维生素C对亚硝酸盐胁迫下匙吻鲟仔鱼非特异性免疫及抗氧化能力的影响
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摘要
为初步探究水体中添加不同剂量维生素C对匙吻鲟内源营养期及混合营养期仔鱼抗亚硝酸盐胁迫能力的影响,采用生化方法测定不同条件下仔鱼溶菌酶(LZM)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)活性,丙二醛(MDA)含量和总抗氧化能力(T-AOC)在内源营养期及混合营养期的动态变化。结果显示:以卵黄为营养的内源营养期,LZM、AKP、ACP活性和T-AOC随维生素C质量浓度的增加呈现升高趋势,且90 mg/L和120 mg/L维生素C组显著高于对照组和胁迫组;以卵黄和外源饵料作营养的混合营养期,LZM、AKP、ACP活性随维生素C质量浓度的增加呈现"升高—降低"趋势。90 mg/L维生素C组LZM、AKP、ACP活性和T-AOC从内源营养期到混合营养期显著上升,上升率分别为71.8%、33.7%、48.4%和61.1%。MDA含量随维生素C质量浓度的增加而降低,且在同一取样时间下显著低于胁迫组,维生素C添加组MDA含量从内源营养期到混合营养期无显著变化。综上,在亚硝酸盐胁迫下,90 mg/L维生素C浸泡对匙吻鲟仔鱼内源营养期及混合营养期保护效果较好;亚硝酸盐胁迫可导致匙吻鲟内源营养期及混合营养期仔鱼机体的氧化损伤及抗氧化能力的不平衡,但不同质量浓度维生素C浸泡可以在一定程度上缓解亚硝酸盐胁迫的负面影响。
In order to investigate the effects of different concentrations vitamin C( Vc) on the non-specific immunity and antioxidant capacity of Polyodon spathula during endogenous and mixed nutrition under nitrite stress,the biochemical methods were employed to determine the activities of lysozyme( LZM),acid phosphatase( ACP),alkaline phosphatase( AKP),and the malondialdehyde( MDA) content and the total antioxidant capacity( T-AOC). The results suggested that during endogenous nutrition,the activities of LZM,AKP,ACP and T-AOC were increased in a concentration dependent manner,and the Vc 90 mg/L and 120 mg/L groups significantly increased( P < 0. 05) compared with the control and the stress groups; during the mixed nutrition,the activities of LZM,AKP and ACP increased first and then decreasedwith the rising of Vc concentration. In Vc 90 mg/L group,the T-AOC and the activities of LZM,AKP,ACP increased by 61. 1%,71. 8%,33. 7% and 48. 4% from endogenous to mixed nutrition. The MDA content was decreased with the rising of Vc concentration,and significantly lower than that of the stress group( P < 0. 05),but no significant differences between endogenous and mixed nutrition stages in all Vc groups. In conclusion,under nitrite stress,90 mg/L Vc soaking could protect the Polyodon spathula larvae during the endogenous and mixed nutrition,and the nitrite stress led to the imbalance of antioxidant capacity and oxidative damage,however,the Vc could alleviate the negative impact of nitrite stress.
In order to investigate the effects of different concentrations vitamin C( Vc) on the non-specific immunity and antioxidant capacity of Polyodon spathula during endogenous and mixed nutrition under nitrite stress,the biochemical methods were employed to determine the activities of lysozyme( LZM),acid phosphatase( ACP),alkaline phosphatase( AKP),and the malondialdehyde( MDA) content and the total antioxidant capacity( T-AOC). The results suggested that during endogenous nutrition,the activities of LZM,AKP,ACP and T-AOC were increased in a concentration dependent manner,and the Vc 90 mg/L and 120 mg/L groups significantly increased( P < 0. 05) compared with the control and the stress groups; during the mixed nutrition,the activities of LZM,AKP and ACP increased first and then decreasedwith the rising of Vc concentration. In Vc 90 mg/L group,the T-AOC and the activities of LZM,AKP,ACP increased by 61. 1%,71. 8%,33. 7% and 48. 4% from endogenous to mixed nutrition. The MDA content was decreased with the rising of Vc concentration,and significantly lower than that of the stress group( P < 0. 05),but no significant differences between endogenous and mixed nutrition stages in all Vc groups. In conclusion,under nitrite stress,90 mg/L Vc soaking could protect the Polyodon spathula larvae during the endogenous and mixed nutrition,and the nitrite stress led to the imbalance of antioxidant capacity and oxidative damage,however,the Vc could alleviate the negative impact of nitrite stress.
引文
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[19]陈家长,臧学磊,瞿建宏,等.温度胁迫下罗非鱼机体免疫力的变化及其对海豚链球菌易感性的影响[J].农业环境科学学报,2011,30(9):1896-1901.
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[22]Kalaimani N,Chakravarthy N,Shanmugham R,et al.Anti-oxidant status in embryonic,post-hatch and larval stages of Asian seabass(Lates calcarifer)[J].Fish Physiology&Biochemistry,2008,34(2):151-158.
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[24]王贞杰,叶保民,常青,等.饲料维生素C含量对圆斑星鲽幼鱼抗氨氮胁迫能力的影响[J].动物营养学报,2016,28(12):4054-4062.
[25]蒋左玉,姚俊杰,熊铧龙,等.葡萄糖、维生素C对普安银鲫卵黄囊仔鱼发育中LPL和HL活性的影响[J].水生生物学报,2014,39(1):73-79.
[2]王秀英.饵料维生素C对黑鲷仔鱼生长和体组织生化指标的影响[D].杭州:浙江大学,2004.
[3]姚俊杰,熊铧龙,蒋左玉,等.维生素C对普安银鲫早期发育中氧化损伤及总抗氧化能力的影响[J].动物学杂志,2015,50(4):581-590.
[4]葛立安.亚硝酸盐胁迫下维生素C和E对异育银鲫免疫功能的影响[D].武汉:华中农业大学,2008.
[5]高明辉.VC、VE对亚硝酸盐胁迫下异育银鲫血液指标及抗氧化能力的影响[D].武汉:华中农业大学,2008.
[6]郝甜甜,王丽丽,王际英,等.维生素C对急性低温胁迫下珍珠龙胆石斑鱼HPI轴及生理生化的调控[J].水产学报,2017,41(3):428-437.
[7]周显青,牛翠娟,孙儒泳.维生素C和酸应激对中华鳖幼鳖血清补体C3和C4含量的影响[J].动物学报,2003,49(6):769-774.
[8]王建梅.弧菌和盐度胁迫下饵料中添加抗氧化物质对虾体的影响[D].保定:河北大学,2003.
[9]胡毅,黄云,文华,等.维生素C对青鱼幼鱼生长、免疫及抗氨氮胁迫能力的影响[J].水产学报,2013,37(4):565-573.
[10]王贞杰,叶保民,常青,等.饲料维生素C含量对圆斑星鲽幼鱼抗氨氮胁迫能力的影响[J].动物营养学报,2016,28(12):4054-4062.
[11]徐维娜,刘文斌,邵仙萍,等.维生素C对异育银鲫原代肝脏细胞活性及抗敌百虫氧化胁迫的影响[J].水产学报,2011,35(12):1849-1856.
[12]刘晓玲,周忠良,陈立侨,等.维生素C、E对镉致毒后中华绒螯蟹抗氧化系统酶活性的影响[J].海洋科学,2006,30(1):39-43.
[13]谭树华,何典翼,严芳,等.亚硝酸钠对鲫鱼肝脏丙二醛含量和总抗氧化能力的影响[J].农业环境科学学报,2005,24(S1):21-24.
[14]吕晓燕,李嘉尧,方燕,等.亚硝酸盐对红螯光壳螯虾不同组织免疫相关酶活性及超微结构的影响[J].水产学报,2010,34(12):1812-1820.
[15]Zhang L,Xiong D M,Li B,et al.Toxicity of ammonia and nitrite to yellow catfish(Pelteobagrus fulvidraco)[J].Journal of Applied Ichthyology,2012,28(1):82-86.
[16]冼健安,张秀霞,郭慧,等.亚硝酸盐胁迫对罗氏沼虾血细胞及其抗氧化酶活力的影响[J].生物安全学报,2016,25(4):300-307.
[17]Kanlis G,Suzuki Y,Tauchi M,et al.Immunoglobulin in oocytes,fertilized eggs,and yolk sac larvae of Red Sea Bream[J].Fisheries Science,2008,61(5):787-790.
[18]陈家长,臧学磊,胡庚东,等.氨氮胁迫下罗非鱼机体免疫力的变化及其对海豚链球菌易感性的影响[J].生态环境学报,2011,20(4):629-634.
[19]陈家长,臧学磊,瞿建宏,等.温度胁迫下罗非鱼机体免疫力的变化及其对海豚链球菌易感性的影响[J].农业环境科学学报,2011,30(9):1896-1901.
[20]Kroupova H,Machova J,Svobodova Z.Nitrite influence on fish:A review[J].VeterinárníMedicína,2005,50(11):461-471.
[21]陈家长,臧学磊,孟顺龙,等.亚硝酸盐氮胁迫对罗非鱼血清非特异性免疫酶活性的影响[J].生态环境学报,2012,21(5):897-901.
[22]Kalaimani N,Chakravarthy N,Shanmugham R,et al.Anti-oxidant status in embryonic,post-hatch and larval stages of Asian seabass(Lates calcarifer)[J].Fish Physiology&Biochemistry,2008,34(2):151-158.
[23]彭俊.几种环境因子对尼罗罗非鱼受精、孵化及幼鱼生长的联合效应研究[D].湛江:广东海洋大学,2011.
[24]王贞杰,叶保民,常青,等.饲料维生素C含量对圆斑星鲽幼鱼抗氨氮胁迫能力的影响[J].动物营养学报,2016,28(12):4054-4062.
[25]蒋左玉,姚俊杰,熊铧龙,等.葡萄糖、维生素C对普安银鲫卵黄囊仔鱼发育中LPL和HL活性的影响[J].水生生物学报,2014,39(1):73-79.