基于电导率快速测定豆类细胞破壁率方法的研究
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摘要
为了建立一种通过电导率,能够快速准确检测豆类细胞破壁率的方法。通过测定不同处理程度的四种豆子的蛋白含量、电导率,考察了四种豆子水溶性蛋白释放量与电导率之间的关系。研究了豆浆用量、外渗时间、测定温度对破壁率的影响。利用建立的评价方法,分别测定了四种常见豆子在破壁料理机豆浆程序下的破壁率。结果表明:四种豆子破壁过程中水溶性蛋白的释放量与电导率相关性较高(R2≥0.8995),豆浆用量和外渗时间对于破壁率的测定结果影响不显著(p>0.05),测定温度对于破壁率的影响非常显著(p<0.01),测定温度在25℃左右时破壁率的测定结果较为稳定。四种豆子在新型破壁料理机豆浆程序下的破壁率达到90%左右。结论,利用电导率快速评价豆类细胞破壁率的方法,具有一定的可行性。
A method for rapid and accurate determination of wall-broken rate of beans cells by conductivity was established in this study. The relationship between water-soluble proteins of four kinds of beans and conductivity was investigated by measuring the protein content and conductivity of four kinds of beans with different degrees of treatment, and the effects of soymilk dosage, extravasation time and measure temperature on the wall-broken rate were also studied. The wall-broken rate of four common beans under the soymilk program of high speed blender was determined by using the established evaluation method. Results suggested that the correlation between the concentration of water-soluble proteins in the four common beans and the conductivity was significant(R2≥0.8995), and the soymilk dosage and extravasation time had no significant influence on the determination of wall-broken rate(p>0.05). The effect of measure temperature on wall-broken rate was very significant(p<0.01), and the wall-broken rate was relatively stable at 25 oC. The wall-broken rate of four common beans reached about 90% by using the new high speed blender. Consequently, it was feasible to quickly evaluate the wall-broken rate of beans cells by conductivity.
A method for rapid and accurate determination of wall-broken rate of beans cells by conductivity was established in this study. The relationship between water-soluble proteins of four kinds of beans and conductivity was investigated by measuring the protein content and conductivity of four kinds of beans with different degrees of treatment, and the effects of soymilk dosage, extravasation time and measure temperature on the wall-broken rate were also studied. The wall-broken rate of four common beans under the soymilk program of high speed blender was determined by using the established evaluation method. Results suggested that the correlation between the concentration of water-soluble proteins in the four common beans and the conductivity was significant(R2≥0.8995), and the soymilk dosage and extravasation time had no significant influence on the determination of wall-broken rate(p>0.05). The effect of measure temperature on wall-broken rate was very significant(p<0.01), and the wall-broken rate was relatively stable at 25 oC. The wall-broken rate of four common beans reached about 90% by using the new high speed blender. Consequently, it was feasible to quickly evaluate the wall-broken rate of beans cells by conductivity.
引文
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[12]林国辉,林丽云.浅议电导测量的影响因素[J].计量与测试技术,2014,41(7):37-38LIN Guo-hui,LIN Li-yun.Discussion on factors influencing the conductance measurement[J].Metrology&Measurement Technique,2014,41(7):37-38
[2]赵洁胜,魏联,虞燕萍,等.灵芝孢子粉破壁率的检验方法研究进展[J].中国医药指南,2013,5:431-434ZHAO Jie-sheng,WEI Lian,YU Yan-ping,et al.Research progress on the test method of Reishi mushroom powder broken rate[J].Guide of China Medicine,2013,5:431-434
[3]叶英响,冯超,翁夏蒙,等.六味地黄丸粉体粒径与其破壁率和溶出度的相关性研究[J].中草药,2016,47(12):2108-2112YE Ying-xiang,FENG Chao,WENG Xia-meng,et al.Correlation of power particle size,cell wall-breaking rate and in vitro dissolution rate of Liuwei Dihuang Pill[J].Chinese Traditional and Herbal Drugs,2016,47(12):2108-2112
[4]宋忠兴,赵鹏,唐志书,等.4种中药微细粉体显微特征及细胞破壁率的测定[J].中国现代中药,2017,19(2):246-249SONG Zhong-xing,ZHAO Peng,TANG Zhi-shu,et al.Study on microscopic feature and wall-broken rate of four kinds of traditional chinese medicine micro-fine powders[J].Modern Chinese Medicine,2017,19(2):246-249
[5]林旭东,潘巨忠,凌建刚.豆浆的保健功能及研究进展[J].现代农业科技,2007,24:146-146LIN Xu-dong,PAN Ju-zhong,LING Jian-gang,et al.The health functions and research progress of soymilk[J].Modern Agricultural Science and Technology,2007,24:146-146
[6]薛伟明,张效林,刘袖洞,等.电导率测定法在植物有效成分提取过程中的应用研究[J].现代化工,1998,8:21-24XUE Wei-ming,ZHANG Xiao-lin,LIU Xiu-dong,et al.Application of specific conductance method in the extraction of effective components from plants[J].Modern Chemical Industry,1998,8:21-24
[7]孙冬梅,陈卉,王洛临,等.电导率测定法在中药有效成分提取过程中的应用研究[J].中成药,2015,37(12):2686-2690.SUN Dong-mei,CHEN hui,WANG Luo-lin,et al.Application of specific conductance method in the extraction of effective constituents from traditional Chinese medicines[J].Chinese Traditional Patent Medicine,2015,37(12):2686-2690
[8]GB/T 5497-1985,粮食、油料检验水分测定法[S]
[9]GB/T 5497-1985,Inspection of grain and oilseeds Methods for determination of moisture content[S]
[10]GB 50095-2010,食品安全国家标准食品中蛋白质的测定[S]GB 50095-2010,National food safety standard Determination of protein in foods[S]
[11]王孝平,邢树礼.考马斯亮蓝法测定蛋白含量的研究[J].天津化工,2009,23(3):40-42WANG Xiao-ping,XING Shu-li.Determination of protein guanitation using the method of coomassie brilliant blue[J].Tianjin Chemical Industry,2009,23(3):40-42
[12]林国辉,林丽云.浅议电导测量的影响因素[J].计量与测试技术,2014,41(7):37-38LIN Guo-hui,LIN Li-yun.Discussion on factors influencing the conductance measurement[J].Metrology&Measurement Technique,2014,41(7):37-38