晚期糖基化终末产物受体及胞内信号分子在肺腺癌细胞中的表达及功能
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摘要
目的探讨在肺腺癌细胞株A549中,晚期糖基化终末产物受体(RAGE)及其胞内信号分子DIAPH1的表达及对细胞迁移及凋亡能力的影响。方法 (1)以肺腺癌细胞株A549及正常人支气管上皮细胞株BEAS-2B为研究对象,利用qRT-PCR、Western blot检测RAGE及DIAPH1在二者中的表达;(2)以10、100μg/ml RAGE配体CML-AGE及1、10、100μg/ml RAGE配体S100B处理A549细胞,划痕实验检测其对细胞迁移能力的影响;(3)以25、50、100μg/ml RAGE配体CML-AGE处理A549细胞,qRT-PCR法检测凋亡相关基因BCL-2和BAX的表达情况。结果 (1) qRT-PCR、Western blot检测结果显示A549细胞中RAGE及DIAPH1表达较BEAS-2B细胞明显下调(P <0. 001);(2) 10、100μg/ml RAGE配体CML-AGE及1、10、100μg/ml RAGE配体S100B处理对A549细胞的迁移能力皆有明显抑制作用,且作用呈浓度依赖性(P <0. 01),差异有统计学意义;(3)以25、50、100μg/ml RAGE配体CML-AGE处理A549细胞后,抗凋亡基因BCL-2表达下调,促凋亡基因BAX表达上调,其作用呈浓度依赖性(P <0. 05),差异有统计学意义。结论 RAGE及DIAPH1在肺腺癌细胞株A549中的表达低于正常人支气管上皮细胞BEAS-2B。RAGE配体在一定程度上可以抑制A549细胞的迁移,促进其凋亡,有望成为肺腺癌治疗新的靶点。
Objective To explore the expression of the receptor for advanced glycosylation end products( RAGE)and its intracellular signaling molecules DIAPH1 in lung adenocarcinoma A549 cells and the effect of RAGE ligands on cell migration and apoptosis. Methods The expressions of RAGE and DIAPH1 in lung adenocarcinoma A549 cells and human bronchial epithelial cells BEAS-2 B were tested by qRT-PCR and Western blot. A549 cells was treated with 10,100 μg/ml RAGE ligands CML-AGE and 1,10,100 μg/ml S100 B,and wound healing test was used to identify the effect of migration ability. A549 cells was treated with 25,50,100 μg/ml RAGE ligands CMLAGE,the gene expression of BCL-2 and BAX were tested by using qRT-PCR. Results The results of qRT-PCR and Western blot showed,compared with human bronchial epithelium cells BEAS-2 B,the expression of RAGE and DIAPH1 were both significantly down-regulated in lung adenocarcinoma A549 cells( P < 0. 001). After treated with 10,100 μg/ml RAGE ligands CML-AGE and 1,10,100 μg/ml S100 B,both groups showed the ligands inhibit lung adenocarcinoma A549 cells migration in concentration-depend manners( P < 0. 01). After treated with 25,50,100 μg/ml RAGE ligands CML-AGE,the expression of anti-apoptotic gene BCL-2 was down-regulated and proapoptotic gene BAX was upregulated in the experimental group in concentration-depend manners( P < 0. 05),the difference was significant. Conclusion The expression levels of RAGE and DIAPH1 in lung adenocarcinoma A549 cells are both significantly lower than human bronchial epithelium cells BEAS-2 B. RAGE ligands can inhibit cells migration and promote cell apoptosis in lung adenocarcinoma A549 cells and may provide a new target for the therapy of lung adenocarcinoma cells.
Objective To explore the expression of the receptor for advanced glycosylation end products( RAGE)and its intracellular signaling molecules DIAPH1 in lung adenocarcinoma A549 cells and the effect of RAGE ligands on cell migration and apoptosis. Methods The expressions of RAGE and DIAPH1 in lung adenocarcinoma A549 cells and human bronchial epithelial cells BEAS-2 B were tested by qRT-PCR and Western blot. A549 cells was treated with 10,100 μg/ml RAGE ligands CML-AGE and 1,10,100 μg/ml S100 B,and wound healing test was used to identify the effect of migration ability. A549 cells was treated with 25,50,100 μg/ml RAGE ligands CMLAGE,the gene expression of BCL-2 and BAX were tested by using qRT-PCR. Results The results of qRT-PCR and Western blot showed,compared with human bronchial epithelium cells BEAS-2 B,the expression of RAGE and DIAPH1 were both significantly down-regulated in lung adenocarcinoma A549 cells( P < 0. 001). After treated with 10,100 μg/ml RAGE ligands CML-AGE and 1,10,100 μg/ml S100 B,both groups showed the ligands inhibit lung adenocarcinoma A549 cells migration in concentration-depend manners( P < 0. 01). After treated with 25,50,100 μg/ml RAGE ligands CML-AGE,the expression of anti-apoptotic gene BCL-2 was down-regulated and proapoptotic gene BAX was upregulated in the experimental group in concentration-depend manners( P < 0. 05),the difference was significant. Conclusion The expression levels of RAGE and DIAPH1 in lung adenocarcinoma A549 cells are both significantly lower than human bronchial epithelium cells BEAS-2 B. RAGE ligands can inhibit cells migration and promote cell apoptosis in lung adenocarcinoma A549 cells and may provide a new target for the therapy of lung adenocarcinoma cells.
引文
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[13] Lizárraga F,Poincloux R,Romao M,et al. Diaphanous-related formins are required for invadopodia formation and invasion of breast tumor cells[J]. Cancer Res,2009,69(7):2792-2800.
[14] Danial N N. BCL-2 family proteins:critical checkpoints of apoptotic cell death[J]. Clin Cancer Res,2007,13(24):7254-63.
[15] Chiu T L,Su C C. Curcumin inhibits proliferation and migration by increasing the Bax to Bcl-2 ratio and decreasing NF-κBp65 expression in breast cancer MDA-MB-231 cells[J]. Int J Mol Med,2009,23(4):469-75.
[2] Tanji N,Markowitz G S,Fu C,et al. Expression of advanced glycation end products and their cellular receptor RAGE in diabetic nephropathy and nondiabetic renal disease[J]. J Am Soc Nephrol,2000,11(9):1656-66.
[3] Falcone C,Emanuele E,D'Angelo A,et al. Plasma levels of soluble receptor for advanced glycation end products and coronary artery disease in nondiabetic men[J]. Arterioscler Thromb Vasc Biol,2005,25(5):1032-37.
[4] Emanuele E,D'Angelo A,Tomaino C,et al. Circulating levels of soluble receptor for advanced glycation end products in Alzheimer disease and vascular dementia[J]. Arch Neurol,2005,62(11):1734-36.
[5] Riehl A,Németh J,Angel P,et al. The receptor RAGE:bridging inflammation cancer[J]. Cell Commun Signal,2009,7:12.
[6] Manigrasso M B,Pan J,Rai V,et al. Small molecule inhibition of ligand-stimulated RAGE-DIAPH1 signal transduction[J]. Sci Rep,2016,6:22450.
[7] Tanji M,Ishizaki T,Ebrahimi S,et al. m Dia1 targets v-Src to the cell periphery and facilitates cell transformation,tumorigenesis,and invasion[J]. Mol Cell Biol,2010,30(19):4604-15.
[8] Jemal A,Siegel R,Xu J,et al. Cancer Statistics,2010[J]. CA Cancer J Clin,2010,60(5):277-300.
[9] Bartling B,Hofmann H S,Weigle B,et al. Down-regulation of the receptor for advanced glycation end-products(RAGE)supports non-small cell lung carcinoma[J]. Carcinogenesis,2005,26(2):293-301.
[10] Schraml P,Bendik I,Ludwig C U. Differential messenger RNA and protein expression of the receptor for advanced glycosylated end products in normal lung and non-small cell lung carcinoma[J]. Cancer Res,1997,57(17):3669-71.
[11] Lin Y N,Izbicki J R,K9nig A,et al. Expression of DIAPH1 is up-regulated in colorectal cancer and its down-regulation strongly reduces the metastatic capacity of colon carcinoma cells[J]. Int J Cancer,2014,134(7):1571-82.
[12] Kim D,Jung J,You E,et al. m Dia1 regulates breast cancer invasion by controlling membrane type 1-matrix metalloproteinase localization[J]. Oncotarget,2016,7(14):17829-43.
[13] Lizárraga F,Poincloux R,Romao M,et al. Diaphanous-related formins are required for invadopodia formation and invasion of breast tumor cells[J]. Cancer Res,2009,69(7):2792-2800.
[14] Danial N N. BCL-2 family proteins:critical checkpoints of apoptotic cell death[J]. Clin Cancer Res,2007,13(24):7254-63.
[15] Chiu T L,Su C C. Curcumin inhibits proliferation and migration by increasing the Bax to Bcl-2 ratio and decreasing NF-κBp65 expression in breast cancer MDA-MB-231 cells[J]. Int J Mol Med,2009,23(4):469-75.