基于纳米基因载体的动植物遗传转化研究进展
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摘要
转基因技术在动植物优良新品种的培育中发挥着重要作用,而随着纳米生物技术的发展,基于纳米材料构建基因载体的动植物转基因技术,对于发展动植物转基因新方法以及加速转基因种质材料的大规模制备、优良新品种的培育进程具有更为重要的意义。综述了纳米基因载体的种类与性质,并结合动植物遗传育种的研究进展,分析了纳米基因载体相比于其他载体的特点及优势,同时,重点阐述了基于纳米基因载体的基因转染技术的基本原理和操作过程,及其在动植物遗传转化中的应用,以期为动植物基因工程改造提供新思路。
Transgenic technology plays an important role in the cultivation of excellent new varieties of animals and plants. With the development of nanobiotechnology,genetic transformation technology of animals and plants,which is based on gene vector constructed by nano-materials,is of great significance in developing novel transgenic methods for animals and plants,accelerating the largescale preparation of genetically modified germplasm materials and the process of cultivating new varieties. The types and properties of nano-gene vectors were reviewed. And in combination with the progress on genetics and breeding of animals and plants,the characteristics and advantages of nano-gene vectors compared with other vectors were analyzed. In the meantime,the basic principle and operation process of gene transfection technology based on nano-gene carrier,as well as its application in genetic transformation of animals and plants were expounded emphatically,which was expected to provide a new thought for genetic engineering transformation of animals and plants.
Transgenic technology plays an important role in the cultivation of excellent new varieties of animals and plants. With the development of nanobiotechnology,genetic transformation technology of animals and plants,which is based on gene vector constructed by nano-materials,is of great significance in developing novel transgenic methods for animals and plants,accelerating the largescale preparation of genetically modified germplasm materials and the process of cultivating new varieties. The types and properties of nano-gene vectors were reviewed. And in combination with the progress on genetics and breeding of animals and plants,the characteristics and advantages of nano-gene vectors compared with other vectors were analyzed. In the meantime,the basic principle and operation process of gene transfection technology based on nano-gene carrier,as well as its application in genetic transformation of animals and plants were expounded emphatically,which was expected to provide a new thought for genetic engineering transformation of animals and plants.
引文
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[2]Li Y,Gao J,Zhang C,et al..Stimuli-responsive polymeric nanocarriers for efficient gene delivery[J].Top.Curr.Chem.,2017,375(2):27.
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[9]肖苏尧,刘选明,童春义,等.多聚赖氨酸淀粉纳米颗粒基因载体的研制及应用[J].中国科学:B辑,2004,34(6):473-477.
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[14]Zhang Y,Shu H,Hu J,et al..Binding affinity,cellular uptake,and subsequent intracellular trafficking of the nano-gene vector P123-PEI-R13[J].J.Nanomater.,2016:7064246.
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[17]Chen R,Song G,Wei Y.Synthesis of variable-sized Fe3O4nanocrystals by visible light irradiation at room temperature[J].J.Phys.Chem.C,2010,114(32):13409-13413.
[18]Liu Y,Jia S,Wu Q,et al..Studies of Fe3O4-chitosan nanoparticles prepared by co-precipitation under the magnetic field for lipase immobilization[J].Catal.Commun.,2011,12(8):717-720.
[19]Yu M,Gu Z,Ottewell T,et al..Silica-based nanoparticles for therapeutic protein delivery[J].J.Mater.Chem.B,2017,5(18):3241-3252.
[20]Fu Y Q,Li L H Wang H,et al..Silica nanoparticles-mediated stable genetic transformation in Nicotiana tabacum[J].Chem.Res.Chinese U.,2015,31(6):976-981.
[21]杜伟,崔海信,王琰,等.精子载体法制备转基因动物的研究进展[J].生物技术通报,2012(12):13-18.
[22]余东升,黄洪章,李苏,等.转基因载体聚乙二醇-聚谷氨酸纳米微球的制备[J].实用癌症杂志,2004,19(1):5-8.
[23]Wang P,Lombi E,Zhao F J,et al..Nanotechnology:A new opportunity in plant sciences[J].Trends Plant Sci.,2016,21(8):699-712.
[24]Bally M B,Harvie P,Wong F M P,et al..Biological barriers to cellular delivery of lipid-based DNA carriers[J].Adv.Drug Deliver.Rev.,1999,38(3):291-315.
[25]Shahzad A,Parveen S,Sharma S,et al..Plant tissue culture:Applications in plant improvement and conservation[A].In:Abdin M Z,Kiran U,Ali A.Plant biotechnology:Principles and applications[M].Berlin:Springer,2017,37-72.
[26]Singh R K,Prasad M.Advances in Agrobacterium tumefaciensmediated genetic transformation of graminaceous crops[J].Protoplasma,2016,253(3):691-707.
[27]李菲,张淑江,章时蕃,等.基因枪法介导大白菜小孢子转基因技术研究初报[J].园艺学报,2017,44(1):62-68.
[28]Soto N,Delgado C,Hernández Y,et al..Efficient particle bombardment-mediated transformation of Cuban soybean(INCASoy-36)using glyphosate as a selective agent[J].Plant Cell Tiss.Org.,2017,128(1):187-196.
[29]Yang L,Cui G,Wang Y,et al..Expression of foreign genes demonstrates the effectiveness of pollen-mediated transformation in Zea mays[J].Front.Plant Sci.,2017,8:383.
[30]南芝润,惠国强,罗绮,等.超声波辅助花粉介导法获得转Bt Cry1Ac基因玉米的研究[J].黑龙江农业科学,2015(9):22-25.
[31]赵小光,张耀文,关周博,等.花粉在植物遗传研究中的应用[J].中国农学通报,2017(13):54-59.
[32]Ali A,Bang S W,Chung S M,et al..Plant transformation via pollen tube-mediated gene transfer[J].Plant Mol.Biol.Rep.,2015,33(3):742-747.
[33]Touraev A,St9ger E,Voronin V,et al..Plant male germ line transformation[J].Plant J.,1997,12(4):949-956.
[34]朱宁.棉花农杆菌花粉管通道遗传转化方法的建立与转基因高油棉花种质的创造及其应用[D].杭州:浙江大学,硕士学位论文,2014.
[35]赵翔.基于四氧化三铁纳米磁转化系统的花粉介导棉花转基因技术[D].北京:中国农业科学院,博士学位论文,2015.
[36]杨慧珍,任志强,肖建红,等.花粉介导法获得耐草甘膦玉米植株及其耐性研究[J].生物技术通报,2016,32(11):152-161.
[37]翟亦倩,罗昌,程曦,等.一种利用花粉管结合农杆菌介导方法将iaa M基因导入玉米自交系的研究[J].安徽农业科学,2013,41(8):3336-3338.
[38]郭天璐,张欢欢,杜建中,等.花粉介导的花青素调节基因Nt An2转化玉米研究[J].山西农业科学,2016,44(4):427-431.
[39]王长彪,杜建中,赵兴华,等.超声波辅助花粉介导转基因方法的研究进展[J].山西农业科学,2017,45(9):1571-1576.
[40]Kim S S,Shin D I,Park H S.Transientβ-glucuronidase expression in lily(Lilium longflorum L.)pollen via wounding-assisted Agrobacterium-mediated transformation[J].Biotechnol.Lett.,2007,29(6):965-969.
[41]古晓晓,杜宝吉,李云辉,等.基于纳米材料的基因载体[J].化学进展,2015,27(8):1093-1101.
[42]Torney F,Trewyn B G,Lin V S Y,et al..Mesoporous silica nanoparticles deliver DNA and chemicals into plants[J].Nat.Nanotechnol.,2007,2(5):295-300.
[43]Zhao X,Meng Z,Wang Y,et al..Pollen magnetofection for genetic modification with magnetic nanoparticles as gene carriers[J].Nat.Plants,2017,3(12):956.
[44]王凤华,刘俊,童春义,等.电击法磁性纳米颗粒作为水稻转基因载体的研究[J].分析化学,2010,38(5):617-621.
[45]刘俊.基于纳米颗粒的植物转基因及其检测研究[D].长沙:湖南大学,博士学位论文,2005.
[46]宋瑜.植物纳米基因载体及其细胞转化效果研究[D].北京:中国农业科学院,硕士学位论文,2009.
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