没食子酸修饰纳米硒的制备表征及其抑菌活性研究
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摘要
在酸性条件下加热没食子酸(Gallic acid,GA)还原亚硒酸钠制得没食子酸修饰的纳米硒(Gallic acid modified selenium nanoparticle,GA@Se),并通过紫外可见光光谱和透射电镜对其进行结构表征,同时用滤纸片抑菌法、带毒平板法和比浊法测定GA@Se及GA对金黄色葡萄球菌、大肠杆菌的抑菌效果。结果显示:所制备的GA@Se呈现球形形貌,且粒径较小,分散均匀。纳米粒子表面所负载的GA浓度达到3.3 mg/mL。在低浓度下,GA对金黄色葡萄球菌和大肠杆菌的抑菌效果较差,然而,GA@Se对金黄色葡萄球菌与大肠杆菌都有较好的抑菌效果,其对金黄色葡萄球菌与大肠杆菌的半数抑菌浓度分别是18.01、394.28μg/mL。因而,GA@Se有望成为一种高活性的纳米类抗菌剂。
Gallic acid modified selenium nanoparticle(GA@Se) was synthesised by heating Gallic acid(GA) to reduce the sodium selenite under acidic conditions, and it was characterized by UVVis spectrophotometer and Transmission Electron Microscope. The antibacterial effect of GA@Se and GA on Staphylococcus aureus and Escherichia coli was determined by paper filtering method, radial growth assay and turbidimetry method. The results investigated that synthesised GA@Se had a spherical morphology with small particle size and uniform dispersion. The concentration of GA reached 3.3 mg/mL, which was loaded on the surface of nanoparticles. At low concentrations, the ability of GA inhibited Staphylococcus aureus and Escherichia coli was inferior to GA@Se. However, GA@Se had a noticeable antibacterial activity against Staphylococcus aureus and Escherichia coli. The half-inhibitory concentrations of Staphylococcus aureus and Escherichia coli were 18.01 μg/mL and 394.28 μg/mL, respectively. Thus, GA@Se is expected to develope a nano-antibacterial agent efficiently.
Gallic acid modified selenium nanoparticle(GA@Se) was synthesised by heating Gallic acid(GA) to reduce the sodium selenite under acidic conditions, and it was characterized by UVVis spectrophotometer and Transmission Electron Microscope. The antibacterial effect of GA@Se and GA on Staphylococcus aureus and Escherichia coli was determined by paper filtering method, radial growth assay and turbidimetry method. The results investigated that synthesised GA@Se had a spherical morphology with small particle size and uniform dispersion. The concentration of GA reached 3.3 mg/mL, which was loaded on the surface of nanoparticles. At low concentrations, the ability of GA inhibited Staphylococcus aureus and Escherichia coli was inferior to GA@Se. However, GA@Se had a noticeable antibacterial activity against Staphylococcus aureus and Escherichia coli. The half-inhibitory concentrations of Staphylococcus aureus and Escherichia coli were 18.01 μg/mL and 394.28 μg/mL, respectively. Thus, GA@Se is expected to develope a nano-antibacterial agent efficiently.
引文
[1]罗爱国,冯佳,胡变芳,等.7种可食藻提取物对食品致病菌的抑菌效果[J].江苏农业科学,2018,46(5):162-167
[2]史贤明,施春雷,索标,等.食品加工过程中致病菌控制的关键科学问题[J].中国食品学报,2011,11(9):194-208
[3]张彬,刘笑宇,姜萍萍,等.肉桂醛对大肠杆菌和金黄色葡萄球菌的抑菌作用及抑菌机制研究[J].现代食品科技,2015,31(5):31-35
[4]徐振波,刘晓晨,李琳,等.金黄色葡萄球菌肠毒素在食源性微生物中的研究进展[J].现代食品科技,2013,29(9):2317-2324
[5]向红,周藜,瘳春,等.金黄色葡萄球菌及其引起的食物中毒的研究进展[J].中国食品卫生杂志,2015,27(2):196-199
[6]石慧,陈卓逐,阚建全.大肠杆菌在食品加工贮藏中胁迫响应机制的研究进展[J].食品科学,2016,37(9):250-257
[7]郑雪花,杨君,杨跃辉.没食子酸药理作用的研究进展[J].中国医院药学杂志,2017,37(1):94-98
[8]胡梦,程玉冰,张培燕,等.百药煎中转化鞣质生成没食子酸的最佳菌种组合筛选[J].中国现代中药,2018,20(4):453-457
[9]卢静,任梦柔,陈锐,等.没食子酸对荧光假单胞菌的抑制作用研究[J].食品科技,2015,40(6):300-303
[10]张雅丽.五倍子没食子酸的制备、抑菌作用及机制研究[D].西安:陕西师范大学,2013:1-8
[11]王珂佳,任艳玲.硒元素对博落回有效药用成分抑菌效果的影响[J].黑龙江畜牧兽医,2016,(3):194-196
[12] Zhang J S, Wang X F, Xu T W. Elemental Selenium at Nano Size(Nano-Se)as a Potential Chemopreventive Agent with Reduced Risk of Selenium Toxicity:Comparison with Se-Methylselenocysteine in Mice[J].Toxicological Sciences,2008,101(1):22-31
[13]路玲,李莉.罗自生.纳米抗菌剂在食品中的应用研究进展[J].食品与发酵工业,2018:1-7
[14]代小英,许欣,陈昭斌,等.纳米抗菌剂的概况[J].现代预防医学,2008,35(13):2513-2515
[15] Huang X Q, Chen X, Chen Q C, et al. Investigationof functional selenium nanoparticles as potent antimicrobial agents against superbugs[J]. Acta biomaterialia,2016,30:397-407
[16]钟彩玲.纳米硒界面对草酸钙晶体生长的调控作用[D].广州:暨南大学,2014:28-38
[17]杨小慧,石光波,拜晓彬,等.文冠果落果黄铜成分分析及抑菌性评价[J].食品科学,2018,39(10):53-58
[18]张智,刘慧,刘奇,等.玉米肽-锌螯合物结构表征及抗氧化活性分析[J].食品科学,2017,38(3):131-135
[19]杨志恒,武伟,刘泽华,等.纳米TiO2/β-CDP复合物的制备及表征[J].天津科技大学学报,2018,33(2):38-42
[2]史贤明,施春雷,索标,等.食品加工过程中致病菌控制的关键科学问题[J].中国食品学报,2011,11(9):194-208
[3]张彬,刘笑宇,姜萍萍,等.肉桂醛对大肠杆菌和金黄色葡萄球菌的抑菌作用及抑菌机制研究[J].现代食品科技,2015,31(5):31-35
[4]徐振波,刘晓晨,李琳,等.金黄色葡萄球菌肠毒素在食源性微生物中的研究进展[J].现代食品科技,2013,29(9):2317-2324
[5]向红,周藜,瘳春,等.金黄色葡萄球菌及其引起的食物中毒的研究进展[J].中国食品卫生杂志,2015,27(2):196-199
[6]石慧,陈卓逐,阚建全.大肠杆菌在食品加工贮藏中胁迫响应机制的研究进展[J].食品科学,2016,37(9):250-257
[7]郑雪花,杨君,杨跃辉.没食子酸药理作用的研究进展[J].中国医院药学杂志,2017,37(1):94-98
[8]胡梦,程玉冰,张培燕,等.百药煎中转化鞣质生成没食子酸的最佳菌种组合筛选[J].中国现代中药,2018,20(4):453-457
[9]卢静,任梦柔,陈锐,等.没食子酸对荧光假单胞菌的抑制作用研究[J].食品科技,2015,40(6):300-303
[10]张雅丽.五倍子没食子酸的制备、抑菌作用及机制研究[D].西安:陕西师范大学,2013:1-8
[11]王珂佳,任艳玲.硒元素对博落回有效药用成分抑菌效果的影响[J].黑龙江畜牧兽医,2016,(3):194-196
[12] Zhang J S, Wang X F, Xu T W. Elemental Selenium at Nano Size(Nano-Se)as a Potential Chemopreventive Agent with Reduced Risk of Selenium Toxicity:Comparison with Se-Methylselenocysteine in Mice[J].Toxicological Sciences,2008,101(1):22-31
[13]路玲,李莉.罗自生.纳米抗菌剂在食品中的应用研究进展[J].食品与发酵工业,2018:1-7
[14]代小英,许欣,陈昭斌,等.纳米抗菌剂的概况[J].现代预防医学,2008,35(13):2513-2515
[15] Huang X Q, Chen X, Chen Q C, et al. Investigationof functional selenium nanoparticles as potent antimicrobial agents against superbugs[J]. Acta biomaterialia,2016,30:397-407
[16]钟彩玲.纳米硒界面对草酸钙晶体生长的调控作用[D].广州:暨南大学,2014:28-38
[17]杨小慧,石光波,拜晓彬,等.文冠果落果黄铜成分分析及抑菌性评价[J].食品科学,2018,39(10):53-58
[18]张智,刘慧,刘奇,等.玉米肽-锌螯合物结构表征及抗氧化活性分析[J].食品科学,2017,38(3):131-135
[19]杨志恒,武伟,刘泽华,等.纳米TiO2/β-CDP复合物的制备及表征[J].天津科技大学学报,2018,33(2):38-42