红豆蔻、大高良姜乙酸乙酯部位对胃溃疡寒证大鼠胃组织GAS、MOT、EGF、VEGF、6-keto-PGF1α的影响
|
摘要
目的:观察山姜属中药红豆蔻与大高良姜乙酸乙酯部位对胃溃疡寒证模型大鼠胃泌素(GAS)、胃动素(MOT)、表皮生长因子(EGF)、血管内皮生长因子(VEGF)、6-酮-前列腺素F1α(6-keto-PGF1α)的影响,以期探讨其对胃溃疡寒证大鼠温胃散寒的作用机制。方法:70只大鼠随机分为空白组、模型组、阳性对照组、红豆蔻与大高良姜乙酸乙酯高低剂量组(分别称为红乙高、低组;大乙高、低组),每组10只。采用15%冰乙酸和4℃知母水煎液复制胃溃疡寒证模型。造模成功后,4个受试药物组分别灌胃红豆蔻与大高良姜乙酸乙酯高、低剂量药物,测定大鼠胃组织GAS、MOT、EGF、VEGF、6-keto-PGF1α的含量。结果:与模型组比较,各给药组GAS、MOT含量显著降低(P<0.01);EGF、VEGF、6-keto-PGF1α含量显著升高(P<0.01,P<0.05,除红乙低组6-keto-PGF1α外);与大乙高组比较,红乙高组可显著降低GAS、MOT含量(P<0.01),显著升高VEGF含量(P<0.05)。结论:红豆蔻与大高良姜乙酸乙酯部位可通过降低GAS、MOT含量,升高EGF、VEGF、6-ketoPGF1α含量而发挥其温胃散寒的功效及胃溃疡保护作用。
Objective: To study the effects and mechanisms of ethyl acetate parts of Alpinia galanga Will. and Alpinia galangal(L.) Will on GAS, MOT, EGF, VEGF and 6-keto-PGF1α contents in gastro-tissue of gastric ulcer rats with cold syndrome, and to research the pharmacodynamic mechanism of them. Methods: Seventy rats were randomly divided into 7 groups: blank group, model group, positive control group, high and low dose ethyl acetate parts of Alpinia galanga Will. groups, high and low dose ethyl acetate parts of Alpinia galangal(L.) Will. groups, 10 mice each group. Gastric ulcer rats with cold syndrome models were established by 15%glacial acetic acid and 4℃ water decoction of Rhizoma Anemarrhenae. Four TCM treated groups were administrated with corresponding medicines. GAS, MOT, EGF, VEGF, 6-keto-PGF of stomach tissues were determined. Results: Compared to the model group, each dosage group significantly reduced the concentration of stomach GAS, MOT(P<0.01) and remarkably increased EGF, VEGF, 6-keto-PGF1α(P<0.01, P<0.05), except the low does ethyl acetate parts of Alpinia galanga Will.'s 6-keto-PGF1α. Compared with the high dose group of the ethyl acetate parts of Alpinia galangal(L.)Will., the high doses of the ethyl acetate parts of Alpinia galanga Will. significantly reduced the concentration of stomach GAS, MOT(P<0.01) and increased VEGF remarkably(P<0.05). Conclusion: The ethyl acetate parts of Alpinia galanga Will. and Alpinia galangal(L.)Will. has good improving effect by decreasing the content of GAS, MOT, and increasing the content of EGF, VEGF, 6-keto-PGF1α. It is indicated that the ethyl acetate parts of Alpinia galanga Will. and Alpinia galangal(L.) Will. are the main active parts and has close relevance with the function of warming stomach for dispelling cold and protective effect for gastric ulcer.
Objective: To study the effects and mechanisms of ethyl acetate parts of Alpinia galanga Will. and Alpinia galangal(L.) Will on GAS, MOT, EGF, VEGF and 6-keto-PGF1α contents in gastro-tissue of gastric ulcer rats with cold syndrome, and to research the pharmacodynamic mechanism of them. Methods: Seventy rats were randomly divided into 7 groups: blank group, model group, positive control group, high and low dose ethyl acetate parts of Alpinia galanga Will. groups, high and low dose ethyl acetate parts of Alpinia galangal(L.) Will. groups, 10 mice each group. Gastric ulcer rats with cold syndrome models were established by 15%glacial acetic acid and 4℃ water decoction of Rhizoma Anemarrhenae. Four TCM treated groups were administrated with corresponding medicines. GAS, MOT, EGF, VEGF, 6-keto-PGF of stomach tissues were determined. Results: Compared to the model group, each dosage group significantly reduced the concentration of stomach GAS, MOT(P<0.01) and remarkably increased EGF, VEGF, 6-keto-PGF1α(P<0.01, P<0.05), except the low does ethyl acetate parts of Alpinia galanga Will.'s 6-keto-PGF1α. Compared with the high dose group of the ethyl acetate parts of Alpinia galangal(L.)Will., the high doses of the ethyl acetate parts of Alpinia galanga Will. significantly reduced the concentration of stomach GAS, MOT(P<0.01) and increased VEGF remarkably(P<0.05). Conclusion: The ethyl acetate parts of Alpinia galanga Will. and Alpinia galangal(L.)Will. has good improving effect by decreasing the content of GAS, MOT, and increasing the content of EGF, VEGF, 6-keto-PGF1α. It is indicated that the ethyl acetate parts of Alpinia galanga Will. and Alpinia galangal(L.) Will. are the main active parts and has close relevance with the function of warming stomach for dispelling cold and protective effect for gastric ulcer.
引文
[1]国家药典委员会.中华人民共和国药典.北京:中国医药出版社,2015:153
[2]国家中医药管理局《中华本草》编委会.中华本草.上海:上海科学技术出版社,1999:7741-7745
[3]陈毓亨,童玉懿,段华,等.我国姜科药用植物研究——Ⅲ.红豆蔻的原植物和生药鉴别.中药材科技,1984(2):24-31
[4]中华人民共和国卫生部药典委员会.中华人民共和国药典.北京:人民卫生出版社,化学工业出版社,2000:118
[5]中国医学科学院.中药志.北京:人民卫生出版社,1984:7
[6]柳俊辉.5味山姜属中药对胃溃疡寒证大鼠温胃散寒药效及作用机制研究.广州:广州中医药大学,2016
[7]秦华珍,罗君,翁铭钻,等.5味山姜属中药对胃溃疡寒证大鼠环核苷酸水平的影响.世界中医药,2016,11(11):2231-2235
[8]张吉仲,李利民,黄利,等.半夏泻心汤及其拆方对脾虚大鼠胃泌素和生长抑素的影响.中药药理与临床,2013,29(1):15-17
[9]马刚,戴伟杰,严伟,等.盐酸依托必利联合α-硫辛酸对糖尿病胃轻瘫患者的疗效及对胃泌素、胃动素影响.世界华人消化杂志,2015,23(5):782-787
[10]李冀,柴剑波,李胜志,等.寒、热方剂对消炎痛型胃溃疡寒、热证模型大鼠血清GAS,MOT含量的影响.中国实验方剂学杂志,2011,17(20):170-172
[11]刘梅洁,李鸿泓,赵宏艳,等.脾虚、肾虚级脾肾两虚证大鼠外周血中OPG、RANKL、VIP、MTL及GAS不同表达特点.中华中医药杂志,2015,30(10):3466-3469
[12]曲怡,才丽平,郑洪新,等.中药消痈溃得康对乙酸胃溃疡模型大鼠PGE2及EGF含量的影响.中华中医药杂志,2011,26(10):2255-2257
[13]王志红,鲍德明,赵敏,等.EGF、VEGF在老年人胃溃疡中的表达及其与溃疡愈合的关系.中华全科医学,2012,10(2):187-189
[14]李成军,王丽萍,夏立丁,等.大鼠乙酸胃溃疡愈合过程中EGF和VEGF对胃黏膜组织形态的影响.医学研究杂志,2010,39(8):56-59
[15]安贺军,王新月,于玫,等.益气活血解毒法对溃疡性结肠炎复发患者血浆血栓烷B2和6-酮-前列腺素F1α水平及比值的影响.中国中西医结合消化杂志,2009,17(6):351-353
[16]李冀,刘波,李莆媛.香砂六君子汤对脾虚胃溃疡证病结合模型大鼠的影响.辽宁中医杂志,2008,35(4):505-506
[2]国家中医药管理局《中华本草》编委会.中华本草.上海:上海科学技术出版社,1999:7741-7745
[3]陈毓亨,童玉懿,段华,等.我国姜科药用植物研究——Ⅲ.红豆蔻的原植物和生药鉴别.中药材科技,1984(2):24-31
[4]中华人民共和国卫生部药典委员会.中华人民共和国药典.北京:人民卫生出版社,化学工业出版社,2000:118
[5]中国医学科学院.中药志.北京:人民卫生出版社,1984:7
[6]柳俊辉.5味山姜属中药对胃溃疡寒证大鼠温胃散寒药效及作用机制研究.广州:广州中医药大学,2016
[7]秦华珍,罗君,翁铭钻,等.5味山姜属中药对胃溃疡寒证大鼠环核苷酸水平的影响.世界中医药,2016,11(11):2231-2235
[8]张吉仲,李利民,黄利,等.半夏泻心汤及其拆方对脾虚大鼠胃泌素和生长抑素的影响.中药药理与临床,2013,29(1):15-17
[9]马刚,戴伟杰,严伟,等.盐酸依托必利联合α-硫辛酸对糖尿病胃轻瘫患者的疗效及对胃泌素、胃动素影响.世界华人消化杂志,2015,23(5):782-787
[10]李冀,柴剑波,李胜志,等.寒、热方剂对消炎痛型胃溃疡寒、热证模型大鼠血清GAS,MOT含量的影响.中国实验方剂学杂志,2011,17(20):170-172
[11]刘梅洁,李鸿泓,赵宏艳,等.脾虚、肾虚级脾肾两虚证大鼠外周血中OPG、RANKL、VIP、MTL及GAS不同表达特点.中华中医药杂志,2015,30(10):3466-3469
[12]曲怡,才丽平,郑洪新,等.中药消痈溃得康对乙酸胃溃疡模型大鼠PGE2及EGF含量的影响.中华中医药杂志,2011,26(10):2255-2257
[13]王志红,鲍德明,赵敏,等.EGF、VEGF在老年人胃溃疡中的表达及其与溃疡愈合的关系.中华全科医学,2012,10(2):187-189
[14]李成军,王丽萍,夏立丁,等.大鼠乙酸胃溃疡愈合过程中EGF和VEGF对胃黏膜组织形态的影响.医学研究杂志,2010,39(8):56-59
[15]安贺军,王新月,于玫,等.益气活血解毒法对溃疡性结肠炎复发患者血浆血栓烷B2和6-酮-前列腺素F1α水平及比值的影响.中国中西医结合消化杂志,2009,17(6):351-353
[16]李冀,刘波,李莆媛.香砂六君子汤对脾虚胃溃疡证病结合模型大鼠的影响.辽宁中医杂志,2008,35(4):505-506