NET-1在结肠癌中的表达、临床意义和预后分析
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摘要
目的探讨新四极体-1(NET-1)在结肠癌中的表达、临床意义及预后分析。方法收集50例结肠癌组织和50例癌旁正常组织,应用逆转录聚合酶链反应法(RT-PCR)和Western blot检测两组中NET-1 mRNA和蛋白质的表达情况,并应用免疫组化法(SP)检测NET-1在两组中的表达,分析NET-1在结肠肿瘤发生、发展中的作用,并随访、分析NET-1的表达与结肠癌患者生存的相关性。结果 RT-PCR结果显示NET-1 mRNA在结肠癌和癌旁正常组织中的表达水平分别为(0.95±0.07)和(0.26±0.04),差异有统计学意义(P=0.005);Western blot结果显示:NET-1蛋白在结肠癌和癌旁正常组织中的表达水平分别为(0.83±0.09)(0.22±0.03),差异有统计学意义(P=0.003);SP法结果表明NET-1蛋白在结肠癌和癌旁正常组织中的阳性表达分别为64.00%(32/50)和36.00%(18/50),差异有统计学意义(P<0.001);NET-1与肿瘤的TNM分期、脉管浸润、淋巴结转移及肝转移等病理参数密切相关;NET-1阳性组的中位生存时间为299 d(95%CI:230.07～303.93),阴性组的中位生存时间为387 d(95%CI:279.16～335.82),差异有统计学意义(P=0.031)。NET-1表达水平与结肠癌患者预后明显相关(RR=2.092,P=0.002)。结论 NET-1的异常表达在结肠癌病变的发生、发展中具有重要作用,且与患者的生存期亦明显相关,是结肠癌潜在的生物学指标。
Objective To study the expression of inhibitors of NET-1 in human colorectal adenocarcinoma tissues and explore their correlation between the expression of NET-1 and clinical prognosis.Methods 50 cases of colorectal adenocarcinoma tissues and 50 cases of normal eolorectal tissues were selected.Reverse-transcription polymerase chain reaction and Western blot were used to detect the expression level of NET-1 mRNA and protein.Meanwhile,NET-1 protein was detected by immunohistochemistry in order to analyze the role in the development and development of colorectal tumor.By follow-up,the correlation between the expression of NET-1 and survival time was ana-lyzed.Results The expression level of NET-1 mRNA in colorectal adenocarcinoma tissues and normal colorectal tissues was(0.95±0.07) and(0.26±0.04),and there was significant difference(P=0.000).The expression of NET-1 protein in colorectal adenocarcinoma tissues and normal colorectal tissues were(0.83±0.09) and(0.22±0.03),and there was significant difference(P=0.000).The positive expression rate of NET-1 protein in colorectal 46 adenocarcinoma tissues(64.00%) was higher than that(36.00%) in normal colorectal tissues(P=0.000).Expression of NET-1 was correlated with TNM stage,lymph node metastasis,vessel invasion,and liver metastasis( P< 0. 05). The median survival time in positive group and negative group were 299 days( 95% CI: 230. 07 ～303. 93) and 387 days( 95% CI: 279. 16 ～ 335. 82),P = 0. 031. The expression of NET-1 was obviously correlatd with the prognosis of colorectal cancer tissues( RR = 2. 092,P = 0. 002). Conclusion NET-1 plays an important role in the occurrence and development of colorectal cancer and is also significantly associated with the survival time,so it is a potential biological indicator of colorectal cancer.
Objective To study the expression of inhibitors of NET-1 in human colorectal adenocarcinoma tissues and explore their correlation between the expression of NET-1 and clinical prognosis.Methods 50 cases of colorectal adenocarcinoma tissues and 50 cases of normal eolorectal tissues were selected.Reverse-transcription polymerase chain reaction and Western blot were used to detect the expression level of NET-1 mRNA and protein.Meanwhile,NET-1 protein was detected by immunohistochemistry in order to analyze the role in the development and development of colorectal tumor.By follow-up,the correlation between the expression of NET-1 and survival time was ana-lyzed.Results The expression level of NET-1 mRNA in colorectal adenocarcinoma tissues and normal colorectal tissues was(0.95±0.07) and(0.26±0.04),and there was significant difference(P=0.000).The expression of NET-1 protein in colorectal adenocarcinoma tissues and normal colorectal tissues were(0.83±0.09) and(0.22±0.03),and there was significant difference(P=0.000).The positive expression rate of NET-1 protein in colorectal 46 adenocarcinoma tissues(64.00%) was higher than that(36.00%) in normal colorectal tissues(P=0.000).Expression of NET-1 was correlated with TNM stage,lymph node metastasis,vessel invasion,and liver metastasis( P< 0. 05). The median survival time in positive group and negative group were 299 days( 95% CI: 230. 07 ～303. 93) and 387 days( 95% CI: 279. 16 ～ 335. 82),P = 0. 031. The expression of NET-1 was obviously correlatd with the prognosis of colorectal cancer tissues( RR = 2. 092,P = 0. 002). Conclusion NET-1 plays an important role in the occurrence and development of colorectal cancer and is also significantly associated with the survival time,so it is a potential biological indicator of colorectal cancer.
引文
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[11] Wang,G L,Chen i,Wei Y Z,et al.The effect of NET-1 on the proliferation,migration and endocytosis of the SMMC-7721 HCC cell line[J].Oncol Rep,2012,27(6):1944-52.
[12] Wu Y Y,Chen L,Wang G L,et al.Inhibition of hepatocellular carcinoma growth and angiogenesis by dual silencing of NET-1 and VEGF[J].J Mol Histol,2013,44(4):433-45.
[13] 宋晓花,耿佃忠,尹洪花,等.NET-1在子宫内膜癌中的表达及意义[J].中国医师杂志,2012,14(1):27-33.
[2] 冯雅靖,王宁,方利文,等.1990年与2013年中国人群结直肠癌疾病负担分析[J].中华流行病学杂志,2016,37(6):768-72.
[3] 宋曙,费嘉鸿,卞伟钢,等.非小细胞肺癌组织中 PGSK-3β、TCF-4、NET-1的表达及意义[J].山东医药,2014,54(29):30-1.
[4] 栗一帆,王晓娟,马晋峰,等.胃腺癌组织中NET-1、FHIT及Ki-67的表达及其临床意义[J].中国肿瘤外科杂志,2013,5(1):26-8.
[5] 叶蕴瑶,陈莉,王桂兰,等.肝细胞肝癌中神经上皮细胞转化基因1及皮质肌动蛋白表达及其意义[J].江苏医药,2015,41(17):2009-13.
[6] Hou F Q,Lei X F,Yao J L,et al.Tetraspanin 1 is involved in survival proliferation and carcinogenesis of pancreatic cancer[J].0ncol Rep,2015,34(6):3068-76.
[7] 郭飞,林杨,张鹏程,等.结直肠癌组织中 CEACAM-1、NET-1 的表达变化及其意义[J].山东医药,2018,58(23):48-50.
[8] 顾婷婷,陈莉.肿瘤相关基因NET-1的研究进展[J].现代肿瘤医学,2016,24(12):1999-2001.
[9] 陈静静,陈建民,孙泉.多肿瘤标志物蛋白芯片检测系统在直结肠癌诊断中的临床意义[J].现代免疫学,2016(4):319-22.
[10] 吴彩兰.四种肿瘤标记物在直结肠癌诊断中的临床应用[J].中国临床研究,2009,22(2):192-3.
[11] Wang,G L,Chen i,Wei Y Z,et al.The effect of NET-1 on the proliferation,migration and endocytosis of the SMMC-7721 HCC cell line[J].Oncol Rep,2012,27(6):1944-52.
[12] Wu Y Y,Chen L,Wang G L,et al.Inhibition of hepatocellular carcinoma growth and angiogenesis by dual silencing of NET-1 and VEGF[J].J Mol Histol,2013,44(4):433-45.
[13] 宋晓花,耿佃忠,尹洪花,等.NET-1在子宫内膜癌中的表达及意义[J].中国医师杂志,2012,14(1):27-33.