石菖蒲中生物碱成分的分离与鉴定
|
摘要
目的:分离并鉴定石菖蒲的生物碱成分,为进一步研究其药理活性奠定基础。方法:利用阳离子交换树脂分离石菖蒲的生物碱成分;采用超高效液相色谱串联质谱(UPLC-MS)法进行鉴定,色谱柱为Agilent Zorbax Eclipse Plus-C18,流动相为0.1%甲酸水溶液-乙腈(梯度洗脱),流速为0.3 mL/min,柱温为30℃,进样量为1μL;离子源为电喷雾离子源,采用正离子模式检测,并结合Peakview 1.2等软件对生物碱成分进行分析。结果:石菖蒲中共检测到86个成分,并鉴定出5个生物碱成分,分别为菖蒲碱甲、N-反式阿魏酰酪胺、N-月桂基二乙醇胺、4-羟双氢鞘氨醇和5-丁基尿苷。结论:该试验结果可为石菖蒲生物碱成分的药理活性研究奠定基础。
OBJECTIVE:To isolate and identify the compositions of alkaloids in Acorus tatarinowii,and to lay the foundation for further study of its pharmacological activity. METHODS:The compositions of alkaloids from A. tatarinowii was separated by cation exchange resin. UPLC-MS method was used to identify the alkaloids. The separation was performed on a Agilent Zorbax Eclipse Plus-C18 column with mobile phase consisted of 0.1% formic acid-acetonitrile(gradient elution) at a flow rate of 0.3 mL/min. The column temperature was 30 ℃,and the injection volume was 1 μL. ESI ion source was used to detect the fragments,the ion mode scan was positive. Peakview 1.2 and other softwares were used to analyze the compositions of alkaloids. RESULTS:86 compositions were separated and 5 alkaloids were identified,such as tatarine A,N-trans-feruloyltyramine,N-lauryldiethanolamine,phytosphingosine and 5-butyluridine. CONCLUSIONS:The results of the study lay a foundation for the study of pharmacological activity of alkaloids from A. tatarinowii.
OBJECTIVE:To isolate and identify the compositions of alkaloids in Acorus tatarinowii,and to lay the foundation for further study of its pharmacological activity. METHODS:The compositions of alkaloids from A. tatarinowii was separated by cation exchange resin. UPLC-MS method was used to identify the alkaloids. The separation was performed on a Agilent Zorbax Eclipse Plus-C18 column with mobile phase consisted of 0.1% formic acid-acetonitrile(gradient elution) at a flow rate of 0.3 mL/min. The column temperature was 30 ℃,and the injection volume was 1 μL. ESI ion source was used to detect the fragments,the ion mode scan was positive. Peakview 1.2 and other softwares were used to analyze the compositions of alkaloids. RESULTS:86 compositions were separated and 5 alkaloids were identified,such as tatarine A,N-trans-feruloyltyramine,N-lauryldiethanolamine,phytosphingosine and 5-butyluridine. CONCLUSIONS:The results of the study lay a foundation for the study of pharmacological activity of alkaloids from A. tatarinowii.
引文
[1]黄月纯,魏刚.石菖蒲挥发油的气相色谱指纹图谱研究[J].中国药房,2005,16(21):1676-1677.
[2]吴启端,吴清和.石菖蒲的药理研究进展[J].中药新药与临床药理,2006,17(6):477-480.
[3]胡增峣,黄晏,刘港,等.中药复方治疗老年痴呆的用药规律分析[J].中药药理与临床,2012,28(5):252-256.
[4]杨洁,陶汝俊,王垠芸,等.石菖蒲化学成分及其对中枢神经系统作用研究进展[J].药物资讯,2017,6(5):133-141.
[5]卢成淑,冯宁,南国,等.石菖蒲及其活性成分防治阿尔茨海默病的研究进展[J].中草药,2016,47(7):1236-1242.
[6]倪刚.石菖蒲、华桑化学成分及生物活性研究[D].北京:北京协和医学院,2010.
[7]陈锋.菖蒲属植物的化学成分及药理作用[J].世界科学技术(中医药现代化),2011,13(6):1013-1017.
[8]劳安娜,唐希灿,王洪诚,等.石菖蒲中菖蒲碱及它们的用途:中国,ZL97106786.4[P].2013-02-19.
[9]YUAN J,CHEN Y,LIANG J,et al.Component analysis and target cell-based neuroactivity screening of panax ginseng by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry[J].JChromatogr B Analyt Technol Biomed Life Sci,2016.DOI:10.1016/j.jchromb.2016.10.014
[10]吴春华,陈玥,李晓霞,等.石菖蒲化学成分的分离与结构鉴定[J].中国药物化学杂志,2014,24(3):209-213.
[11]KUMAR V,KUMAR S,SINGH B,et al.Quantitative and structural analysis of amides and lignans in zonthoxylum armatum by UPLC-DAD-ESI-QTOF-MS/MS[J].JPharmaceut Biomed Anal,2014,94(3):23-29.
[12]杨颖达.3种植物的化学成分和生物活性研究[D].武汉:华中科技大学,2014.
[13]赖显英,梁红,赵玉英.石菖蒲化学成分和药理作用研究[J].中国中药杂志,2002,27(3):161-166.
[14]唐怡,任刚,黄群,等.石菖蒲挥发油化学成分的GC-MS[J].江西中医药,2014,45(12):60-62.
[15]唐怡,李健康,刘校妃,等.石菖蒲鲜、干药材及其不同部位中挥发油、α-细辛醚和β-细辛醚的含量比较[J].中国实验方剂学杂志,2016,22(5):32-35.
[16]吴启端,方永奇,李翎,等.不同剂量石菖蒲对中枢神经系统影响的实验研究[J].中国药房,2005,16(9):656-658.
[2]吴启端,吴清和.石菖蒲的药理研究进展[J].中药新药与临床药理,2006,17(6):477-480.
[3]胡增峣,黄晏,刘港,等.中药复方治疗老年痴呆的用药规律分析[J].中药药理与临床,2012,28(5):252-256.
[4]杨洁,陶汝俊,王垠芸,等.石菖蒲化学成分及其对中枢神经系统作用研究进展[J].药物资讯,2017,6(5):133-141.
[5]卢成淑,冯宁,南国,等.石菖蒲及其活性成分防治阿尔茨海默病的研究进展[J].中草药,2016,47(7):1236-1242.
[6]倪刚.石菖蒲、华桑化学成分及生物活性研究[D].北京:北京协和医学院,2010.
[7]陈锋.菖蒲属植物的化学成分及药理作用[J].世界科学技术(中医药现代化),2011,13(6):1013-1017.
[8]劳安娜,唐希灿,王洪诚,等.石菖蒲中菖蒲碱及它们的用途:中国,ZL97106786.4[P].2013-02-19.
[9]YUAN J,CHEN Y,LIANG J,et al.Component analysis and target cell-based neuroactivity screening of panax ginseng by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry[J].JChromatogr B Analyt Technol Biomed Life Sci,2016.DOI:10.1016/j.jchromb.2016.10.014
[10]吴春华,陈玥,李晓霞,等.石菖蒲化学成分的分离与结构鉴定[J].中国药物化学杂志,2014,24(3):209-213.
[11]KUMAR V,KUMAR S,SINGH B,et al.Quantitative and structural analysis of amides and lignans in zonthoxylum armatum by UPLC-DAD-ESI-QTOF-MS/MS[J].JPharmaceut Biomed Anal,2014,94(3):23-29.
[12]杨颖达.3种植物的化学成分和生物活性研究[D].武汉:华中科技大学,2014.
[13]赖显英,梁红,赵玉英.石菖蒲化学成分和药理作用研究[J].中国中药杂志,2002,27(3):161-166.
[14]唐怡,任刚,黄群,等.石菖蒲挥发油化学成分的GC-MS[J].江西中医药,2014,45(12):60-62.
[15]唐怡,李健康,刘校妃,等.石菖蒲鲜、干药材及其不同部位中挥发油、α-细辛醚和β-细辛醚的含量比较[J].中国实验方剂学杂志,2016,22(5):32-35.
[16]吴启端,方永奇,李翎,等.不同剂量石菖蒲对中枢神经系统影响的实验研究[J].中国药房,2005,16(9):656-658.