双酸酸蚀钛表面复合含钙纳米薄片膜层对成骨细胞行为的影响
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摘要
目的:制备含钙纳米薄片膜层修饰的双酸酸蚀钛表面并评价其对成骨细胞行为的影响。方法:通过双酸酸蚀和氢氧化钙/双氧水混合溶液水热处理,制备2种含钙纳米薄片膜层修饰的双酸酸蚀钛表面(1 h、6 h处理组)。以大颗粒喷砂酸蚀(SLA)钛表面为对照组,2种含钙纳米薄片膜层修饰的双酸酸蚀钛表面为实验组,观察分析不同钛表面的微形貌和表面元素组成;将MC3T3-E1成骨细胞接种于各组试件表面,研究不同钛表面对成骨细胞生物学行为的影响。结果 :在双酸酸蚀钛表面制备形成2种形貌均一的纳米薄片膜层结构,均含有微量钙元素。相比于SLA钛表面,2种新型钛表面均有利于MC3T3-E1成骨细胞的黏附和增殖,显著增强成骨细胞的碱性磷酸酶活性,并上调成骨相关基因的表达。其中,1 h处理组性能更优。结论:双酸酸蚀钛表面复合含钙纳米薄片膜层能有效促进成骨细胞的黏附、增殖和分化。
Objective: To prepare the double acid etching titanium surface modified with calcium-containing nanoflakes and evaluate its effe cts on osteoblast behaviors in vitro. Methods: Through double acid-etching and hydrothermal treatment with a mixed solution of Ca(OH)2/H2 O2, two different calcium-containing nanoflakes(the 1 h and the 6 h treatment groups) were synthesized on the double acid etching titanium surfaces. The sandblasting with large grit and acid etching(SLA) titanium surface was used as the control group, and two double acid etching titanium surfaces modified with different calcium-containing nanoflakes were used as two experiment groups. The micro-topography and surface elemental compositions o f different titanium surfaces were observed and analyzed.MC3 T3-E1 cells were seeded on samples of the three groups to investiga te osteoblast behaviors on different titanium surfaces.Results : Two uniform nanoflake-like structures containing trace calcium were prepa red on double acid etching titanium surfaces.Compared with the SLA titanium surface, two modified titanium surfaces significantly up-regulated the adhesion, proliferation, ALP activity and expressions of osteogenic-related genes of MC3 T3-E1 cells, especially the 1 h treatment group. Conclusion: The double acid etching titanium surface modified with calcium-containing nanoflakes could promote the adhesion, proliferation and differentiation of osteoblasts.
Objective: To prepare the double acid etching titanium surface modified with calcium-containing nanoflakes and evaluate its effe cts on osteoblast behaviors in vitro. Methods: Through double acid-etching and hydrothermal treatment with a mixed solution of Ca(OH)2/H2 O2, two different calcium-containing nanoflakes(the 1 h and the 6 h treatment groups) were synthesized on the double acid etching titanium surfaces. The sandblasting with large grit and acid etching(SLA) titanium surface was used as the control group, and two double acid etching titanium surfaces modified with different calcium-containing nanoflakes were used as two experiment groups. The micro-topography and surface elemental compositions o f different titanium surfaces were observed and analyzed.MC3 T3-E1 cells were seeded on samples of the three groups to investiga te osteoblast behaviors on different titanium surfaces.Results : Two uniform nanoflake-like structures containing trace calcium were prepa red on double acid etching titanium surfaces.Compared with the SLA titanium surface, two modified titanium surfaces significantly up-regulated the adhesion, proliferation, ALP activity and expressions of osteogenic-related genes of MC3 T3-E1 cells, especially the 1 h treatment group. Conclusion: The double acid etching titanium surface modified with calcium-containing nanoflakes could promote the adhesion, proliferation and differentiation of osteoblasts.
引文
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[2]Huang Y,He J,Gan L,et al.Osteoconductivity and osteoinductivity of porous hydroxyapatite coatings deposited by liquid precursor plasma spraying:in vivo biological response study[J].Biomed Mater,2014,9(6):065007
[3]Ballo A,Agheli H,Lausmaa J,et al.Nanostructured model implants for in vivo studies:influence of well-defined nanotopography on de novo bone formation on titanium implants[J].Int J Nanomedicine,2011,6(1):3415-3428
[4]Wang XX,Hayakawa S,Tsuru K,et al.Bioactive titania-gel layers formed by chemical treatment of Ti substrate with a H2O2/HCl solution[J].Biomaterials,2002,23(5):1353-1357
[5]Ding X,Zhou L,Wang J,et al.The effects of hierarchical micro/nanosurfaces decorated with Ti O2nanotubes on the bioactivity of titanium implants in vitro and in vivo[J].Int J Nanomedicine,2015,10:6955-6973
[6]Rho JY,Kuhn-Spearing L,Zioupos P.Mechanical properties and the hierarchical structure of bone[J].Med Eng Phys,1998,20(2):92-102
[7]Cheng M,Qiao Y,Wang Q,et al.Calcium plasma implanted titanium surface with hierarchical microstructure for improving the bone formation[J].ACS Appl Mater Interfaces,2015,7(23):13053-13061
[8]Liu C,Zhang Y,Wang L,et al.A strontium-modified titanium surface produced by a new method and its biocompatibility in vitro[J].PLo S One,2015,10(11):e0140669
[9]Cavalcanti-Adam EA,Volberg T,Micoulet A,et al.Cell spreading and focal adhesion dynamics are regulated by spacing of integrin ligands[J].Biophys J,2007,92(8):2964-2974
[10]王程越,马红梅,赵宝红,等.表面经喷砂和酸蚀及羟基磷灰石涂层的TA2纯钛种植体对富血小板血浆体外骨诱导能力的影响[J].口腔医学,2007,27(7):343-346
[11]Bressan E,Sbricoli L,Guazzo R,et al.Nanostructured surfaces of dental implants[J].Int J Mol Sci,2013,14(1):1918-1931
[12]Liu H,Webster TJ.Mechanical properties of dispersed ceramic nanoparticles in polymer composites for orthopedic applications[J].Int J Nanomedicine,2010,5(5):299-313
[13]Webster TJ,Ergun C,Doremus RH,et al.Enhanced functions of osteoblasts on nanophase ceramics[J].Biomaterials,2000,21(17):1803-1810
[14]Miyauchi T,Yamada M,Yamamoto A,et al.The enhanced characteristics of osteoblast adhesion to photofunctionalized nanoscale Ti O2layers on biomaterials surfaces[J].Biomaterials,2010,31(14):3827-3839
[15]Valerio P,Pereira MM,Goes AM,et al.The effect of ionic products from bioactive glass dissolution on osteoblast proliferation and collagen production[J].Biomaterials,2004,25(15):2941-2948
[16]Silver IA,Deas J,Erecińska M.Interactions of bioactive glasses with osteoblasts in vitro:effects of 45S5 bioglass,and 58S and 77S bioactive glasses on metabolism,intracellular ion concentrations and cell viability[J].Biomaterials,2001,22(2):175-185
[17]Maeno S,Niki Y,Matsumoto H,et al.The effect of calcium ion concentration on osteoblast viability,proliferation and differentiation in monolayer and 3Dculture[J].Biomaterials,2005,26(23):4847-4855
[18]Cerrutti M,Sahai N.Silicate biomaterials for orthopaedic and dental implants[J].Rev Mineral Geochem,2006,64(1):283-313
[19]Nayab SN,Jones FH,Olsen I.Effects of Calcium ion implantation on human bone cell interaction with titanium[J].Biomaterials,2005,26(23):4717-4727
[20]Marom R,Shur I,Solomon R,et al.Characterization of adhesion and differentiation markers of osteogenic marrow stromal cells[J].J Cell Physiol,2005,202(1):41-48