牛舌草多糖的含量测定以及毛细管电泳分析
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摘要
目的:提取牛舌草粗多糖,纯化后测定牛舌草多糖含量并分析其单糖组分。方法:水提醇沉法提取粗多糖,苯酚-硫酸显色法并在491 nm处测定其含量。多糖经Superdex 200纯化后经高效毛细管电泳(HPCE)测定多糖组分。结果:牛舌草中多糖含量为16.38%,葡萄糖在30～180μg·mL~(-1)范围内线性关系良好(r=0.9980),平均加样回收率为99.18%。牛舌草多糖由鼠李糖、木糖、葡萄糖、甘露糖、阿拉伯糖和半乳糖以摩尔比12∶5∶40∶17∶70∶56组成。结论:苯酚-硫酸法实验操作简便,准确,可用于牛舌草多糖含量的测定。毛细管电泳分离度高,可用于牛舌草多糖中单糖组分分析。
Objective: To determine the contents of the polysaccharide in Niushecao(Borage officinales L.and analyze its monosaccharide components. Methods: The contents were determined by phenol-sulfuric acid method at 491 nm, meanwhile, the polysaccharide was separated and purified by Sephadex G, and HPCE was applied to analyze its monosaccharides. Results: The average content of polysaccharide in Niushecao(Borage officinales L.) was 16.38%. There is a good linear equation of the concentration of glucose with absorbance within 30-180 μg·mL~(-1)(r=0.9980), and the average recovery was 99.18%. The polysaccharides were mainly composed of rhamnose, xylose, glucose, mannose, arabinose and galactose, with the molar ratio of 12∶5∶40∶17∶70∶56. Conclusion: The phenol-sulfuric acid method can be easily controlled and operated, and can be applied to determine the contents of polysaccharide in Niushecao(Borage officinales L.). HPCE is a good way to analyze the monosaccharide components of polysaccharides.
Objective: To determine the contents of the polysaccharide in Niushecao(Borage officinales L.and analyze its monosaccharide components. Methods: The contents were determined by phenol-sulfuric acid method at 491 nm, meanwhile, the polysaccharide was separated and purified by Sephadex G, and HPCE was applied to analyze its monosaccharides. Results: The average content of polysaccharide in Niushecao(Borage officinales L.) was 16.38%. There is a good linear equation of the concentration of glucose with absorbance within 30-180 μg·mL~(-1)(r=0.9980), and the average recovery was 99.18%. The polysaccharides were mainly composed of rhamnose, xylose, glucose, mannose, arabinose and galactose, with the molar ratio of 12∶5∶40∶17∶70∶56. Conclusion: The phenol-sulfuric acid method can be easily controlled and operated, and can be applied to determine the contents of polysaccharide in Niushecao(Borage officinales L.). HPCE is a good way to analyze the monosaccharide components of polysaccharides.
引文
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[2]Ketabchi S,Moatari A,Shadram M,et al.The antiinfluenzavirus activity of Anchusa italic[J].Asian J Exp Biol Sci,2011,20(4):558-561.
[3]王超,王一民,郭飞,等.大枣多糖对CFS大鼠的免疫调节作用[J].西北大学学报(自然科学版),2015,45(1):73-75.
[4]张丽梅,程永强,宋曙辉.紫山药多糖对D-半乳糖衰老模型大鼠肝、脑的影响[J].食品科学,2017,38(13):196-200.
[5]胡亚平,张金秀,赵立强,等.橘黄裸伞多糖的理化特性及体外抗氧化活性[J].天然产物研究与开发,2018,30(2):274-279.
[6]刘静.枸杞多糖对小鼠移植性肝癌抑制作用的实验研究[J].中医药导报,2013,19(5):89-91.
[7]马海宁,华玉娟,屠春燕,等.毛细管电泳法分析藏红花植物细胞多糖中单糖组成[J].色谱,2012,30(3):304-308.
[8]杨跃辉,姜清华,丁平田.植物中多糖分析方法的研究进展[J].中草药,2011,42(6):1239-1242.
[9]陈义.毛细管电泳技术及应用[M].北京:化学工业出版社,2006:268.
[10]张晖,宋峰峰,谢睆,等.毛细管区带电泳法分析药桑多糖中单糖的组成[J].新疆医科大学学报,2016,39(8):1017-1025.