响应面优化调中剂木香内酯提取工艺
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摘要
以木香烃内酯和去氢木香内酯总提取率为评价指标,优化调中剂(木香、山楂、北沙参、川穹组成)中木香内酯超声提取工艺,采用紫外-分光光度法、高效液相色谱法、非水滴定法确定最佳工艺下各成分含量。结果表明:最佳提取条件为液料比10.6∶1(m L/g)、超声时间41 min、超声温度48℃,一副调中剂提取液中含木香烃内酯109.20 mg、去氢木香内酯51.00 mg、黄酮624.77 mg、川穹嗪0.31 mg。该优化提取工艺结果可靠,可用于指导日常用药。
With total extraction yield of costunolide and dehydrocostus lactone as index, the ultrasoud method of extracting costunolide and dehydrocostus lactone from mediator(Costustoot, Crataegus, Coastal glehnia root, Ligusticum chuanxiong Hort)was optimized by response surface design. UVspectrophotometry, high performance liquid chromatography, non-aqueous titration were used to detect the extract composition obained under the optimum conditions. The results showed that the optimum conditions were as follows: the liquid to material ratio was 10.6 ∶ 1(m L/g), the ultrasound time was 41 min and the ultrasound temperature was 48 ℃, the conents of costunolide, dehydrocostus lactone, flavonoidsand and ligustrazine were 109.20, 51.00, 624.77, 0.31 mg, respectively. The extraction process was reliable, and it could provide guide for using medicine daily.
With total extraction yield of costunolide and dehydrocostus lactone as index, the ultrasoud method of extracting costunolide and dehydrocostus lactone from mediator(Costustoot, Crataegus, Coastal glehnia root, Ligusticum chuanxiong Hort)was optimized by response surface design. UVspectrophotometry, high performance liquid chromatography, non-aqueous titration were used to detect the extract composition obained under the optimum conditions. The results showed that the optimum conditions were as follows: the liquid to material ratio was 10.6 ∶ 1(m L/g), the ultrasound time was 41 min and the ultrasound temperature was 48 ℃, the conents of costunolide, dehydrocostus lactone, flavonoidsand and ligustrazine were 109.20, 51.00, 624.77, 0.31 mg, respectively. The extraction process was reliable, and it could provide guide for using medicine daily.
引文
[1]高庄存,高翔宇.显著升高血清高密度脂蛋白浓度的中药复合组方和制备方法:103405538[P].2013-11-27.
[2]FRANCESEHINI G.Epidemiologie evidence for high-density lipoprotein cholesterol as a risk factor for coronary artery disease[J].The American Journal of Cardiology,2001,88(12):9-13.
[3]MA S,MA C H.Recent developments in the effects of nitric oxidedonating statins on cardiovascular disease through regulation of tetrahydrobiopterin and nitric oxide[J].Vascular Pharmacology,2014,63(2):63-70.
[4]FERNANDEZ A B,KARAS R H,ALSHEIKH-ALI A A,et al.Statins and interstitial lung disease:A systematic review of the literature and of food and drug administration adverse event reports[J].Chest,2008,134(4):824-830.
[5]魏华,彭勇,马国需,等.木香有效成分及药理作用研究进展[J].中草药,2012,43(3):613-620.
[6]马锐,吴胜本.中药黄酮类化合物药理作用及作用机制研究进展[J].中国药物警戒,2013,10(5):286-290.
[7]魏芸,彭小春.川穹嗪对血管内皮细胞损伤的保护作用[J].长江大学学报(自科版)医学卷,2007,4(3):224-232.
[8]赵明明,陈宇婷,黄帮蕊,等.HPLC法同时测定好胃来软胶囊中木香烃内酯和去氢木香内酯的含量[J].中国药房,2016,27(15):2099-2101.
[9]黎海彬.山楂中有效成分含量测定的研究[J].食品科学,2008,29(8):475-477.
[10]刘霞,李伟,范小娜,等.非水溶液滴定法测定普卢利沙星的含量[J].光谱实验室,2010,27(1):177-179.
[11]杨虹,刘璐,张海菊.川穹嗪提取工艺及质量标准研究[J].中国民族民间医药,2009,18(7):24,148.
[2]FRANCESEHINI G.Epidemiologie evidence for high-density lipoprotein cholesterol as a risk factor for coronary artery disease[J].The American Journal of Cardiology,2001,88(12):9-13.
[3]MA S,MA C H.Recent developments in the effects of nitric oxidedonating statins on cardiovascular disease through regulation of tetrahydrobiopterin and nitric oxide[J].Vascular Pharmacology,2014,63(2):63-70.
[4]FERNANDEZ A B,KARAS R H,ALSHEIKH-ALI A A,et al.Statins and interstitial lung disease:A systematic review of the literature and of food and drug administration adverse event reports[J].Chest,2008,134(4):824-830.
[5]魏华,彭勇,马国需,等.木香有效成分及药理作用研究进展[J].中草药,2012,43(3):613-620.
[6]马锐,吴胜本.中药黄酮类化合物药理作用及作用机制研究进展[J].中国药物警戒,2013,10(5):286-290.
[7]魏芸,彭小春.川穹嗪对血管内皮细胞损伤的保护作用[J].长江大学学报(自科版)医学卷,2007,4(3):224-232.
[8]赵明明,陈宇婷,黄帮蕊,等.HPLC法同时测定好胃来软胶囊中木香烃内酯和去氢木香内酯的含量[J].中国药房,2016,27(15):2099-2101.
[9]黎海彬.山楂中有效成分含量测定的研究[J].食品科学,2008,29(8):475-477.
[10]刘霞,李伟,范小娜,等.非水溶液滴定法测定普卢利沙星的含量[J].光谱实验室,2010,27(1):177-179.
[11]杨虹,刘璐,张海菊.川穹嗪提取工艺及质量标准研究[J].中国民族民间医药,2009,18(7):24,148.