕士学位论文.青岛:中国科学院研究生院,2011.
[12]MARINHO G,PERES H,CARVALHO A P.Effect of feeding time on dietary protein utilization and growth of juvenile Senegalese sole(Solea senegalensis)[J].Aquaculture Research,2014,45(5):828-833.
[13]陈乃松,周洁,靳利娜,等.禁食对大口黑鲈生长和肝脏IGF-ⅠmRNA表达丰度的影响[J].中国水产科学,2010,17(4):713-720.
[14]华益民,林浩然.营养状况对幼年鲤鱼肝脏IGF-I mRNA表达的影响[J].动物学报,2001,47(1):94-100.
[15]DUAN C M.Nutritional and developmental regulation of insulin-like growth factors in fish[J].The Journal of Nutrition,1998,128(2):306S-314S.