高温胁迫下坛紫菜(Pyropia haitanensis)对无机碳的利用
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摘要
采用藻类二氧化碳浓缩机制(CO2Concentrating Mechanism,CCM)关键酶胞外碳酸酐酶(periplasmic Carbonic Anhydrase,pCA)的抑制剂AZ(acetazolamide)、DIDS(4’4’-diisothiocyanatosilbene-2,2-disulfonic acid)和SITS(4-acetamido-4’-isothiocyano-2,2’-stibene-disulfonate)等对不同生长阶段的坛紫菜(Pyropia haitanensis)叶状体进行处理,利用pH-drift技术研究了高温胁迫下坛紫菜利用无机碳的特点。结果表明:(1)坛紫菜主要依赖pCA利用HCO3,且能力较强;(2)高温抑制坛紫菜吸收利用无机碳,温度越高影响越严重;(3)坛紫菜未性成熟组叶片对无机碳的利用能力远大于成熟组,但对高温胁迫的抗性低于成熟组。高温造成藻体细胞膜系统损伤和脂肪酸成分变化或许是高温抑制藻类CCM的原因之一。
Mechanisms of inorganic carbon utilization by red seaweed Pyropia haitanensis(Rhodophyta) were studied by pH-drift technique in a closed system. Inhibitors of periplasmic Carbonic Anhydras(pCA), the key enzyme in CO2 Concentrating Mechanism(CCM), such as AZ,(acetazolamide), DIDS(4'4'-diisothiocyanatosilbene-2, 2-disulfonic acid) and SITS(4-acetamido-4'-isothiocyano-2, 2'-stibene-disulfonate) were applied to detect the CCM in P. haitanensis under heat stress. The results indicate that:(1) P. haitanensis had a strong ability to utilize HCO3, which was mainly conducted by pCA;(2) Heat stress inhibited the utilization of inorganic carbon(Ci) of P. haitanensis, and the higher the temperature, the lower utilization;(3) The thalli of the immature group had higher CCM efficiency and lower heat tolerance than the mature groups. The breakdown of membrane systems and the changes in the components of aliphatic acid in algae cells might be the reason of the CCM inhibition under heat stress.
Mechanisms of inorganic carbon utilization by red seaweed Pyropia haitanensis(Rhodophyta) were studied by pH-drift technique in a closed system. Inhibitors of periplasmic Carbonic Anhydras(pCA), the key enzyme in CO2 Concentrating Mechanism(CCM), such as AZ,(acetazolamide), DIDS(4'4'-diisothiocyanatosilbene-2, 2-disulfonic acid) and SITS(4-acetamido-4'-isothiocyano-2, 2'-stibene-disulfonate) were applied to detect the CCM in P. haitanensis under heat stress. The results indicate that:(1) P. haitanensis had a strong ability to utilize HCO3, which was mainly conducted by pCA;(2) Heat stress inhibited the utilization of inorganic carbon(Ci) of P. haitanensis, and the higher the temperature, the lower utilization;(3) The thalli of the immature group had higher CCM efficiency and lower heat tolerance than the mature groups. The breakdown of membrane systems and the changes in the components of aliphatic acid in algae cells might be the reason of the CCM inhibition under heat stress.
引文
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孙猛,吕德国,刘威生,2009.杏属植物光合作用研究进展.果树学报,26(6):878—885
何培民,徐春,於新建等,2002.莱茵藻胞外碳酸酐酶分子定位与活性诱导.植物学通报,19(3):317—321
邹定辉,高坤山,2001.大型海藻类光合无机碳利用研究进展.海洋通报,20(5):83—90
张小龙,杨锐,仪茜茜等,2010.坛紫菜胞质型抗坏血酸过氧化物酶基因的克隆及特征分析,海洋学报,32(5):165—174
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Mittler R,Vanderauwera S,Gollery M et al,2004.Reactive oxygen gene network of plants.Trends in Plant Science,10:490—496
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Nimer N A,Wsrren M,Merrett M J,1998.The regulation of photosynthetic rate and activation of extacellular carbonic anhydrase under CO2limiting conditions in the marine diatom Skeletonema costatum.Plant Cell Environ,21:805—812
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Setimela P S,Chitalu Z,Jonazi J et al,2005.Environmental classification of maize testing sites in the SADC region and its implication for collaborative maize breeding strategies in the subcontinent.Euphytica,145:123—132
Stephens E,Ian L Ross,Jan H Mussgnug et al,2010.Future prospects of microalgal biofuel production systems.Trends in Plant Science,15:554—564
Wang Y J,Duanmu D Q,Martin H S,2011.Carbon dioxide concentrating mechanism in Chlamydomonas reinhardtii:inorganic carbon transport and CO2recapture.Photosynth Res,109:115—122
Yang R,Liu W,Zhang X L et al,2013.Sequences of Mn-sod gene from Pyropia haitanensis and its expression under heat shock.Botanica Marina,56(3):249—259
Zou D H,Xia J R,Yang Y F,2004.Photosynthetic use of exogenous inorganic carbon in the agarophyte Gracilaria lemaneiformis(Rhodophyta).Aquaculture,237(1—4):421—431
孙猛,吕德国,刘威生,2009.杏属植物光合作用研究进展.果树学报,26(6):878—885
何培民,徐春,於新建等,2002.莱茵藻胞外碳酸酐酶分子定位与活性诱导.植物学通报,19(3):317—321
邹定辉,高坤山,2001.大型海藻类光合无机碳利用研究进展.海洋通报,20(5):83—90
张小龙,杨锐,仪茜茜等,2010.坛紫菜胞质型抗坏血酸过氧化物酶基因的克隆及特征分析,海洋学报,32(5):165—174
张宗申,利容千,王建波,2001.Ca预处理对热胁迫下辣椒叶内细胞中Ca-ATP酶活性的影响.植物生理学报,27(6):451—454
岳国峰,周百成,2000.条斑紫菜对无机碳的利用.海洋与湖沼,31(3):246—251
骆其君,裴鲁青,潘双叶等,2002.坛紫菜自由丝状体对无机碳的利用.水产学报,26(5):477—480
韩博平,韩志国,付翔,2003.藻类光合作用机理与模型.北京:科学出版社,217—252
Aminaka R,Taira Y,Kashino Y et al,2006.Acclimation to the growth temperature and thermosensitivity of Photosystem II in a mesophilic cyanobacterium,Synechocystis sp.PCC6803.Plant Cell Physiol,47:1612—1621
Axelsson L,Ryberg H,Beer S,1995.Two modes of bicarbonate utilization in the marine green macroalga Ulva lactuca.Plant Cell Environ,18:439—445
Díaz-Almeyda E,ThoméM P E,Hafid Eli et al,2011.Differential stability of photosynthetic membranes and fatty acid composition at elevated temperature in Symbiodinium.Coral Reefs,30:217—225
Finkel Z V,Beardall J,Flynn K J et al,2010.Phytoplankton in a changing world:cell size and elemental stoichiometry.J Plankt Res,32:119—137
Gao K,Ji Y,Aruga Y,1999.Relationship of CO2concentrations to photosynthesis of intertidal macroalgae during emersion.Hydrobiologia,355—359
Ghoshal D,Husic H D,Goyal A,2002.Dissolved inorganic carbon concentration mechanism in Chlamydomonas moewusii.Plant Physiol Biochem,40(4):299—305
Johnston A M,Maberly S,Raven J A,1992.The acquisition of inorganic carbon by four red macroalgae from different habitats.Oecologia,92:317—326
Larsson C,Axelsson L,1999.Bicarbonate uptake and utilization in marine macroalgae.European Journal of Phycology,34(1):79—86
Mercado J M,Gordillo F J L,Figueroa F L et al,1998.External carbonic anhydrase and affinity for inorganic carbon in intertidal macroalgae.Journal of Experimental Marine Biology and Ecology,221:209—220
Mercado J M,Niell F X,1999.Carbonic anhydrase activity and use of HCO3in Bostrychia scorpioides(Ceramiales,Rhodophyceae).European Journal of Phycology,34:13—19
Mittler R,Vanderauwera S,Gollery M et al,2004.Reactive oxygen gene network of plants.Trends in Plant Science,10:490—496
Munoz R,Guieysse B,2006.Algal-bacterial processes for the treatment of hazardous contaminants:a review.Water Research,40:2799—2815
Nimer N A,Wsrren M,Merrett M J,1998.The regulation of photosynthetic rate and activation of extacellular carbonic anhydrase under CO2limiting conditions in the marine diatom Skeletonema costatum.Plant Cell Environ,21:805—812
Raven J A,Cockell C S,De La Rocha C L,2008.The evolution of inorganic carbon concentrating mechanisms in photosynthesis.Philos Trans R Soc B,363:2641—2650
Setimela P S,Chitalu Z,Jonazi J et al,2005.Environmental classification of maize testing sites in the SADC region and its implication for collaborative maize breeding strategies in the subcontinent.Euphytica,145:123—132
Stephens E,Ian L Ross,Jan H Mussgnug et al,2010.Future prospects of microalgal biofuel production systems.Trends in Plant Science,15:554—564
Wang Y J,Duanmu D Q,Martin H S,2011.Carbon dioxide concentrating mechanism in Chlamydomonas reinhardtii:inorganic carbon transport and CO2recapture.Photosynth Res,109:115—122
Yang R,Liu W,Zhang X L et al,2013.Sequences of Mn-sod gene from Pyropia haitanensis and its expression under heat shock.Botanica Marina,56(3):249—259
Zou D H,Xia J R,Yang Y F,2004.Photosynthetic use of exogenous inorganic carbon in the agarophyte Gracilaria lemaneiformis(Rhodophyta).Aquaculture,237(1—4):421—431