香叶醇联合对丙基苯甲醛抑制肝癌HepG2细胞增殖作用及机制研究
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摘要
目的探讨香叶醇联合对丙基苯甲醛抑制肝癌HepG2细胞增殖的作用及机制。方法采用四甲基偶氮唑蓝(MTT)法检测香叶醇和对丙基苯甲醛单独及联合用药对HepG2细胞的抑制作用,分析不同浓度的香叶醇和对丙基苯甲醛对HepG2细胞增殖的影响,以AnnexinV-FITC/PI双染法结合流式细胞术检测香叶醇与对丙基苯甲醛单独及联合作用对肝癌HepG2细胞凋亡的影响。结果浓度依次递增的香叶醇和对丙基苯甲醛单独作用于HepG2细胞增殖的抑制率分别为(2.33±0.22)%、(3.25±0.08)%、(9.23±0.06)%、(12.00±0.26)%、(6.86±0.23)%、(12.86±0.18)%、(14.43±0.30)%、(26.49±0.50)%,香叶醇和对丙基苯甲醛均对HepG2细胞增殖有一定的抑制作用。联合用药处理HepG2细胞的抑制率较单独用药组明显提高(P<0.05),均表现出协同作用。与对照组比较,单独用药组促进了肝癌HepG2细胞的凋亡,联合用药组与单独用药组相比促凋亡能力更明显。结论香叶醇和对丙基苯甲醛联合应用可能通过成分间的协同作用有效地抑制HepG2细胞增殖并促进其凋亡。
Objective To investigate the effect of geraniol combined with p-propylbenzaldehyde on inhibiting the proliferation of hepatocellular carcinoma HepG2 cells and its mechanism.Methods The inhibitory effects of single geraniol and its combination with p-propylbenzaldehyde on HepG2 cells were detected by adopting the MTT assay;the effects of different concentrations of geraniol and p-propylbenzaldehyde on the proliferation of HepG2 cells were investigated.The effects of single geraniol and its combination with p-propylbenzaldehyde on HepG2 cells were detected by using the AnnexinV-FITC/PI double staining and flow cytometry.Results The inhibitory rates of geraniol and p-propylbenzaldehyde with increasing concentration orders for acting HepG2 cells alone were(2.33±0.22)%,(3.25±0.08)%,(9.23±0.06)%,(12.00±0.26)%,(6.86±0.23)%,(12.86±0.18)%,(14.43±0.30)% and(26.49±0.50)% respectively,and geraniol and ppropylbenzaldehyde had a certain inhibitory effect on HepG2 cell proliferation.The inhibitory rate of combined medication for processing HepG2 cells was significantly increased compared with the single medication,all manifested by synergistic effect.Compared with the control group,the single medication group promoted the apoptosis of hepatocellular carcinoma HepG2 cells and the combined medication group had stronger apoptosispromoting effect compared with the single medication group.Conclusion The combination of geraniol and ppropylbenzaldehyde may effectively inhibit HepG2 cell proliferation and promote apoptosis through the synergistic effects between components.
Objective To investigate the effect of geraniol combined with p-propylbenzaldehyde on inhibiting the proliferation of hepatocellular carcinoma HepG2 cells and its mechanism.Methods The inhibitory effects of single geraniol and its combination with p-propylbenzaldehyde on HepG2 cells were detected by adopting the MTT assay;the effects of different concentrations of geraniol and p-propylbenzaldehyde on the proliferation of HepG2 cells were investigated.The effects of single geraniol and its combination with p-propylbenzaldehyde on HepG2 cells were detected by using the AnnexinV-FITC/PI double staining and flow cytometry.Results The inhibitory rates of geraniol and p-propylbenzaldehyde with increasing concentration orders for acting HepG2 cells alone were(2.33±0.22)%,(3.25±0.08)%,(9.23±0.06)%,(12.00±0.26)%,(6.86±0.23)%,(12.86±0.18)%,(14.43±0.30)% and(26.49±0.50)% respectively,and geraniol and ppropylbenzaldehyde had a certain inhibitory effect on HepG2 cell proliferation.The inhibitory rate of combined medication for processing HepG2 cells was significantly increased compared with the single medication,all manifested by synergistic effect.Compared with the control group,the single medication group promoted the apoptosis of hepatocellular carcinoma HepG2 cells and the combined medication group had stronger apoptosispromoting effect compared with the single medication group.Conclusion The combination of geraniol and ppropylbenzaldehyde may effectively inhibit HepG2 cell proliferation and promote apoptosis through the synergistic effects between components.
引文
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[2]KOJIRO M.Histopathology of liver cancers[J].Best Pract Res Clin Gastroenterol,2005(19):39-62.
[3]YANG Y,YUE Y,RUN W Y,et al.Cytotoxic,apoptotic and antioxidant activity of the essential oil of Amomum tsao-ko[J].Bioresour Technol,2010,101(11):4205-4211.
[4]张琪,杨扬.草果挥发油对肝癌H22荷瘤小鼠的抑瘤作用及机制[J].武汉大学学报(理学版),2015,61(2):179-182.
[5]马涛,辛文锋,张文生,等.三七皂苷R_1对Aβ_(1-42)诱导的SH-SY5Y细胞凋亡的保护作用[J].中国中药杂志,2015,40(2):303-307.
[6]黄真真,张丹玉,师琪,等.北美海篷子三萜皂苷bigelovii E抑制mTOR信号通路诱导乳腺癌MCF-7细胞凋亡[J].中国药理学通报,2017,33(12):1655-1660.
[7]赵行宇,侯以森,刘雅范,等.6-姜烯酚诱导胃癌BGC-823细胞凋亡及其机制研究[J].上海中医药杂志,2018,52(2):84-88.
[8]刘盛楠,邵淑丽,隋文静,等.白藜芦醇诱导肺癌A549细胞凋亡[J].基因组学与应用生物学,2015,34(4):685-691.
[9]CAO L Q,SHAO Z L,PENG H P,et al.Rosiglitazone enhances 5-fluorouracil-induced cell growth inhibition in hepatocellular carcinoma cell line Hep3B[J].Chin J Cancer,2010,29(8):741-746.
[10]MING Z J,HU Y,QIU Y H,et al.Synergistic effects of beta-aescin and 5-fluorouracil in human hepatocellular carcinoma SMMC-7721cells[J].Phytomedicine,2010,17(8/9):575-580.
[11]李可欣,马浩然,张男男,等.肠道菌群在肝癌中的作用研究进展[J].实用医学杂志,2018,34(9):1575-1578.
[12]魏矿荣,彭侠彪,梁智恒,等.全球肝癌流行概况[J].中国肿瘤,2015,24(8):621-630.
[13]贺珊,廖长秀.中药治疗肝癌机制的研究进展[J].中成药,2017,39(1):155-160.
[14]宋慧娴,乔飞,邵铭.中医药治疗原发性肝癌的研究进展[J].临床肝胆病杂志,2016,32(1):174-177.
[15]戚益铭,吴霜霜,沈敏鹤,等.中医药治疗原发性肝癌研究述评[J].中医学报,2015,30(1):14-16.
[16]张松,孙丽,李悦.香叶醇在医学领域的应用研究进展[J].西北药学杂志,2017,32(1):124-126.