银杏叶提取物EGB761对AS家兔PON-1和ox-LDL的影响
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摘要
[目的]观察银杏叶提取物EGB761对动脉粥样硬化家兔对氧磷酶-1(PON-1)和氧化低密度脂蛋白(oxLDL)的影响。[方法]将24只日本大耳白兔随机分为正常组、模型组、EGB761组(n=8)。正常组给予普通饮食,模型组和EGB761组高脂饮食10周。EGB761组从第7周起给予EGB761 50 mg/(kg·d)灌胃4周,正常组和模型组给予等量生理盐水。采用酶免疫吸附法(ELISA法)和生化法检测PON-1的活性和含量,ELISA法检测ox-LDL的含量。[结果]模型组PON-1活性和含量明显降低(P<0.05),ox-LDL的含量明显升高,银杏叶提取物组PON-1活性和含量明显升高,ox-LDL明显降低(P<0.05)。[结论]银杏叶提取物EGB761降低ox-LDL、抗动脉粥样梗化(AS)的机制可能与其上调PON-1活性和含量有关。
[Objective] To observe effect of EGB761 on PON-1 and ox-LDL in rabbits with atherosclerosis.[Methods] Twenty-four Japanese white rabbits were randomly divided into normal group, model group and EGB761 group. Rabbits in normal group were fed with ordinary diet; model and EGB761 group were given high-fat diet for10 weeks. Meanwhile, from the seventh week, rabbits in EGB761 group were given 50 mg/(kg·d) of EGB761, control group and model group were given normal saline for 4 weeks. Ten weeks later, the vitality and content of PON-1 were detected by Elisa and biochemical methods, the ox-LDL were detected by Elisa method. [Results] Compared with the model group, the vitality and content of PON1 in model group were higher( P<0.05) and the ox-LDL levels were lower, while in EGB761 group the vitality and content of PON-1 increased significantly and ox-LDL decreased obviously(P<0.05). [Conclusion] The effect of EGB761 on reducing the level of ox-LDL in AS rabbits may response to raise the vitality and content of PON-1.
[Objective] To observe effect of EGB761 on PON-1 and ox-LDL in rabbits with atherosclerosis.[Methods] Twenty-four Japanese white rabbits were randomly divided into normal group, model group and EGB761 group. Rabbits in normal group were fed with ordinary diet; model and EGB761 group were given high-fat diet for10 weeks. Meanwhile, from the seventh week, rabbits in EGB761 group were given 50 mg/(kg·d) of EGB761, control group and model group were given normal saline for 4 weeks. Ten weeks later, the vitality and content of PON-1 were detected by Elisa and biochemical methods, the ox-LDL were detected by Elisa method. [Results] Compared with the model group, the vitality and content of PON1 in model group were higher( P<0.05) and the ox-LDL levels were lower, while in EGB761 group the vitality and content of PON-1 increased significantly and ox-LDL decreased obviously(P<0.05). [Conclusion] The effect of EGB761 on reducing the level of ox-LDL in AS rabbits may response to raise the vitality and content of PON-1.
引文
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[7]万冬宇,黄维义.银杏叶提取物抗动脉粥样硬化作用机制的研究进展[J].心血管病学进展,2010,31(1):131-133.
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[10]王强,马东明,张瑞峰.银杏叶提取物EGB761对高胆固醇血症家兔动脉粥样硬化的防治作用[J].江西中医学院学报,2009,21(6):45-47.
[11]Gaidukov L,Tawfik DS.High affinity,stability and lactonase activity of serum paraoxonase PON1 anchored on HDL with Apo AⅠ[J].Biochemistry,2005,44(35):11843-11854.
[12]Durrington PN,Mackness B,Mackness MI.Paraoxonase and Atherosclerosis[J].Arterioscler Thromb Vasc Biol,2001,214:473-480.
[13]Fuhrman B,Volkova N,Aviram M.Oxidative stress increases the expression of the CD36 scavenger receptor and the cellular uptake of oxidized low-density lipoprotein in macrophages from atherosclerotic mice:protective role of antioxidants and of paraoxonase[J].Atherosclerosis,2002,161(2):307-316.
[14]蒋兴亮,周京国,唐中,等.冠心病患者对氧磷脂酶1活性与氧化型低密度脂蛋白的关系[J].中华老年心脑血管病杂志,2004,6(5):305-307.
[15]伍海涛.EGb对高脂饮食所致大鼠血管功能损伤的保护作用与PON1关系的研究[D].长沙:中南大学,2003.
[16]Tian X,Zhang L,Wang J,et al.The protective effect of hyperbaric oxygen and Ginkgo biloba extract on Aβ25-35-induced oxidative stress and neuronal apoptosis in rat-s[J].Behav Brain Res,2013,242:1-8.
[17]Zhao Z,Liu N,Huang J,et al.Inhibition of c PLA2 activation by Ginkgo biloba extract protects spinal cord neurons from glutamate excitotoxicity and oxidative stress-induced cell death[J].Neurochem,2011,116(6):1057-1065.
[18]Kaur S,Chhabra R,Nehru B.Ginkgo biloba extract attenuates hippocampal neuronal loss and cognitive dysfunction resulting from trimethyltin in mice[J].Phytomedicine,2013,20(2):178-186.
[19]Jiang X,Nie B,Fu S.EGb761 protects hydrogen peroxideinduced death of spinal cord neurons through inhibition of intracellular ROS production and modulation of apoptotic regulating genes[J].Mol Neurosci,2009,38(2):103-113.
[20]邹健,蒋晓燕,徐晓明.银杏叶提取物EGb761神经保护作用研究进展[J].中草药,2005,36(11):1734-1736.
[21]Pierre SV,Lesnik P,Moreau M,et al.The standardized Ginkgo biloba extract Egb-761 protects vascular endothelium exposed to oxidized low density lipoproteins[J].Cell Mol Biol(Noisy-le-grand),2008,26(54 Suppl):OL1032-1042.
[22]孟庆楠,张皓楠,王益民.MTT法检测银杏叶注射液对人脑微血管内皮细胞增殖的影响[J].天津中医药大学学报,2011,30(1):36-40.
[23]徐倩,张艳军,刘洋,等.银杏内酯B作用于星形胶质细胞对神经干细胞趋化影响的体外研究[J].天津中医药,2010,27(5):421-424.
[2]Hegele RA.Paraoxonase genes and disease[J].Ann Med.1999,31(3):217-224.
[3]Gugliucci A,Menini T.Paraoxonase 1 and HDL maturation[J].Clin Chim Acta,2015,439:5-13.
[4]Hine D,Mackness B,Mackness M.Coincubation of PON1,APO A1,and LCAT increases the time HDL is able to prevent LDL oxidation[J].Iubmb Life,2012,64(2):157-161.
[5]彭薇,张弛,姜晓玲,等.对氧磷酶基因家族[J].医学分子生物学杂志,2007,4(1):63-66.
[6]Wei JM,Wang X,Gong H,et al.Ginkgo suppresses atherosclerosis through downregulating the expression of connexin 43 in rabbits[J].Arch Med Sci,2013,9(2):340-346.
[7]万冬宇,黄维义.银杏叶提取物抗动脉粥样硬化作用机制的研究进展[J].心血管病学进展,2010,31(1):131-133.
[8]Peluso I,Morabito G,Urban L,et al.Oxidative stress in atherosclerosis development:the central role of LDL and oxidative burst[J].Endocr Metab Immune Disord Drug Targets,2012,12(4):351-360.
[9]Bickering S,Quentin J,Jostens LA,et al.Oxidized lowdensity lipoprotein induces long-term proinflammatory cytokine production and foam cell formation via epigenetic reprogramming of monocots[J].Arterioscleroses Thrombi Vass Boil,2014,34(8):1731-1738.
[10]王强,马东明,张瑞峰.银杏叶提取物EGB761对高胆固醇血症家兔动脉粥样硬化的防治作用[J].江西中医学院学报,2009,21(6):45-47.
[11]Gaidukov L,Tawfik DS.High affinity,stability and lactonase activity of serum paraoxonase PON1 anchored on HDL with Apo AⅠ[J].Biochemistry,2005,44(35):11843-11854.
[12]Durrington PN,Mackness B,Mackness MI.Paraoxonase and Atherosclerosis[J].Arterioscler Thromb Vasc Biol,2001,214:473-480.
[13]Fuhrman B,Volkova N,Aviram M.Oxidative stress increases the expression of the CD36 scavenger receptor and the cellular uptake of oxidized low-density lipoprotein in macrophages from atherosclerotic mice:protective role of antioxidants and of paraoxonase[J].Atherosclerosis,2002,161(2):307-316.
[14]蒋兴亮,周京国,唐中,等.冠心病患者对氧磷脂酶1活性与氧化型低密度脂蛋白的关系[J].中华老年心脑血管病杂志,2004,6(5):305-307.
[15]伍海涛.EGb对高脂饮食所致大鼠血管功能损伤的保护作用与PON1关系的研究[D].长沙:中南大学,2003.
[16]Tian X,Zhang L,Wang J,et al.The protective effect of hyperbaric oxygen and Ginkgo biloba extract on Aβ25-35-induced oxidative stress and neuronal apoptosis in rat-s[J].Behav Brain Res,2013,242:1-8.
[17]Zhao Z,Liu N,Huang J,et al.Inhibition of c PLA2 activation by Ginkgo biloba extract protects spinal cord neurons from glutamate excitotoxicity and oxidative stress-induced cell death[J].Neurochem,2011,116(6):1057-1065.
[18]Kaur S,Chhabra R,Nehru B.Ginkgo biloba extract attenuates hippocampal neuronal loss and cognitive dysfunction resulting from trimethyltin in mice[J].Phytomedicine,2013,20(2):178-186.
[19]Jiang X,Nie B,Fu S.EGb761 protects hydrogen peroxideinduced death of spinal cord neurons through inhibition of intracellular ROS production and modulation of apoptotic regulating genes[J].Mol Neurosci,2009,38(2):103-113.
[20]邹健,蒋晓燕,徐晓明.银杏叶提取物EGb761神经保护作用研究进展[J].中草药,2005,36(11):1734-1736.
[21]Pierre SV,Lesnik P,Moreau M,et al.The standardized Ginkgo biloba extract Egb-761 protects vascular endothelium exposed to oxidized low density lipoproteins[J].Cell Mol Biol(Noisy-le-grand),2008,26(54 Suppl):OL1032-1042.
[22]孟庆楠,张皓楠,王益民.MTT法检测银杏叶注射液对人脑微血管内皮细胞增殖的影响[J].天津中医药大学学报,2011,30(1):36-40.
[23]徐倩,张艳军,刘洋,等.银杏内酯B作用于星形胶质细胞对神经干细胞趋化影响的体外研究[J].天津中医药,2010,27(5):421-424.