中药材桔梗中常量元素和微量元素含量分析
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摘要
为比较不同桔梗品种间各种元素含量的差别,以期更好地对桔梗进行开发利用,以硝酸作消解剂进行微波消解,分别用原子吸收光谱法和电感耦合等离子体发射光谱法测定桔梗中的K、Ca、Na、Mg等4种常量元素以及Cu、Zn、Mn、Fe、Ni、Co、Cr、Cd、Pb、V、Sn等11种微量元素含量,对不同生长年限和不同品种的桔梗中的常量元素和微量元素进行比较分析。结果表明,随着生长年限的增加K元素逐渐减少,Ca元素逐渐增加,Na元素先增加后减少,Mg元素第3年明显增加。对于微量元素,Cu含量随着年限增加不断减少;Fe、Co、Cr、Pb、V含量随着年限增加逐渐增加;Sn含量先增加后减少;其余5种元素均呈现出先减少后增加的变化规律。将2年生粉花桔梗与紫花桔梗进行比较发现,所有15种元素中除Cd含量两者相似外,均是粉花桔梗中含量较高。
The study aims to better develop Platycodon grandiflorum by comparing the elements content ofdifferent varieties. The contents of macroelement K, Ca, Na, Mg and trace element Cu, Zn, Mn, Fe, Ni, Co, Cr,Cd, Pb, V, Sn were determined by atomic absorption spectrometry and inductively coupled plasma-atomicemission spectrometry using nitric acid as the digestion agent for microwave digestion. The macroelements andtrace elements of Platycodon grandiflorum were compared among different growth years and different varieties.The results showed that K element gradually decreased, Ca element gradually increased, Na element firstincreased then decreased with the increase of growth years, and Mg element increased obviously in the thirdyear. For the trace elements, the content of Cu decreased with the increase of growth years, the content of Fe,Co, Cr, Pb, V increased with the increase of growth years, the content of Sn first increased then decreased, andthe content of the other five elements showed a variation of first decrease then increase. The contents of thefifteen elements in P. grandiflorum with pink flowers were significantly higher than those in P. grandiflorumwith purple flowers except the content of Cd which was similar in both varieties.
The study aims to better develop Platycodon grandiflorum by comparing the elements content ofdifferent varieties. The contents of macroelement K, Ca, Na, Mg and trace element Cu, Zn, Mn, Fe, Ni, Co, Cr,Cd, Pb, V, Sn were determined by atomic absorption spectrometry and inductively coupled plasma-atomicemission spectrometry using nitric acid as the digestion agent for microwave digestion. The macroelements andtrace elements of Platycodon grandiflorum were compared among different growth years and different varieties.The results showed that K element gradually decreased, Ca element gradually increased, Na element firstincreased then decreased with the increase of growth years, and Mg element increased obviously in the thirdyear. For the trace elements, the content of Cu decreased with the increase of growth years, the content of Fe,Co, Cr, Pb, V increased with the increase of growth years, the content of Sn first increased then decreased, andthe content of the other five elements showed a variation of first decrease then increase. The contents of thefifteen elements in P. grandiflorum with pink flowers were significantly higher than those in P. grandiflorumwith purple flowers except the content of Cd which was similar in both varieties.
引文
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[21]席晓岚,徐红,季宇飞,等.微波消解ICP-AES测定桔梗中微量元素[J].光谱实验室,2010,27(3):884-886.
[22]张宝贤,刘婷婷,夏峥,等.桔梗不同生育期对大量元素的积累规律[J].西北农业学报,2014,23(2):131-136.
[2]魏建和,杨成民,隋春,等.中药材新品种选育研究现状、特点及策略探讨[J].中国现代中药,2011,9:3-8.
[3]朱京斌,陈庆亮,单成刚,等.桔梗新品种鲁梗2号的选育及栽培技术要点[J].山东农业科学,2012,44(7):127-128.
[4]李婷,徐文珊,李西文,等.中药桔梗的现代药理研究进展[J].中药药理与临床,2013,2:205-208.
[5]郭丽,张村,李丽,等.中药桔梗的研究进展[J].中国中药杂志,2007,32(3):181-185.
[6]贾林,陆金健,卢德赵,等.桔梗多糖的分离纯化与含量测定[J].中国农学通报,2011,27(17):83-86.
[7]陈宝芳,仉劲,毕研文,等.粉花桔梗与紫花桔梗中氨基酸组成与含量的比较[J].农业科技通讯,2016,6:140-142.
[8]周长征,李银发,杨春澎.细辛道地药材与微量元素[J].中草药,2000,31(4):292-295.
[9]吴水生,陈丽,郭素华,等.道地与非道地产区泽泻样品中微量元素的比较[J].福建中医学院学报,2004,14(3):32-33.
[10]田柱萍,何邦平,王小燕,等.中药材的药效与其所含微量元素关系的研究进展[J].微量元素与健康研究,2005,4:54-56.
[11]魏久宁.四川七种地道药材中微量元素的分析研究[J].华西医科大学学报,2001,4:634-635.
[12]董顺福,朱志国,刘洁,等.火焰原子吸收法测定11种中成药钾、钠、铬和镍的含量[J].光谱学与光谱分析,1999,19(3):406.
[13]温慧敏.中药材中无机元素测定方法研究[D].沈阳:沈阳药科大学,2006.
[14]张宁,何邦平,林锦明,等.中药材中微量元素含量测定方法的研究进展[J].微量元素与健康研究,2008,3:58-60.
[15]尹峻雅,丁黎,张正行.中药中的微量元素及其测定方法[J].中国药师,1999,6:321-323.
[16]龙沛霞,王东.原子吸收光谱法分析中药微量元素的研究近况[J].微量元素与健康研究,1997,14(1):59.
[17]李国清,毕研文,陈宝芳,等.中草药桔梗人工栽培研究进展[J].农学学报,2016,7:55-59.
[18]金星华,姚艳红.长白山区野生桔梗与种植桔梗微量元素ICP-MS的对比分析[J].食品科技,2009,34(8):249-251.
[19]张宝贤,刘婷婷,夏峥,等.桔梗不同生育期对大量元素的积累规律[J].西北农业学报,2014,2:131-136.
[20]李松,杨靖民,刘晓坤,尤海涛,任跃英.桔梗吸肥规律的研究[J].吉林农业大学学报,2009(1):15-20.
[21]席晓岚,徐红,季宇飞,等.微波消解ICP-AES测定桔梗中微量元素[J].光谱实验室,2010,27(3):884-886.
[22]张宝贤,刘婷婷,夏峥,等.桔梗不同生育期对大量元素的积累规律[J].西北农业学报,2014,23(2):131-136.