细胞外基质的硬度与细胞生物学功能的研究进展
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摘要
细胞外基质是细胞赖以生存的环境,细胞外基质中物质和代谢的不平衡导致细胞外基质硬度的变化,从而能够影响细胞的一系列生物学功能。本文阐述了近期关于细胞外基质的改变与力学相关性的研究,以及其对细胞的迁移、细胞核形态的改变、细胞分化、基因转移、细胞的信号转导方面的影响。
Cell survival environment is extracellular matrix,and Unbalanced metabolism of it could have effect on the biological function.Several recent reports have shown that cells modulate themselves in respond to their surrounding environment,including mechanical correlation,migration,modulation of nuclear shape,cell differentiation,gene transfer,modulation of signal transduction.
Cell survival environment is extracellular matrix,and Unbalanced metabolism of it could have effect on the biological function.Several recent reports have shown that cells modulate themselves in respond to their surrounding environment,including mechanical correlation,migration,modulation of nuclear shape,cell differentiation,gene transfer,modulation of signal transduction.
引文
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[2]王红兵,林雨,杨力,等.基底硬度和表面形貌对细胞生物学行为的影响[J].细胞生物学杂志,2009,31(5):608-614.
[3]刘永全.整合素与信号转导[J].国外医学:免疫学分册,2000,23(1):35-39.
[4]高敏,刘肖珩,孙亨,等.FAK通过黏着斑蛋白调控血管内皮细胞迁移的研究[J].生物医学工程学杂志,2013,30(3):567-571.
[5]Yip AK,Iwasaki K,Ursekar C,et al.Cellular response to substrate rigidity is governed by either stress or strain[J].Biophys J,2013,104(1):19-29.
[6]De R,Zemel A,Safran SA.Do cells sense stress or strain?Measurement of cellular orientation can provide a clue[J].Biophys J,2008,94(5):L29-L31.
[7]Borau C,Kim T,Bidone T,et al.Dynamic mechanisms of cell rigidity sensing:insights from a computational model of actomyosin networks[J].PLoS One,2012,7(11):e49 174.
[8]舒咬根,欧阳钟灿.生物分子马达[J].物理,2007,36(10):735-741.
[9]Zhong Y,Ji B.Impact of cell shape on cell migration behavior on elastic substrate[J].Biofabrication,2013,5(1):015011.
[10]Plotnikov SV,Pasapera AM,Sabass B,et al.Force fluctuations within focal adhesions mediate ECM-rigidity sensing to guide directed cell migration[J].Cell,2012,151(7):1 513-1 527.
[11]Beck JN,Singh A,Rothenberg AR,et al.The independent roles of mechanical,structural and adhesion characteristics of 3D hydrogels on the regulation of cancer invasion and dissemination[J].Biomaterials,2013,34(37):9 486-9 495.
[12]Juin A,Planus E,Guillemot F,et al.Extracellular matrix rigidity controls podosome induction in microvascular endothelial cells[J].Biol Cell,2013,105(1):46-57.
[13]Simon DN,Wilson KL.The nucleoskeleton as a genome-associated dynamic'network of networks'[J].Nat Rev Mol Cell Biol,2011,12(11):695-708.
[14]Lovett DB,Shekhar N,Nickerson JA,et al.Modulation of nuclear shape by substrate rigidity[J].Cell Mol Bioeng,2013,6(2):230-238.
[15]Lo CM,Wang HB,Dembo M,et al.Cell movement is guided by the rigidity of the substrate[J].Biophys J,2000,79(1):144-152.
[16]Dado D,Sagi M,Levenberg S,et al.Mechanical control of stem cell differentiation[J].Regenerative Medicine,2012,7(1):101-116.
[17]Saha S,Ji L,De Pablo JJ,et al.TGFbeta/activin/nodal pathway in inhibition of human embryonic stem cell differentiation by mechanical strain[J].Biophys J,2008,94(10):4 123.
[18]Sun Y,Villa-Diaz LG,Lam RHW,et al.Mechanics regulates fate decisions of human embryonic stem cells[J].PLoS One,2012,7(5):e37178.
[19]Chu C,Kong H.Interplay of cell adhesion matrix stiffness and cell type for non-viral gene delivery[J].Acta Biomaterialia,2012,8(7):2 612-2 619.
[20]谷佳,韩涛,赵宁,等.蛋白酪氨酸磷酸酶Shp2与肿瘤[J].中国肿瘤生物治疗杂志,2013,20(5):623-628.
[21]罗虎,周向东.SHP2及其在乳腺癌发生发展中的作用[J].生命的化学,2010,30(6):833-837.
[22]Lee KA,Chen QK,Lui C,et al.Matrix compliance regulates Rac1blocalization,NADPH oxidase assembly,and epithelial-mesenchymal transition[J].Mol Biol Cell,2012,23(20):4 097-4 108.
[23]Acloque H,Adams MS,Fishwick K,et al.Epithelialmesenchymal transitions:the importance of changing cell state in development and disease[J].J Clin Invest,2009,119(6):1 438-1 449.
[24]周嘉黎,张玉,师少军.Rac1的生物学作用及其调控机制研究进展[J].中国药师,2014,17(1):146-149.
[25]章硕,姜浩,苏琦.Rac1与肿瘤的研究进展[J].国际病理科学与临床杂志,2011,31(5):410-414.
[26]沈园园,潘跃银.肥胖与乳腺癌关系的研究进展[J].临床肿瘤学杂志,2014,19(1):72-77.
[27]Ghosh S,Ashcraft K,Jahid MJ,et al.Regulation of adipose oestrogen output by mechanical stress[J].Nat Commun,2013,4:1 821.