水分胁迫对‘翠鸟’玉簪叶片表皮蜡质及生理特性的影响
|
摘要
为探究土壤水分对阴生植物玉簪叶片表皮蜡质及生长的影响,以蓝叶玉簪‘翠鸟’(Hosta ‘Halcyon’)为试材,采用盆栽方法,设置正常给水、轻度、中度和重度胁迫4种土壤水分处理,研究其叶片表皮蜡质和生理特性的变化,并对它们之间的关系进行了分析。结果表明:在一定范围内,随着干旱程度的增加,表皮蜡质的量随之增大;本研究中,中度胁迫的蜡质最多,其光合速率、蒸腾速率、气孔导度等处于较低水平,表明‘翠鸟’玉簪在干旱胁迫下通过减小气孔开度、减少蒸腾速率及叶片水分散失,以提高水分利用效率,从而增强自身的抗旱性;随着胁迫加剧,光合速率、水分利用效率及叶绿素含量明显下降,说明叶绿素合成受到抑制。叶片表皮蜡质的增加,减少了叶片表面通透性,从而降低水分散失,以减少干旱对植株生长的不利影响。此外还发现,蜡质的多少直接影响植物叶片的颜色。中度水分胁迫时,其蜡质最多,同时叶绿素含量较高,所以叶片蓝绿色显著深于重度胁迫处理,观赏价值较高。
In order to study the effects of soil water on the epicuticular wax and the growth of shade plant leaves,the blue leaf variety of Hosta‘Halcyon'was grown in pots with 4 kinds of soil water treatments,which were normal water supply,light water stress treatment,medium water stress treatment and severe water stress treatment. The changes of epicuticular wax and physiological characteristics of Hosta‘Halcyon'leaves were studied under different water stress,and the relationships between them were analyzed. The results showed that the epicuticular wax increased with the increase of drought,and the photosynthetic rate,transpiration rate and stomatal conductance of the medium stress treatment were at low level within a certain drought range,which indicated that the Hosta‘Halcyon'improved water use efficiency and enhanced drought resistance by reducing stomatal opening,transpiration rate and leaf water loss under water stress. With the further intensity of water stress,photosynthesis rate,water use efficiency and leaf chlorophyll content decreased significantly,indicating that chlorophyll synthesis was inhibited,and the increase of leaf epicuticular wax decreased leaf surface permeability,thus reducing water dispersion loss in order to reduce the adverse effects of drought on plant growth. In addition,the study showed that the wax directly affected the color of the leaves. The medium stress group had the highest wax content and higher chlorophyll content,so the blue-green leaf was significantly deeper than the severe stress group,and the ornamental value was higher.
In order to study the effects of soil water on the epicuticular wax and the growth of shade plant leaves,the blue leaf variety of Hosta‘Halcyon'was grown in pots with 4 kinds of soil water treatments,which were normal water supply,light water stress treatment,medium water stress treatment and severe water stress treatment. The changes of epicuticular wax and physiological characteristics of Hosta‘Halcyon'leaves were studied under different water stress,and the relationships between them were analyzed. The results showed that the epicuticular wax increased with the increase of drought,and the photosynthetic rate,transpiration rate and stomatal conductance of the medium stress treatment were at low level within a certain drought range,which indicated that the Hosta‘Halcyon'improved water use efficiency and enhanced drought resistance by reducing stomatal opening,transpiration rate and leaf water loss under water stress. With the further intensity of water stress,photosynthesis rate,water use efficiency and leaf chlorophyll content decreased significantly,indicating that chlorophyll synthesis was inhibited,and the increase of leaf epicuticular wax decreased leaf surface permeability,thus reducing water dispersion loss in order to reduce the adverse effects of drought on plant growth. In addition,the study showed that the wax directly affected the color of the leaves. The medium stress group had the highest wax content and higher chlorophyll content,so the blue-green leaf was significantly deeper than the severe stress group,and the ornamental value was higher.
引文
Bi H,Kovalchuk N,Langridge P,Tricker P J,Lopato S,Borisjuk N.2017.The impact of drought on wheat leaf cuticle properties.BMC Plant Biology,17(1):85.
Dai Hui,Fang Yan-ming,Li Bin,Zhang Min.2014.Research review and prospect of Hosta species.Journal of Jiangsu Forestry Science&Technology,41(3):37-41.(in Chinese)代慧，方炎明，利斌，张敏.2014.玉簪属植物研究概况与展望.江苏林业科技，41(3):37-41.
Grenfell D.1996.The gardener’s guide to growing Hosta.Portland,Oregon:Timeber Press.
Guan Meng-qian,Dong Ran.2013.Research progress and garden application of Hosta species.Northern Horticulture,(19):182-185.(in Chinese)关梦茜，董然.2013.玉簪属植物研究进展及园林应用.北方园艺，(19):182-185.
Guo Yan-jun,Guo Yun-jiang,Tang Hua,Li Zhi-yan,Han Long.2011.Ultraviolet radiation and soil drought stress on alfalfa effects of leaf crystal structure and content on leaf epidermis.Acta Prataculturae Sinica,20(6):77-84.(in Chinese)郭彦军，郭芸江，唐华，李智燕，韩龙.2011.紫外线辐射与土壤干旱胁迫对紫花苜蓿叶表皮蜡质晶体结构及含量的影响.草业学报，20(6):77-84.
Huang Ling,Zhang Zheng-bin,Cui Yu-ting,Liu Meng-yu,Chai Shou-xi,Chen Zhao-bo.2003.Relationship between wax content and water use efficiency of leaf and yield in wheat.Journal of Triticeae Crops,23(3):41-44.(in Chinese)黄玲，张正斌，崔玉亭，刘孟雨，柴守玺，陈兆波.2003.小麦叶片蜡质含量与水分利用效率和产量的关系.麦类作物学报，23(3):41-44.
Islam M A,Du H,Ning J,Ye H Y,Xiong L Z.2009.Characterization of Glossy1-homologous genes in rice involved in leaf wax accumulation and drought resistance.Plant Mol Biol,70(4):443-456.
Jenks M A,Gaston C H,Goodwin M S,Keith J A,Teusink R S.2002.Seasonal variation in culticular waxes on Hosta genotypes differing in leaf surface glaucousness.HortScience,37(4):673-677.
Kosma D K,Bourden X B,Bernard A L,Parsons E P,LüS,Joubès J,Jenks M A.2009.The impact of water deficiency on leaf cuticle lipids of Arabidopsis.Plant Physiology,151(4):1918-1929.
Li De-lu,Liu Shi-zheng,Kang Cai-zhou,Yan Zi-zhu,Man Duo-qing,Liu You-jun,Li Yin-ke.2015.Characteristics of fluorescence parameters of two species of picea in water stress.Journal of Arid Land Resources and Environment,29(6):117-121.(in Chinese)李得禄，刘世增，康才周，严子柱，满多清，刘有军，李银科.2015.水分胁迫下云杉属两种植物荧光参数特征研究.干旱区资源与环境，29(6):117-121.
Macherius A,Kuschk P,Haertig C,Moeder M,Perez P,Shtemenko N I,Bayona A H,Guerrero H,Gey M.2001.Composition changes in the cuticular surface lipids of the helophytes Phragmites australis and Juncus effuses as result of pollutant exposure.Environmental Science and Pollution Research,18(5):727-733.
Mo Wei-ping,Yan Ting-ting,Cui Chun-mei,Wang Bo-qian,Xing Si-nian,Jia Wen-suo,Liu Guo-jie.2015.Effects of water stress on photochemical efficiency of PSⅡand light energy allocation in Vitis vinifera Kyoho grape.Journal of China Agricultural University,20(3):59-64.(in Chinese)莫伟平，严婷婷，崔春梅，王博茜，邢思年，贾文锁，刘国杰.2015.水分胁迫对巨峰葡萄叶片PSⅡ光化学效率及光能分配的影响.中国农业大学学报，20(3):59-64.
Qi C H,Jiang H,Zhao X Y,Mao K,Liu H T,Li Y Y,Hao Y J.2019.The characterization,authentication,and gene expression pattern of the MdCER family in Malus domestica.Horticultural Plant Journal,5(1):1-9.
Riederer M,Schneider G.1990.The effect of the environment on the permeability and composition of citrus leaf cuticles.Planta,180(2):154-165.
Samuels L,Kunst L,Jetter R.2008.Sealing plant surfaces:cuticular wax formation by epidermal cells.Annual Review of Plant Biology,59:683-707.
Shi Ai-ping,Zhang Jin-zheng,Zhang Qi-xiang,Shi Lei.2004.Growth characteristic analyse of shading levels on four cultivars.Bulletin of Botanical Research,24(4):486-490.(in Chinese)施爱萍，张金政，张启翔，石雷.2004.不同遮荫水平4个玉簪品种的生长性状分析.植物研究，24(4):486-490.
Wang Qiu-ping,Gong Jia-shun,Zou Sha-sha.2010.Relationship between color change and quality of Pu-erh tea during fermentation.Transactions of the Chinese Society of Agricultural Engineering,26(Supp 1):394-399.(in Chinese)王秋萍，龚加顺，邹莎莎.2010.普洱茶发酵阶段色泽的变化及其与品质的关系.农业工程学报，26(增刊1):394-399.
Wei Bin,Chen Qi-xin.1996.The method of using colorimetric theory to improve the visual perception of map color.Journal of PLA Institute of Surveying and Mapping,13(1):50-54.(in Chinese)魏斌，陈启鑫.1996.运用色度学理论提高地图颜色视觉感受的方法.解放军测绘学院学报，13(1):50-54.
Wu Hong-qi,Luo Wen-Qiao,Zhao Shuai,Wang Cong,Ni Zhen,Zhu Hao,Niu Yan-lu,Wang Yong,Wang Zhong-hua,Quan Li.2017.Effect of drought stress on wax accumulation in leaves of tomato(Lycopersicon esculentum).Journal of Northwest A&F University(Nat Sci Ed),45(7):73-80.(in Chinese)吴洪启，罗文巧，赵帅，王聪，倪震，朱浩，牛艳璐，汪勇，王中华，权力.2017.干旱胁迫对番茄叶片蜡质积累的影响.西北农林科技大学学报（自然科学版），45(7):73-80.
Ye Xiao-li,Li Xue-gang,Chen Shi-hong.2000.Effect of surfactants on stomatal and waxy layer of soybean leaves.Soybean Science,19(1):49-56.(in Chinese)叶小利，李学刚，陈时洪.2000.表面活性剂对大豆叶面气孔和蜡质层影响研究．大豆科学，19(1):49-56．
Zilis M R.2000.The Hosta handbook.Rochelle IL:Q&Z Nusery:18-19.
Zhu Shuang-yan,Qi Jun-cang,Liao Le,Lin Li-hao,Hui Hong-shan,Hua Miao-yuan.2015.PEG-6000 simulated barley seedling leaves under water stress study on the relationship between epidermal wax deposition and membrane lipid peroxidation.Jiangsu Agricultural Science,43(5):89-92.(in Chinese)朱双艳，齐军仓，廖乐，林立昊，惠宏杉，华淼源.2015.PEG-600模拟水分胁迫下大麦幼苗叶片表皮蜡质沉积与膜脂过氧化的关系研究.江苏农业科学，43(5):89-92.
Dai Hui,Fang Yan-ming,Li Bin,Zhang Min.2014.Research review and prospect of Hosta species.Journal of Jiangsu Forestry Science&Technology,41(3):37-41.(in Chinese)代慧，方炎明，利斌，张敏.2014.玉簪属植物研究概况与展望.江苏林业科技，41(3):37-41.
Grenfell D.1996.The gardener’s guide to growing Hosta.Portland,Oregon:Timeber Press.
Guan Meng-qian,Dong Ran.2013.Research progress and garden application of Hosta species.Northern Horticulture,(19):182-185.(in Chinese)关梦茜，董然.2013.玉簪属植物研究进展及园林应用.北方园艺，(19):182-185.
Guo Yan-jun,Guo Yun-jiang,Tang Hua,Li Zhi-yan,Han Long.2011.Ultraviolet radiation and soil drought stress on alfalfa effects of leaf crystal structure and content on leaf epidermis.Acta Prataculturae Sinica,20(6):77-84.(in Chinese)郭彦军，郭芸江，唐华，李智燕，韩龙.2011.紫外线辐射与土壤干旱胁迫对紫花苜蓿叶表皮蜡质晶体结构及含量的影响.草业学报，20(6):77-84.
Huang Ling,Zhang Zheng-bin,Cui Yu-ting,Liu Meng-yu,Chai Shou-xi,Chen Zhao-bo.2003.Relationship between wax content and water use efficiency of leaf and yield in wheat.Journal of Triticeae Crops,23(3):41-44.(in Chinese)黄玲，张正斌，崔玉亭，刘孟雨，柴守玺，陈兆波.2003.小麦叶片蜡质含量与水分利用效率和产量的关系.麦类作物学报，23(3):41-44.
Islam M A,Du H,Ning J,Ye H Y,Xiong L Z.2009.Characterization of Glossy1-homologous genes in rice involved in leaf wax accumulation and drought resistance.Plant Mol Biol,70(4):443-456.
Jenks M A,Gaston C H,Goodwin M S,Keith J A,Teusink R S.2002.Seasonal variation in culticular waxes on Hosta genotypes differing in leaf surface glaucousness.HortScience,37(4):673-677.
Kosma D K,Bourden X B,Bernard A L,Parsons E P,LüS,Joubès J,Jenks M A.2009.The impact of water deficiency on leaf cuticle lipids of Arabidopsis.Plant Physiology,151(4):1918-1929.
Li De-lu,Liu Shi-zheng,Kang Cai-zhou,Yan Zi-zhu,Man Duo-qing,Liu You-jun,Li Yin-ke.2015.Characteristics of fluorescence parameters of two species of picea in water stress.Journal of Arid Land Resources and Environment,29(6):117-121.(in Chinese)李得禄，刘世增，康才周，严子柱，满多清，刘有军，李银科.2015.水分胁迫下云杉属两种植物荧光参数特征研究.干旱区资源与环境，29(6):117-121.
Macherius A,Kuschk P,Haertig C,Moeder M,Perez P,Shtemenko N I,Bayona A H,Guerrero H,Gey M.2001.Composition changes in the cuticular surface lipids of the helophytes Phragmites australis and Juncus effuses as result of pollutant exposure.Environmental Science and Pollution Research,18(5):727-733.
Mo Wei-ping,Yan Ting-ting,Cui Chun-mei,Wang Bo-qian,Xing Si-nian,Jia Wen-suo,Liu Guo-jie.2015.Effects of water stress on photochemical efficiency of PSⅡand light energy allocation in Vitis vinifera Kyoho grape.Journal of China Agricultural University,20(3):59-64.(in Chinese)莫伟平，严婷婷，崔春梅，王博茜，邢思年，贾文锁，刘国杰.2015.水分胁迫对巨峰葡萄叶片PSⅡ光化学效率及光能分配的影响.中国农业大学学报，20(3):59-64.
Qi C H,Jiang H,Zhao X Y,Mao K,Liu H T,Li Y Y,Hao Y J.2019.The characterization,authentication,and gene expression pattern of the MdCER family in Malus domestica.Horticultural Plant Journal,5(1):1-9.
Riederer M,Schneider G.1990.The effect of the environment on the permeability and composition of citrus leaf cuticles.Planta,180(2):154-165.
Samuels L,Kunst L,Jetter R.2008.Sealing plant surfaces:cuticular wax formation by epidermal cells.Annual Review of Plant Biology,59:683-707.
Shi Ai-ping,Zhang Jin-zheng,Zhang Qi-xiang,Shi Lei.2004.Growth characteristic analyse of shading levels on four cultivars.Bulletin of Botanical Research,24(4):486-490.(in Chinese)施爱萍，张金政，张启翔，石雷.2004.不同遮荫水平4个玉簪品种的生长性状分析.植物研究，24(4):486-490.
Wang Qiu-ping,Gong Jia-shun,Zou Sha-sha.2010.Relationship between color change and quality of Pu-erh tea during fermentation.Transactions of the Chinese Society of Agricultural Engineering,26(Supp 1):394-399.(in Chinese)王秋萍，龚加顺，邹莎莎.2010.普洱茶发酵阶段色泽的变化及其与品质的关系.农业工程学报，26(增刊1):394-399.
Wei Bin,Chen Qi-xin.1996.The method of using colorimetric theory to improve the visual perception of map color.Journal of PLA Institute of Surveying and Mapping,13(1):50-54.(in Chinese)魏斌，陈启鑫.1996.运用色度学理论提高地图颜色视觉感受的方法.解放军测绘学院学报，13(1):50-54.
Wu Hong-qi,Luo Wen-Qiao,Zhao Shuai,Wang Cong,Ni Zhen,Zhu Hao,Niu Yan-lu,Wang Yong,Wang Zhong-hua,Quan Li.2017.Effect of drought stress on wax accumulation in leaves of tomato(Lycopersicon esculentum).Journal of Northwest A&F University(Nat Sci Ed),45(7):73-80.(in Chinese)吴洪启，罗文巧，赵帅，王聪，倪震，朱浩，牛艳璐，汪勇，王中华，权力.2017.干旱胁迫对番茄叶片蜡质积累的影响.西北农林科技大学学报（自然科学版），45(7):73-80.
Ye Xiao-li,Li Xue-gang,Chen Shi-hong.2000.Effect of surfactants on stomatal and waxy layer of soybean leaves.Soybean Science,19(1):49-56.(in Chinese)叶小利，李学刚，陈时洪.2000.表面活性剂对大豆叶面气孔和蜡质层影响研究．大豆科学，19(1):49-56．
Zilis M R.2000.The Hosta handbook.Rochelle IL:Q&Z Nusery:18-19.
Zhu Shuang-yan,Qi Jun-cang,Liao Le,Lin Li-hao,Hui Hong-shan,Hua Miao-yuan.2015.PEG-6000 simulated barley seedling leaves under water stress study on the relationship between epidermal wax deposition and membrane lipid peroxidation.Jiangsu Agricultural Science,43(5):89-92.(in Chinese)朱双艳，齐军仓，廖乐，林立昊，惠宏杉，华淼源.2015.PEG-600模拟水分胁迫下大麦幼苗叶片表皮蜡质沉积与膜脂过氧化的关系研究.江苏农业科学，43(5):89-92.