藏药十八味诃子利尿丸对糖尿病大鼠的保护作用
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摘要
目的探究十八味诃子利尿丸的降糖作用。方法两种不同时间间隔的多次给药方法建立四氧嘧啶糖尿病大鼠模型,血糖测定评估造模效果;成模后,观测十八味诃子利尿丸对大鼠摄食量、饮水量、尿量、体重、血糖、胰岛素水平、己糖激酶、琥珀酸脱氢酶、超氧化物歧化酶活力、丙二醛含量的影响,评估降糖效能。结果 24 h间隔给药法造模可使血糖显著升高(P<0. 05),且符合糖尿病标准;十八味诃子利尿丸可改善糖尿病大鼠"三多一少"症状(P<0. 05),降低血糖及丙二醛含量(P<0. 05),增加己糖激酶、琥珀酸脱氢酶及超氧化物歧化酶活力(P<0. 05),提高胰岛素水平(P<0. 05)。结论 24 h间隔给药法可建立四氧嘧啶糖尿病大鼠模型;十八味诃子利尿丸具有良好的降糖效果,其机制可能与促进机体对葡萄糖的摄取利用、提高抗氧化能力、减轻胰岛的损伤关系密切。
Objective To explore the hypoglycemic effect of eighteen flavor myrobalan diuretic pills on diabetic.Methods Adopting two kinds of methods to establish diabetic rat model,the result was evaluated by measuring the level of fasting blood glucose(FBG).Then the hypoglycemic effect of eighteen flavor myrobalan diuretic pills on diabetes was evaluated by testing food intake,water intake,urinary volume,weight,and the levels of FBG,fasting insulin(FINS),hexokinase(HK),succinate dehydrogenase(SDH),superoxide dismutase(SOD),malondialdehyde(MDA)and histopathology of islet in diabetic rats.Results The level of FBG was increased significantly(P<0.05)and in accordance with the diabetic standard by adopting the administering method of 24 h interval to establish diabetic rat model.Eighteen flavor myrobalan diuretic pills decreased food intake,water intake,urinary volume,the level of FBG,the content of MDA(P<0.05);and increased weight,the level of FINS,the activities of HK,SDH,SOD(P<0.05).It also reduced islet injury.Conclusions The administering method of 24 h interval could establish diabetic rat model successfully.And eighteen flavor myrobalan diuretic pills have good hypoglycemic effect,which relates with promoting the body to use the uptake of glucose and improving antioxidant capacity to reduce islet damage.
Objective To explore the hypoglycemic effect of eighteen flavor myrobalan diuretic pills on diabetic.Methods Adopting two kinds of methods to establish diabetic rat model,the result was evaluated by measuring the level of fasting blood glucose(FBG).Then the hypoglycemic effect of eighteen flavor myrobalan diuretic pills on diabetes was evaluated by testing food intake,water intake,urinary volume,weight,and the levels of FBG,fasting insulin(FINS),hexokinase(HK),succinate dehydrogenase(SDH),superoxide dismutase(SOD),malondialdehyde(MDA)and histopathology of islet in diabetic rats.Results The level of FBG was increased significantly(P<0.05)and in accordance with the diabetic standard by adopting the administering method of 24 h interval to establish diabetic rat model.Eighteen flavor myrobalan diuretic pills decreased food intake,water intake,urinary volume,the level of FBG,the content of MDA(P<0.05);and increased weight,the level of FINS,the activities of HK,SDH,SOD(P<0.05).It also reduced islet injury.Conclusions The administering method of 24 h interval could establish diabetic rat model successfully.And eighteen flavor myrobalan diuretic pills have good hypoglycemic effect,which relates with promoting the body to use the uptake of glucose and improving antioxidant capacity to reduce islet damage.
引文
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2 王会玲,李燕,胡伟锋,等.小檗碱影响AMPK/PGG-1信号途径改善糖尿病胰岛素抵抗和线粒体功能的研究[J].中华临床医师杂志,2014; 8(5):896-900.
3 梁沛余,尹磊晶,华欠桑多,等.多次给药法建立四氧嘧啶糖尿病大鼠模型的研究[J].青海医学院学报,2015; 36(4):276-9.
4 张丽娜,赵文红,谢岩黎,等.全麦粉对实验型糖尿病大鼠血糖控制效应的影响[J].食品科技,2015; 40(11):134-8.
5 Zou C,Qiu Q,Chen H,et al.Hepatoprotective effects of selenium during diabetes in rats[J].Human Exp Toxicol,2016; 35(2):114-23.
6 张奇峰.参芪降糖颗粒对糖尿病大鼠氧化应激反应的影响[J].中医药临床杂志,2016; 28(2):251-3.
7 Samarghandian S,Azimi-Nezhad M,Samini F,et al.Chrysin treatment improves diabetes and its complications in liver,brain,and pancreas in streptozotocin-induced diabetic rats[J]. Can J Physiol Pharmacol,2016; 94(4):388-93.
8 Hasnain SZ,Prins JB,McG uckin MA. Oxidative and endoplasmic reticulum stress inβ-cell dysfunction in diabetes[J].J Mol Endocrinol,2016; 56(2):33-54.
9 Zheng S,Zhao M,Wu Y,et al.Suppression of pancreatic beta cell apoptosis by Danzhi Jiangtang capsule contributes to the attenuation of type 1 diabetes in rats[J]. BMC Complement Altern Med,2016; 16(1):31.
10 任春久,张瑶,崔为正,等.氧化应激在2型糖尿病发病机制中的作用研究进展[J].生理学报,2013; 65(6):664-73.
11 段晋宁,肖旺,曾建红,等.莪术多糖对糖尿病大鼠血糖、抗脂质过氧化作用的影响与单糖组分分析[J].时珍国医国药,2016; 27(3):569-72.
12 Kim BH,Lee ES,Choi R,et al.Protective effect of curcumin on renal oxidative stress and lipid metabolism in a rat model of type 2 diabetic nephropathy[J].Yonsei Med J,2016; 57(3):664-73.
2 王会玲,李燕,胡伟锋,等.小檗碱影响AMPK/PGG-1信号途径改善糖尿病胰岛素抵抗和线粒体功能的研究[J].中华临床医师杂志,2014; 8(5):896-900.
3 梁沛余,尹磊晶,华欠桑多,等.多次给药法建立四氧嘧啶糖尿病大鼠模型的研究[J].青海医学院学报,2015; 36(4):276-9.
4 张丽娜,赵文红,谢岩黎,等.全麦粉对实验型糖尿病大鼠血糖控制效应的影响[J].食品科技,2015; 40(11):134-8.
5 Zou C,Qiu Q,Chen H,et al.Hepatoprotective effects of selenium during diabetes in rats[J].Human Exp Toxicol,2016; 35(2):114-23.
6 张奇峰.参芪降糖颗粒对糖尿病大鼠氧化应激反应的影响[J].中医药临床杂志,2016; 28(2):251-3.
7 Samarghandian S,Azimi-Nezhad M,Samini F,et al.Chrysin treatment improves diabetes and its complications in liver,brain,and pancreas in streptozotocin-induced diabetic rats[J]. Can J Physiol Pharmacol,2016; 94(4):388-93.
8 Hasnain SZ,Prins JB,McG uckin MA. Oxidative and endoplasmic reticulum stress inβ-cell dysfunction in diabetes[J].J Mol Endocrinol,2016; 56(2):33-54.
9 Zheng S,Zhao M,Wu Y,et al.Suppression of pancreatic beta cell apoptosis by Danzhi Jiangtang capsule contributes to the attenuation of type 1 diabetes in rats[J]. BMC Complement Altern Med,2016; 16(1):31.
10 任春久,张瑶,崔为正,等.氧化应激在2型糖尿病发病机制中的作用研究进展[J].生理学报,2013; 65(6):664-73.
11 段晋宁,肖旺,曾建红,等.莪术多糖对糖尿病大鼠血糖、抗脂质过氧化作用的影响与单糖组分分析[J].时珍国医国药,2016; 27(3):569-72.
12 Kim BH,Lee ES,Choi R,et al.Protective effect of curcumin on renal oxidative stress and lipid metabolism in a rat model of type 2 diabetic nephropathy[J].Yonsei Med J,2016; 57(3):664-73.