血清25-羟维生素D与精液质量相关性研究
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摘要
目的探讨血清25-羟维生素D(25-OHD)水平与精液参数之间的关系。方法测定2 023例行体外受精-胚胎移植助孕的健康男性血清25-OHD水平,按其水平分为5组:A组109例,25-OHD <50 nmol/L; B组485例,50 nmol/L <25-OHD≤70 nmol/L; C组664例,70 nmol/L <25-OHD≤90 nmol/L; D组434例,90 nmol/L <25-OHD≤110 nmol/L; E组331例,25-OHD≥110 nmol/L。禁欲3～7 d后手淫法获取精液标本,应用精子质量分析仪(CASA)检测精子,按《WHO人类精液检查与处理实验手册》第5版要求检测精子形态。比较各组年龄、精子浓度、前向运动精子总数、存活率、精子正常形态百分率。结果各组年龄、精子浓度、前向运动精子总数、存活率、精子正常形态百分率差异无统计学意义(P均> 0. 05)。结论在柳州地区,血清25-OHD水平可能与精液质量无关。
Objective It is to investigate the relationship between serum 25-hydroxyvitamin D(25-OHD) level and semen parameters. Methods The level of serum 25-OHD were measured in 2023 healthy men undergoing in vitro fertilizationembryo transfer,the subjects were divided into 5 groups according to the level of 25-OHD,group A(n = 109),25-OHD <50 nmol/L; group B(n = 485),50 nmol/L < 25-OHD≤70 nmol/L; group C(n = 664),70 nmol/L < 25-OHD≤90nmol/L; group D(n = 434),90 nmol/L < 25-OHD≤110 nmol/L; group E(n = 331),25-OHD≥110 nmol/L. After 3-7days of abstinence,the semen specimen was obtained by masturbation method,and the sperm was detected by the sperm quality analyzer(CASA). The sperm morphology was detected according to the fifth edition of WHO Manual for Human Semen Examination and Treatment. The age,sperm concentration,total number of forward-moving sperm,survival rate,and percentage of normal sperm morphology were compared in each group. Results There were no significant difference in age,sperm concentration,total number of forward-moving sperm,survival rate,and percentage of normal sperm morphology among each group. Conclusion Serum 25-OHD level may not be related to semen quality in Liuzhou area.
Objective It is to investigate the relationship between serum 25-hydroxyvitamin D(25-OHD) level and semen parameters. Methods The level of serum 25-OHD were measured in 2023 healthy men undergoing in vitro fertilizationembryo transfer,the subjects were divided into 5 groups according to the level of 25-OHD,group A(n = 109),25-OHD <50 nmol/L; group B(n = 485),50 nmol/L < 25-OHD≤70 nmol/L; group C(n = 664),70 nmol/L < 25-OHD≤90nmol/L; group D(n = 434),90 nmol/L < 25-OHD≤110 nmol/L; group E(n = 331),25-OHD≥110 nmol/L. After 3-7days of abstinence,the semen specimen was obtained by masturbation method,and the sperm was detected by the sperm quality analyzer(CASA). The sperm morphology was detected according to the fifth edition of WHO Manual for Human Semen Examination and Treatment. The age,sperm concentration,total number of forward-moving sperm,survival rate,and percentage of normal sperm morphology were compared in each group. Results There were no significant difference in age,sperm concentration,total number of forward-moving sperm,survival rate,and percentage of normal sperm morphology among each group. Conclusion Serum 25-OHD level may not be related to semen quality in Liuzhou area.
引文
[1] Blomberg Jensen M,Nielsen JE,Jorgensen A,et al. Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract[J]. Hum Reprod,2010,25(5):1303-1311
[2] Sood S,Reghunandanna R,Reghunandanan V,et al.Effect of vitamin D repletion on testicular function in vitamin D-deficient rats[J]. Ann Nutr Metab,1995,39(2):95-98
[3] Lerchbaum E,Obermayer-Pietsch B. Vitamin D and fertility:a systematic review[J]. Eur J Endocrinol,2012,166(5):765-778
[4] Wang LL,Wang HY,Wen HK,et al. Vitamin D status among infants,children,and adolescents in southeastern China[J]. J Zhejiang Univ Sci B,2016,17(7):545-552
[5] Zhao X,Fang R,Yu R,et al. Maternal Vitamin D Status in the Late Second Trimester and the Risk of Severe Preeclampsia in Southeastern China[J]. Nutrients,2017,14,9(2):138
[6] Blomberg Jensen M. Vitamin D and male reproduction[J]. Nat Re Endocrinol,2014,10(3):175-186
[7] Dabrowski FA,Gezechocinska B,Wielgos M. The role of vitamin D in reproductive health-a Trojan Horse or the Golden Fleece[J]. Nutrients,2015,29,7(6):4139-4153
[8] Blomberg Jensen M,Nielsen JE,Jorgensen A,et al. Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract[J]. Hum Reprod,2010,25(5):1303-1311
[9] Aquila S,Guido C,Middea E,et al. Human male gamete endocrinology:1alpha,25-dihydroxyvitamin D3(1,25(OH)2D3)regulates different aspects of human sperm biology and metabolism[J]. Reprod Biol Endocrinol,2009,7(1):140-153
[10] Blomberg Jensen M. Vitamin D metabolism,sex hormones,and male reproductive function[J]. Reproduction,2012,144(2):135-152
[11] Blomberg Jensen M,Gerner Laeaetz J,Andersson AM,et al. Vitamin D deficiency and low ionized calcium are linked with semen quality and sex steroid levels in infertile men[J]. Hum Reprod,2016,31(8):1875-1885
[12]邓小林,黎衍敏,黄建荣. 25-羟化维生素D和精液常规参数的相关性分析[J].中国男科学杂志,2014,18(12):29-32
[13]贾新转,魏兰.血清25-OHD水平与精液参数的关系[J].天津医药,2015,43(7):762-764
[14]Ramlau-Hansen CH,Moeller UK,Bonde JP,et al. Are serum levels of vitamin D associated with semen quality Results from a cross-sectional study in young healthy men[J]. Fertil Steril,2011,95(3):1000-1004
[15]Pacis MM,Fortin CN,Zarek SM. Vitamin D and assisted reproduction:should vitamin D be routinely screened and repleted prior to ART A systematic review[J]. J Assist Reprod Genet,2015,32(3):323-335
[16]Nandi A,Sinha N,Ong E,et al. Is there a role for Vitamin D in human reproduction[J]. Horm Mol Biol Clin Investig,2016,25(1):15-28
[17]王丽萍,黄金.孕期维生素D水平与不良妊娠结局相关性研究进展[J].实用医学杂志. 2015,33(10):3446-3448
[18]Abada L,Gaskins AJ,Chiu YH,et al. Serum 25-hydroxyvitamin D concentrations and treatment outcomes of women undergoing assisted reproduction[J]. Am J Clin Nutr,2016,104(3):729-735
[19]Vanni VS,Vigano P,Somigliana E. Vitamin D and assisted reproduction technologies:current concepts[J]. Reprod Biol Endocrinol,2014,31(12):47
[2] Sood S,Reghunandanna R,Reghunandanan V,et al.Effect of vitamin D repletion on testicular function in vitamin D-deficient rats[J]. Ann Nutr Metab,1995,39(2):95-98
[3] Lerchbaum E,Obermayer-Pietsch B. Vitamin D and fertility:a systematic review[J]. Eur J Endocrinol,2012,166(5):765-778
[4] Wang LL,Wang HY,Wen HK,et al. Vitamin D status among infants,children,and adolescents in southeastern China[J]. J Zhejiang Univ Sci B,2016,17(7):545-552
[5] Zhao X,Fang R,Yu R,et al. Maternal Vitamin D Status in the Late Second Trimester and the Risk of Severe Preeclampsia in Southeastern China[J]. Nutrients,2017,14,9(2):138
[6] Blomberg Jensen M. Vitamin D and male reproduction[J]. Nat Re Endocrinol,2014,10(3):175-186
[7] Dabrowski FA,Gezechocinska B,Wielgos M. The role of vitamin D in reproductive health-a Trojan Horse or the Golden Fleece[J]. Nutrients,2015,29,7(6):4139-4153
[8] Blomberg Jensen M,Nielsen JE,Jorgensen A,et al. Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract[J]. Hum Reprod,2010,25(5):1303-1311
[9] Aquila S,Guido C,Middea E,et al. Human male gamete endocrinology:1alpha,25-dihydroxyvitamin D3(1,25(OH)2D3)regulates different aspects of human sperm biology and metabolism[J]. Reprod Biol Endocrinol,2009,7(1):140-153
[10] Blomberg Jensen M. Vitamin D metabolism,sex hormones,and male reproductive function[J]. Reproduction,2012,144(2):135-152
[11] Blomberg Jensen M,Gerner Laeaetz J,Andersson AM,et al. Vitamin D deficiency and low ionized calcium are linked with semen quality and sex steroid levels in infertile men[J]. Hum Reprod,2016,31(8):1875-1885
[12]邓小林,黎衍敏,黄建荣. 25-羟化维生素D和精液常规参数的相关性分析[J].中国男科学杂志,2014,18(12):29-32
[13]贾新转,魏兰.血清25-OHD水平与精液参数的关系[J].天津医药,2015,43(7):762-764
[14]Ramlau-Hansen CH,Moeller UK,Bonde JP,et al. Are serum levels of vitamin D associated with semen quality Results from a cross-sectional study in young healthy men[J]. Fertil Steril,2011,95(3):1000-1004
[15]Pacis MM,Fortin CN,Zarek SM. Vitamin D and assisted reproduction:should vitamin D be routinely screened and repleted prior to ART A systematic review[J]. J Assist Reprod Genet,2015,32(3):323-335
[16]Nandi A,Sinha N,Ong E,et al. Is there a role for Vitamin D in human reproduction[J]. Horm Mol Biol Clin Investig,2016,25(1):15-28
[17]王丽萍,黄金.孕期维生素D水平与不良妊娠结局相关性研究进展[J].实用医学杂志. 2015,33(10):3446-3448
[18]Abada L,Gaskins AJ,Chiu YH,et al. Serum 25-hydroxyvitamin D concentrations and treatment outcomes of women undergoing assisted reproduction[J]. Am J Clin Nutr,2016,104(3):729-735
[19]Vanni VS,Vigano P,Somigliana E. Vitamin D and assisted reproduction technologies:current concepts[J]. Reprod Biol Endocrinol,2014,31(12):47