基于PLGA的纳米基因载体及其在癌症治疗中的应用进展
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摘要
随着基因用于癌症治疗研究进展,寻找安全、有效载体将目的基因高效准确运送至癌症部位,成为制剂学研究的重点。本文通过归纳总结近年国内外的文献报道,对PLGA的纳米基因载体用于癌症治疗进行综述性研究。探讨PLGA纳米粒作为癌症治疗基因载体的潜在临床应用价值,期望对PLGA纳米基因载药系统的应用有更深层次的了解。结果显示PLGA是一种极具潜力的纳米粒基因载体材料,用于制备基因载体,具有极大的应用价值,且被广泛应用于癌症治疗实验研究中,对癌症的治疗有极大的潜在临床应用价值
As the gene is used for cancer treatment research,it is a key point to find safe and effective vectors to accurately transport the target genes to cancer sites. We summarized recent reports about the potential clinical application value of PLGA [poly( lactic-co-glycolicacid) ] nanoparticles as cancer gene carriers and provided a deeper understanding of the application of PLGA nano-gene carrier system. PLGA as a kind of biodegradable material is of great application value in terms of delivering anti-cancer genes,the PLGA nanoparticle carrier is widely used in experimental study of cancer gene therapy and also expected to bring hope for human cancer treatment.
As the gene is used for cancer treatment research,it is a key point to find safe and effective vectors to accurately transport the target genes to cancer sites. We summarized recent reports about the potential clinical application value of PLGA [poly( lactic-co-glycolicacid) ] nanoparticles as cancer gene carriers and provided a deeper understanding of the application of PLGA nano-gene carrier system. PLGA as a kind of biodegradable material is of great application value in terms of delivering anti-cancer genes,the PLGA nanoparticle carrier is widely used in experimental study of cancer gene therapy and also expected to bring hope for human cancer treatment.
引文
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[27]Tang J,He J F,Yang C L,et al.Antitumor effects of survivin T34A-Ca Pi complex embedded PLGA nanoparticles in combination with doxcil in mice[J].J Nanopart Res,2014,16(11):2682(1-17).
[28]Hou X Y,Yang C L,Zhang L J,et al.Killing colon cancer cells through PCD pathways by a novel hyaluronic acidmodified shell-core nanoparticle loaded with RIP3 in combination with chloroquine[J].Biomaterials,2017,124:195-210.
[2]Wang X,Li Y,Liu S,et al.Direct activation of RIP3/MLKL-dependent necrosis by herpes simplex virus 1(HSV-1)protein ICP6 triggers host antiviral defense[J].Proc Natl Acad Sci USA,2014,111(43):15438–15443.
[3]Yang C L,Hu T T,Cao H,et al.Chloroquine containing PLGA based p DNA delivery system for tumor and inflammatory targeting in vitro and in vivo[J].Mol Pharm,2015,12(6):2167-2179.
[4]Mokuda S,Miyazaki T,Ito Y,et al.The proto-oncogene survivin splice variant 2B is induced by PDGF and leads to cell proliferation in rheumatoid arthritis fibroblast-like synoviocytes[J].Sci Rep,2015,5:9795(1-13).
[5]Hamil A S,Dowdy S F.Synthesis and Conjugation of Small Interfering Ribonucleic Neutral SiRNNs[J].Methods Mol Biol,2016,1364:1-9.
[6]Chen Y,Li Y Q,Gao J B,et al.Enzymatic PEGylated Poly(lactone-co-β-aminoester)Nanoparticles as Biodegradable,Biocompatible and Stable Vectors for Gene Delivery[J].ACS Appl Mater Interfaces,2016,8(1):490-501.
[7]Pavlov A M,Sukhorukov G B,Gould D J.Location of molecules in layer-by-layer assembled microcapsules influences activity,cell delivery and susceptibility to enzyme degradation[J].J Control Release,2013,172(1):22-29.
[8]付敏,徐希明,余江南.PLGA多孔支架的制备及在基因传递系统中的应用研究进展[J].药学进展,2013,3(8):376-382.
[9]陈红丽,秦靖雯,南文滨,等.多功能化乳酸-羟基乙酸共聚物纳米粒作为抗肿瘤药物载体研究进展[J].新乡医学院学报,2016,33(5):349-351.
[10]Park J,Fong P M,Lu J,et al.Paclitaxel-loaded PEGylated PLGA-based nanoparticles in vitro and in vivo evaluation[J].J Control Release,2009,13(1):11-17.
[11]李洪英,邹伟伟,刘春喜.阳离子PLGA载基因纳米粒的初步研究[J].中国生化药物杂志,2009,30(2):78-81.
[12]Jeong Y I,Kimdo H,Chung C W,et al.Self-assembled nanoparticles of hyaluronic acid/poly(DL-lactide-coglycolide)block copolymer[J].Colloids Surf B Biointerfaces,2012,90(4):28-35.
[13]Desai M P,Labhasetwar V,Amidon G L,et al.Gastrointestinal uptake of biodegradable microparticles:effect of particle size[J].Pharm Res,1997,13(12):1838-1845.
[14]张清.生物可降解聚合物作为非病毒载体的研究进展[J].食品与药品杂志,2009,11(11):61-64.
[15]毛立丽,刘洁,杨亚妮,等.聚乳酸-羟基乙酸纳米粒表面功能化修饰及其应用[J].中国医药工业杂志,2012,43(11):954-958.
[16]Takashima Y,Saito R,Nakajiam A,et al.Spray-drying preparation of microparticles containing cationic PLGA nanospheres as gene carriers for avoiding aggregation of nanospheres[J].Int J Pharm,2007,343(1-2):262-269
[17]涂心明,郭绍芳,胡伊乐.MPEG-PLGA纳米胶囊在真核质粒转染细胞中的应用[J].药物杂志,2012,3(3):178-183.
[18]邹伟伟,张娜.聚乳酸-聚乙醇酸纳米粒作为非病毒基因载体[J].生命的化学,2009,21(1):120-124.
[19]唐薇薇,郑洪.RNA干扰survivin对卵巢癌HO-8910PM细胞周期的影响[J].遵义医学院学报,2013,36(5):413-416.
[20]邓力蔚.基因与药物共运输的PLGA超声纳米泡的抗肿瘤作用及超声成像研究[D].成都:电子科技大学,2014.
[21]Zhou J,Patel T R,Fu M,et al.Octa-functional PLGA nanoparticles for targeted efficient siRNAN delivery to tumors[J].Biomaterials,2012,33(2):583-591.
[22]Kim T I,Lee M,Kim S W.A guanidinylated bioreducible polymer with high nuclear localization ability for gene delivery systems[J].Biomaterials,2010,31(7):1798–1804.
[23]崔盼盼,陈超,赵娟娟,等.非病毒载体递送微小RNA治疗肿瘤的研究进展[J].遵义医学院学报,2016,39(1):92-99.
[24]Cosco D,Cilurzo F,Paolino D,et al.Delivery of miR-34a by chitosan/PLGA nanoplexes for the anticancer treatment of multiple myeloma[J].Sci Rep,2015,5:17579(1-11).
[25]Zhang Y D,Pan A H.Nanotechnology-DNA opertions[J].Chin J Chem Eng,2003,11(6):22-29.
[26]Little S R,Lynn D M,Puram S V,et al.Formulation and characterization of poly(beta amino esster)microparticles for genetic vaccine delivery[J].J Control Release,2005,107(3):449-462.
[27]Tang J,He J F,Yang C L,et al.Antitumor effects of survivin T34A-Ca Pi complex embedded PLGA nanoparticles in combination with doxcil in mice[J].J Nanopart Res,2014,16(11):2682(1-17).
[28]Hou X Y,Yang C L,Zhang L J,et al.Killing colon cancer cells through PCD pathways by a novel hyaluronic acidmodified shell-core nanoparticle loaded with RIP3 in combination with chloroquine[J].Biomaterials,2017,124:195-210.