基于文献计量分析细胞破壁技术的研究现状与发展趋势
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摘要
为分析细胞破壁技术的研究现状与发展趋势,对中国知识资源总库(CNKI)和中国专利数据总库采用主题词关联的模糊方式进行检索,所得检索数据用文献计量方法进行分析。结果表明:细胞破壁技术研究方兴未艾,特别是在代表着自主知识产权的专利文献进入21世纪后得到迅猛发展;细胞破壁技术在医药、生物、农业、食品和化工等诸多领域的应用日益拓展与完善;多地多学科的协同与联合效应正在发生,各研究单位的特色也越发鲜明,一个百花齐放的研究局面正在形成。同时,文章也指出了细胞破壁技术还面临着难以均匀恒定地维持低温或超低温环境、智能化专用设备及其配套技术近乎空白等难题,这都需要加强其基础研究的深度、突破技术瓶颈、拓展其应用广度等,以开创出细胞破壁技术的新春天。
In order to analyze the current situation and development trend of cell wall breaking technology in China,the fuzzy method of key word correlation was used to search in the China National Knowledge Infrastructure (CNKI) and China's patent database,and the retrieved data were analyzed by the method of Bibliometrics.The results showed that the research on cell wall breaking technology in China was in the ascendant and obtained rapid development especially after patent documents representing independent intellectual property rights showed up in the 21st century. The application of cell wall breaking technology in many fields such as medicine,biology,agriculture,food and chemical industry was increasingly expanded and perfected.Synergy and combined effect of multi field and multi discipline was taking place,and the characteristics of research units were becoming more and more distinct.A prosperous research situation was taking shape.At the same time,the article also pointed out that the cell wall breaking technology was still facing difficulties in maintaining a low temperature or ultra-low temperature of the environment uniformly and constantly,and intelligent special equipment and its supporting technology almost remain,etc.All these need efforts to strengthen the depth of basic research,break through the technical bottleneck,expand application breadth and keep going deeply into it,so as to create a new spring of cell wall breaking technology.
In order to analyze the current situation and development trend of cell wall breaking technology in China,the fuzzy method of key word correlation was used to search in the China National Knowledge Infrastructure (CNKI) and China's patent database,and the retrieved data were analyzed by the method of Bibliometrics.The results showed that the research on cell wall breaking technology in China was in the ascendant and obtained rapid development especially after patent documents representing independent intellectual property rights showed up in the 21st century. The application of cell wall breaking technology in many fields such as medicine,biology,agriculture,food and chemical industry was increasingly expanded and perfected.Synergy and combined effect of multi field and multi discipline was taking place,and the characteristics of research units were becoming more and more distinct.A prosperous research situation was taking shape.At the same time,the article also pointed out that the cell wall breaking technology was still facing difficulties in maintaining a low temperature or ultra-low temperature of the environment uniformly and constantly,and intelligent special equipment and its supporting technology almost remain,etc.All these need efforts to strengthen the depth of basic research,break through the technical bottleneck,expand application breadth and keep going deeply into it,so as to create a new spring of cell wall breaking technology.
引文
[1]修志龙,姜炜,苏志国.细胞破碎技术的研究进展和发展方向[J].化工进展,1994(1):15-21.
[2]孔祥佩.中药细胞破壁技术在畜禽,水产领域中的应用[J].福建畜牧兽医,2001,23(2):51-52.
[3]李洪德.用破壁方法增大泥浆钻孔出水量[J].探矿工程(岩土钻掘工程),1979(6):33-34.
[4]吴灿明,王立杰,林敬明,等.灵芝孢子粉破壁方法优化及其免疫调节和抗肿瘤机制研究[J].今日药学,2017(5):307-311.
[5]成金乐,赖智填,陈炜璇,等.中药破壁饮片---传统中药饮片的传承和创新[J].世界科学技术:中医药现代化,2016,18(9):1546-1552.
[6]马福敏,刘玉玲.复合破壁方法对蜂花粉破壁率及其主要功能性成分的影响[J].食品与发酵工业,2016,42(5):184-186.
[7]赵素芬.破壁灵芝孢子粉提取物抑制人卵巢上皮性癌细胞生长、转移及逆转顺铂耐药的研究[D].石家庄:河北医科大学,2010.
[8]宋忠兴,赵鹏,唐志书,等.4种中药微细粉体显微特征及细胞破壁率的测定[J].中国现代中药,2017,19(2):246-249.
[9]刘明源,张美硕,杨艺,等.6种啤酒酵母细胞破壁处理方式的对比分析[J].现代农业科技,2012(6):340-340.
[10]苗帅,项云峰.破壁料理机的细胞壁破壁效果评价方法的研究[J].日用电器,2017(4):16-20.
[11]邱均平.信息计量学(四):第四讲文献信息离散分布规律---布拉德福定律[J].情报理论与实践,2000(4):315-320.
[12]张代佳,刘传斌,修志龙,等.微波技术在植物胞内有效成分提取中的应用[J].中草药,2000(9):86-87.
[13]杨翠竹,李艳,阮南,等.酵母细胞破壁技术研究与应用进展[J].食品科技,2006,31(7):138-142.
[14]唐维,张星海.花粉破壁方法的研究进展[J].食品与发酵工业,2003,29(2):86-92.
[15]关天增,雷敬卫,郑艳丽.浅谈超微粉碎[J].中国中药杂志,2002(7):22-24.
[16]林志彬,王鹏云.灵芝孢子及其活性成分的药理研究[J].北京大学学报:医学版,2006(5):541-547.
[17]陈体强,朱培根,蔡云,等.原木灵芝孢子研究---(Ⅳ)未破壁孢子与破壁孢子的比较[J].食用菌学报,1999,6(3):21-26.
[18]吴君,黄萍,成金乐,等.党参破壁粉粒增强免疫功能及抗疲劳作用的研究[J].中国实验方剂学杂志,2011,17(4):179-181.
[19]张国祖,王伟伟,郭振环.超微粉碎技术在兽用中药现代化生产上的应用和前景[J].中兽医医药杂志,2013,32(1):72-74.
[20]于博,张瑶,吴进菊,等.超低温冻融联合超声对生姜中姜辣素提取的影响[J].江苏农业科学,2017,45(20):206-209.
[21]任占强.超声波处理活性污泥细胞破壁脱水研究[J].环境科学与管理,2014,39(6):105-108.
[22]马宏亮,成金乐,乔卫林,等.中药破壁饮片全产业链质量保证体系与溯源体系建设[J].世界科学技术:中医药现代化,2017,19(1):120-126.
[23]杨丽娜,鞠俭奎.中药饮片质量标准规范化进程与思考[J].辽宁中医药大学学报,2010(3):174-175.
[24]刘明磊.微藻细胞空化破壁技术研究[D].青岛:中国石油大学(华东),2014.
[2]孔祥佩.中药细胞破壁技术在畜禽,水产领域中的应用[J].福建畜牧兽医,2001,23(2):51-52.
[3]李洪德.用破壁方法增大泥浆钻孔出水量[J].探矿工程(岩土钻掘工程),1979(6):33-34.
[4]吴灿明,王立杰,林敬明,等.灵芝孢子粉破壁方法优化及其免疫调节和抗肿瘤机制研究[J].今日药学,2017(5):307-311.
[5]成金乐,赖智填,陈炜璇,等.中药破壁饮片---传统中药饮片的传承和创新[J].世界科学技术:中医药现代化,2016,18(9):1546-1552.
[6]马福敏,刘玉玲.复合破壁方法对蜂花粉破壁率及其主要功能性成分的影响[J].食品与发酵工业,2016,42(5):184-186.
[7]赵素芬.破壁灵芝孢子粉提取物抑制人卵巢上皮性癌细胞生长、转移及逆转顺铂耐药的研究[D].石家庄:河北医科大学,2010.
[8]宋忠兴,赵鹏,唐志书,等.4种中药微细粉体显微特征及细胞破壁率的测定[J].中国现代中药,2017,19(2):246-249.
[9]刘明源,张美硕,杨艺,等.6种啤酒酵母细胞破壁处理方式的对比分析[J].现代农业科技,2012(6):340-340.
[10]苗帅,项云峰.破壁料理机的细胞壁破壁效果评价方法的研究[J].日用电器,2017(4):16-20.
[11]邱均平.信息计量学(四):第四讲文献信息离散分布规律---布拉德福定律[J].情报理论与实践,2000(4):315-320.
[12]张代佳,刘传斌,修志龙,等.微波技术在植物胞内有效成分提取中的应用[J].中草药,2000(9):86-87.
[13]杨翠竹,李艳,阮南,等.酵母细胞破壁技术研究与应用进展[J].食品科技,2006,31(7):138-142.
[14]唐维,张星海.花粉破壁方法的研究进展[J].食品与发酵工业,2003,29(2):86-92.
[15]关天增,雷敬卫,郑艳丽.浅谈超微粉碎[J].中国中药杂志,2002(7):22-24.
[16]林志彬,王鹏云.灵芝孢子及其活性成分的药理研究[J].北京大学学报:医学版,2006(5):541-547.
[17]陈体强,朱培根,蔡云,等.原木灵芝孢子研究---(Ⅳ)未破壁孢子与破壁孢子的比较[J].食用菌学报,1999,6(3):21-26.
[18]吴君,黄萍,成金乐,等.党参破壁粉粒增强免疫功能及抗疲劳作用的研究[J].中国实验方剂学杂志,2011,17(4):179-181.
[19]张国祖,王伟伟,郭振环.超微粉碎技术在兽用中药现代化生产上的应用和前景[J].中兽医医药杂志,2013,32(1):72-74.
[20]于博,张瑶,吴进菊,等.超低温冻融联合超声对生姜中姜辣素提取的影响[J].江苏农业科学,2017,45(20):206-209.
[21]任占强.超声波处理活性污泥细胞破壁脱水研究[J].环境科学与管理,2014,39(6):105-108.
[22]马宏亮,成金乐,乔卫林,等.中药破壁饮片全产业链质量保证体系与溯源体系建设[J].世界科学技术:中医药现代化,2017,19(1):120-126.
[23]杨丽娜,鞠俭奎.中药饮片质量标准规范化进程与思考[J].辽宁中医药大学学报,2010(3):174-175.
[24]刘明磊.微藻细胞空化破壁技术研究[D].青岛:中国石油大学(华东),2014.