缺硼和低pH对枳苗根系细胞壁组分及细胞中硼分布的影响
|
摘要
采用营养液培养的方法,设置pH值为4、5、6,硼浓度为0(缺硼)和10μmol·L~(-1)(适宜硼),探究营养液低pH与柑橘砧木枳[Poncirus trifoliate(L)Raf.]幼苗根细胞壁组分及各组分中硼含量的关系。结果表明:①缺硼和低pH值均抑制根系生长;硼适宜时pH 4处理的根系长度比pH 5和pH 6处理减少了26%和29%,但根系干物质量变化不显著;缺硼时pH 4处理的根系干物质量也显著减少。②硼适宜时pH 4处理的地下部硼含量显著增加,且原生质体硼和游离态硼占根硼的比例均降低;缺硼时pH 4处理的原生质体硼占根硼的比例增加,游离态硼占根硼的比例没有显著变化。③缺硼时,pH 4处理细胞壁提取率显著低于pH 6处理。④不论适宜硼或缺硼,pH 4处理均显著降低根系细胞壁中螯合态果胶含量;适宜硼时碱溶性果胶含量pH 4和pH 5处理分别比pH 6减少了26%和18%;缺硼时pH 4处理比pH 5和pH 6处理减少了约20%。⑤硼适宜时根系纤维素含量随pH值降低而降低,pH 4处理比pH 6处理降低了13.8%;缺硼时低pH对根系纤维素含量影响不大。⑥大部分硼都结合在果胶中,且碱溶性果胶中的硼(Alk-B)的比例大于螯合态果胶(Chel-B)中的。以上结果说明,pH值会通过影响根系细胞壁组分及组分中硼含量而影响根系的伸长生长。
In order to explore changes of cell wall components and boron distribution under low pH in trifoliate root,the different pHs(4,5,6)and boron concentrations(0 and 10 μmol · L~(-1))were set up in experiment. The results showed that boron deficiency and low pH could significantly inhibit root growth and elongation. When the boron concentration was suitable,the root length of pH 4 decreased by 26% and 29% compared with that of pH 5 and pH 6. When boron was absent,root dry weight of pH 4 was significantly reduced. However,when adding boron,the dry matter increase of pH 4 was not significant. When boron was enough,the boron content in the underground part of pH 4 increased significantly;compared with pH 6,the proportion of boron in protoplast and free boron to root boron in pH 4 reduced. Compared with pH 6,the proportion of boron in protoplasts to root boron treated by pH 4 increased and the proportion of free boron to root boron was not significantly changed under boron deficiency. Compared with pH 6,the cell wall extraction rate of pH 4 decreased when boron deficiency. Regardless of boron adequate or boron deficiency,pH 4 significantly decreased the content of chelate pectin in the cell wall of the roots of trifoliate. In the suitable boron treatment,the content of alkali soluble pectin treated by pH 4 and pH 5 decreased by 26% and 18% respectively than that of pH 6. The content of alkali soluble pectin treated by pH 4 decreased by about 20% than that of pH 6 and pH 5 treatments under boron deficiency. In addition,when boron deficiency,low pH had little effect on cellulose content in the root of trifoliate. However,the content of cellulose decreased with the decrease of pH value in suitable boron. Compared with pH 6,the cellulose content of pH 4 decreased by 13.8%. Most of the boron was bound to pectin,and the proportion of boron(Alk-B)in alkali soluble pectin was greater than that of chelated pectin(Chel-B). Obviously,low pH can inhibit the root elongation by affecting the content of boron and components of the root cell wall.
In order to explore changes of cell wall components and boron distribution under low pH in trifoliate root,the different pHs(4,5,6)and boron concentrations(0 and 10 μmol · L~(-1))were set up in experiment. The results showed that boron deficiency and low pH could significantly inhibit root growth and elongation. When the boron concentration was suitable,the root length of pH 4 decreased by 26% and 29% compared with that of pH 5 and pH 6. When boron was absent,root dry weight of pH 4 was significantly reduced. However,when adding boron,the dry matter increase of pH 4 was not significant. When boron was enough,the boron content in the underground part of pH 4 increased significantly;compared with pH 6,the proportion of boron in protoplast and free boron to root boron in pH 4 reduced. Compared with pH 6,the proportion of boron in protoplasts to root boron treated by pH 4 increased and the proportion of free boron to root boron was not significantly changed under boron deficiency. Compared with pH 6,the cell wall extraction rate of pH 4 decreased when boron deficiency. Regardless of boron adequate or boron deficiency,pH 4 significantly decreased the content of chelate pectin in the cell wall of the roots of trifoliate. In the suitable boron treatment,the content of alkali soluble pectin treated by pH 4 and pH 5 decreased by 26% and 18% respectively than that of pH 6. The content of alkali soluble pectin treated by pH 4 decreased by about 20% than that of pH 6 and pH 5 treatments under boron deficiency. In addition,when boron deficiency,low pH had little effect on cellulose content in the root of trifoliate. However,the content of cellulose decreased with the decrease of pH value in suitable boron. Compared with pH 6,the cellulose content of pH 4 decreased by 13.8%. Most of the boron was bound to pectin,and the proportion of boron(Alk-B)in alkali soluble pectin was greater than that of chelated pectin(Chel-B). Obviously,low pH can inhibit the root elongation by affecting the content of boron and components of the root cell wall.
引文
Anugoolprasert O,Kinoshita S,Naito H,Shimizu M,Ehara H.1981.Effect of low p H on the growth,physiological characteristics and nutrient absorption of sago palm in a hydroponic system.Journal of Plant Nutrition,4(3):247-260.
Arduini I,Kettner C,Godbold D L,Onnis A,Stefani A.1998.The influence of p H on root morphology and mineral content of Pinus pinaster Ait.seedlings.Plant Biosystems,132(1):3-9.
Arnon T I,Achdout H,Lieberman N,Gazit R,Gonen-Gross T,Katz G,Bar-Llan A,Bloushtain N,Lev M,Joseph A,Kedar E,Porqador A,Mandelboim O.2004.The mechanisms controlling the recognition of tumor-and virus-infected cells by NKp46.Blood,103(2):664.
Bai Ling,Zhou Yun,Zhang Xiao-ran,Song Chun-peng,Cao Ming-qing.2007.Hydrogen peroxide participates in the morphological development of Arabidopsis root regulated by abscisic acid.Chinese Science Bulletin,52(5):608-611.(in Chinese)白玲,周云,张晓然,宋纯鹏,曹鸣庆.2007.过氧化氢参与了脱落酸调控的拟南芥根形态发育.科学通报,52(5):608-611.
Bramley H,Turner N C,Turner D W,Tyerman S D.2010.The contrasting influence of short-term hypoxia on the hydraulic properties of cells and roots of wheat and lupin.Functional Plant Biology,37(3):183-193.
Dell B,Huang L.1997.Physiolgical response of plants to low boron.Plant Soil,193:103-120.
Dimitra C,David J M,Stephen C F.2014.Boron bridging of rhamnogalacturonan-Ⅱ,monitored by gel electrophoresis,occurs during polysaccharide synthesis and secretion but not post-secretion.The Plant Journal,77:534-546.
Ding Wei-hua,Wang Bin,Zhang Ying-hua,Zhou Shun-li,Wang Zhi-min.2012.Effects of p H buffers on the activities of antioxidant enzymes in winter wheat seedlings under different water status.Journal of Wheat Crop,32(5):890-894.(in Chinese)丁位华,王彬,张英华,周顺利,王志敏.2012.p H缓冲液对不同水分状态下冬小麦幼苗抗氧化酶活性的影响.麦类作物学报,32(5):890-894.
Gao Y L,Kong W L,Li B,NI Y,Yuan T,Guo L,Lin H,Fan H,Fan Y,Zhang X.2018.Fabrication and characterization of collagen-based injectable and self-crosslinkable hydrogels for cell encapsulation.Colloids&Surfaces B Biointerfaces,167:448-456.
Gijsman A J.1990.Nitrogen nutrition of Douglas-fir(Pseudotsuga menziesii)on strongly acid sandy soil.II.Proton excretion and rhizosphere p H.Plant&Soil,126(1):53-61.
Han Jia,Zhou Gao-feng,Li Qiao-hong,Liu Yong-zhong,Peng Shu-ang.2012.Effects of magnesium,iron,boron deficiency on the growth and nutrition absorption of four major citrus rootstocks.Acta Horticulturae Sinica,39(11):2105-2112.(in Chinese)韩佳,周高峰,李峤虹,刘永忠,彭抒昂.2012.缺镁、铁、硼胁迫对4个柑橘砧木生长及养分吸收的影响.园艺学报,39(11):2105-2112.
Hipps N A,Davies M J,Dodds P,Buckley G P.2005.The effects of phosphorus nutrition and soil p H on the growth of some ancient woodland indicator plants and their interaction with competitor species.Plant&Soil,271(1-2):131-141.
Hoagland R.1950.The water culture methods for growing plants without soil//California Agricultural Experiment Station Circular:347.
Hoson T.1998.Apoplast as the site of response to environmental signals.Journal of Plant Research,111:167-177.
Hu H,Brown P H,Labavitch J M.1996.Species variability in boron requirement is correlated with cell wall pectin.Journal of Experimental Botany,47:227-232.
Huang Y M,Zou Y N,Wu Q S.2017.Alleviation of drought stress by mycorrhizas is related to increased root H2O2 efflux in trifoliate orange.Scientific Reports,7:42335.
Ishii T,Matsunaga T,Hayashi N.2001.Formation of rhamnogalacturonanⅡ-borate dimer in pectin determines cell wall thickness of pumpkin tissue.Plant Physiology,126:1698-1705.
Ivanchenko M G,Os D D,Monshausen G B,Dubrovsky J G,Bednarova A,Krishnan N.2013.Auxin increases the hydrogen peroxide(H2O2)concentration in tomato(Solanum lycopersicum)root tips while inhibiting root growth.Annals of Botany,112(6):1107-1116.
Kaneko S,Ishii T,Matsunaga T.1997.A boron-rhamnogalacturonan-Ⅱcomplex from bamboo shoot cell wall.Phytochemistry,44:243-248.
Kobayashi M,Matoh T,Azuma J.1996.Two chains of rhamnoglacturonanⅡare cross-linked by borate-diol ester bonds in higher plant cell wall.Plant Physiology,110:1017-1020.
Li Xue-wen,Li Ya-lin,Yang Jin,Wu Li-shu,Xiao Hong-dong,Feng Ying-ming,Liu Jia-you,Yu Min.2016.Differences of pectin content and methylation in root cell wall of pea(Pisum sativum)cultivars with different Al tolerance.Journal of Plant Nutrition and Fertilizer,22(3):729-735.(in Chinese)李学文,李亚林,杨锦,吴礼树,萧洪东,冯英明,刘家友,喻敏.2016.豌豆不同耐铝品种根尖细胞壁果胶及其甲基酯化度的差异.植物营养与肥料学报,22(3):729-735.
Liang Mei-qing,Xue Jun,Fan Yu-lan,Li Xun,Peng Liang-zhi.2010.Studies on soil acidification of navel orange orchards in Gan-zhou City,Jiangxi Province.South China Fruits,39(4):6-8,13.(in Chinese)梁梅青,薛珺,范玉兰,李勋,彭良志.2010.赣南脐橙园土壤酸化特征研究.中国南方果树,39(4):6-8,13.
Liao Wei-biao,Huang Gao-bao,Yu Ji-hua,Zhang Mei-ling.2011.Nitric oxide and hydrogen peroxide are involved in indole-3-butyric acid-induced adventitious root formation of marigold.Acta Horticulturae Sinica,38(5):939-946.(in Chinese)廖伟彪,黄高宝,郁继华,张美玲.2011.NO和H2O2在IBA诱导万寿菊不定根形成中的作用.园艺学报,38(5):939-946.
Liu Guidong,Jiang Cuncang,Wang Yunhua.2011.Distribution of boron and its forms in young“Newhall”navel orange(Citrus sinensis Osb.)plants grafted on two rootstocks in response to deficient and excessive boron.Soil Science&Plant Nutrition,57(1):93-104.
Liu Lei-chao,Jiang Cun-cang,Liu Gui-dong,Dong Xiao-chang,Wu Xiu-wen.2015.Effects of boron stress on seedling growth and nutrition physiology of navel orange root stock.Huazhong Agricultural University,34(3):64-68.(in Chinese)刘磊超,姜存仓,刘桂东,董肖昌,吴秀文.2015.低硼胁迫对柑橘枳橙砧木生长及营养生理的影响.华中农业大学学报,34(3):64-68.
Long A,Zhang J,Yang L T,Ye X,Lai N W,Tan L L,Lin D,Chen L S.2017.Effects of low p H on photosynthesis,related physiological parameters,and nutrient profiles of citrus.Frontiers,8:185-192.
Ma Cheng-cang.1997.Effect of p H on cell membrane and cell endogenous protective system of rape seedling.Chinese Journal of Oil Crop Sciences,19(1):27-28.(in Chinese)马成仓.1997.p H对油菜幼苗细胞膜及细胞保护系统的影响.中国油料作物学报,19(1):27-28.
Malkanthi D R R,Yokoyama K,Yoshida T,Moritsugu W,Matsushita K.1995.Effects of low p H and Al on growth and nutrient uptake of several plants.Soil Science and Plant Nutrition,41(1):161-165.
Oiwa Y,Kitayama K,Kobayashi M,Matoh T.2013.Boron deprivation immediately causes cell death in growing roots of Arabidopsis thaliana(L.)Heynh.Soil Science&Plant Nutrition,59:621-627.
Pfeffer H,Dannel F,Romheld V.2001.Boron compartmentation in roots of sunflower plants of different boron status:a study using the stable isotopes 10B and 11B adopting two independent approaches.Physiology Plant,113:346-351.
Randhawa S S,Iwata M.1968.Effects of p H,calcium concentrations and sources of nitrogen on the growth and inorganic compositions of citrus seedlings in solution culture.Journal Japanese Society for Horticultural Science,37(4):23-31.
Riaz M,Yan L,Wu X W,Huaasin S,Aziz O,Imran M,Rana M S,Jiang C C.2018.Boron reduces aluminum-induced growth inhibition,oxidative damage and alterations in the cell wall components in the roots of trifoliate orange.Ecotoxicol Environ Saf,153:107-115.
Shi D H,Wang J,Hu R B,Zhou G K,O’Neill M A,Kong Y Z.2017.Boron-briged RG-Ⅱand calcium are required to maintain the pectin network of the Arabidopsis seed mucilage ultrastructure.Plant Molecular Biology,94(3):267-280.
Tavakkoli E,Lyons G,English P,Guppy C N.2011.Silicon nutrition of rice is affected by soil p H,weathering and silicon fertilisation.Journal of Plant Nutrition&Soil Science,174(3):437-446.
Vangelisti R,Viegi I,Cela Renzoni G.1995.Responses of Pinus pinea and P.pinaster seedling roots to substrata at different p H values.Annales Botanici Fennici,32:19-27.
Wu X W,Riaz M,Yan L,Du C Q,Liu Y L,Jinag C C.2017.Boron deficiency in trifoliate orange induces changes in pectin composition and architecture of components in root cell walls.Frontiers,8:1882-1890.
Xie Zhi-nan,Zhuang Yi-mei,Wang Ren-ji,Xu Wen-bao.1997.Correlation between soil p H and the contents of available nutrientsin selected soils from three kinds of orchards at subtropical zone in Fujian.Acta Horticulturae Sinica,24(3):209-214.(in Chinese)谢志南,庄伊美,王仁玑,徐文宝.1997.福建亚热带果园土壤p H值与有效态养分含量的相关性.园艺学报,24(3):209-214.
Yang Yu-hua,Yu Min,Du Chang-wen,Wu Li-shu,Wang Yun-hua.2005.Boron distribution in cell wall components in rape cultivars(Brassica napus)with different boron use efficiency.Journal of Crop,31(5):608-611.(in Chinese)杨玉华,喻敏,杜昌文,吴礼树,王运华.2005.不同硼效率油菜品种细胞壁组分中硼的分配.作物学报,31(5):608-611.
Zhang C P,Meng P,Li J Z,Wan X W.2014.Interactive effects of soil acidification and phosphorus deficiency on photosynthetic characteristics and growth in Juglans regia seedlings.China Journal of Plant Ecology,38:1345-1355.
Zhou Shan.2015.The involvement of expansins in response to water stress during leaf development in wheat[Ph.D.Dissertation]:Tai’an:Shandong Agricultural University:6-18.(in Chinese)周姗.2015.小麦叶片伸生长对干旱胁迫的响应与扩散蛋白关系研究[博士论文]:泰安:山东农业大学:6-18.
Zhu Li-qing,Wang Bo-chu,Fu Xue,Yang Xing-yan,Liu Jun-yu,Kong Jing.2013.Turgor pressure in plant cell growth.Acta Biophysica Sinica,29(8):583-593.(in Chinese)朱蠡庆,王伯初,付雪,杨兴艳,刘峻宇,孔静.2013.膨压在植物细胞生长中的作用.生物物理学报,29(8):583-593.
Arduini I,Kettner C,Godbold D L,Onnis A,Stefani A.1998.The influence of p H on root morphology and mineral content of Pinus pinaster Ait.seedlings.Plant Biosystems,132(1):3-9.
Arnon T I,Achdout H,Lieberman N,Gazit R,Gonen-Gross T,Katz G,Bar-Llan A,Bloushtain N,Lev M,Joseph A,Kedar E,Porqador A,Mandelboim O.2004.The mechanisms controlling the recognition of tumor-and virus-infected cells by NKp46.Blood,103(2):664.
Bai Ling,Zhou Yun,Zhang Xiao-ran,Song Chun-peng,Cao Ming-qing.2007.Hydrogen peroxide participates in the morphological development of Arabidopsis root regulated by abscisic acid.Chinese Science Bulletin,52(5):608-611.(in Chinese)白玲,周云,张晓然,宋纯鹏,曹鸣庆.2007.过氧化氢参与了脱落酸调控的拟南芥根形态发育.科学通报,52(5):608-611.
Bramley H,Turner N C,Turner D W,Tyerman S D.2010.The contrasting influence of short-term hypoxia on the hydraulic properties of cells and roots of wheat and lupin.Functional Plant Biology,37(3):183-193.
Dell B,Huang L.1997.Physiolgical response of plants to low boron.Plant Soil,193:103-120.
Dimitra C,David J M,Stephen C F.2014.Boron bridging of rhamnogalacturonan-Ⅱ,monitored by gel electrophoresis,occurs during polysaccharide synthesis and secretion but not post-secretion.The Plant Journal,77:534-546.
Ding Wei-hua,Wang Bin,Zhang Ying-hua,Zhou Shun-li,Wang Zhi-min.2012.Effects of p H buffers on the activities of antioxidant enzymes in winter wheat seedlings under different water status.Journal of Wheat Crop,32(5):890-894.(in Chinese)丁位华,王彬,张英华,周顺利,王志敏.2012.p H缓冲液对不同水分状态下冬小麦幼苗抗氧化酶活性的影响.麦类作物学报,32(5):890-894.
Gao Y L,Kong W L,Li B,NI Y,Yuan T,Guo L,Lin H,Fan H,Fan Y,Zhang X.2018.Fabrication and characterization of collagen-based injectable and self-crosslinkable hydrogels for cell encapsulation.Colloids&Surfaces B Biointerfaces,167:448-456.
Gijsman A J.1990.Nitrogen nutrition of Douglas-fir(Pseudotsuga menziesii)on strongly acid sandy soil.II.Proton excretion and rhizosphere p H.Plant&Soil,126(1):53-61.
Han Jia,Zhou Gao-feng,Li Qiao-hong,Liu Yong-zhong,Peng Shu-ang.2012.Effects of magnesium,iron,boron deficiency on the growth and nutrition absorption of four major citrus rootstocks.Acta Horticulturae Sinica,39(11):2105-2112.(in Chinese)韩佳,周高峰,李峤虹,刘永忠,彭抒昂.2012.缺镁、铁、硼胁迫对4个柑橘砧木生长及养分吸收的影响.园艺学报,39(11):2105-2112.
Hipps N A,Davies M J,Dodds P,Buckley G P.2005.The effects of phosphorus nutrition and soil p H on the growth of some ancient woodland indicator plants and their interaction with competitor species.Plant&Soil,271(1-2):131-141.
Hoagland R.1950.The water culture methods for growing plants without soil//California Agricultural Experiment Station Circular:347.
Hoson T.1998.Apoplast as the site of response to environmental signals.Journal of Plant Research,111:167-177.
Hu H,Brown P H,Labavitch J M.1996.Species variability in boron requirement is correlated with cell wall pectin.Journal of Experimental Botany,47:227-232.
Huang Y M,Zou Y N,Wu Q S.2017.Alleviation of drought stress by mycorrhizas is related to increased root H2O2 efflux in trifoliate orange.Scientific Reports,7:42335.
Ishii T,Matsunaga T,Hayashi N.2001.Formation of rhamnogalacturonanⅡ-borate dimer in pectin determines cell wall thickness of pumpkin tissue.Plant Physiology,126:1698-1705.
Ivanchenko M G,Os D D,Monshausen G B,Dubrovsky J G,Bednarova A,Krishnan N.2013.Auxin increases the hydrogen peroxide(H2O2)concentration in tomato(Solanum lycopersicum)root tips while inhibiting root growth.Annals of Botany,112(6):1107-1116.
Kaneko S,Ishii T,Matsunaga T.1997.A boron-rhamnogalacturonan-Ⅱcomplex from bamboo shoot cell wall.Phytochemistry,44:243-248.
Kobayashi M,Matoh T,Azuma J.1996.Two chains of rhamnoglacturonanⅡare cross-linked by borate-diol ester bonds in higher plant cell wall.Plant Physiology,110:1017-1020.
Li Xue-wen,Li Ya-lin,Yang Jin,Wu Li-shu,Xiao Hong-dong,Feng Ying-ming,Liu Jia-you,Yu Min.2016.Differences of pectin content and methylation in root cell wall of pea(Pisum sativum)cultivars with different Al tolerance.Journal of Plant Nutrition and Fertilizer,22(3):729-735.(in Chinese)李学文,李亚林,杨锦,吴礼树,萧洪东,冯英明,刘家友,喻敏.2016.豌豆不同耐铝品种根尖细胞壁果胶及其甲基酯化度的差异.植物营养与肥料学报,22(3):729-735.
Liang Mei-qing,Xue Jun,Fan Yu-lan,Li Xun,Peng Liang-zhi.2010.Studies on soil acidification of navel orange orchards in Gan-zhou City,Jiangxi Province.South China Fruits,39(4):6-8,13.(in Chinese)梁梅青,薛珺,范玉兰,李勋,彭良志.2010.赣南脐橙园土壤酸化特征研究.中国南方果树,39(4):6-8,13.
Liao Wei-biao,Huang Gao-bao,Yu Ji-hua,Zhang Mei-ling.2011.Nitric oxide and hydrogen peroxide are involved in indole-3-butyric acid-induced adventitious root formation of marigold.Acta Horticulturae Sinica,38(5):939-946.(in Chinese)廖伟彪,黄高宝,郁继华,张美玲.2011.NO和H2O2在IBA诱导万寿菊不定根形成中的作用.园艺学报,38(5):939-946.
Liu Guidong,Jiang Cuncang,Wang Yunhua.2011.Distribution of boron and its forms in young“Newhall”navel orange(Citrus sinensis Osb.)plants grafted on two rootstocks in response to deficient and excessive boron.Soil Science&Plant Nutrition,57(1):93-104.
Liu Lei-chao,Jiang Cun-cang,Liu Gui-dong,Dong Xiao-chang,Wu Xiu-wen.2015.Effects of boron stress on seedling growth and nutrition physiology of navel orange root stock.Huazhong Agricultural University,34(3):64-68.(in Chinese)刘磊超,姜存仓,刘桂东,董肖昌,吴秀文.2015.低硼胁迫对柑橘枳橙砧木生长及营养生理的影响.华中农业大学学报,34(3):64-68.
Long A,Zhang J,Yang L T,Ye X,Lai N W,Tan L L,Lin D,Chen L S.2017.Effects of low p H on photosynthesis,related physiological parameters,and nutrient profiles of citrus.Frontiers,8:185-192.
Ma Cheng-cang.1997.Effect of p H on cell membrane and cell endogenous protective system of rape seedling.Chinese Journal of Oil Crop Sciences,19(1):27-28.(in Chinese)马成仓.1997.p H对油菜幼苗细胞膜及细胞保护系统的影响.中国油料作物学报,19(1):27-28.
Malkanthi D R R,Yokoyama K,Yoshida T,Moritsugu W,Matsushita K.1995.Effects of low p H and Al on growth and nutrient uptake of several plants.Soil Science and Plant Nutrition,41(1):161-165.
Oiwa Y,Kitayama K,Kobayashi M,Matoh T.2013.Boron deprivation immediately causes cell death in growing roots of Arabidopsis thaliana(L.)Heynh.Soil Science&Plant Nutrition,59:621-627.
Pfeffer H,Dannel F,Romheld V.2001.Boron compartmentation in roots of sunflower plants of different boron status:a study using the stable isotopes 10B and 11B adopting two independent approaches.Physiology Plant,113:346-351.
Randhawa S S,Iwata M.1968.Effects of p H,calcium concentrations and sources of nitrogen on the growth and inorganic compositions of citrus seedlings in solution culture.Journal Japanese Society for Horticultural Science,37(4):23-31.
Riaz M,Yan L,Wu X W,Huaasin S,Aziz O,Imran M,Rana M S,Jiang C C.2018.Boron reduces aluminum-induced growth inhibition,oxidative damage and alterations in the cell wall components in the roots of trifoliate orange.Ecotoxicol Environ Saf,153:107-115.
Shi D H,Wang J,Hu R B,Zhou G K,O’Neill M A,Kong Y Z.2017.Boron-briged RG-Ⅱand calcium are required to maintain the pectin network of the Arabidopsis seed mucilage ultrastructure.Plant Molecular Biology,94(3):267-280.
Tavakkoli E,Lyons G,English P,Guppy C N.2011.Silicon nutrition of rice is affected by soil p H,weathering and silicon fertilisation.Journal of Plant Nutrition&Soil Science,174(3):437-446.
Vangelisti R,Viegi I,Cela Renzoni G.1995.Responses of Pinus pinea and P.pinaster seedling roots to substrata at different p H values.Annales Botanici Fennici,32:19-27.
Wu X W,Riaz M,Yan L,Du C Q,Liu Y L,Jinag C C.2017.Boron deficiency in trifoliate orange induces changes in pectin composition and architecture of components in root cell walls.Frontiers,8:1882-1890.
Xie Zhi-nan,Zhuang Yi-mei,Wang Ren-ji,Xu Wen-bao.1997.Correlation between soil p H and the contents of available nutrientsin selected soils from three kinds of orchards at subtropical zone in Fujian.Acta Horticulturae Sinica,24(3):209-214.(in Chinese)谢志南,庄伊美,王仁玑,徐文宝.1997.福建亚热带果园土壤p H值与有效态养分含量的相关性.园艺学报,24(3):209-214.
Yang Yu-hua,Yu Min,Du Chang-wen,Wu Li-shu,Wang Yun-hua.2005.Boron distribution in cell wall components in rape cultivars(Brassica napus)with different boron use efficiency.Journal of Crop,31(5):608-611.(in Chinese)杨玉华,喻敏,杜昌文,吴礼树,王运华.2005.不同硼效率油菜品种细胞壁组分中硼的分配.作物学报,31(5):608-611.
Zhang C P,Meng P,Li J Z,Wan X W.2014.Interactive effects of soil acidification and phosphorus deficiency on photosynthetic characteristics and growth in Juglans regia seedlings.China Journal of Plant Ecology,38:1345-1355.
Zhou Shan.2015.The involvement of expansins in response to water stress during leaf development in wheat[Ph.D.Dissertation]:Tai’an:Shandong Agricultural University:6-18.(in Chinese)周姗.2015.小麦叶片伸生长对干旱胁迫的响应与扩散蛋白关系研究[博士论文]:泰安:山东农业大学:6-18.
Zhu Li-qing,Wang Bo-chu,Fu Xue,Yang Xing-yan,Liu Jun-yu,Kong Jing.2013.Turgor pressure in plant cell growth.Acta Biophysica Sinica,29(8):583-593.(in Chinese)朱蠡庆,王伯初,付雪,杨兴艳,刘峻宇,孔静.2013.膨压在植物细胞生长中的作用.生物物理学报,29(8):583-593.