摘要
目的探讨超声辐射力促进靶向微泡粘附提高肾缺血再灌注(IR)损伤检测的敏感性及可行性。方法制作小鼠肾缺血再灌注模型及正常对照组,并根据再灌注后1、3、6、12、24 h分别分为IRlh、IR3 h、IR6 h、IR12 h、IR24 h组。各组再根据有无超声辐射力作用随机分为单击靶向微泡(MBICAM)组和靶向微泡+超声辐射力组(MBICAM+USRF)组。结果 MBICAM组和MB_(ICAM+USRF)组在对照组肾脏均未见明显显影增强,肾脏声强度(VI)值无显著差异(6.47±0.42对6.45±0.62,P=0.923)。肾脏IR损伤不同时间窗下均可见显影增强,并且随着时间的推移,显影强度逐渐增高。MB_(ICAM+USRF)组缺血再灌注各时间窗肾脏VI值较MBICAM组显著增大,两者之间均存在显著性差异(P均=0.000)。IR12 h和IR24 h VI值之间无显著差异(P=1.000),其余各组之间均存在显著差异(P<0.05)。结论应用超声辐射力联合携抗细胞间黏附分子1单抗靶向微泡可提高小鼠肾脏缺血再灌注损伤检测敏感性,可用于早期评价微血管炎症或相关的血管内皮反应
Objective To study the sensitivity and feasibility of detecting microvascular inflammation in renal ischemiareperfusion injury using microbubbles(MB) targeted to the intercellular adhesion molecule ICAM-1 and ultrasound radiation force(USRF). Methods Mouse models of kidney ischemia-reperfusion were randomized into 5 groups with reperfusion time of 1,3,6,12,and 24 h(IRlh,IR3 h,IR6 h,IR12 h,and IR24 h group,respectively). Each group was subdivided into targeted MB group(MBICAMgroup) and targeted MB+USRF group(MB_(ICAM+USRF) group). Kidney enhancement and the video intensity(VI) of the kidneys were compared among the groups. Results In normal mice in either MBICAMor MB_(ICAM+USRF) group,no obvious enhancement of the kidney or significant increase in VI of the kidneys was observed(P=0.923). The kidneys were enhanced in all the mice with renal ischemia-reperfusion injury,and with the passage of time,the enhancement increased progressively. VI in the kidneys of mice with renal ischemia-reperfusion injury in MB_(ICAM+USRF) group increased more significantly compared with the MBICAMgroup. Significant difference in the VI was noted among the groups with different perfusion time but not between IR12 h and IR24 h groups. Conclusion Microbubbles targeted to ICAM-1 combined with USRF can effectively evaluate renal ischemia-reperfusion injury in mice and can be used for early evaluation of microvascular inflammation and other endothelial responses.
引文
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