长治移民与非移民血浆叶酸及代谢相关基因多态性比较
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摘要
目的探讨山西省长治市移民与非移民人群血浆叶酸含量、亚甲基叶酸还原酶MTHFRC677T和甲硫氨酸合成酶MSA2756G位点多态性分布特点,为进一步研究其与疾病的相关性提供依据。方法于2014年5月—2015年6月采用酶联免疫吸附试验(ELISA)对80名长治林州移民和108名长治本地健康人群测定血浆中叶酸含量。同时采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)分析长治市人群MTHFR677位点和MS2756位点基因多态性。结果移民组与非移民组人群叶酸含量差异有统计学意义(P=0.006)。移民组内不同性别、年龄和是否饮酒的人群之间叶酸水平差异有统计学意义(P<0.05);非移民组组内不同血型的人群间差异有统计学意义(P<0.05)。2组组间比较结果表明:女性、>60岁、体质指数(BMI)<18.5 kg/m2、不吸烟和不饮酒的人群叶酸水平差异有统计学意义,MTHFR、MS的基因型和等位基因频率比较差异有统计学意义(P<0.05)。结论在不同特征的长治移民与非移民人群中血浆叶酸含量不同,2组人群MTHFR和MS基因多态性分布存在明显差异。
Objective To investigate the content of plasma folic acid(FA) and the gene polymorphisms of methylenetetrahydrofolate reductase(MTHFR C677 T) and methionine synthase(MS A2756 G) among migrants and nonmigrants in Changzhi city of Shanxi province, and to provide evidences for further research. Methods We recruited 80 migrants and 108 non-migrants without abnormal findings in gastroscopy examination at a hospital in Changzhi city from May 2014 to June 2015. Plasma FA was detected with enzyme-linked immunosorbent assay(ELISA) and MTHFR and MS gene polymorphisms were analyzed with polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)among the participants. Results There was a significant difference in plasma FA level between the migrants and nonmigrants(P = 0.006). Among the migrants, the plasma FA level differed significantly by gender, age, and whether drinking alcohol(P < 0.05 for all); while, among the non-migrants, FA level differed significantly by blood type(P < 0.05). There were significant differences in plasma FA levels between the migrants and non-migrants for the participants aged > 60 years,with the body mass index(BMI) of < 18.5 kg/m2, non-smokers, and non-alcohol drinkers(P < 0.05 for all). The MTHFR and MS genotypes and allele frequencies were different significantly between the migrants and non-migrants(all P < 0.05).Conclusion The plasma FA level is different between migrants and non-migrants and various subgroups of the two populations. The distribution of MTHFR and MS gene polymorphisms are also significantly different between the two population groups.
Objective To investigate the content of plasma folic acid(FA) and the gene polymorphisms of methylenetetrahydrofolate reductase(MTHFR C677 T) and methionine synthase(MS A2756 G) among migrants and nonmigrants in Changzhi city of Shanxi province, and to provide evidences for further research. Methods We recruited 80 migrants and 108 non-migrants without abnormal findings in gastroscopy examination at a hospital in Changzhi city from May 2014 to June 2015. Plasma FA was detected with enzyme-linked immunosorbent assay(ELISA) and MTHFR and MS gene polymorphisms were analyzed with polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)among the participants. Results There was a significant difference in plasma FA level between the migrants and nonmigrants(P = 0.006). Among the migrants, the plasma FA level differed significantly by gender, age, and whether drinking alcohol(P < 0.05 for all); while, among the non-migrants, FA level differed significantly by blood type(P < 0.05). There were significant differences in plasma FA levels between the migrants and non-migrants for the participants aged > 60 years,with the body mass index(BMI) of < 18.5 kg/m2, non-smokers, and non-alcohol drinkers(P < 0.05 for all). The MTHFR and MS genotypes and allele frequencies were different significantly between the migrants and non-migrants(all P < 0.05).Conclusion The plasma FA level is different between migrants and non-migrants and various subgroups of the two populations. The distribution of MTHFR and MS gene polymorphisms are also significantly different between the two population groups.
引文
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[11]陈云霞,武延隽,申丽丽.育龄女性叶酸代谢相关酶基因多态性分析[J].中国公共卫生,2010,26(8):958-959.
[12]王秀梅,张卫群,陈波,等.MTHFR基因多态性与哈萨克族食管癌易感性[J].中国公共卫生,2007,23(8):937-938.
[2]黄之敏,林征,李晓燕,等.MTHFR C667T基因多态性与食管癌易感性关系Meta分析[J].中国公共卫生,2013,29(11):1690-1694.
[3]Yu K,Zhang J,Zhang J,et al.Methionine synthase A2756G polymorphism and cancer risk:a meta-analysis[J].Eur J Hum Genet,2010,18(3):370-378.
[4]杨建洲,纪爱芳,王金胜,等.太行山地区食管癌高发区人群血浆叶酸水平与食管鳞癌关系的研究[J].中华内科杂志,2016,55(4):317-319.
[5]Wen YY,Yang SJ,Zhang JX,et al.Association of methylenetetrahydrofolate reductase genetic polymorphisms and folate intake with susceptibility of esophageal squamous cell carcinoma susceptibility:a meta-analysis of case-control studies[J].Asian Pac J Cancer Prev,2013,14(1):21-25.
[6]Cai C,Xiao R,Halm-Lutterodt NV,et al.Association of MTHFR,SLC19A1 genetic polymorphism,serum folate,vitamin B12 and hcy status with cognitive functions in Chinese adults[J].Nutrients,2016,8(10):665-679.
[7]Yang BY,Liu YY,Li YF,et al.Geographical distribution of MTHFR C677T,A1298C and MTRR A66G gene polymorphisms in China:findings from 15357 adults of Han nationality[J].PLoS One,2013,8(3):e57917.
[8]Saraswathy KN,Asghar M,Samtani R,et al.Spectrum of MTHFR gene SNPs C677T and A1298C:a study among 23 population groups of India[J].Mol Biol Rep,2012,39(4):5025-5031.
[9]Mao R,Fan Y,Chen F,et al.Methylenetetrahydrofolate reductase gene polymorphisms in 13 Chinese ethnic populations[J].Cell Biochem Funct,2008,26(3):352-358.
[10]谢渊,张婷,李毅,等.贵州布依族、侗族和苗族人群同型半胱氨酸代谢酶相关MS及MSR基因多态性分析[J].中华医学遗传学杂志,2012,29(6):723-727.
[11]陈云霞,武延隽,申丽丽.育龄女性叶酸代谢相关酶基因多态性分析[J].中国公共卫生,2010,26(8):958-959.
[12]王秀梅,张卫群,陈波,等.MTHFR基因多态性与哈萨克族食管癌易感性[J].中国公共卫生,2007,23(8):937-938.