西藏尼木县一起动物间鼠疫的实验室判定
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摘要
目的对西藏自治区拉萨市尼木县一起动物间鼠疫进行实验室判定。方法取病死喜马拉雅旱獭脏器标本进行细菌学检测和抗原检测,采用实时荧光定量PCR检测鼠疫杆菌核酸(chro392基因)。结果病死喜马拉雅旱獭脏器标本鼠疫杆菌检测阳性、鼠疫F1抗原检测阳性、实时荧光定量PCR检测鼠疫杆菌核酸阳性。结论该病死喜马拉雅旱獭实验室检测为鼠疫杆菌感染所致,据此判定尼木县此次事件为动物间鼠疫疫情。
Objective To determine if plague is infecting different animal species in Nimu County,Lhasa City,Tibet Autonomous Region in the laboratory. Methods Bacterial and antigens were detected in visceral specimens from dead Himalayan marmots.Yersinia pestis nucleic acids(the chro392 gene)were detected using real-time quantitative PCR.Results Visceral specimens from dead Himalayan marmots tested positive for Y.pestis and plague F1 antigen.Y.pestis nucleic acids were detected using real-time quantitative PCR. Conclusion The Himalayan marmots examined in the laboratory died from infection with the plague bacillus,so Nimu County was determined to be the focus of a plague outbreak in different animal species.
Objective To determine if plague is infecting different animal species in Nimu County,Lhasa City,Tibet Autonomous Region in the laboratory. Methods Bacterial and antigens were detected in visceral specimens from dead Himalayan marmots.Yersinia pestis nucleic acids(the chro392 gene)were detected using real-time quantitative PCR.Results Visceral specimens from dead Himalayan marmots tested positive for Y.pestis and plague F1 antigen.Y.pestis nucleic acids were detected using real-time quantitative PCR. Conclusion The Himalayan marmots examined in the laboratory died from infection with the plague bacillus,so Nimu County was determined to be the focus of a plague outbreak in different animal species.
引文
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[2]丛显斌,刘振才.中国鼠疫及防治(2001-2010)[M].长春:吉林科学技术出版社,2014:514
[3]中华人民共和国卫生部.WS 297-2008鼠疫诊断标准[S].北京:中国标准出版社,2008.
[4]何建,魏柏青,祁美英,等.青海省格尔木市鼠疫病原学及流行病学特征分析[J].中国病原生物学杂志,2016,11(1):17-20.
[5]何建,靳娟,张青雯,等.一株鼠毒素基因缺失突变鼠疫耶尔森氏菌株的构建和鉴定[J].中国病原生物学杂志,2016,11(6):513-6.
[6]王梅,唐新元,粱莹,等.鼠疫菌基因组差异编码序列的筛选研究[J].中国病原生物学杂志,2017,12(2):115-8.
[7]李存香,靳娟,吴海莲,等.1978-2014年青海省果洛州鼠疫流行病学分析[J].中国病原生物学杂志,2017,12(7):651-3.
[8]熊浩明,杨晓艳,何建,等.玉树藏族自治州藏系绵羊鼠疫生态流行病学特征分析[J].中国病原生物学杂志,2016,11(10):893-6.
[9]赵小龙,祁美英,辛有全,等.青海省德令哈市鼠疫病原学及流行病学特征分析[J].中国病原生物学杂志,2015,10(12):1097-9.