年龄对不育男性精子DNA碎片率和精子形态的影响
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摘要
目的:评估年龄对男性不育患者精子DNA碎片率(DFI)及精子形态的影响。方法:回顾性分析2014-2018年本院生殖中心7 067例精子形态学参数分析及2015-2017年1 808例精子DFI分析的结果。根据患者年龄将其分为4组:≤25岁,26～35岁,36～45岁,及>45岁。采用单因素方差分析正常形态精子百分率、精子头部畸形百分率以及精子DFI的组间差异,并分析年龄与精子形态学参数及DFI的相关性。结果:随着年龄的增加,正常形态精子百分率和精子头部畸形率降低而精子DFI升高。≤25岁,26～35岁,36～45岁,及>45岁组的正常形态精子百分率分别为(6.14±2.39)%、(6.12±2.48)%、(5.95±2.32)%和(5.48±2.04)%,除≤25岁与26～35岁、≤25岁与36～45岁组间差异无显著性外,其余分组两两间差异均有显著性;各组精子头部畸形率分别为(86.55±5.07)%、(86.16±5.08)%、(85.82±5.27)%和(85.56±5.19)%,除≤25岁与26～35岁、26～35岁与>45岁、36～45岁与>45岁组间差异无显著性外,其余分组两两间差异均有显著性;各组精子DFI分别为(10.44±4.32)%、(12.26±6.84)%、(14.01±7.59)%和(20.08±10.14)%,各组两两间差异均有显著性。不育男性年龄与正常形态精子百分率(r=-0.07,P<0.01)、精子头部畸形率(r=-0.03,P<0.01)显著负相关,而与精子DFI(r=0.26,P<0.01)显著正相关。结论:不育男性的年龄影响DNA完整性和精子正常形态,随着年龄增加,DFI升高而正常形态精子百分率降低,且年龄与DFI及精子正常形态百分率均显著相关。
Objective: To analyze the relationship between infertile male age and sperm morphology, sperm DNA fragment index(DFI). Methods: The sperm morphological parameters of 7 067 infertile male patients from 2014 to 2018 and the sperm DFI of 1 808 from 2015 to 2017 were analyzed retrospectively.The patients were divided into 4 groups according to their age: ≤25 years old, 26-35 years old, 36-45 years old, and >45 years old. Comparison and correlation analysis among their sperm morphological parameters. DFI and age were conducted. Results: With the increase of age, the percentage of sperm with normal morphology and the rate of sperm head deformity decreased while the sperm DFI increased. The percentages of sperm with normal morphology in 4 groups were(6.14 ± 2.39)%,(6.12±2.48)%,(5.95±2.32)% and(5.48±2.04)%, respectively. There were significant differenc-es between the two groups except ≤25 years group and 26～35 years group, ≤25 and 36-45 years group. The rates of sperm head deformity in each group were(86.55 ± 5.07)%,(86.16 ± 5.08)%,(85.82± 5.27)% and(85.56± 5.19)%, respectively. There was no significant difference between ≤25 years group and 26-35 years group, 26-35 years group and >45 years group, 36-45 years group and >45 years group. The sperm DFI of each group was(10.44 ± 4.32)%,(12.26 ± 6.84)%,(14.01± 7.59)% and(20.08± 10.14)%, respectively. The infertile male age was significantly negatively correlated with percentage of sperm with normal morphology(r=-0.07, P<0.01), and sperm head deformity rate(r=-0.03, P<0.01), while positively correlated with sperm DFI(r=0.255, P<0.01). Conclusion: The infertile male age affects the normal morphology and DNA integrity of sperm. As the age increases, the percentage of sperm with normal morphology descends while the sperm DFI augments, and the age is significantly correlated with the percentage of sperm with normal morphology and sperm DFI.
Objective: To analyze the relationship between infertile male age and sperm morphology, sperm DNA fragment index(DFI). Methods: The sperm morphological parameters of 7 067 infertile male patients from 2014 to 2018 and the sperm DFI of 1 808 from 2015 to 2017 were analyzed retrospectively.The patients were divided into 4 groups according to their age: ≤25 years old, 26-35 years old, 36-45 years old, and >45 years old. Comparison and correlation analysis among their sperm morphological parameters. DFI and age were conducted. Results: With the increase of age, the percentage of sperm with normal morphology and the rate of sperm head deformity decreased while the sperm DFI increased. The percentages of sperm with normal morphology in 4 groups were(6.14 ± 2.39)%,(6.12±2.48)%,(5.95±2.32)% and(5.48±2.04)%, respectively. There were significant differenc-es between the two groups except ≤25 years group and 26～35 years group, ≤25 and 36-45 years group. The rates of sperm head deformity in each group were(86.55 ± 5.07)%,(86.16 ± 5.08)%,(85.82± 5.27)% and(85.56± 5.19)%, respectively. There was no significant difference between ≤25 years group and 26-35 years group, 26-35 years group and >45 years group, 36-45 years group and >45 years group. The sperm DFI of each group was(10.44 ± 4.32)%,(12.26 ± 6.84)%,(14.01± 7.59)% and(20.08± 10.14)%, respectively. The infertile male age was significantly negatively correlated with percentage of sperm with normal morphology(r=-0.07, P<0.01), and sperm head deformity rate(r=-0.03, P<0.01), while positively correlated with sperm DFI(r=0.255, P<0.01). Conclusion: The infertile male age affects the normal morphology and DNA integrity of sperm. As the age increases, the percentage of sperm with normal morphology descends while the sperm DFI augments, and the age is significantly correlated with the percentage of sperm with normal morphology and sperm DFI.
引文
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[14]Champroux A,Torres-Carreira J,Gharagozloo P,et al.Mammalian sperm nuclear organization:resiliencies and vulnerabilities[J].Basic Clin Androl,2016,26:17.
[15]Noblanc A,Kocer A,Drevet JR.Recent knowledge concerning mammalian sperm chromatin organization and its potential weaknesses when facing oxidative challenge[J].Basic Clin Androl,2014,24:6.
[16]Muratori M,Marchiani S,Maggi M,et al.Origin and biological significance of DNA fragmentation in human spermatozoa[J].Front Biosci,2006,11:1 491-1 499.
[17]Gavriliouk D,Aitken RJ.Damage to sperm DNA mediated by reactive oxygen species:Its impact on human reproduction and the health trajectory of offspring[J].Adv Exp Med Biol,2015,868:23-47.
[18]Bejarano I,Rodríguez AB,Pariente JA.Apoptosis is a demanding selective tool during the development of fetal male germ cells[J].Front Cell Dev Biol,2018,6:65.
[19]Sakkas D,Seli E,Bizzaro D,et al.Abnormal spermatozoa in the ejaculate:abortive apoptosis and faulty nuclear remodelling during spermatogenesis[J].Reprod Biomed Online,2003,7(4):428-432.
[20]Das M,Al-Hathal N,San-Gabriel M,et al.High prevalence of isolated sperm DNA damage in infertile men with advanced paternal age Assist[J].J Assist Reprod Genet,2013,30(6):843-848.
[21]Hammiche F,Laven JS,Boxmeer JC,et al.Sperm quality decline among men below 60 years of age undergoing IVF or ICSI treatment[J].J Androl,2011,32(1):70-76.
[22]Rybar R,Kopecka V,Prinosilova P,et al.Male obesity and age in relationship to semen parameters and sperm chromatin integrity[J].Andrologia,2011,43(4):286-291.
[23]Singh NP,Muller CH,Berger RE,et al.Effects of age on DNA double-strand breaks and apoptosis in human sperm[J].Fertil Steril,2003,80(2):1 420-1 430.
[24]Omu AE,AI-Azemi MK,Kehinde EO,et al.Indications of the mechanisms involved in improved sperm parameters by zinc therapy[J].Med Princ Pract,2008,17(2):108-116.
[25]Aboutorabi R,Zamani S,Zarrin Y,et al.A survey on main semen parameters in natural pregnancy and intrauterine insemination:Are there any significant differences[J]?Am J Mens Health,2018,12(3):617-623.
[26]Tomlinson M,Lewis S,Morroll D,et al.Sperm quality and its relationship to natural and assisted conception:British Fertility Society guidelines for practice[J].Hum Fertil(Camb),2013,16(3):175-193.
[27]刘安娜.男性不育患者年龄对精子形态及精子DNA完整性的影响分析[J].中国优生与遗传杂志,2014,22(5):131,63.Liu AN.Analysis of effects of sterile patient ages on sperm morphology and sperm DNA integrity[J].Chinese Journal of Birth&Heredity,2014,22(5):131,63.
[28]侯开波,崔鑫,任权,等.年龄对男性精液参数及DNA完整性的影响[J].中国优生与遗传杂志,2015,23(9):116-117.Hou KB,Cui X,Ren Q,et al.Effects of advanced paternal age on standard sperm parameters and sperm DNA integrity[J].Chinese Journal of Birth&Heredity,2015,23(9):116-117.
[2]Kaarouch I,Bouamoud N,Madkour A,et al.Paternal age:Negative impact on sperm genome decays and IVF outcomes after 40 years[J].Mol Reprod Dev,2018,85(3):271-280.
[3]Agarwal A,Allamaneni SS.Sperm DNA damage assessment:a test whose time has come[J].Fertil Steril,2005,84(4):850-853.
[4]Barazani Y,Agarwal A,Sabanegh ES Jr.Functional sperm testing and the role of proteomics in the evaluation of male infertility[J].Urology,2014,84(2):255-261.
[5]Zidi-Jah I,Hajlaoui A,Mougou-Zerelli S,et al.Relationship between sperm aneuploidy,sperm DNA integrity,chromatin packaging,traditional semen parameters,and recurrent pregnancy loss[J].Fertil Steril,2016,105(1):58-64.
[6]Lopez G,Lafuente R,Checa MA,et al.Diagnostic value of sperm DNA fragmentation and sperm high-magnification for predicting outcome of assisted reproduction treatment[J].Asian J Androl,2013,15(6):790-794.
[7]Wright C,Milne S,Leeson H,et al.Sperm DNAdamage caused by oxidative stress:modifiable clinical,lifestyle and nutritional factors in male infertility[J].Reprod Biomed Online,2014,28(6):684-703.
[8]Soheila P,Parvin S,Tahereh R,et al.The etiologies of DNA abnormalities in male infertility:An assessment and review[J].Int J Reprod Biomed(Yazd),2017,15(6):331-344.
[9]Zini A,Bielecki R,Phang D,et al.Correlations between two markers of sperm DNA integrity,DNA denaturation and DNA fragmentation,in fertile and infertile men[J].Fertil Steril,2001,75:674-677.
[10]Zini A,Kamal K,Phang D,et al.Biologic variability of sperm DNA denaturation in infertile men[J].Urology,2001,58(2):258-261.
[11]Atig F,Kerkeni A,Saad A,et al.Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men[J].JAssist Reprod Genet,2017,34(3):373-381.
[12]符浩,宋汶柯,凌晓辉,等.精索静脉曲张不育患者氧化应激水平和精子DNA完整性及精液参数的相关性分析[J].中华男科学杂志,2016,22(6):530-533.Fu H,Song WK,Ling XH,et al.Correlation of oxidative stress with sperm DNA integrity and semen param-eters in infertile men with varicocele[J].National J Andrology,2016,22(6):530-533.
[13]Hasanzadeh KS,Farsi MM,Khafri S.Should we perform semen analysis,DNA fragmentation,and hypo-osmotic wwelling test together[J]?Int J Mol Cell Med,2016,5(4):246-254.
[14]Champroux A,Torres-Carreira J,Gharagozloo P,et al.Mammalian sperm nuclear organization:resiliencies and vulnerabilities[J].Basic Clin Androl,2016,26:17.
[15]Noblanc A,Kocer A,Drevet JR.Recent knowledge concerning mammalian sperm chromatin organization and its potential weaknesses when facing oxidative challenge[J].Basic Clin Androl,2014,24:6.
[16]Muratori M,Marchiani S,Maggi M,et al.Origin and biological significance of DNA fragmentation in human spermatozoa[J].Front Biosci,2006,11:1 491-1 499.
[17]Gavriliouk D,Aitken RJ.Damage to sperm DNA mediated by reactive oxygen species:Its impact on human reproduction and the health trajectory of offspring[J].Adv Exp Med Biol,2015,868:23-47.
[18]Bejarano I,Rodríguez AB,Pariente JA.Apoptosis is a demanding selective tool during the development of fetal male germ cells[J].Front Cell Dev Biol,2018,6:65.
[19]Sakkas D,Seli E,Bizzaro D,et al.Abnormal spermatozoa in the ejaculate:abortive apoptosis and faulty nuclear remodelling during spermatogenesis[J].Reprod Biomed Online,2003,7(4):428-432.
[20]Das M,Al-Hathal N,San-Gabriel M,et al.High prevalence of isolated sperm DNA damage in infertile men with advanced paternal age Assist[J].J Assist Reprod Genet,2013,30(6):843-848.
[21]Hammiche F,Laven JS,Boxmeer JC,et al.Sperm quality decline among men below 60 years of age undergoing IVF or ICSI treatment[J].J Androl,2011,32(1):70-76.
[22]Rybar R,Kopecka V,Prinosilova P,et al.Male obesity and age in relationship to semen parameters and sperm chromatin integrity[J].Andrologia,2011,43(4):286-291.
[23]Singh NP,Muller CH,Berger RE,et al.Effects of age on DNA double-strand breaks and apoptosis in human sperm[J].Fertil Steril,2003,80(2):1 420-1 430.
[24]Omu AE,AI-Azemi MK,Kehinde EO,et al.Indications of the mechanisms involved in improved sperm parameters by zinc therapy[J].Med Princ Pract,2008,17(2):108-116.
[25]Aboutorabi R,Zamani S,Zarrin Y,et al.A survey on main semen parameters in natural pregnancy and intrauterine insemination:Are there any significant differences[J]?Am J Mens Health,2018,12(3):617-623.
[26]Tomlinson M,Lewis S,Morroll D,et al.Sperm quality and its relationship to natural and assisted conception:British Fertility Society guidelines for practice[J].Hum Fertil(Camb),2013,16(3):175-193.
[27]刘安娜.男性不育患者年龄对精子形态及精子DNA完整性的影响分析[J].中国优生与遗传杂志,2014,22(5):131,63.Liu AN.Analysis of effects of sterile patient ages on sperm morphology and sperm DNA integrity[J].Chinese Journal of Birth&Heredity,2014,22(5):131,63.
[28]侯开波,崔鑫,任权,等.年龄对男性精液参数及DNA完整性的影响[J].中国优生与遗传杂志,2015,23(9):116-117.Hou KB,Cui X,Ren Q,et al.Effects of advanced paternal age on standard sperm parameters and sperm DNA integrity[J].Chinese Journal of Birth&Heredity,2015,23(9):116-117.