不同诱导培养基对糙皮侧耳液体发酵产漆酶活性的影响
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摘要
利用1株糙皮侧耳Pleurotus ostreatus栽培菌株为材料,研究添加碱性木质素或者配合简单碳源或氮源后对其液体发酵产漆酶活性的影响。结果表明,不同诱导培养基对糙皮侧耳漆酶活性具有极显著的影响(P<0.001),而且不同诱导培养基对糙皮侧耳菌丝生物量也产生了极显著的影响(P<0.001)。此外,只利用碱性木质素或者是再添加碳源葡萄糖均有利于糙皮侧耳产漆酶,既包括产漆酶酶活性的提升,同时也包括产漆酶时间的提前,但只利用碱性木质素诱导不利于菌丝生物量的积累;而富含简单碳/氮源的诱导培养基,无论是否含碱性木质素,均有利于菌丝生物量的积累,其中,富含简单碳/氮源的培养基中再添加碱性木质素后的菌丝生物量和漆酶活性均高于不添加碱性木质素时的菌丝生物量和漆酶活性。相比而言,含碱性木质素的培养基中测得的漆酶活性大部分时间下都要高于不含木质素的简单碳/氮源培养基,含碱性木质素的培养基对糙皮侧耳菌株产漆酶的诱导作用更强。
A cultivated Pleurotus ostreatus strain was used to investigate the effects of adding the alkaline lignin or cooperating with simple carbon source or nitrogen source on laccase activities in submerged fermentation.The results demonstrated that the laccase activities secreted by the P.ostreatus from different induction media were significantly different(P<0.001),and the biomass obtained by the P.ostreatus from different induction media were also significantly different(P<0.001).Only using the alkaline lignin or cooperating with simple carbon source glucose was in favour of laccase production of P.ostreatus,increasing laccase activities and advancing enzyme production time.Only using alkaline lignin as inducer is unfavourable to accumulating mycelial biomass.The induction media rich in simple carbon source or nitrogen source,whether containing alkaline lignin,are favourable to accumulating mycelial biomass.The mycelial biomass and laccase activities obtained from the media rich in simple carbon source or nitrogen source with additional alkaline lignin were higher than those obtained from the media only containing simple carbon source or nitrogen source without additional alkaline lignin.In general,the laccase activity in the media containing alkaline lignin was constantly higher than that in simple carbon or nitrogen media without additional alkaline lignin.The inductive effect of the media containing alkaline lignin on laccase production of P.ostreatus was stronger as compared with simple carbon or nitrogen media without additional alkaline lignin.
A cultivated Pleurotus ostreatus strain was used to investigate the effects of adding the alkaline lignin or cooperating with simple carbon source or nitrogen source on laccase activities in submerged fermentation.The results demonstrated that the laccase activities secreted by the P.ostreatus from different induction media were significantly different(P<0.001),and the biomass obtained by the P.ostreatus from different induction media were also significantly different(P<0.001).Only using the alkaline lignin or cooperating with simple carbon source glucose was in favour of laccase production of P.ostreatus,increasing laccase activities and advancing enzyme production time.Only using alkaline lignin as inducer is unfavourable to accumulating mycelial biomass.The induction media rich in simple carbon source or nitrogen source,whether containing alkaline lignin,are favourable to accumulating mycelial biomass.The mycelial biomass and laccase activities obtained from the media rich in simple carbon source or nitrogen source with additional alkaline lignin were higher than those obtained from the media only containing simple carbon source or nitrogen source without additional alkaline lignin.In general,the laccase activity in the media containing alkaline lignin was constantly higher than that in simple carbon or nitrogen media without additional alkaline lignin.The inductive effect of the media containing alkaline lignin on laccase production of P.ostreatus was stronger as compared with simple carbon or nitrogen media without additional alkaline lignin.
引文
An Q,2016.Invesigation on enzymatic activities in different Flammulina strains.Master Thesis,Beijing Forestry University,Beijing.33-51(in Chinese)
An Q,Han ML,Wu XJ,Si J,Cui BK,Dai YC,Wu B,2016a.Laccase production among medicinal mushrooms from the genus Flammulina(Agaricomycetes)under different treatments in submerged fermentation.International Journal of Medicinal Mushrooms,18:1049-1059
An Q,Wu XJ,Han ML,Cui BK,He SH,Dai YC,Si J,2016b.Sequential solid-state and submerged cultivation of the white rot fungus Pleurotus ostreatus on biomass and the activity of lignocellulolytic enzymes.Bioresources,11(4):8791-8805
An Q,Wu XJ,Wu B,Dai YC,2015.Effects of carbon and nitrogen sources on lignocellulose decomposition enzyme activities in Flammulina velutipes.Mycosystema,34 (4):761-771(in Chinese)
Bari E,Nazarnezhad N,Kazemi SM,Ghanbary MAT,Mohebby B,Schmidt O,Clausen CA,2015.Comparison between degradation capabilities of the white rot fungi Pleurotus ostreatus and Trametes versicolor in beech wood.International Biodeterioration&Biodegradation,104:231-237
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Birhanli E,Ye?ilada?,2013.The utilization of lignocellulosic wastes for laccase production under semisolid-state and submerged fermentation conditions.Turkish Journal of Biology,37:450-456
Cohen R,Persky L,Hadar Y,2002.Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus.Applied Microbiology and Biotechnology,58(2):582-594
Couto SR,Herrera JLT,2006.Industrial and biotechnological applications of laccases:a review.Biotechnology Advances,24(5):500-513
Dai YC,Cui BK,Yuan HS,Li BD,2007.Pathogenic wood-decaying fungi in China.Forest Pathology,37(2):105-120
Dai YC,Yang ZL,2008.A revised checklist of medicinal fungi in China.Mycosystema,27(6):801-824(in Chinese)
Dai YC,Zhou LW,Yang ZL,Wen HA,Bau T,Li TH,2010.Arevised checklist of edible fungi in China.Mycosystema,29(1):1-21(in Chinese)
Elisashvili V,Penninckx M,Kachlishvili E,Tsiklauri N,Metreveli E,Kharziani T,Kvesitadze G,2008.Lentinus edodes and Pleurotus species lignocellulolytic enzymes activity in submerged and solid-state fermentation of lignocellulosic wastes of different composition.Bioresource Technology,99(3):457-462
Galhaup C,Haltrich D,2001.Enhanced formation of laccase activity by the white-rot fungus Trametes pubescens in the presence of copper.Applied Microbiology and Biotechnology,56(1-2):225-232
Galhaup C,Wagner H,Hinterstoisser B,Haltrich D,2002.Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens.Enzyme and Microbial Technology,30(4):529-536
Gomes E,Aguiar AP,Carvalho CC,BonfáMRB,da Silva R,Boscolo M,2009.Ligninases production by basidiomycetes strains on lignocellulosic agricultural residues and their application in the decolorization of synthetic dyes.Brazilian Journal of Microbiology,40(1):31-39
Han ML,An Q,Wu XJ,Zheng F,Si J,2017.Effects of different lignocellulose as inducers on laccase activities of Pleurotus ostreatus in submerged fermentation.Mycosystema,36(3):349-357(in Chinese)
He YC,Gong L,Liu F,Lu T,Qing Q,Wang LQ,Zhang Y,Gao FT,Wang X,2014.Waste biogas residue from cassava dregs as carbon source to produce Galactomyces sp.CCZU11-1cellulase and its enzymatic saccharification.Applied Biochemistry and Biotechnology,173(4):894-903
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Hu XK,Zhao XH,Hwang HM,2007.Comparative study of immobilized Trametes versicolor laccase on nanoparticles and kaolinite.Chemosphere,66(9):1618-1626
Isikhuemhen OS,Mikiashvilli NA,2009.Lignocellulolytic enzyme activity,substrate utilization,and mushroom yield by Pleurotus ostreatus cultivated on substrate containing anaerobic digester solids.Journal of Industrial Microbiology&Biotechnology,36(11):1353-1362
Janusz G,Czury?o A,Fr?c M,Rola B,Sulej J,Pawlik A,Siwulski M,Rogalski J,2015.Laccase production and metabolic diversity among Flammulina velutipes strains.World Journal of Microbiology and Biotechnology,31(1):121-133
Kaal EEJ,de Jong ED,Field JA,1993.Stimulation of ligninolytic peroxidase activity by nitrogen nutrients in the white rot fungus Bjerkandera sp.strain BOS55.Applied and Environmental Microbiology,59(12):4031-4036
Levasseur A,Drula E,Lombard V,Coutinho PM,Henrissat B,2013.Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes.Biotechnology for Biofuels,6:41
Li P,Wang HL,Liu GS,Li X,Yao JM,2011.The effect of carbon source succession on laccase activity in the co-culture process of Ganoderma lucidum and a yeast.Enzyme and Microbial Technology,48(1):1-6
Lorenzo M,Moldes D,Couto SR,Sanromán A,2002.Improving laccase production by employing different lignocellulosic wastes in submerged cultures of Trametes versicolor.Bioresource Technology,82(2):109-113
Lundell TK,Makela MR,Hilden K,2010.Lignin-modifying enzymes in filamentous basidiomycetes-ecological,functional and phylogenetic review.Journal of Basic Microbiology,50(1):5-20
MartínezáT,Speranza M,Ruiz-Due?as FJ,Ferreira P,Camarero S,Guillén F,Martínez MJ,Gutiérrez A,del Río JC,2005.Biodegradation of lignocellulosics:microbial,chemical,and enzymatic aspects of the fungal attack of lignin.International Microbiology,8(3):195-204
Mayer AM,Staples RC,2002.Laccase:new functions for an old enzyme.Phytochemistry,60(6):551-565
Membrillo I,Sánchez C,Meneses M,Favela E,Loera O,2008.Effect of substrate particle size and additional nitrogen source on production of lignocellulolytic enzymes by Pleurotus ostreatus strains.Bioresource Technology,99(16):7842-7847
Odier E,Artaud I,1992.Degradation of lignin.In:Winkelmann G(ed.)Microbial degradation of natural products.Wiley-VCH Publishers,Weinheim.161-191
Osma JF,Saravia V,Herrera JLT,Rodríguez Couto S,2007.Mandarin peelings:the best carbon source to produce laccase by static cultures of Trametes pubescens.Chemosphere,67(8):1677-1680
Saito K,Ikeda R,Endo K,Tsujino Y,Takagi M,Tamiya E,2012.Isolation of a novel alkaline-induced laccase from Flammulina velutipes and its application for hair coloring.Journal of Bioscience and Bioengineering,113(5):575-579
Shang J,Wu QX,Lian XL,Wang QY,2013.The effect of carbon and nitrogen sources on laccase production and properties from Fomes fomentarius.China Biotechnology,33(11):32-37(in Chinese)
Si J,Cui BK,Dai YC,2011c.Primary screening of effective Trametes strains with high laccase-productivity and optimization of conditions on laccase production.Microbiology China,38(3):405-416(in Chinese)
Si J,Cui BK,He S,Dai YC,2011b.Optimization of conditions for laccase production by Perenniporia subacida and its application in dye decolorization.Chinese Journal of Applied and Environmental Biology,17(5):736-741(in Chinese)
Si J,Li W,Cui BK,Dai YC,2011a.Advances of research on characteristic,molecular biology and applications of laccase from fungi.Biotechnology Bulletin,223(2):48-55(in Chinese)
Soden DM,Dobson ADW,2003.The use of amplified flanking region-PCR in the isolation of laccase promoter sequences from the edible fungus Pleurotus sajor-caju.Journal of Applied Microbiology,95:553-562
Srinivasan C,D’Souza TM,Boominathan K,Reddy CA,1995.Demonstration of laccase in the white rot basidiomycete Phanerochaete chrysosporium BKM-F1767.Applied and Environmental Microbiology,61(12):4274-4277
Staji?M,Persky L,Friesem D,Hadar Y,Wasser SP,Nevo E,Vukojevi?J,2006.Effect of different carbon and nitrogen sources on laccase and peroxidases production by selected Pleurotus species.Enzyme and Microbial Technology,38(1-2):65-73
Suetomi T,Sakamoto T,Tokunaga Y,Kameyama T,Honda Y,Kamitsuji H,Kameshita I,Izumitsu K,Suzuki K,Irie T,2015.Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus.Current Genetics,61(2):127-140
Sulej J,Janusz G,Osińska-Jaroszuk M,Ma?ek P,Mazur A,Komaniecka I,Choma A,Rogalski J,2013.Characterization of cellobiose dehydrogenase and its FAD-domain from the ligninolytic basidiomycete Pycnoporus sanguineus.Enzyme and Microbial Technology,53(6-7):427-437
Sun X,Zhang RH,Zhang YZ,2004.Production of lignocellulolytic enzymes by Trametes gallica and detection of polysaccharide hydrolase and laccase activities in polyacrylamide gels.Journal of Basic Microbiology,44(3):220-231
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An Q,Wu XJ,Han ML,Cui BK,He SH,Dai YC,Si J,2016b.Sequential solid-state and submerged cultivation of the white rot fungus Pleurotus ostreatus on biomass and the activity of lignocellulolytic enzymes.Bioresources,11(4):8791-8805
An Q,Wu XJ,Wu B,Dai YC,2015.Effects of carbon and nitrogen sources on lignocellulose decomposition enzyme activities in Flammulina velutipes.Mycosystema,34 (4):761-771(in Chinese)
Bari E,Nazarnezhad N,Kazemi SM,Ghanbary MAT,Mohebby B,Schmidt O,Clausen CA,2015.Comparison between degradation capabilities of the white rot fungi Pleurotus ostreatus and Trametes versicolor in beech wood.International Biodeterioration&Biodegradation,104:231-237
Bhattacharya S,Das A,Palaniswamy M,Angayarkanni J,2017.Degradation of benzo[a]pyrene by Pleurotus ostreatus PO-3 in the presence of defined fungal and bacterial co-cultures.Journal of Basic Microbiology,57(2):95-103,doi:10.1002/jobm.201600479
Birhanli E,Ye?ilada?,2013.The utilization of lignocellulosic wastes for laccase production under semisolid-state and submerged fermentation conditions.Turkish Journal of Biology,37:450-456
Cohen R,Persky L,Hadar Y,2002.Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus.Applied Microbiology and Biotechnology,58(2):582-594
Couto SR,Herrera JLT,2006.Industrial and biotechnological applications of laccases:a review.Biotechnology Advances,24(5):500-513
Dai YC,Cui BK,Yuan HS,Li BD,2007.Pathogenic wood-decaying fungi in China.Forest Pathology,37(2):105-120
Dai YC,Yang ZL,2008.A revised checklist of medicinal fungi in China.Mycosystema,27(6):801-824(in Chinese)
Dai YC,Zhou LW,Yang ZL,Wen HA,Bau T,Li TH,2010.Arevised checklist of edible fungi in China.Mycosystema,29(1):1-21(in Chinese)
Elisashvili V,Penninckx M,Kachlishvili E,Tsiklauri N,Metreveli E,Kharziani T,Kvesitadze G,2008.Lentinus edodes and Pleurotus species lignocellulolytic enzymes activity in submerged and solid-state fermentation of lignocellulosic wastes of different composition.Bioresource Technology,99(3):457-462
Galhaup C,Haltrich D,2001.Enhanced formation of laccase activity by the white-rot fungus Trametes pubescens in the presence of copper.Applied Microbiology and Biotechnology,56(1-2):225-232
Galhaup C,Wagner H,Hinterstoisser B,Haltrich D,2002.Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens.Enzyme and Microbial Technology,30(4):529-536
Gomes E,Aguiar AP,Carvalho CC,BonfáMRB,da Silva R,Boscolo M,2009.Ligninases production by basidiomycetes strains on lignocellulosic agricultural residues and their application in the decolorization of synthetic dyes.Brazilian Journal of Microbiology,40(1):31-39
Han ML,An Q,Wu XJ,Zheng F,Si J,2017.Effects of different lignocellulose as inducers on laccase activities of Pleurotus ostreatus in submerged fermentation.Mycosystema,36(3):349-357(in Chinese)
He YC,Gong L,Liu F,Lu T,Qing Q,Wang LQ,Zhang Y,Gao FT,Wang X,2014.Waste biogas residue from cassava dregs as carbon source to produce Galactomyces sp.CCZU11-1cellulase and its enzymatic saccharification.Applied Biochemistry and Biotechnology,173(4):894-903
Hou HM,Zhou JT,Wang J,Du CH,Yan B,2004.Enhancement of laccase production by Pleurotus ostreatus and its use for the decolorization of anthraquinone dye.Process Biochemistry,39(11):1415-1419
Hu XK,Zhao XH,Hwang HM,2007.Comparative study of immobilized Trametes versicolor laccase on nanoparticles and kaolinite.Chemosphere,66(9):1618-1626
Isikhuemhen OS,Mikiashvilli NA,2009.Lignocellulolytic enzyme activity,substrate utilization,and mushroom yield by Pleurotus ostreatus cultivated on substrate containing anaerobic digester solids.Journal of Industrial Microbiology&Biotechnology,36(11):1353-1362
Janusz G,Czury?o A,Fr?c M,Rola B,Sulej J,Pawlik A,Siwulski M,Rogalski J,2015.Laccase production and metabolic diversity among Flammulina velutipes strains.World Journal of Microbiology and Biotechnology,31(1):121-133
Kaal EEJ,de Jong ED,Field JA,1993.Stimulation of ligninolytic peroxidase activity by nitrogen nutrients in the white rot fungus Bjerkandera sp.strain BOS55.Applied and Environmental Microbiology,59(12):4031-4036
Levasseur A,Drula E,Lombard V,Coutinho PM,Henrissat B,2013.Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes.Biotechnology for Biofuels,6:41
Li P,Wang HL,Liu GS,Li X,Yao JM,2011.The effect of carbon source succession on laccase activity in the co-culture process of Ganoderma lucidum and a yeast.Enzyme and Microbial Technology,48(1):1-6
Lorenzo M,Moldes D,Couto SR,Sanromán A,2002.Improving laccase production by employing different lignocellulosic wastes in submerged cultures of Trametes versicolor.Bioresource Technology,82(2):109-113
Lundell TK,Makela MR,Hilden K,2010.Lignin-modifying enzymes in filamentous basidiomycetes-ecological,functional and phylogenetic review.Journal of Basic Microbiology,50(1):5-20
MartínezáT,Speranza M,Ruiz-Due?as FJ,Ferreira P,Camarero S,Guillén F,Martínez MJ,Gutiérrez A,del Río JC,2005.Biodegradation of lignocellulosics:microbial,chemical,and enzymatic aspects of the fungal attack of lignin.International Microbiology,8(3):195-204
Mayer AM,Staples RC,2002.Laccase:new functions for an old enzyme.Phytochemistry,60(6):551-565
Membrillo I,Sánchez C,Meneses M,Favela E,Loera O,2008.Effect of substrate particle size and additional nitrogen source on production of lignocellulolytic enzymes by Pleurotus ostreatus strains.Bioresource Technology,99(16):7842-7847
Odier E,Artaud I,1992.Degradation of lignin.In:Winkelmann G(ed.)Microbial degradation of natural products.Wiley-VCH Publishers,Weinheim.161-191
Osma JF,Saravia V,Herrera JLT,Rodríguez Couto S,2007.Mandarin peelings:the best carbon source to produce laccase by static cultures of Trametes pubescens.Chemosphere,67(8):1677-1680
Saito K,Ikeda R,Endo K,Tsujino Y,Takagi M,Tamiya E,2012.Isolation of a novel alkaline-induced laccase from Flammulina velutipes and its application for hair coloring.Journal of Bioscience and Bioengineering,113(5):575-579
Shang J,Wu QX,Lian XL,Wang QY,2013.The effect of carbon and nitrogen sources on laccase production and properties from Fomes fomentarius.China Biotechnology,33(11):32-37(in Chinese)
Si J,Cui BK,Dai YC,2011c.Primary screening of effective Trametes strains with high laccase-productivity and optimization of conditions on laccase production.Microbiology China,38(3):405-416(in Chinese)
Si J,Cui BK,He S,Dai YC,2011b.Optimization of conditions for laccase production by Perenniporia subacida and its application in dye decolorization.Chinese Journal of Applied and Environmental Biology,17(5):736-741(in Chinese)
Si J,Li W,Cui BK,Dai YC,2011a.Advances of research on characteristic,molecular biology and applications of laccase from fungi.Biotechnology Bulletin,223(2):48-55(in Chinese)
Soden DM,Dobson ADW,2003.The use of amplified flanking region-PCR in the isolation of laccase promoter sequences from the edible fungus Pleurotus sajor-caju.Journal of Applied Microbiology,95:553-562
Srinivasan C,D’Souza TM,Boominathan K,Reddy CA,1995.Demonstration of laccase in the white rot basidiomycete Phanerochaete chrysosporium BKM-F1767.Applied and Environmental Microbiology,61(12):4274-4277
Staji?M,Persky L,Friesem D,Hadar Y,Wasser SP,Nevo E,Vukojevi?J,2006.Effect of different carbon and nitrogen sources on laccase and peroxidases production by selected Pleurotus species.Enzyme and Microbial Technology,38(1-2):65-73
Suetomi T,Sakamoto T,Tokunaga Y,Kameyama T,Honda Y,Kamitsuji H,Kameshita I,Izumitsu K,Suzuki K,Irie T,2015.Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus.Current Genetics,61(2):127-140
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