多目标遗传算法优选紫苏叶有效部位的提取工艺研究
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摘要
目的优选紫苏叶有效部位的提取工艺,确定最优提取条件。方法以乙醇浓度、溶媒倍数、提取次数及提取时间为考察因素,以总黄酮、总酚含量及浸膏得率为目标函数,采用正交试验优选紫苏叶有效部位的回流提取工艺,进一步运用多目标遗传算法优化正交试验结果。结果紫苏叶的最优提取工艺为:加药材19倍的量的75%乙醇回流提取3次,每次2.93 h;该提取条件下总黄酮、总酚含量及浸膏得率分别为27.24 mg/g、18.32 mg/g及17.07%,与预测结果相近。结论所得工艺合理、稳定可行,重现性好,可用于紫苏叶有效部位的提取。
Objective To optimize the extraction process of the effective parts from Folium Perillae,and determine the optimal extraction conditions. Methods Ethanol concentration,solvent ratio,extraction times and extraction time were regarded as the facters,and total flavonoids content,total phenols content and ratio of extraction were taken as the objective functions,the reflux extraction process of the effective parts from Folium Perillae was optimized by orthogonal test,further using multi-objective genetic algorithm(MOGA) to optimize the orthogonal test results. Results The optimal conditions of extraction process were as follows: adding 75% ethanol as 19 times as the amount of the medicinal material,to extract 3 times by reflowing and 2. 93 h each time. Under the conditions,total flavonoids content,total phenols content and ratio of extraction were 27. 24 mg/g,18. 32 mg/g,17. 07%,respectively,which were similar to the predicted results. Conclusion The optimized extraction process of Perillae Folium is reasonable,stable and feasible,and has a good reproducibility. Besides,it's suitable for extracting the effective parts from Folium Perillae.
Objective To optimize the extraction process of the effective parts from Folium Perillae,and determine the optimal extraction conditions. Methods Ethanol concentration,solvent ratio,extraction times and extraction time were regarded as the facters,and total flavonoids content,total phenols content and ratio of extraction were taken as the objective functions,the reflux extraction process of the effective parts from Folium Perillae was optimized by orthogonal test,further using multi-objective genetic algorithm(MOGA) to optimize the orthogonal test results. Results The optimal conditions of extraction process were as follows: adding 75% ethanol as 19 times as the amount of the medicinal material,to extract 3 times by reflowing and 2. 93 h each time. Under the conditions,total flavonoids content,total phenols content and ratio of extraction were 27. 24 mg/g,18. 32 mg/g,17. 07%,respectively,which were similar to the predicted results. Conclusion The optimized extraction process of Perillae Folium is reasonable,stable and feasible,and has a good reproducibility. Besides,it's suitable for extracting the effective parts from Folium Perillae.
引文
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[19]王雷,蔡劲草,李明.基于正交试验的遗传算法参数优化[J].南京师范大学学报(工程技术版),2016,16(2):81.
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[21]王振青,孙琦,回新更.紫苏叶饮片配合免煎颗粒治疗风寒感冒初起临床对比研究[J].河北医学,2016,22(4):657.
[22]陈燕芬,陈丽娟,谢文健.出膏率在控制膏方煎煮方面的正交研究[J].中国实验方剂学杂志,2010,16(11):11.
[2]国家药典委员会.中国药典,一部[S].北京:中国医药科技出版社,2015:339.
[3]杨慧,马培,林明宝,等.紫苏叶化学成分、抗炎作用及其作用机制研究进展[J].中国药理学与毒理学杂志,2017,31(3):279.
[4]Bae J S,Han M,Shin H S,et al.Perilla frutescens leaves extract ameliorates ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin[J].J Ethnopharmacol,2016,195:334.
[5]郭晓青,陈晓靓,杨春梅,等.紫苏叶活性成分及抗氧化性研究[J].食品与机械,2014,30(4):179.
[6]代沙.紫苏叶抗氧化物质提取、含量测定及抗氧化活性研究[D].四川农业大学硕士学位论文,2013.
[7]陈建欣,刘静,汪川苏.紫苏中黄酮的提取及测定研究[J].广州化工,2015,43(3):132.
[8]王柏强,何效平,曾芝兰.酶法协同超声提取紫苏叶总黄酮工艺条件优化[J].中华中医药学刊,2015,33(7):1741.
[9]马耿鑫,陈浩旋,刘娟,等.超声波辅助法提取紫苏叶酚类物质的研究[J].广州化工,2012,40(13):72.
[10]王根女,苏平,黄恺婷,等.响应面法优化微波提取紫苏叶多酚物质[J].中国食品学报,2009,9(5):87.
[11]殷明阳,刘素香,张铁军,等.复方中药提取工艺研究概况[J].中草药,2015,46(21):3279.
[12]牟会荣,陈昆,王晓岚,等.正交试验法与响应面法在桑叶黄酮提取工艺优化中的应用和比较[J].江苏科技大学学报(自然科学版),2016,30(1):88.
[13]刘鑫,宓嘉琪,吴巧凤.基于遗传算法优化黄连中盐酸小檗碱的提取工艺研究[J].中华中医药杂志,2013,28(12):3645.
[14]李莉,张赛,何强,等.响应面法在试验设计与优化中的应用[J].实验室研究与探索,2015,34(8):41.
[15]冯蓉洁,吕佩惠,盛振华,等.紫苏提取物抗氧化活性及酚性成分的研究[J].时珍国医国药,2009,20(5):1165.
[16]徐彦杰,师先锋,杨晓文,等.多目标遗传算法优化五味子提取工艺的研究[J].中国药学杂志,2012,22(9):669.
[17]徐冰婉,徐小雯,黄家卫.多目标遗传算法优化黄连的提取工艺[J].中成药,2015,37(4):768.
[18]朱文瑞,吴巧凤,宓嘉琪,等.基于遗传算法的土香薷总黄酮提取工艺优化研究[J].浙江中医药大学学报,2014,38(10):1207.
[19]王雷,蔡劲草,李明.基于正交试验的遗传算法参数优化[J].南京师范大学学报(工程技术版),2016,16(2):81.
[20]崔珊珊.遗传算法的一些改进及其应用[D].中国科学技术大学硕士学位论文,2010.
[21]王振青,孙琦,回新更.紫苏叶饮片配合免煎颗粒治疗风寒感冒初起临床对比研究[J].河北医学,2016,22(4):657.
[22]陈燕芬,陈丽娟,谢文健.出膏率在控制膏方煎煮方面的正交研究[J].中国实验方剂学杂志,2010,16(11):11.