双丹明目胶囊对糖尿病大鼠模型视网膜VEGF家族表达的影响
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摘要
目的:观察双丹明目胶囊对糖尿病大鼠模型视网膜VEGF家族因子VEGF-a、VEGF-b、VEGF-c蛋白表达的影响。方法:将40只雄性SD大鼠,采用随机数字法分为A空白组、B模型组、C双丹明目组、D阳性对照组4组,每组10只20眼。将B、C、D三组实验大鼠采用STZ 50mg/kg的剂量一次性大鼠尾静脉注射法建立糖尿病视网膜病变大鼠模型,造模后1wk开始连续灌胃用药,灌胃后4wk,处死动物,免疫组化法检测视网膜组织中VEGF-a、VEGF-b、VEGF-c的表达。结果:成模后用药第4wk,模型组、双丹明目组、阳性对照组视网膜中VEGF-a、VEGF-b、VEGF-c蛋白表达平均灰度值均低于正常组,平均光密度均高于正常组,其中模型组与正常组比较,差异均具有统计学意义(P<0. 01);双丹明目组、阳性对照组VEGF-a、VEGF-b、VEGF-c表达的平均灰度值均高于模型组(P<0. 05),平均光密度值均低于模型组(P<0. 01)。结论:双丹明目胶囊能明显降低VEGF家族中VEGF-a、VEGF-b、VEGF-c在糖尿病模型大鼠视网膜中的表达,对其视网膜有一定的保护作用。
AIM: To study the effects of Shuangdan Mingmu capsule on the expressions of vascular endothelial growth factor-a( VEGF-a),VEGF-b,VEGF-c in the retina of a diabetic rat model. METHODS: Forty male SD rats were divided into Group A( normal group),Group B( model group),Group C( Shuangdan Mingmu group) and Group D( positive control group) 10 rats( 20 eyes) in each group.A rat model of diabetic retinopathy was established by one-time tail vein injection with STZ( 50 mg/kg). After modeling for 1 wk, the rats were given medicine by gavage. After gavage for 4 wk rats were sacrificed,and the expressions of VEGF-a,VEGF-b,VEGF-c in the retina tissues were detected by immunohistochemical method. RESULTS: After gavage for 4 wk the average gray values of VEGF-a,VEGF-b and VEGF-c protein in the retina of model group,Shuangdan Mingmu group and positive control group were lower than those of the normal group, and the average optical density were higher than those of the normal group. There was a significant difference between the model group and the normal group( P < 0. 01). The average gray values of VEGF-a,VEGF-b and VEGF-c expression in Shuangdan Mingmu group and positive control group were higher than those in the model group( P<0. 05) and the average optical density value were lower than those in the model group.( P<0. 01). CONCLUSION: Shuangdan Mingmu capsule could significantly reduce the expressions of VEGF-a,VEGF-b,VEGF-c in the retina and had a certain protective effect on the retina of rats in the diabetic retinopathy model.
AIM: To study the effects of Shuangdan Mingmu capsule on the expressions of vascular endothelial growth factor-a( VEGF-a),VEGF-b,VEGF-c in the retina of a diabetic rat model. METHODS: Forty male SD rats were divided into Group A( normal group),Group B( model group),Group C( Shuangdan Mingmu group) and Group D( positive control group) 10 rats( 20 eyes) in each group.A rat model of diabetic retinopathy was established by one-time tail vein injection with STZ( 50 mg/kg). After modeling for 1 wk, the rats were given medicine by gavage. After gavage for 4 wk rats were sacrificed,and the expressions of VEGF-a,VEGF-b,VEGF-c in the retina tissues were detected by immunohistochemical method. RESULTS: After gavage for 4 wk the average gray values of VEGF-a,VEGF-b and VEGF-c protein in the retina of model group,Shuangdan Mingmu group and positive control group were lower than those of the normal group, and the average optical density were higher than those of the normal group. There was a significant difference between the model group and the normal group( P < 0. 01). The average gray values of VEGF-a,VEGF-b and VEGF-c expression in Shuangdan Mingmu group and positive control group were higher than those in the model group( P<0. 05) and the average optical density value were lower than those in the model group.( P<0. 01). CONCLUSION: Shuangdan Mingmu capsule could significantly reduce the expressions of VEGF-a,VEGF-b,VEGF-c in the retina and had a certain protective effect on the retina of rats in the diabetic retinopathy model.
引文
1秦裕辉,李文娟,张熙,等.双丹明目胶囊对DR大鼠视网膜血管形态学及VEGF表达的影响.国际眼科杂志2015; 15(1):29-33
2 秦裕辉,李文娟,张熙,等.双丹明目胶囊对糖尿病视网膜病变大鼠血糖及视网膜功能的影响.国际眼科杂志2014; 14(11):1943-1945
3 秦裕辉,李文娟,张熙,等.双丹明目胶囊对糖尿病视网膜病变大鼠视网膜VEGF和VEGFR蛋白表达的影响.湖南中医药大学学报2015; 35(6):1-3
4 彭俊.双丹明目胶囊对糖尿病视网膜病变大鼠模型视网膜VEGFa、VEGF-b、VEGF-c及其受体Flk-1表达的影响.湖南中医药大学2017
5 彭清华.中医眼科学.北京:中国中医药出版社2012:187
6 秦裕辉,李芳,涂良钰,等.双丹明目胶囊治疗糖尿病视网膜病变的多中心临床研究.湖南中医药大学学报2010; 30(1):46-51
7 涂良钰,王文长,王育良,等.双丹明目胶囊治疗糖尿病视网膜病变的临床研究.中国新药杂志2009; 18(24):2331-2336
8 庞有慧.双丹明目胶囊治疗糖尿病视网膜病变的临床观察.糖尿病新世界2015; 3:39
9 Ferrara N,Henzel WJ. Pituitary follicular cells secrete a novel heparinbinding growth factor specific for vascular endothelial cells. Biochem Biophys Res Commun 1989; 161(2):851-858
10 Shams N, Ianchulev T. Role of vascular endothelial growth factor in ocular angiogenesis. Ophthalmol Clin North Am 2006; 19(3):335-344
11 路春,施彩虹. VEGF家族及其受体与糖尿病视网膜病变发病的关系.国际眼科杂志2007; 7(5):1400-1402
12 Nagy JA,Benjamin L,Zeng H, et al. Vascular permeability,vascular hyperpermeability and angiogenesis. Angiogenesis 2008; 11(2):109-119
13 张东梅,卿晨.血管内皮生长因子家族及其受体与肿瘤血管新生.医学综述2017; 23(3):417-420
2 秦裕辉,李文娟,张熙,等.双丹明目胶囊对糖尿病视网膜病变大鼠血糖及视网膜功能的影响.国际眼科杂志2014; 14(11):1943-1945
3 秦裕辉,李文娟,张熙,等.双丹明目胶囊对糖尿病视网膜病变大鼠视网膜VEGF和VEGFR蛋白表达的影响.湖南中医药大学学报2015; 35(6):1-3
4 彭俊.双丹明目胶囊对糖尿病视网膜病变大鼠模型视网膜VEGFa、VEGF-b、VEGF-c及其受体Flk-1表达的影响.湖南中医药大学2017
5 彭清华.中医眼科学.北京:中国中医药出版社2012:187
6 秦裕辉,李芳,涂良钰,等.双丹明目胶囊治疗糖尿病视网膜病变的多中心临床研究.湖南中医药大学学报2010; 30(1):46-51
7 涂良钰,王文长,王育良,等.双丹明目胶囊治疗糖尿病视网膜病变的临床研究.中国新药杂志2009; 18(24):2331-2336
8 庞有慧.双丹明目胶囊治疗糖尿病视网膜病变的临床观察.糖尿病新世界2015; 3:39
9 Ferrara N,Henzel WJ. Pituitary follicular cells secrete a novel heparinbinding growth factor specific for vascular endothelial cells. Biochem Biophys Res Commun 1989; 161(2):851-858
10 Shams N, Ianchulev T. Role of vascular endothelial growth factor in ocular angiogenesis. Ophthalmol Clin North Am 2006; 19(3):335-344
11 路春,施彩虹. VEGF家族及其受体与糖尿病视网膜病变发病的关系.国际眼科杂志2007; 7(5):1400-1402
12 Nagy JA,Benjamin L,Zeng H, et al. Vascular permeability,vascular hyperpermeability and angiogenesis. Angiogenesis 2008; 11(2):109-119
13 张东梅,卿晨.血管内皮生长因子家族及其受体与肿瘤血管新生.医学综述2017; 23(3):417-420