基于纳米金星的层析试纸条用于检测HIV-DNA（英文）
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摘要
构建了基于纳米金星(AuNSs)的快速、简单且可视化的横向流层析试纸条(LFTS),并用于检测人类免疫缺陷病毒的DNA。采用一步法合成AuNSs,并对其进行生物功能化,目标物与DNA修饰的AuNSs结合。该复合物通过碱基互补配对原则被捕获在测试线上,依据测试线上纳米金星颜色的变化进行定性和半定量分析,使用便携式读条器在最佳实验条件下进行定量分析。该方法的线性范围为0.2～50 nmol/L,检出限为0.14 nmol/L。相同条件下,该方法比传统纳米金试纸条的灵敏度约高5倍。该方法可用于人血清中HIV DNA的检测,结果良好。
A rapid,simple and visual lateral flow test strip( LFTS) based on gold nanostars( AuNSs)was fabricated for the detection of human immunodeficiency virus DNA( HIV-DNA) in this paper.The AuNSs as a color label for visual detection were synthesized by an environmentally friendly chemical method. The"sandwich structure"was utilized in the measurement process: the target was firstly captured with the DNA-modified Au NSs,then trapped on the test line in accordance to the principle of complementary base pairing. Afterwards,the variation of the color on test line could be visually observed for qualitative and semi-quantitative analyses,and a portable strip reader recording the color intensity of the band was used for quantitative analysis. Under the optimum conditions,this AuNSsbased LFTS strategy presented a good linear relationship for target DNA in the range of 0. 2-50 nmol/L with a detection limit of 0. 14 nmol/L,which sensitivity was 5-fold higher than that of the traditionanl gold nanoparticles test strip. Moreover,this LFTS exhibited a good anti-interference ability in the detection of HIV DNA in human serum,which meant it has a great potential in early diagnosis and treatment.
A rapid,simple and visual lateral flow test strip( LFTS) based on gold nanostars( AuNSs)was fabricated for the detection of human immunodeficiency virus DNA( HIV-DNA) in this paper.The AuNSs as a color label for visual detection were synthesized by an environmentally friendly chemical method. The"sandwich structure"was utilized in the measurement process: the target was firstly captured with the DNA-modified Au NSs,then trapped on the test line in accordance to the principle of complementary base pairing. Afterwards,the variation of the color on test line could be visually observed for qualitative and semi-quantitative analyses,and a portable strip reader recording the color intensity of the band was used for quantitative analysis. Under the optimum conditions,this AuNSsbased LFTS strategy presented a good linear relationship for target DNA in the range of 0. 2-50 nmol/L with a detection limit of 0. 14 nmol/L,which sensitivity was 5-fold higher than that of the traditionanl gold nanoparticles test strip. Moreover,this LFTS exhibited a good anti-interference ability in the detection of HIV DNA in human serum,which meant it has a great potential in early diagnosis and treatment.
引文
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