一种低毒透明剂制作植物叶片石蜡切片的方法
|
摘要
为探索一种更为安全有效的石蜡切片方法,以禾本科(Gramineae)植物狗牙根(Cynodon dactylon)叶片为试验材料,对石蜡切片的主要技术环节进行改良。用环保产品van-clear代替二甲苯作为透明与脱蜡剂,同时用番红与固绿溶液相结合进行双重染色。试验结果表明,该方法制片效果较好,节省试验时间与人力。van-clear剂可作为一种相对安全有效的二甲苯替代物,应用在植物组织切片中。
In order to explore a safer and more effective paraffin section method,an improvement to preparation of paraffin section was made with leaves of Cynodon dactylon(Gramineae) as the material.Van-clear,an environment-friendly product,was used in place of xylene as transparent and dewaxing agent to remove paraffin wax,and safranin and fast green were used for double staining in the preparation of paraffin sections.Results showed that this improved method had a good effect on the quality of paraffin section,and saved time and manpower.Van-clear can be used as a relatively safe and effective substitute for xylene in the plant-tissue paraffin section.
In order to explore a safer and more effective paraffin section method,an improvement to preparation of paraffin section was made with leaves of Cynodon dactylon(Gramineae) as the material.Van-clear,an environment-friendly product,was used in place of xylene as transparent and dewaxing agent to remove paraffin wax,and safranin and fast green were used for double staining in the preparation of paraffin sections.Results showed that this improved method had a good effect on the quality of paraffin section,and saved time and manpower.Van-clear can be used as a relatively safe and effective substitute for xylene in the plant-tissue paraffin section.
引文
[1]路健,王伶.石蜡切片制作中的问题与解决[J].基础医学教育, 2001, 3(3):263.
[2]李海,李福祥,沈谧.组织制片方法的改进及体会[J].中国基层医药, 2001, 8(2):150.
[3]李正理.植物组织制片学[M].北京:北京大学出版社,1996:88-138.
[4]李玲,王文东,栗学军,等.低浓度苯和甲苯及二甲苯对作业人员健康及血清中超氧化物歧化酶的影响[J].工业卫生和职业病, 2003, 29(4):238.
[5]刘志敏,王春华,刘志勇,等.苯、甲苯和二甲苯作业女工对月经及生殖结局的影响[J].天津医学院学报,1994, 18(3):3-5.
[6]杨虎彪,李晓霞,罗丽娟.植物石蜡制片中透明和脱蜡技术的改良[J].植物学报, 2009, 44(2):230-235.
[7]李素坤,张秋芝,郝玉兰,等.玉米成熟期茎秆石蜡切片方法的研究[J].安徽农业科学, 2010, 38(8):3935-3 937.
[8]杨捷频.常规石蜡切片方法的改良[J].生物学杂志,2006, 23(l):45-46.
[9]汤鸿,刘正华,王利军,等.石蜡切片的标本固定与石蜡精练对制片的影响[J].苏州大学学报(医学版),2002, 22(2):230.
[10]张玉英.制作石蜡切片易出现的问题及改进措施[J].中国科技信息, 2007(6):91-92.
[11]廖秋萍,石长青,饶丽娟.石蜡切片制片技术的探讨[J].塔里木大学学报, 2006, 18(l):69-71.
[2]李海,李福祥,沈谧.组织制片方法的改进及体会[J].中国基层医药, 2001, 8(2):150.
[3]李正理.植物组织制片学[M].北京:北京大学出版社,1996:88-138.
[4]李玲,王文东,栗学军,等.低浓度苯和甲苯及二甲苯对作业人员健康及血清中超氧化物歧化酶的影响[J].工业卫生和职业病, 2003, 29(4):238.
[5]刘志敏,王春华,刘志勇,等.苯、甲苯和二甲苯作业女工对月经及生殖结局的影响[J].天津医学院学报,1994, 18(3):3-5.
[6]杨虎彪,李晓霞,罗丽娟.植物石蜡制片中透明和脱蜡技术的改良[J].植物学报, 2009, 44(2):230-235.
[7]李素坤,张秋芝,郝玉兰,等.玉米成熟期茎秆石蜡切片方法的研究[J].安徽农业科学, 2010, 38(8):3935-3 937.
[8]杨捷频.常规石蜡切片方法的改良[J].生物学杂志,2006, 23(l):45-46.
[9]汤鸿,刘正华,王利军,等.石蜡切片的标本固定与石蜡精练对制片的影响[J].苏州大学学报(医学版),2002, 22(2):230.
[10]张玉英.制作石蜡切片易出现的问题及改进措施[J].中国科技信息, 2007(6):91-92.
[11]廖秋萍,石长青,饶丽娟.石蜡切片制片技术的探讨[J].塔里木大学学报, 2006, 18(l):69-71.