林丹对大鼠精子生成的影响
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摘要
目的探讨林丹对雄性大鼠精子生成的影响。方法 40只3～4周龄、体质量80～100g的雄性SD大鼠随机分成5组:空白对照组(玉米油)、阳性对照组(雌二醇)和林丹低、中、高剂量组(7mg·kg-1、13mg·kg-1、25mg·kg-1),每组8只。每组每天灌胃1次,连续灌胃8周后,对大鼠体质量、脏器系数、精子密度和存活率进行测定。结果各实验组体质量增长量与空白对照组、阳性对照组相比差异无统计学意义(P>0.05)。阳性对照组各脏器系数与空白对照组相比差异均无统计学意义(P均>0.05)。低、中、高剂量组肝脏系数均低于阳性对照组(P均<0.05),与空白对照组相比差异无统计学意义(P>0.05);睾丸、附睾系数各实验组与空白对照组、阳性对照组差异均无统计学意义(P均>0.05)。阳性对照组精子密度、精子存活率低于空白对照组(P<0.05)。各实验组精子密度和存活率均低于空白对照组,高于阳性对照组(P均<0.05)。结论林丹对雄性大鼠精子的生成具有抑制作用,在本实验条件下其抑制效果较雌二醇轻。
Objective To investigate the effect of lindane on spermatogenesis of male rats. Methods Forty male SD rats aged 3 to 4 weeks and weighing 80 g to 100 g were randomly divided into 5 groups,8 in each group. They were the blank control group(corn oil),the positive control group(estradiol)and the 7 mg·kg-1,13 mg·kg-1,25 mg·kg-1 lindane exposure group,once a day. After 8 weeks of continuous intragastric administration,the rat body weight, organ coefficient,sperm density and survival rate were measured.Results There was no significant difference in weight gain between the experimental groups and the blank control group and the positive control group(P>0.05). There was no significant difference in the organ coefficient between the positive control group and the blank control group(P>0.05). The liver coefficient of the low,middle and high dose groups was lower than that of the positive control group(P<0.05),and there was no significant difference compared with the blank control group(P>0.05);the testis and epididymis coefficient of the experimental group had no significant difference compared with the blank control group and the positive control group(P>0.05). The sperm density and survival rate of positive control group were lower than those of blank control group(P<0.05). The sperm density and survival rate of each experimental group were lower than those of the blank control group(P<0.05),but higher than those of the positive control group(P<0.05). Conclusion Lindane has an inhibitory effect on the production of sperm in male rats,and its inhibitory effect is lighter than that of estradiol under the conditions of this experiment.
Objective To investigate the effect of lindane on spermatogenesis of male rats. Methods Forty male SD rats aged 3 to 4 weeks and weighing 80 g to 100 g were randomly divided into 5 groups,8 in each group. They were the blank control group(corn oil),the positive control group(estradiol)and the 7 mg·kg-1,13 mg·kg-1,25 mg·kg-1 lindane exposure group,once a day. After 8 weeks of continuous intragastric administration,the rat body weight, organ coefficient,sperm density and survival rate were measured.Results There was no significant difference in weight gain between the experimental groups and the blank control group and the positive control group(P>0.05). There was no significant difference in the organ coefficient between the positive control group and the blank control group(P>0.05). The liver coefficient of the low,middle and high dose groups was lower than that of the positive control group(P<0.05),and there was no significant difference compared with the blank control group(P>0.05);the testis and epididymis coefficient of the experimental group had no significant difference compared with the blank control group and the positive control group(P>0.05). The sperm density and survival rate of positive control group were lower than those of blank control group(P<0.05). The sperm density and survival rate of each experimental group were lower than those of the blank control group(P<0.05),but higher than those of the positive control group(P<0.05). Conclusion Lindane has an inhibitory effect on the production of sperm in male rats,and its inhibitory effect is lighter than that of estradiol under the conditions of this experiment.
引文
[1]朱永平,王敏,黄幸.六六六和林丹的大鼠致畸研究[J].癌变.畸变.突变,1990,2(4):19-23,89.
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[4] Di Consiglio E,De Angelis G,Traina ME,et al. Effect of lindane on CYP-mediated steroid hormone metabolism in male mice following in utero exposure[J]. J Appl Toxicol,2009,29(8):648-655.
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[6]刘传志,张莉,刘振瑶,等.雌二醇激素对大鼠生理功能的影响[J].长理工大学学报(自然科学版),2017,40(5):125-127.
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[9]李洁斐,李卫华,杨健,等. 17-α炔雌醇对斑马鱼的卵黄蛋白原及性腺发育的影响[J].毒理学杂志,2006,20(1):9-13.
[10] Bell AM. An endocrine disrupter increases growth and risky behavior in threespined stickleback(Gasterosteus aculeatus)[J]. Horm Behav,2004,45(2):108-114.
[11]姚建仁,劭向东,赵静,等.林丹的作用机制[J].国外农业环境保护,1993(1):10-12.
[12]袁本利.药物安全评价中脏器系数的意义及不足[J].中国新药杂志,2003,12(11):960-963.
[13] Sumida K,Saito K,Oeda K,et al. A comparative study of gene expression profiles in rat liver after administration of alpha-hexachlorocyclohexane and lindane[J]. J Toxicol Sci,2007,32(3):261-288.
[14]王士然,王蕾,马兆雷.环境内分泌干扰物DBP与BPA、联合染毒对雄性大鼠生殖功能的影响[J].预防医学论坛,2013,19(10):721-723.
[15]张仁义.孕期暴露壬基酚和雌二醇对F1雄性大鼠生殖系统的联合作用[D].遵义:遵义医学院,2009.
[16]刘传志,张莉,刘振瑶,等.雌二醇激素对大鼠生理功能的影响[J].长春理工大学学报(自然科学版),2017,40(5):125-127.
[17] Michán S,Li Y,Chou MM,et al. SIRT1 is essential for normal cognitive function and synaptic plasticity[J]. J Neurosci,2010,30(29):9695-9707.
[18]李雪骥.壬基酚对SD大鼠胰腺结构及胰岛素分泌功能影响的体内外研究[D].苏州:苏州大学,2017.
[19] Wang ZX,Wreford NG,De Kretser DM. Determination of Sertoli cell numbers in the developing rat testis by stereological methods[J]. Int J Androl,1989,12(1):58-64.
[20] Gong Y,Han XD. Nonylphenol-induced oxidative stress and cytotoxicity in testicular Sertoli cells[J].Reprod Toxicol,2006,22(4):623-630.
[2]任仁,黄俊.哪些物质属于内分泌干扰物(EDCs)[J].安全与环境工程,2004,11(3):7-10.
[3]吴迪,单正军,韩志华,等.林丹短期暴露对斑马鱼生长发育和繁殖的影响[J].生态与农村环境学报,2011,27(1):49-53.
[4] Di Consiglio E,De Angelis G,Traina ME,et al. Effect of lindane on CYP-mediated steroid hormone metabolism in male mice following in utero exposure[J]. J Appl Toxicol,2009,29(8):648-655.
[5]朱永平,王敏,黄幸.工业品六氯环己烷和林丹对大鼠的生殖毒性[J].中国药理学与毒理学杂志,1990,4(4):292.
[6]刘传志,张莉,刘振瑶,等.雌二醇激素对大鼠生理功能的影响[J].长理工大学学报(自然科学版),2017,40(5):125-127.
[7]王心如.毒理学基础[M].北京:人民卫生出版社,2003.
[8] Guo TL,Germolec DR,Musgrove DL,et al. Myelotoxicity in genistein-,nonylphenol-,methoxychlor-,vinclozolin-or ethinyl estradiol-exposed F1 generations of Sprague-Dawley rats following developmental and adult exposures[J]. Toxicology,2005,211(3):207-219.
[9]李洁斐,李卫华,杨健,等. 17-α炔雌醇对斑马鱼的卵黄蛋白原及性腺发育的影响[J].毒理学杂志,2006,20(1):9-13.
[10] Bell AM. An endocrine disrupter increases growth and risky behavior in threespined stickleback(Gasterosteus aculeatus)[J]. Horm Behav,2004,45(2):108-114.
[11]姚建仁,劭向东,赵静,等.林丹的作用机制[J].国外农业环境保护,1993(1):10-12.
[12]袁本利.药物安全评价中脏器系数的意义及不足[J].中国新药杂志,2003,12(11):960-963.
[13] Sumida K,Saito K,Oeda K,et al. A comparative study of gene expression profiles in rat liver after administration of alpha-hexachlorocyclohexane and lindane[J]. J Toxicol Sci,2007,32(3):261-288.
[14]王士然,王蕾,马兆雷.环境内分泌干扰物DBP与BPA、联合染毒对雄性大鼠生殖功能的影响[J].预防医学论坛,2013,19(10):721-723.
[15]张仁义.孕期暴露壬基酚和雌二醇对F1雄性大鼠生殖系统的联合作用[D].遵义:遵义医学院,2009.
[16]刘传志,张莉,刘振瑶,等.雌二醇激素对大鼠生理功能的影响[J].长春理工大学学报(自然科学版),2017,40(5):125-127.
[17] Michán S,Li Y,Chou MM,et al. SIRT1 is essential for normal cognitive function and synaptic plasticity[J]. J Neurosci,2010,30(29):9695-9707.
[18]李雪骥.壬基酚对SD大鼠胰腺结构及胰岛素分泌功能影响的体内外研究[D].苏州:苏州大学,2017.
[19] Wang ZX,Wreford NG,De Kretser DM. Determination of Sertoli cell numbers in the developing rat testis by stereological methods[J]. Int J Androl,1989,12(1):58-64.
[20] Gong Y,Han XD. Nonylphenol-induced oxidative stress and cytotoxicity in testicular Sertoli cells[J].Reprod Toxicol,2006,22(4):623-630.