一种快速测定饲料中粗蛋白质方法的研究
|
摘要
通过对比试验,获得一种经过短时消化即可快速测定饲料原料和成品中粗蛋白质含量且普适性好的检测方法。以国标法作对照,利用过氧化氢和硫酸钾代替国标中的混合催化剂,在420℃下消化0.5h,再经过蒸馏、滴定,便可快速测出样品中粗蛋白质的含量。结果表明,该方法检测结果准确、稳定性好,且与国标法一致。
Through contrast experiment,we obtained an improved method,by which we could test the crude protein content in feed raw materials and finished products after a short time digestion.Compared with international standard method,we used hydrogen peroxide and potassium sulfate,instead of the mixed catalyst in international standard method,to carry on the experiment.Digestion for 0.5 hunder 420℃,the samples was distillated and then titrated,the content of crude protein in the sample could be quickly measured.The results showed that the method was accurate,stable and consistent with the national standard method.
Through contrast experiment,we obtained an improved method,by which we could test the crude protein content in feed raw materials and finished products after a short time digestion.Compared with international standard method,we used hydrogen peroxide and potassium sulfate,instead of the mixed catalyst in international standard method,to carry on the experiment.Digestion for 0.5 hunder 420℃,the samples was distillated and then titrated,the content of crude protein in the sample could be quickly measured.The results showed that the method was accurate,stable and consistent with the national standard method.
引文
[1]李兴芳,王士长,李广平.饲料蛋白质不同测定方法的比较[J].饲料工业,2003,24(8):37-38.
[2]严梅荣.饲料粗蛋白快速测定法研究[J].粮食与饲料工业,2001(10):43-44.
[3]王莹利,张玲宏.鲤鱼配合饲料蛋白质含量检测与分析[J].河南水产,2011(3):39-40.
[4]朱宇旌,郑兰宇,张勇,等.饲料中蛋白质含量测定方法的比较[J].粮食与饲料工业,2011(1):59-62.
[5]李宁.几种蛋白质测量方法的比较[J].山西农业大学学报,2006,26(2):132-134.
[6]王潍波.不同测定方法对饲料粗蛋白测定结果影响的研究[J].饲料与畜牧:新饲料,2008(12):41-45.
[7]李晴媛,徐晓娜.饲料中粗蛋白含量的测定方法[J].中国畜牧兽医文摘,2013,29(2):197-198.
[8]韩丹.浅谈影响饲料中粗蛋白测定准确度的几个因素[J].现代畜牧兽医,2010(5):36-37.
[2]严梅荣.饲料粗蛋白快速测定法研究[J].粮食与饲料工业,2001(10):43-44.
[3]王莹利,张玲宏.鲤鱼配合饲料蛋白质含量检测与分析[J].河南水产,2011(3):39-40.
[4]朱宇旌,郑兰宇,张勇,等.饲料中蛋白质含量测定方法的比较[J].粮食与饲料工业,2011(1):59-62.
[5]李宁.几种蛋白质测量方法的比较[J].山西农业大学学报,2006,26(2):132-134.
[6]王潍波.不同测定方法对饲料粗蛋白测定结果影响的研究[J].饲料与畜牧:新饲料,2008(12):41-45.
[7]李晴媛,徐晓娜.饲料中粗蛋白含量的测定方法[J].中国畜牧兽医文摘,2013,29(2):197-198.
[8]韩丹.浅谈影响饲料中粗蛋白测定准确度的几个因素[J].现代畜牧兽医,2010(5):36-37.