基于一测多评法的丹参酮提取物质量控制
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摘要
目的:建立丹参酮提取物中二氢丹参酮I、隐丹参酮、丹参酮I和丹参酮IIA 4种丹参酮类成分的一测多评法,探讨一测多评法在丹参酮提取物质量控制中的应用。方法:采用高效液相色谱法,以丹参酮IIA为内标物,计算二氢丹参酮I、隐丹参酮和丹参酮I相对于丹参酮IIA的相对校正因子和相对保留时间,利用相对校正因子计算丹参酮提取物中4种丹参酮类成分的含量,实现一测多评;同时采用外标法测定4种丹参酮类成分的含量,并比较二者差异,评价一测多评法在丹参酮提取物质量控制应用中的准确性和科学性。结果:二氢丹参酮I、隐丹参酮和丹参酮I相对于丹参酮IIA的相对校正因子分别为1.55、1.12和1.28;各成分的相对校正因子和相对保留时间在不同实验条件下的系统耐用性良好;一测多评法计算结果与外标法测定结果无显著差异。结论:以丹参酮IIA为内标物,建立的丹参酮提取物中4种丹参酮类成分的一测多评方法简便、准确、可靠,可用于丹参酮提取物的多成分质量控制。
Objective:To develop a single standard for determination of multiple components method(SSDMC)for the quantitative analysis of four active tanshinones(dihydrotanshinone I,cryptotanshinone,tanshinone I and tanshinones IIA)in tanshinone extract,and discuss the application of SSDMC method in quality control of tanshinone extract.Methods:The relative conversion factors(RFs)and relative retention time(RRT)of dihydrotanshinone I,cryptotanshinone,tanshinone I were calculated using tanshinone IIA as the reference standard,and the contents of the four tanshinones in tanshinone extract were determined by SSDMC and external standard method(ESM).Results:The RFs of dihydrotanshinone I,cryptotanshinone,tanshinone I were 1.55,1.12 and 1.28,respectively,and repeatability was good in different experimental conditions.There was no significant difference between the quantitative results of the SSDMC and ESM methods.Conclusion:The established SSDMC method was simple,accurate and reliable and could be a well-acceptable approach for quality control of tanshinone extract.
Objective:To develop a single standard for determination of multiple components method(SSDMC)for the quantitative analysis of four active tanshinones(dihydrotanshinone I,cryptotanshinone,tanshinone I and tanshinones IIA)in tanshinone extract,and discuss the application of SSDMC method in quality control of tanshinone extract.Methods:The relative conversion factors(RFs)and relative retention time(RRT)of dihydrotanshinone I,cryptotanshinone,tanshinone I were calculated using tanshinone IIA as the reference standard,and the contents of the four tanshinones in tanshinone extract were determined by SSDMC and external standard method(ESM).Results:The RFs of dihydrotanshinone I,cryptotanshinone,tanshinone I were 1.55,1.12 and 1.28,respectively,and repeatability was good in different experimental conditions.There was no significant difference between the quantitative results of the SSDMC and ESM methods.Conclusion:The established SSDMC method was simple,accurate and reliable and could be a well-acceptable approach for quality control of tanshinone extract.
引文
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[2] ZHOU L,ZHONG Z,CHOW M S.Danshen:An overview of its chemistry,pharmacology,pharmacokinetics,and clinical use[J].J Clin Pharmacol,2006,45(12):1345-1359.
[3] 桑梦如,黄楚璇,吴啟南,等.不同规格商品丹参质量比较研究[J].中国现代中药,2017,19(3):430-435.
[4] 戴新新,宿树兰,郭盛,等.丹参酮类成分的生物活性与应用开发研究进展[J].中草药,2017,48(7):1442-1448.
[5] 刘慧颖,姜长涛,冯娟,等.丹参酮类化合物研究进展[J].中国药理学通报,2016,32(12):1643-1647.
[6] LAM F F,YEUNG J H,CHEUNF J H,et al.Pharmacological evidence for calcium channel inhibition by danshen(Salvia miltiorrhiza)on rat isolated femoral artery[J].J Cardiovasc Pharmacol,2006,47(47):139-145.
[7] WANG X,MORRIS-NATSCHKE S L,LEE K H.New developments in the chemistry and biology of the bioactive constituents of Tanshen[J].Med Res Rev,2007,27(1):133-148.
[8] 汤瑶,李佐刚,王秀文,等.超高效液相色谱法同时测定丹参酮提取物中隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA[J].药物分析杂志,2014,34(5):813-818.
[9] 张源源,田友清.总丹参酮超声提取及其主要成分的HPLC法测定[J].安徽农业科学,2011,39(4):2083-2084.
[10] LI S P,ZHAO J,YANG B.Strategies for quality control of Chinese medicines[J].J Pharm Biomed Anal,2011,55(4):802-809.
[11] YONG J,DAVID B,TU P,et al.Recent analytical approaches in quality control of traditional Chinese medicines-A review[J].Anal Chim Acta,2010,657(1):9-18.
[12] 田宇柔,麻景梅,王鑫国,等.一测多评法同时测定山茱萸配方颗粒中5种成分[J].中成药,2017,39(9):1845-1849.
[13] GAO W,WANG R,LI D,et al.Comparison of five Lonicera flowers by simultaneous determination of multi-components with single reference standard method and principal component analysis[J].J Pharm Biomed Anal,2016,117(1),345-351.
[14] HOU J J,WU W Y,DA J,et al.Ruggedness and robustness of conversion factors in method of simultaneous determination of multi-components with single reference standard[J].J Chromatogr A,2011,1218(33),5618-5627.
[15] 李筱筱,武雪玲,贾世亮,等.丹参酮ⅡA药理作用的研究进展[J].生物学杂志,2016,33(6):91-94.