NaCl协同蒸汽爆破对玉米芯半纤维素分离及纤维素酶解效率的影响
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摘要
研究了NaCl预浸协同蒸汽爆破预处理对玉米芯半纤维素分离和纤维素残渣酶水解的影响。结果表明,NaCl能够显著提高蒸汽爆破对玉米芯半纤维素的分离作用。当预浸NaCl溶液浓度为0.3 mol·L~(-1)时,爆破后固相残渣中半纤维素的残留量由单独蒸汽爆破的54.6 g·kg~(-1)玉米芯降低为34.5 g·kg~(-1)玉米芯,利用水浸提得到的木糖和木聚糖计算玉米芯木聚糖回收率达到86.53%,显著高于单独蒸汽爆破的59.95%。固相残渣纤维素酶水解转化率和玉米芯纤维素回收率分别为80.58%和74.57%,均高于对照组的73.01%和71.05%,但未达到差异显著水平。由此可见,NaCl协同蒸汽爆破能够显著增强蒸汽爆破对玉米芯的解构作用,提高木聚糖的回收率,但对纤维素酶水解作用不显著。
In this study,synergistic effects of NaCl with steam explosion pretreatment on hemicellulose separation and enzymatic hydrolysis of cellulose of corn cob was analyzed. The results showed that NaCl could promote the degradation of hemicellulose in corn cob facilitated by steam explosion. When the concentration of NaCl solution was 0. 3 mol·L~(-1),the hemicellulose in solid residue of corn cob was reduced from 54. 6 g·kg~(-1) to 34. 5 g·kg~(-1) after steam explosion. The recovery rate of xylan was86. 53%,which was significantly higher than 59. 95% by single steam explosion. The conversion rate and recovery rate of cellulose was 80. 58% and 74. 57%,which was higher than those pretreated by steam explosion alone( 73. 01% and 71. 05%),respectively. It is showed that NaCl-steam explosion could significantly enhance the destructing effect on corn cob and improve the recovery of xylan.
In this study,synergistic effects of NaCl with steam explosion pretreatment on hemicellulose separation and enzymatic hydrolysis of cellulose of corn cob was analyzed. The results showed that NaCl could promote the degradation of hemicellulose in corn cob facilitated by steam explosion. When the concentration of NaCl solution was 0. 3 mol·L~(-1),the hemicellulose in solid residue of corn cob was reduced from 54. 6 g·kg~(-1) to 34. 5 g·kg~(-1) after steam explosion. The recovery rate of xylan was86. 53%,which was significantly higher than 59. 95% by single steam explosion. The conversion rate and recovery rate of cellulose was 80. 58% and 74. 57%,which was higher than those pretreated by steam explosion alone( 73. 01% and 71. 05%),respectively. It is showed that NaCl-steam explosion could significantly enhance the destructing effect on corn cob and improve the recovery of xylan.
引文
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[2]中国国家统计局.中国统计年鉴[M].北京:中国统计出版社,2017.
[3]GUO X,ZHANG R,LI Z,et al.A novel pathway construction in Candida tropicalis for direct xylitol conversion from corncob xylan[J].Bioresource Technology,2013,128:547-552.
[4]LUO W,WANG J,LIU X,et al.A facile and efficient pretreatment of corncob for bioproduction of butanol[J].Bioresource technology,2013,140(3):86-89.
[5]许凤,张逊,周霞,等.农林生物质预处理过程中细胞壁主要组分溶解机理研究进展[J].林业工程学报,2016,1(4):1-9.
[6]CARVALHO A F,OLIVA N P,SILVA D F,et al.Xylo-oligosaccharides from lignocellulosic materials:Chemical structure,health benefits and production by chemical and enzymatic hydrolysis[J].Food Research International,2013,51(1):75-85.
[7]LI H,DENG A,REN J,et al.Catalytic hydrothermal pretreatment of corncob into xylose and furfural via solid acid catalyst[J].Bioresource Technology,2014,158(4):313-320.
[8]于政道.瞬时弹射式蒸汽爆破技术及其影响生物质转化机理的试验研究[D].郑州:河南农业大学,2015.
[9]CHEN H Z,LIU Z H.Steam explosion and its combinatorial pretreatment refining technology of plant biomass to bio-based products.[J].Biotechnology Journal,2015,10(6):866-885.
[10]SHEN X J,WANG B,HUANG P L,et al.Effects of aluminum chloride-catalyzed hydrothermal pretreatment on the structural characteristics of lignin and enzymatic hydrolysis[J].Bioresource Technology,2016,206:57-64.
[11]SUN Y,LU X,ZHANG S,et al.Kinetic study for Fe(NO3)3catalyzed hemicellulose hydrolysis of different corn stover silages[J].Bioresource Technology,2011,102(3):2936-2942.
[12]LI L,SUN J,CAI C,et al.Corn stover pretreatment by inorganic salts and its effects on hemicellulose and cellulose degradation[J].Bioresource Technology,2009,100(23):5865-5871.
[13]KANG K E,PARK D H,JEONG G T.Effects of NH4Cl and Mg Cl2on pretreatment and xylan hydrolysis of miscanthus straw[J].Carbohydrate Polymers,2013,92(2):1321-1326.
[14]贾桂娟,杜晓霞,董元锋.无机盐预处理对棉秆酶水解的影响[J].中华纸业,2013,34(20):29-34.
[15]王风芹,肖元玺,仝银杏,等.初始含水量对蒸汽爆破实现玉米芯半纤维素分离与纤维素酶水解的影响[J].河南农业大学学报,2017,51(6):834-838.
[16]张红漫,郑荣平,陈敬文,等.NREL法测定木质纤维素原料组分的含量[J].分析试验室,2010,29(11):15-18.
[17]WANG W,LING H,ZHAO H.Steam explosion pretreatment of corn straw on xylose recovery and xylitol production using hydrolysate without detoxification[J].Process Biochemistry,2015,50(10):1623-1628.
[18]XU J,THOMSEN M H,THOMSEN A B.Investigation of acetic acid-catalyzed hydrothermal pretreatment on corn stover[J].Applied Microbiology and Biotechnology,2010,86(2):509-516.
[19]JING Z,ZHANG H M,ZHENG R P,et al.The enhancement of pretreatment and enzymatic hydrolysis of corn stover by Fe SO4pretreatment[J].Biochemical Engineering Journal,2011,56(3):158-164.
[20]KAMIREDDY S R,LI J,TUCKER M,et al.Effects and mechanism of metal chloride salts on pretreatment and enzymatic digestibility of corn stover[J].Industrial&Engineering Chemistry Research,2013,52(5):1775-1782.
[21]LIU H,ZHU J Y.Eliminating inhibition of enzymatic hydrolysis by lignosulfonate in unwashed sulfite-pretreated aspen using metal salts.[J].Bioresource Technology,2010,101(23):9120-9127.
[22]AKIMKULOVA A,ZHOU Y,ZHAO X,et al.Improving the enzymatic hydrolysis of dilute acid pretreated wheat straw by metal ion blocking of non-productive cellulase adsorption on lignin[J].Bioresource Technology,2016,208:110-116.
[23]VASCONCELLOS V M,TARDIOLI P W,GIORDANO R L,et al.Addition of metal ions to a(hemi)cellulolytic enzymatic cocktail produced in-house improves its activity,thermostability,and efficiency in the saccharification of pretreated sugarcane bagasse[J].Nature Biotechnology,2016,33(3):331-337.