走马胎中大叶紫金牛酚的分离鉴定与生物活性评价
|
摘要
为了解走马胎(Ardisia gigantifolia)的化学成分,从其乙酸乙酯提取物中分离鉴定出大叶紫金牛酚。体外活性评价结果表明,大叶紫金牛酚具有良好的抗炎、抗肿瘤活性以及一定的神经保护作用。这是首次对大叶紫金牛酚进行系统研究,为综合开发与利用走马胎提供科学依据。
In order to understand the chemical constituents of Ardisia gigantifolia, the gigantifolinol were isolated and identified from EtOAc extract. In vitro activity screening, gigantifolinol showed potent anti-inflammatory and anti-tumor activities, and moderate neuroprotection activities. This is the first systematic study of gigantifolinol,which might provide a scientific basis to the development and utilization of A. gigantifolia.
In order to understand the chemical constituents of Ardisia gigantifolia, the gigantifolinol were isolated and identified from EtOAc extract. In vitro activity screening, gigantifolinol showed potent anti-inflammatory and anti-tumor activities, and moderate neuroprotection activities. This is the first systematic study of gigantifolinol,which might provide a scientific basis to the development and utilization of A. gigantifolia.
引文
[1]LU W J,WANG X F,CHEN J Y,et al.Studies on the chemical constituents of Ardisia gigantifolia Stapf[J].W China J Pharm Sci,1990,5(3):136-138.doi:10.13375/j.cnki.wcjps.1990.03.005.卢文杰,王雪芬,陈家源,等.大叶紫金牛化学成分的研究[J].华西药学杂志,1990,5(3):136-138.doi:10.13375/j.cnki.wcjps.1990.03.005.
[2]GUAN Y F,SONG X,QIU M H,et al.Bioassay-guided isolation and structural modification of the anti-TB resorcinols from Ardisia gigantifolia[J].Chem Biol Drug Des,2016,88(2):293-301.doi:10.1111/cbdd.12756.
[3]DAI W B,DONG P P,MEI Q X.The research on the herbal of the Ardisia gigantifolia Stapf and its confusing products[J].J Chin Med Mat,2017,40(9):2220-2223.doi:10.13863/j.issn1001-4454.2017.09.051.戴卫波,董鹏鹏,梅全喜.走马胎及其混淆品的本草考证[J].中药材,2017,40(9):2220-2223.doi:10.13863/j.issn1001-4454.2017.09.051.
[4]Guangdong Food and Drug Administration.Standard of Guangdong Traditional Chinese Medicine[M].Guangzhou:Guangdong Science and Technology Press,2004:101-104.广东省食品药品监督管理局.广东省中药材标准[M].广州:广东科学技术出版社,2004:101-104.
[5]WEI R,FU J Q,XIE S M,et al.Research progress on the resources and applications of Ardisia gigantifolia[J].Guangdong For Sci Technol,2015,31(5):94-98.doi:10.3969/j.issn.1006-4427.2015.05.019.魏蓉,负建全,谢思明,等.走马胎资源与利用研究进展[J].广东林业科技,2015,31(5):94-98.doi:10.3969/j.issn.1006-4427.2015.05.019.
[6]LONG J C,XU C G,WEI G Y,et al.Progress in research of traditional Chinese medicine zoumatai(Ardisia gigantifolia)[J].Guid J Trad Chin Med Pharm,2017,23(21):75-79.doi:10.13862/j.cnki.cn43-1446/r.2017.21.024.龙杰超,徐传贵,韦贵元,等.中药走马胎研究进展[J].中医药导报,2017,23(21):75-79.doi:10.13862/j.cnki.cn43-1446/r.2017.21.024.
[7]YANG S J.37 treatment cases of gouty arthritis with Tongyao[J].JMed Pharm Chin Minorit,2007,2(2):47.doi:10.3969/j.issn.1006-6810.2007.02.052.杨圣金.侗药治疗痛风性关节炎37例[J].中国民族医药杂志,2007,2(2):47.doi:10.3969/j.issn.1006-6810.2007.02.052.
[8]TANG A P.The clinical observation on treatment of rheumatoid arthritis with the Ardisia gigantifolia Stapf[J].J Sichuan Trad Chin Med,2007,25(1):54-55.doi:10.3969/j.issn.1000-3649.2007.01.028.唐亚平.中药走马胎治疗类风湿关节炎的临床观察[J].四川中医,2007,25(1):54-55.doi:10.3969/j.issn.1000-3649.2007.01.028.
[9]LONG Z Z.48 treatment cases of hyperosteogeny with Guanguan mixture of Tongyao[J].J Med Pharm Chin Minorit,2007,13(11):13-14.doi:10.3969/j.issn.1006-6810.2007.11.012.龙智忠.侗药走观合剂治疗骨质增生症48例[J].中国民族医药杂志,2007,13(11):13-14.doi:10.3969/j.issn.1006-6810.2007.11.012.
[10]LONG K E,XIAO C W,LONG S,et al.The study on the treatment technology of fractures of skeletal fractures(6):Clinical observation on4118 treatmen cases of various bone fractures[J].J Med Pharm Chin Minorit,2013,19(5):22-24.doi:10.3969/j.issn.1006-6810.2013.05.015.龙开娥,萧成纹,龙驶,等.侗医骨伤骨折治疗技术研究(六)--侗医药治疗各类骨伤骨折4118例临床疗效观察[J].中国民族医药杂志,2013,19(5):22-24.doi:10.3969/j.issn.1006-6810.2013.05.015.
[11]PARK S H,PARK K H,OH M H,et al.Anti-oxidative and antiinflamematory activities of caffeoyl hemiterpene glycosides from Spiraea prunifolia[J].Phytochemistry,2013,96:430-436.doi:10.1016/j.phytochem.2013.09.017.
[12]ZHONG W M,CUI Z M,LIU Z K,et al.Three minor new compounds from the aerial parts of Leonurus japonicus[J].Chin Chem Lett,2015,26(8):1000-1003.doi:10.1016/j.cclet.2015.05.004.
[13]HEILMANN J,WASESCHA M R,SCHMIDT T J.The influence of glutathione and cysteine levels on the cytotoxicity of helenanolide type sesquiterpene lactones against KB cells[J].Bioorg Med Chem,2001,9(8):2189-2194.doi:10.1016/S0968-0896(01)00131-6.
[14]ZHANG C,ZHANG J,HUANG J,et al.Analysis based on stem cells kidney essence theory and mechanism of aging[J].Liaoning J Trad Chin Med,2014,41(9):1877-1879.doi:20.13192/j.issn.1000-1719.2014.09.035.张晨,张进,黄进,等.基于干细胞的“肾精”理论与衰老机制探析[J].辽宁中医杂志,2014,41(9):1877-1879.doi:20.13192/j.issn.1000-1719.2014.09.035.
[15]SHI A W,LU Q,HOU L L,et al.Mechanism study of Bushen Kangshuai decoction on senescence of Caenorhabditis elegans[J].Liaoning J Trad Chin Med,2013,40(1):52-54.史爱武,陆勤,侯莉莉,等.补肾抗衰汤影响秀丽隐杆线虫衰老的机制研究[J].辽宁中医杂志,2013,40(1):52-54.
[16]NALETOVA I,SATRIANO C,CURCI A,et al.Cytotoxic phenanthroline derivatives alter metallostasis and redox homeostasis in neuroblastoma cells[J].Oncotarget,2018,9(91):36289-36316.doi:10.18632/oncotarget.26346.
[17]PENG Y,XING S N,TANG H Y,et al.Influence of glucose transporter1 activity inhibition on neuroblastoma in vitro[J].Gene,2018,689:11-17.doi:10.1016/j.gene.2018.12.010.
[18]MENDELL A L,MACLUSKY N J.The testosterone metabolite 3α-androstanediol inhibits oxidative stress-induced ERK phosphorylation and neurotoxicity in SH-SY5Y cells through an MKP3/DUSP6-dependent mechanism[J].Neurosci Lett,2019,696:60-66.doi:10.1016/j.neulet.2018.12.012.
[19]GETACHEW B,CSOKA A B,ASCHNER M,et al.Nicotine protects against manganese and iron-induced toxicity in SH-SY5Y cells:Implication for Parkinson’s disease[J].Neurochem Int,2018,124:19-24.doi:10.1016/j.neuint.2018.12.003.
[2]GUAN Y F,SONG X,QIU M H,et al.Bioassay-guided isolation and structural modification of the anti-TB resorcinols from Ardisia gigantifolia[J].Chem Biol Drug Des,2016,88(2):293-301.doi:10.1111/cbdd.12756.
[3]DAI W B,DONG P P,MEI Q X.The research on the herbal of the Ardisia gigantifolia Stapf and its confusing products[J].J Chin Med Mat,2017,40(9):2220-2223.doi:10.13863/j.issn1001-4454.2017.09.051.戴卫波,董鹏鹏,梅全喜.走马胎及其混淆品的本草考证[J].中药材,2017,40(9):2220-2223.doi:10.13863/j.issn1001-4454.2017.09.051.
[4]Guangdong Food and Drug Administration.Standard of Guangdong Traditional Chinese Medicine[M].Guangzhou:Guangdong Science and Technology Press,2004:101-104.广东省食品药品监督管理局.广东省中药材标准[M].广州:广东科学技术出版社,2004:101-104.
[5]WEI R,FU J Q,XIE S M,et al.Research progress on the resources and applications of Ardisia gigantifolia[J].Guangdong For Sci Technol,2015,31(5):94-98.doi:10.3969/j.issn.1006-4427.2015.05.019.魏蓉,负建全,谢思明,等.走马胎资源与利用研究进展[J].广东林业科技,2015,31(5):94-98.doi:10.3969/j.issn.1006-4427.2015.05.019.
[6]LONG J C,XU C G,WEI G Y,et al.Progress in research of traditional Chinese medicine zoumatai(Ardisia gigantifolia)[J].Guid J Trad Chin Med Pharm,2017,23(21):75-79.doi:10.13862/j.cnki.cn43-1446/r.2017.21.024.龙杰超,徐传贵,韦贵元,等.中药走马胎研究进展[J].中医药导报,2017,23(21):75-79.doi:10.13862/j.cnki.cn43-1446/r.2017.21.024.
[7]YANG S J.37 treatment cases of gouty arthritis with Tongyao[J].JMed Pharm Chin Minorit,2007,2(2):47.doi:10.3969/j.issn.1006-6810.2007.02.052.杨圣金.侗药治疗痛风性关节炎37例[J].中国民族医药杂志,2007,2(2):47.doi:10.3969/j.issn.1006-6810.2007.02.052.
[8]TANG A P.The clinical observation on treatment of rheumatoid arthritis with the Ardisia gigantifolia Stapf[J].J Sichuan Trad Chin Med,2007,25(1):54-55.doi:10.3969/j.issn.1000-3649.2007.01.028.唐亚平.中药走马胎治疗类风湿关节炎的临床观察[J].四川中医,2007,25(1):54-55.doi:10.3969/j.issn.1000-3649.2007.01.028.
[9]LONG Z Z.48 treatment cases of hyperosteogeny with Guanguan mixture of Tongyao[J].J Med Pharm Chin Minorit,2007,13(11):13-14.doi:10.3969/j.issn.1006-6810.2007.11.012.龙智忠.侗药走观合剂治疗骨质增生症48例[J].中国民族医药杂志,2007,13(11):13-14.doi:10.3969/j.issn.1006-6810.2007.11.012.
[10]LONG K E,XIAO C W,LONG S,et al.The study on the treatment technology of fractures of skeletal fractures(6):Clinical observation on4118 treatmen cases of various bone fractures[J].J Med Pharm Chin Minorit,2013,19(5):22-24.doi:10.3969/j.issn.1006-6810.2013.05.015.龙开娥,萧成纹,龙驶,等.侗医骨伤骨折治疗技术研究(六)--侗医药治疗各类骨伤骨折4118例临床疗效观察[J].中国民族医药杂志,2013,19(5):22-24.doi:10.3969/j.issn.1006-6810.2013.05.015.
[11]PARK S H,PARK K H,OH M H,et al.Anti-oxidative and antiinflamematory activities of caffeoyl hemiterpene glycosides from Spiraea prunifolia[J].Phytochemistry,2013,96:430-436.doi:10.1016/j.phytochem.2013.09.017.
[12]ZHONG W M,CUI Z M,LIU Z K,et al.Three minor new compounds from the aerial parts of Leonurus japonicus[J].Chin Chem Lett,2015,26(8):1000-1003.doi:10.1016/j.cclet.2015.05.004.
[13]HEILMANN J,WASESCHA M R,SCHMIDT T J.The influence of glutathione and cysteine levels on the cytotoxicity of helenanolide type sesquiterpene lactones against KB cells[J].Bioorg Med Chem,2001,9(8):2189-2194.doi:10.1016/S0968-0896(01)00131-6.
[14]ZHANG C,ZHANG J,HUANG J,et al.Analysis based on stem cells kidney essence theory and mechanism of aging[J].Liaoning J Trad Chin Med,2014,41(9):1877-1879.doi:20.13192/j.issn.1000-1719.2014.09.035.张晨,张进,黄进,等.基于干细胞的“肾精”理论与衰老机制探析[J].辽宁中医杂志,2014,41(9):1877-1879.doi:20.13192/j.issn.1000-1719.2014.09.035.
[15]SHI A W,LU Q,HOU L L,et al.Mechanism study of Bushen Kangshuai decoction on senescence of Caenorhabditis elegans[J].Liaoning J Trad Chin Med,2013,40(1):52-54.史爱武,陆勤,侯莉莉,等.补肾抗衰汤影响秀丽隐杆线虫衰老的机制研究[J].辽宁中医杂志,2013,40(1):52-54.
[16]NALETOVA I,SATRIANO C,CURCI A,et al.Cytotoxic phenanthroline derivatives alter metallostasis and redox homeostasis in neuroblastoma cells[J].Oncotarget,2018,9(91):36289-36316.doi:10.18632/oncotarget.26346.
[17]PENG Y,XING S N,TANG H Y,et al.Influence of glucose transporter1 activity inhibition on neuroblastoma in vitro[J].Gene,2018,689:11-17.doi:10.1016/j.gene.2018.12.010.
[18]MENDELL A L,MACLUSKY N J.The testosterone metabolite 3α-androstanediol inhibits oxidative stress-induced ERK phosphorylation and neurotoxicity in SH-SY5Y cells through an MKP3/DUSP6-dependent mechanism[J].Neurosci Lett,2019,696:60-66.doi:10.1016/j.neulet.2018.12.012.
[19]GETACHEW B,CSOKA A B,ASCHNER M,et al.Nicotine protects against manganese and iron-induced toxicity in SH-SY5Y cells:Implication for Parkinson’s disease[J].Neurochem Int,2018,124:19-24.doi:10.1016/j.neuint.2018.12.003.