粉丝加工废水发酵生产农用γ-聚谷氨酸工艺优化
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摘要
利用粉丝加工过程中产生的废水为主要原料,以枯草芽孢杆菌发酵生产的γ-聚谷氨酸产量为考察目标,采用单因子试验和正交试验,优化碳源、氮源、无机盐、底物、pH、发酵时间等因素,研究不同发酵培养基和发酵条件对γ-聚谷氨酸产量的影响。结果表明,粉丝加工废水中添加葡萄糖2%,磷酸氢二钾0.3%,谷氨酸钠5%,在37℃发酵培养26 h,γ-聚谷氨酸含量最高达到53.7 g/L。实现了粉丝生产废水资源化利用,同时极大地降低了农用γ-聚谷氨酸的生产成本。
In order to research the effects of different fermentation media and fermentation conditions in vermicelli wastewater producing γ-polyglutamic acid by Bacillus subtilis, single factor test and orthogonal test was adopted,factors such as carbon source, nitrogen source, inorganic salt, substrate and pH were optimized. The results showed that the best fermentation media is 2% glucose, 0.3% dipotassium hydrogen phosphate, 5% sodium glutamate, the content of γ-polyglutamic acid is higher than 53.7 g/L of 26 h fermentation time at 37 ℃. This study realized the resource utilization of vermicelli processing wastewater, and greatly reduce the production costs of agriculturalγ-polyglutamic acid.
In order to research the effects of different fermentation media and fermentation conditions in vermicelli wastewater producing γ-polyglutamic acid by Bacillus subtilis, single factor test and orthogonal test was adopted,factors such as carbon source, nitrogen source, inorganic salt, substrate and pH were optimized. The results showed that the best fermentation media is 2% glucose, 0.3% dipotassium hydrogen phosphate, 5% sodium glutamate, the content of γ-polyglutamic acid is higher than 53.7 g/L of 26 h fermentation time at 37 ℃. This study realized the resource utilization of vermicelli processing wastewater, and greatly reduce the production costs of agriculturalγ-polyglutamic acid.
引文
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[2]陈咏竹,孙启玲.γ-多聚谷氨酸的性质、发酵生产及其应用[J].微生物学通报,2004,31(1):122-126.
[3]王萌,许孝瑞.γ-聚谷氨酸在农业应用中的研究进展[J].黑龙江农业科学,2014(10):161-163.
[4]史文娟,梁嘉平,陶汪海,等.添加γ-聚谷氨酸减少土壤水分深层渗漏提高持水能力[J].农业工程学报,2015,31(23):94-97.
[5]万俊杰,邓毛程.γ-聚谷氨酸絮凝剂培养条件及处理啤酒废水研究[J].环境科学与技术,2010,33(4):157-159.
[6]孙廷丽,施庆珊,欧阳友生,等.聚谷氨酸在医药领域的应用[J].生物技术进展,2012,2(1):34-38.
[7]王国良,关阳,张秀荣,等.γ-聚谷氨酸在食品中的功能性研究进展[J].食品工业,2013,34(10):210-213.
[8]张天娇,刘霞,邓观杰,等.γ-聚谷氨酸体外抗氧化性及抑制透明质酸酶活性的研究[J].食品与药品,2017,19(3):153-157.
[9]Ivanovics Q,Erdos L.Ein Beitragzum Wesen der Kapselsubstanz des Milzbrand--bazillus[J].Z Immuntatsforsch,1937,90:5-19.
[10]Ivanovics G,Bruckncr V.Chemische und immunologische studien uber den mechanismus der milzbrandinfecktion und immunitat die chemische struktur der kapselsubstanz des milzbrandbazillus und der serolish identischen spezifichen substanz des Bacillus mesentericus[J].Zeitschrift Für Immunit?tsforschung Immunobiolo gy,1937,90(8):304-318.
[11]Bovamick M.The Fomation of Extracellular D-Glutamic Acid PolyPeptide by Bacillus subtilis[J].J Biol Chem,1942,145:415-424.
[12]Hoppensack A,Oppermann-Sanio F B,Steinbüchel A.Conversion of the Nitrogen Content in Liquid Manure into Biomass and PolyGlutamic Acid by a Newly Isolated Strain of Bacillus Licheniformis[J].Microbiol.Lett,2003,218(1):39-45.
[13]Chen X,Chen S,Sun M,et al.High Yield of Poly-γ-Glutamic Acid from Bacillus subtilis by Solid-State Fermentation using Swine Manure as the Basis of a Solid Substrate[J].Bioresour.Technol,2005,96(17):1872-1879.
[14]DufosséL,De La Broise D,Guerard F.Evaluation of Nitrogenous Substrates such as Peptones from Fish:a New Method Based on Gompertz Modeling of Microbial Growth[J].Curr.Microbiol.2001,42(1):32-38.
[15]Dan Zhang,Xiaohai Feng,Zhe Zhou,et al.Economical Production of Poly(γ-Glutamic acid)using Untreated Cane Molasses and Monosodium Glutamate Waste Liquor by Bacillus subtilis NX-2[J].Bioresource Technology,2012(114):583-588.
[16]Chao Zhang,Daoji Wu,Jie Jia,et al.Fishmeal Wastewater as A LowCost Source forγ-Polyglutamic Acid Production Using Bacillus subtilis[J].Waste Biomass Valor,2017(20):1-7.
[17]惠明,田青,张彬,等.pH值对聚谷氨酸发酵液黏度及聚合物结构的影响[J].食品与发酵工业,2006,32(10):5-8.