褐藻胶寡糖食用安全性毒理学评价
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摘要
目的:对褐藻胶寡糖的食用安全性进行毒理学评价。方法:采用小鼠急性经口毒性试验、遗传毒性试验(Ames试验、小鼠骨髓细胞微核试验、小鼠精母细胞染色体畸变试验)和大鼠28天经口毒性试验。结果:小鼠急性经口半数致死剂量(LD50)大于15000 mg/kg BW;Ames试验、小鼠骨髓细胞微核试验、小鼠精母细胞染色体畸变试验结果均为阴性;在28天经口毒性试验中,大鼠的生长发育、血液学、血生化、尿常规及病理组织学检查未见与受试物相关的异常变化。结论:在试验剂量范围内,褐藻胶寡糖为实际无毒,无遗传毒性,28天经口毒性试验未发现明显毒性反应。
Objective:To assess the edible safety of alginate oligosaccharide. Methods:The toxicity of alginate oligosaccharide was investigated by acute oral test, genetic toxicity test including Ames test, mice marrow cell micronucleus test and mice spermatocyte chromosome aberration test, and 28-day oral toxicity test in rats. Results:The acute oral LD50(50% lethal dose) in mice was over 15000 mg/kg BW. No evidences of genotoxic activity to mice were foud. BY 28-day oral toxicity test, no significant changes in organ weight, hematologic,serum biochemical and urinalysis were noted, and no test substance-related pathological changes were found. Conclusion:No acute oral toxicity or genotoxicity of alginate oligosaccharide was found in this test dose range, and the toxicity of alginate oligosaccharide was not observed at the end of 28-day oral toxicity test.
Objective:To assess the edible safety of alginate oligosaccharide. Methods:The toxicity of alginate oligosaccharide was investigated by acute oral test, genetic toxicity test including Ames test, mice marrow cell micronucleus test and mice spermatocyte chromosome aberration test, and 28-day oral toxicity test in rats. Results:The acute oral LD50(50% lethal dose) in mice was over 15000 mg/kg BW. No evidences of genotoxic activity to mice were foud. BY 28-day oral toxicity test, no significant changes in organ weight, hematologic,serum biochemical and urinalysis were noted, and no test substance-related pathological changes were found. Conclusion:No acute oral toxicity or genotoxicity of alginate oligosaccharide was found in this test dose range, and the toxicity of alginate oligosaccharide was not observed at the end of 28-day oral toxicity test.
引文
[1] Haug A.Alginicacid:isolation and fractionation with potassium chloride and manganous ions[J].Meth Carbohydr Chem,1965,5:69-73.
[2] Zhang Z Q,Yu G L,Guan H S,et al.Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibrosp.510[J].Carbohydr Res,2004,339:1475-1481.
[3] Philippe P,Kamal B,Frithjof K,et al.Oligosaccharide recognition signals and defence reactions in marine plant-microbe interactions[J].Ecology and Microbiology,1999,2(3):276-283.
[4] 管华诗,蓝进,崔英林,等.褐藻胶酸钠制剂治疗糖尿病的研究[J].中国海洋药物,1983,2(3):153.
[5] 刘斌,王长云.海藻多糖褐藻胶生物活性及其应用研究新进展[J].中国海洋药物,2004,23(6):36-41.
[2] Zhang Z Q,Yu G L,Guan H S,et al.Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibrosp.510[J].Carbohydr Res,2004,339:1475-1481.
[3] Philippe P,Kamal B,Frithjof K,et al.Oligosaccharide recognition signals and defence reactions in marine plant-microbe interactions[J].Ecology and Microbiology,1999,2(3):276-283.
[4] 管华诗,蓝进,崔英林,等.褐藻胶酸钠制剂治疗糖尿病的研究[J].中国海洋药物,1983,2(3):153.
[5] 刘斌,王长云.海藻多糖褐藻胶生物活性及其应用研究新进展[J].中国海洋药物,2004,23(6):36-41.