基于纹党参鲜药材的产地加工炮制一体化技术研究
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摘要
目的研究不同干燥过程对纹党参饮片有效成分的影响,确定最佳的基于纹党参鲜药材的产地初加工与炮制一体化工艺。方法对采收期的纹党参鲜药材分别采用3种加工炮制方法:1烘箱80℃烘至含水量为药材原始含水量不同百分比(30%~100%)时,分别取出切片,50℃烘干,制得8个饮片样品(XⅠYP1~8)。2鲜纹党参直接在不同温度(50~120℃)分别烘至含水量为药材原始含水量的50%时,取出切片,50℃烘干,制得8个饮片样品(XⅡYP1~8)。3纹党参干药材,闷润,切片,自然晾干,制得传统饮片。采用HPLC测定各样品中党参炔苷和苍术内酯Ⅲ,苯酚-硫酸法和比色法分别测定多糖及总黄酮含量,并同时测定醇浸出物、水浸出物含量。结果饮片XⅠYP3的醇浸出物(55.36%)、水浸出物(54.91%)和苍术内酯Ⅲ(10.95μg/g)含量均高于其他饮片。结论优选的工艺为:80℃烘至含水量50%,切片,干燥。获得的一体化加工工艺较传统方法省时、省力。制得的饮片活性成分含量高。
Objective To study the effects of different drying processes on the effective constituents in Wen Codonopsis pilosula(WPP) Decoction Pieces;To develop the optimized producing and preparing integration process based on fresh WPP.Methods Fresh WPP in harvest period was prepared respectively as follows:1 Fresh WPP was dried to different percentage(30%–100%) of original moisture contents of crude drugs at 80 ℃ in oven,then sliced and dried at 50 ℃ to obtain eight decoction pieces of WPP(XⅠYP1–8).2 The fresh WPP was baked to 50% of water content of crude drug under different temperatures(50–120 ℃),respectively,then sliced and dried at 50 ℃to obtain eight decoction pieces of WPP(XⅡYP1–8).3 Dried WPP was moistened,sliced and naturally dried,then were renamed as traditional decoction pieces.The contents of lobetyolin and atractylenolide Ⅲ was determined by HPLC.The phenol-sulfuric acid and colorimetric method were applied respectively to detect contents of polysaccharide and total flavonoids.The contents of aqueous and alcoholic extracts were determined simultaneously.Results The contents of alcoholic extracts(55.36%),aqueous extracts(54.91%) and atractylenolide Ⅲ(10.95 μg/g) in XⅠYP3 pieces were higher than other decoction pieces.Conclusion The optimized process was that fresh WPP was baked to water content of 50% at 80 ℃,then sliced and dried.Compared with conventional preparing methods,the integration process was time-saving and effort-saving.Meanwhile,the prepared pieces have higher content of active ingredients.
Objective To study the effects of different drying processes on the effective constituents in Wen Codonopsis pilosula(WPP) Decoction Pieces;To develop the optimized producing and preparing integration process based on fresh WPP.Methods Fresh WPP in harvest period was prepared respectively as follows:1 Fresh WPP was dried to different percentage(30%–100%) of original moisture contents of crude drugs at 80 ℃ in oven,then sliced and dried at 50 ℃ to obtain eight decoction pieces of WPP(XⅠYP1–8).2 The fresh WPP was baked to 50% of water content of crude drug under different temperatures(50–120 ℃),respectively,then sliced and dried at 50 ℃to obtain eight decoction pieces of WPP(XⅡYP1–8).3 Dried WPP was moistened,sliced and naturally dried,then were renamed as traditional decoction pieces.The contents of lobetyolin and atractylenolide Ⅲ was determined by HPLC.The phenol-sulfuric acid and colorimetric method were applied respectively to detect contents of polysaccharide and total flavonoids.The contents of aqueous and alcoholic extracts were determined simultaneously.Results The contents of alcoholic extracts(55.36%),aqueous extracts(54.91%) and atractylenolide Ⅲ(10.95 μg/g) in XⅠYP3 pieces were higher than other decoction pieces.Conclusion The optimized process was that fresh WPP was baked to water content of 50% at 80 ℃,then sliced and dried.Compared with conventional preparing methods,the integration process was time-saving and effort-saving.Meanwhile,the prepared pieces have higher content of active ingredients.
引文
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[8]贺玉林,李先恩,潘广州,等.党参质量变异研究[J].时珍国医国药,2006,17(9):1727-1728.
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[13]谢大泽,湛学军,章涛,等.枸杞等5种多糖清除氧自由基的实验研究[J].江西医学检验,2002,20(4):195-196.
[14]陈嘉屿,胡林海,吴红梅,等.党参多糖类对荷瘤小鼠免疫应答及抑瘤作用研究[J].中华肿瘤防治杂志,2015,22(17):1357-1362.
[15]王敏,王彦春,洪小平,等.党参及其复方对亚急性衰老模型小鼠IL-2影响的研究[J].湖北中医杂志,2004,26(7):6-7.
[16]LIU C,CHEN J,LI E T,et al.The comparison of antioxidative and hepatoprotective activities of Codonopsis pilosula polysaccharide(CP)and sulfated CP[J].Int Immunopharmacol,2015,24(2):299-305.
[17]YANG C X,GOU Y Q,CHEN J Y,et al.Structural characterization and antitumor activity of a pectic polysaccharide from Codonopsis pilosula[J].Carbohydrate Polymers,2013,98(1):886-895.
[18]褚海滨,王玉芳.党参多糖对小鼠抗运动性疲劳作用的研究[J].中国现代医生,2011,49(30):1-2.
[19]张振东,吴兰芳,景永帅,等.党参多糖对小鼠学习记忆作用研究[J].山地农业生物学报,2010,29(3):242-245.
[20]王绍坤,刘荣建.党参和维生素E对大鼠下丘脑室周核生长抑素神经元增龄变化的影响[J].解剖学杂志,1996,19(6):489-493.
[21]李艳,孙萍,刘霞,等.新疆党参多糖的提取、含量分析及免疫功能的初步研究[J].中成药,2005,27(7):839-840.
[22]万利秀,肖更生,徐玉娟,等.甲醇提取柑橘皮总黄酮的工艺优化[J].安徽农业科学,2010,38(27):15087-15089.
[23]冯月,吴文夫,魏建华,等.正交设计优化党参炔苷提取工艺的研究[J].人参研究,2013,25(2):46-48.
[24]赵晓华,刘养清,王润生,等.党参不同部位中党参炔苷的RP-HPLC分析[J].中成药,2007,29(7):1046-1047.
[25]武果桃,贺东昌,张李俊,等.中药党参的HPLC指纹图谱研究[J].中兽医医药杂志,2009,28(5):54-56.
[2]饶伟文,周文杰.中药产地加工规范化研究进展[J].中国中医药信息杂志,2012,19(2):106-109.
[3]王海鹏,韩多,井彩巧,等.对甘肃省党参、当归、黄芪等特产名贵药材的综述[J].中国农业信息,2013(7):68-69.
[4]黄冬兰,陈小康,徐永群,等.纹党参与白条党参红外光谱的SIMCA聚类鉴别方法研究[J].分析测试学报,2009,28(12):1440-1443.
[5]李宝凤.文县纹党的初加工技术规程[J].农业科技与信息,2006(1):30.
[6]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:281.
[7]全世佑.浅析党参的加工储藏技术[J].甘肃农业,2013(12):25-26.
[8]贺玉林,李先恩,潘广州,等.党参质量变异研究[J].时珍国医国药,2006,17(9):1727-1728.
[9]于晓东.党参加工技术研究[J].人参研究,2010,22(2):48.
[10]宋晓燕.党参的研究进展[J].内蒙古中医药,2011,30(8):112-113.
[11]李晓峰.党参的化学成分及药理作用研究概况[J].中国乡村医药,2014,21(21):83-84.
[12]陈克克,王喆之.党参多糖的研究进展[J].现代生物医学进展,2007,7(4):635-637.
[13]谢大泽,湛学军,章涛,等.枸杞等5种多糖清除氧自由基的实验研究[J].江西医学检验,2002,20(4):195-196.
[14]陈嘉屿,胡林海,吴红梅,等.党参多糖类对荷瘤小鼠免疫应答及抑瘤作用研究[J].中华肿瘤防治杂志,2015,22(17):1357-1362.
[15]王敏,王彦春,洪小平,等.党参及其复方对亚急性衰老模型小鼠IL-2影响的研究[J].湖北中医杂志,2004,26(7):6-7.
[16]LIU C,CHEN J,LI E T,et al.The comparison of antioxidative and hepatoprotective activities of Codonopsis pilosula polysaccharide(CP)and sulfated CP[J].Int Immunopharmacol,2015,24(2):299-305.
[17]YANG C X,GOU Y Q,CHEN J Y,et al.Structural characterization and antitumor activity of a pectic polysaccharide from Codonopsis pilosula[J].Carbohydrate Polymers,2013,98(1):886-895.
[18]褚海滨,王玉芳.党参多糖对小鼠抗运动性疲劳作用的研究[J].中国现代医生,2011,49(30):1-2.
[19]张振东,吴兰芳,景永帅,等.党参多糖对小鼠学习记忆作用研究[J].山地农业生物学报,2010,29(3):242-245.
[20]王绍坤,刘荣建.党参和维生素E对大鼠下丘脑室周核生长抑素神经元增龄变化的影响[J].解剖学杂志,1996,19(6):489-493.
[21]李艳,孙萍,刘霞,等.新疆党参多糖的提取、含量分析及免疫功能的初步研究[J].中成药,2005,27(7):839-840.
[22]万利秀,肖更生,徐玉娟,等.甲醇提取柑橘皮总黄酮的工艺优化[J].安徽农业科学,2010,38(27):15087-15089.
[23]冯月,吴文夫,魏建华,等.正交设计优化党参炔苷提取工艺的研究[J].人参研究,2013,25(2):46-48.
[24]赵晓华,刘养清,王润生,等.党参不同部位中党参炔苷的RP-HPLC分析[J].中成药,2007,29(7):1046-1047.
[25]武果桃,贺东昌,张李俊,等.中药党参的HPLC指纹图谱研究[J].中兽医医药杂志,2009,28(5):54-56.