摘要
采用SF 9昆虫细胞-杆状病毒系统重组表达牛传染性鼻气管炎病毒gD蛋白抗原,经纯化鉴定后将gD蛋白用胶体金标记作为示踪抗原,未标记的gD抗原作为捕获抗原,并以羊抗牛IgG抗体作为质控抗体,建立了牛传染性鼻气管炎病毒双抗原夹心法胶体金检测试纸条。试验结果表明,该试纸条检测灵敏度高,特异性良好,与牛口蹄疫病毒、牛病毒性腹泻病毒等阳性血清无交叉反应。使用建立的胶体金试纸条和IDEXX牛传染性鼻气管炎病毒抗体ELISA检测试剂盒同时检测112份牛血清,阳性符合率为93.5%,阴性符合率为96.0%,总符合率为94.6%。说明该试纸条可以应用于临床牛传染性鼻气管炎病毒抗体的诊断和检测。
gD protein antigen of infectious bovine rhinotracheitis virus was prepared by constructing the recombinant expression with sf9 insect cell-baculovirus system, and the purified gD protien was labeled as a tracer by colloidal gold. The colloidal gold test strip of detecting the antibody of infectious bovine rhinotracheitis virus was established with gD protein as a captured antigen, and with the sheep-anti-bovine antibody as a quality control antibody. The results showed that this strip was with high sensitivity, strong specificity, and no cross-reactivity towards bovine foot-and-mouth disease virus and bovine viral diarrhea virus. 112 bovine serum samples were detected by the strip and ELISA(IDEXX-IBRV Antibody Detection Kit) was applied as the comparative method, the coincidence rate of positive results was 93.6%, the coincidence rate of negative results was 96.0%, and the total coincidence rate was 94.6%. This strip could be used for the diagnosis and antibody detection of infectious bovine rhinotracheitis virus.
引文
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