红外指纹图谱与双指标模型结合用于白芷品质分析
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摘要
利用共有峰率和变异峰率两个指标,鉴别了不同品质的白芷的红外指纹图谱,白芷样品按厂家各自聚为一类。并应用化学计量学中模式识别方法,即主成分分析和系统聚类分析法,对白芷样品共有特征高效液相色谱峰数据进行处理。主成分分析和系统聚类分析结果与双指标模型一致,川白芷与祁白芷共有峰率最高,达到83. 3%。运用红外指纹图谱与双指标模型结合的方法,可以对白芷进行简单快速准确鉴别,为白芷品质的评价提供了另一种思路。
Common and variation peaks were calculated as dual-index based on the infrared spectra( IR) of Angelicaedahuricae Radix with different qualities.Additionally,Recognition patterns of chemometrics were employed.Principle component analysis( PCA)and cluster analysis( CA) were applied in order to process data of mean characteristic high performance liquid chromatography peaks of Angelicaedahuricae Radix.Results of PCA and CA were nearly uniform,which illustrated samples could cluster to their own group in according to origin. The results demonstratedthatthe highest common peak rate between A1 and A9 reached83. 3%. The above method provide another rapid and accurate way for identification and quality control of Angelicaedahuricae Radix.
Common and variation peaks were calculated as dual-index based on the infrared spectra( IR) of Angelicaedahuricae Radix with different qualities.Additionally,Recognition patterns of chemometrics were employed.Principle component analysis( PCA)and cluster analysis( CA) were applied in order to process data of mean characteristic high performance liquid chromatography peaks of Angelicaedahuricae Radix.Results of PCA and CA were nearly uniform,which illustrated samples could cluster to their own group in according to origin. The results demonstratedthatthe highest common peak rate between A1 and A9 reached83. 3%. The above method provide another rapid and accurate way for identification and quality control of Angelicaedahuricae Radix.
引文
[1]刘晓昱,赖瑛.白芷香豆素储库型贴剂的研制及其体外评价[J].中草药,2018,49(2):313-317.
[2]吴媛媛,蒋桂华,马逾英,等.白芷的药理作用研究进展[J].时珍国医国药,2009,20(3):625-627.
[3]张庆芝,王开疆,刀莉芳.中药白芷的品种论述[J].云南中医学院学报,2000,23(2):22-24.
[4]Gong F,Liang Y Z,Fung Y S.Analysis of volatile components from Cortex cinnamomi with hyphenated chromatography and chemometric resolution[J]. J Pharmaceut Biomed,2003,34(5):1029-1047.
[5]Gong F,Liang Y Z,Xie P S.Information theory applied to chromatographic fingerprint of herbal medicine for quality control[J].J Chromatogr A,2003,1002(1):25-40.
[6]袁亚洲,倪永年.不同产地白芷药材高效液相色谱指纹图谱研究[J].分析科学学报,2009,25(1):26-30.
[7]舒翔,叶小春,陈军,等.高效液相色谱法同时测定白芷中欧前胡素和异欧前胡素的含量[J].中国医院药学杂志,2017,37(5):427.
[8]邓捷圆,高广慧,孙晓军.不同产地白芷药材HPLC指纹图谱的研究[J].药学服务与研究,2004,(4):335-338.
[9]王婷婷,陈晓辉,胡庆庆,等.白芷质量的HPLC指纹图谱评价方法[J].药学学报,2006,(8):747-751.
[10]单鸣秋,姚晓东,池玉梅,等.侧柏叶红外指纹图谱共有峰率和变异峰率双指标序列分析法[J].光谱学与光谱分析,2009,29(8):2092-2095.
[2]吴媛媛,蒋桂华,马逾英,等.白芷的药理作用研究进展[J].时珍国医国药,2009,20(3):625-627.
[3]张庆芝,王开疆,刀莉芳.中药白芷的品种论述[J].云南中医学院学报,2000,23(2):22-24.
[4]Gong F,Liang Y Z,Fung Y S.Analysis of volatile components from Cortex cinnamomi with hyphenated chromatography and chemometric resolution[J]. J Pharmaceut Biomed,2003,34(5):1029-1047.
[5]Gong F,Liang Y Z,Xie P S.Information theory applied to chromatographic fingerprint of herbal medicine for quality control[J].J Chromatogr A,2003,1002(1):25-40.
[6]袁亚洲,倪永年.不同产地白芷药材高效液相色谱指纹图谱研究[J].分析科学学报,2009,25(1):26-30.
[7]舒翔,叶小春,陈军,等.高效液相色谱法同时测定白芷中欧前胡素和异欧前胡素的含量[J].中国医院药学杂志,2017,37(5):427.
[8]邓捷圆,高广慧,孙晓军.不同产地白芷药材HPLC指纹图谱的研究[J].药学服务与研究,2004,(4):335-338.
[9]王婷婷,陈晓辉,胡庆庆,等.白芷质量的HPLC指纹图谱评价方法[J].药学学报,2006,(8):747-751.
[10]单鸣秋,姚晓东,池玉梅,等.侧柏叶红外指纹图谱共有峰率和变异峰率双指标序列分析法[J].光谱学与光谱分析,2009,29(8):2092-2095.